• The Korean Journal of Ecology
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• 제19권6호
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• pp.507-517
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• 1996

To explain the horizontal expansion of a rhizomatous perennial, Convallaria keiskei(lily-of-the-valley), in a study site of Chunchon, Kangwon Province, Korea, ramet growth and clonal structure were studied. Remarkable growth stategies were clarified. First, the timing for the successive phenological events such as sprouting. flowering and rhizome growth for lily-of-the-valley was fitted to exploit early spring when the canopy of overstory was opened. Second, these events were supported by effective matter allocation pattern: for example, two-year investment for new rhizomes enabled the first year ramets to mature in six weeks after sprouting and to grow up to 85% of the leaf area of perennial ramets. Finally, the ramet population was increased by local disturbances such as freezing, herbivory and collection by human. The rule that a clone was supposed to produce one new thizome per year was broken by occasional disturbances. Then, up to 5rhizomes from latent bur could be redeveloped. Based on clonal structure, 80% or total clones have from 1 to 4 ramets. this means there have occurred minor disturbances. Therefore, in conclusion, the successful flourishing of lily-of-the-valley came from its effective frowth strategy to take advantage of site disturbance.

• 원예과학기술지
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• 제32권4호
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• pp.544-549
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• 2014

Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) are the most prevalent viruses infecting lilies in Korea. Leaf and bulb samples showing characteristic symptoms of virus infection were collected in 2012, and 80 field samples were analyzed by reverse transcription polymerase chain reaction (RT-PCR). The infection frequencies were 79% for LMoV, 5% for LSV, and 3% for CMV. The LMoV coat protein gene was amplified and cloned into the pET21d(+) expression vector to develop serological diagnostic tools to detect LMoV. The resulting carboxy-terminal His-tagged coat proteins were expressed in Escherichia coli strain BL21 (DE3) by induction with IPTG. The recombinant proteins were purified using Ni-NTA agarose beads and used as an antigen to produce polyclonal antibodies in laying hens. The resulting egg yolk immunoglobulin (IgY) specifically recognized LMoV from infected plant tissues in immunoblotting assays and had comparable sensitivity to that of a mammalian antibody. In addition, method of immunocapture RT-PCR using this IgY was developed for sensitive, efficient, and rapid detection of LMoV. Based on these results, large-scale bulb tests and detection of LMoV in epidemiological studies can be performed routinely using this IgY. This is the first report of production of a polyclonal IgY against a plant virus and its use for diagnosis.

• The Plant Pathology Journal
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• 제23권4호
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• pp.300-307
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• 2007

Previously, a chloroplast-localized Cu,Zn superoxide dismutase (chCu,ZnSOD) was isolated from lily and the sense- and antisense- sequences of the lily chCu,ZnSOD were used to transform potato plants. Two selected lines, the sense- and anti-sense strand of transgenic plants, were further characterized for resistance to Erwinia carotovora, which is a severe pathogen affecting potato plants. Only the sense-strand transgenic potato, which contained less $O_2^{.-}$ and more $H_2O_2$ than wild-type and antisense-strand transgenic plants, showed increased resistance to E. carotovora. Additional studies using $O_2^{.-}$ or $H_2O_2$ scavengers in wild-type, sense-strand, and antisense-strand transgenic plants suggest that resistance to E. carotovora is induced by reduced $O_2^{.-}$ and is not influenced by $H_2O_2$. To the best of our knowledge, this report is the first study suggesting that resistance to E. carotovora is enhanced by reduced $O_2^{.-}$, and not by increased amounts of $H_2O_2$.

• KSBB Journal
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• 제17권6호
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• pp.577-581
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• 2002

풍부한 식물의 화분을 이용하여 재조합단백질의 생산연구를 위하여 UreB 단백질 정보를 지닌 1.7 kb DNA를 Helicobacter pylori urease gene cluster를 지니는 pH808로보터 PCR을 통하여 증폭하고 이를 CaMV35S promoter에 연결하여 백합(Lilium longiflorum)화분내로 도입하고 기내배양을 실시하였다. 발아초기의 화분을 Agrobacterium과 함께 진공침윤시켜 ureB DNA를 형질전환시키고 kanamycin을 지니는 화분배지에서 16-24시간 배앵하여 완전한 화분관신장을 이루도록 하였다. 이들 형질전환화분의 유전자도입 및 발현을 분석하였으며 그 결과 기내배양하는 하분을 일회성의 단백질공장으로 이용할 수 있다는 가능성을 제시하였다.

