Browse > Article
http://dx.doi.org/10.9799/ksfan.2011.24.1.094

The Antioxidative Effects and Isolation and Characterization of the Extracts from Morus alba L.  

Jeon, Ye-Sook (Dept. of Food and Nutrition, Chungwoon University)
Kim, Mi-Won (Dept. of Food and Nutrition, Chungwoon University)
Publication Information
The Korean Journal of Food And Nutrition / v.24, no.1, 2011 , pp. 94-100 More about this Journal
Abstract
This study identified the formula of the antioxidant substance separated from the ethyl acetate and the butanol extract and tested the antioxidant properties with the electron donating ability(EDA). Each phase with the fractionated methanol extract from mulberry leaf was screened in advance for the antioxidant substance with EDA. As the result, activity appeared in the ethyl acetate and butanol phase and the antioxidant component was separated. As the consequence, 2 components from the ethyl acetate phase and 1 from the butanol phase were separated, among which the structures of the components from ethyl acetate were determined by wogonin and linarin, whereas the structure of the component from the butanol phase was determined by pectolinarin. In the screening of antioxidant activity by the scavenging effect of the DPPH radical, the wogonin and linarin components from ethyl acetate phase showed more powerful antioxidant property than the component from butanol. The results from this study indicate that the chemical compound separated from the ethyl acetate extract has more powerful antioxidant property than the one separated from the butanol extract. The components separated from the ethyl acetate extract were wogonin and linarin, which are flavonoids, whereas the component from butanol was pectolinarin. Therefore, this study suggested that the feasibility of mulberry leaf as a functional food additive and its value as a natural antioxidant is very high.
Keywords
mulberry leaf; antioxidant; DPPH radical; wogonin; linarin; pectolinarin;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Lee HS, Chung KS, Kim SY, Ryu KS, Lee WC. 1998. Effect of several sericultural products on blood glucose lowering for Alloxan-induced hyperglycemic mice. Korean J Seric Sci 40:38-42
2 Lee JM, Kim JA, Lee MJ. 2002. Sensory and physicochemical attributes of boogags using mulberry leaf. Korean J Dietary Culture 17:103-110
3 Marlham. 1982. Techniques of Flavonoid Identification. Academic Press. London
4 Park BH, Back KY, Lee SI, Kim SD. 2008. Quality and antioxidative characteristics of Cudrania tricuspidata leaves tea. Korean J Food Preserv 15:461-468
5 Takido M, Aimi M, Takahashi S, Yamanouchi S, Torii H, Dohi S. 1975. Studies on the constituents in the water extracts of crude drugs. I. On the roots of Scutellaria baicalensis georgi(Wogon)1. Yakugaku Zasshi 95:108-113
6 Toshiko S, Ikuyo T, Noriko H, Taketoshi K. 1994. Thermal stability of chiorophyll in green leaves. 調理科學 27:126-131
7 Kim HB, Kang CK, Sung GB, Kang SW, Lee JR. 2007. Antioxid ative capacity of mulberry leaf and its tea. Korean J Seric Sci 49:18-23
8 Kim HB, Koh SH, Seok YS. 2006. Anti-oxidative effect of 'Cheongilppong' with mulberry leaves according to different collection areas and some kinds of mulberry branches. Korean J Seric Sci 48:41-45
9 Kim HB, Lee YW, Lee WJ, Moon JY. 2001. Physiological effects and sensory characteristics of mulberry fruit wine with chongilppong. Korean J Seris Sci 43:16-20
10 Kim HB. 2005. Antioxidant capacity analysis of water-soluble substances according to maturity stages in yield-type mulberry leaves and fruits collected from Kang-Won province. Korean J Seric Sci 47:93-98
11 Kim SS, Jeong MH, Seo YC, Kim JS, Kim NS, Woon WB, Ahn JH, Hwang B, Park DS, Park SJ, Lee HY. 2010. Comparison of antioxidant activities by high pressure extraction of Codonopsis lanceolata from different production areas. Korean J Medicinal Crop Sci 18:248-254
12 Jeong CH, Joo OS, Shim KH. 2002. Chemical components and physiological activities of young mulberry stem. J. Korea Food Preserv 9:228-233
13 Lee HB, Kwak JH, Zee OP, Yoo SJ. 1994. Flavonoids from Circum rhinoceros. Arch Pharm Res 17:273-277   DOI   ScienceOn
14 Inagaki I, Hisada S, Shima K. 1971. Studies on the constituents of anodendron affine durce. I. Isolation of wogonin, dambonitol, sucrose and some other components from stems. Yakugaku Zasshi 91:1133-1136
15 Ishida H, Umino T, Tsuji K, Kosuge T. 1987. Studies on antihemorrhagic substances in herbs classified as hemostatics in chinese medicine. VII. On the antihemorrhagic principle in Cirsium japonicum DC. Chem Pharm Bull 35:861-864   DOI   ScienceOn
16 Ju MJ, Kwon JH, Kim HK. 2009. Physiological activities of mulberry leaf and fruit extracts with different extraction conditions. Korean J Food Preserv 16:442-448
17 K. R. Markham. 1982. Techniques of Flavonoid Identification. Academic Press, pp.1-45. London
18 Chang HJ, Noh KL. 2000. Screening of electron donating ability antibacterial and nitrits scavenging effect of some herbal extracts. Korean J Food Cookery Sci 16:372-377
19 Kang PD, KimJW, Sohn BH, Kim KY, JungIY, Kim MJ, Ryu KS. 2006. Accumulating pattern of a-glucosidase inhibitor various silkworom varities. J Seric. Sci 48:25-27
20 Karen J, Pamela J. 1991. Extraction and identification of antioxidants in oats. JAOCS 86:365-370
21 Cho YJ, Ju IS, Kim BO, Kim JH, Lee BG. 2007. The antimicrobial activity against Helicobacter pylori and antioxidant effect from the extracts of mulberry leaves. J Korean Soc Appl Biol Chem 50:334-343
22 Dietz BM, Kang YH, Liu G, Eggler AL, Yao P, Chadwick LR, Pauli GF, Farnsworth NR, Mesecar AD, Bolton JL. 2005. Xanthohumol isolated from Humilus lupulus inhibits menadioneinduced DNA damage through induction of quinone reductase. Chemical Research in Toxicology 18:1296-1305   DOI   ScienceOn
23 Cha NY, Kim HJ, Chung CH, Cho YS. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J Korean Soc Food Sci Nutr 28:1310-1315
24 Do JC, Chai JY, Son JK. 1991. Studied on the consitituents of Lycopus lucidus(II). Kor J Pharmacogn 22:166-170
25 Goudard M, Bonvin JF, Lebreton P, Chopin J. 1978. Differentiation des dihydroxy-5,7 methoxy-6 ou 8 flavones, flavonols et mehyl-3 flavonols par spectrometrie de masse. Phytochemistry 17:145-146   DOI   ScienceOn
26 우원식. 1997. 천연물화학 연구법. pp.1-60. 서울대학교출판부