• 농업과학연구
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• 제25권2호
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• pp.321-328
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• 1998

This study was performed to provide reference materials for the vegetable cultivating farmhouses by investigating profit and marketing situation for the folk vegetables. The survey areas covered 10 cities and counties including Kumsan, 72 farmhouses, and 8 crops including buffterbur and wild rocambole. As a result, the cultivated area of folk vegetables was increased as much as 13.7%, however, marketing cost was higher than that of horticultural crops, because they were not accessed to wholesale market. It was that the optimal period to sell the folk vegetables was shown like Jan. - Feb. for butterbur and day lily, Nov. - Dec. for wild rocambole, and Oct. - Nov. for kind of lettuce respectively. As the result of analyzing economical efficiency, the net profit per 0.1 ha was amounted to 3,438,000 won for butterbur, 3.291,000 won for wild rocambole 1,712,000 won for day lily, and 1,464,000 won for fatsia shoots. Besides, these four crops occupied more than 60% in cultivation area, and their profit was high. Therefore, it is necessary to foster them with special crops in Chnugnam area. The crops that require more labor time were such crops as 632.9 hours for day lily, 584.3 hours for butterbur, and 409.2 hours for fatsia shoots, whereas the crops that required less labor time were such field crops as 219.2 hours for fragrant edible wild aster and 233.8 hours for kind of lettuce.

• 한국자원식물학회지
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• 제33권6호
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• pp.689-695
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• 2020

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'Milky way'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• 원예과학기술지
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• 제29권1호
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• pp.74-76
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• 2011

An interspecific hybrid lily cultivar 'Green Star' was bred in 2005 at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Korea. The crossing and in vitro embryo rescue was conducted between Lilium FA97-2 (L. ${\times}$ formolongi 'Silky White' ${\times}$ L. Asiatic 'Sunray') and L. Asiatic 'Bomi (Byeongga ${\times}$ Connecticut King)' by cut style pollination method (CSM) at Suwon in 2000. The first selection was done and was tentatively named as 'FA03-5' in 2003. After in vitro multiplication and bulbing production of 'FA03-5' line, growth and flowering characteristic tests were conducted from 2003 to 2005. The evaluation of characteristics and consumer preferences were surveyed at a lily flower show of NIHHS in 2005. 'Green Star' flowered in the middle of June and grew more than 120 cm stem in length. Flowers bloomed facing upward, unspotted in petals and greenish yellow (RHS, Y6D). 'Green Star' was male sterile. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. It was needed to control the Botrytis disease in wet season.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.35-35
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• 2021

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'MilkyWay'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 ㎕ PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• 농업과학연구
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• 제43권5호
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• pp.734-741
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• 2016

Lily (Lilium davidii) is a high-yielding flowering plant. Besides roses and chrysanthemums, lily bulbs have long been used as food and in oriental medicine. However, the usage and value of cut lily bulbs has not been recognized. A bulb whose yield has been decreased is called a waste bulb, and a large amount of such bulbs is discarded every year. In this study, the functionality of waste bulbs from cut lilies was investigated to explore their potential use as a value-added product. We divided lily bulbs into two groups, one group with six varieties of new bulbs (Medusa, Siberia, Woori Tower, Yelloween, Le Reve, and Morning Star) used for cultivation and the other group with six varieties of waste bulbs (Medusa, Siberia, Woori Tower, Yelloween, Sorbonne, and Sheila). Physiological activities (${\alpha},{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl: DPPH) and 3-ethlbenzthiazolne-6-sulfonic acid (ABTS) radical scavenging capability and tyrosinase inhibiting activity), the amount of total as well as eight individual phenolic compounds (chlorogenic acid, epicatechin, rutin hydrate, p-coumaric acid, kaempferol 3-O-${\beta}$-rutinoside, phloridzin dihydrate, myricetin, and quercetin), and total flavonoid content were measured in the bulbs by high performance liquid chromatography. We detected high amounts of total phenol and total flavonoid as well as high DPPH and ABTS radical scavenging ability. More tyrosinase inhibiting activity was detected in the new bulbs than in the waste bulbs. However, both the new and waste bulbs showed a higher inhibitory activity than the standard (100 ppm ascorbic acid). Although the content of phenolic compounds differed among varieties, under the conditions of the experiment, the most abundant phenolics were epicatechins, followed by chlorogenic acid, and rutins. Overall, the waste bulbs had a higher content of these compounds than the new bulbs. Based on these results, we concluded that bulbs from cut lilies could be used as functional foods in the future and farmers could expect economic gain from the hitherto neglected waste bulbs.

• 식물병연구
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• 제10권4호
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• pp.319-323
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• 2004

본 연구는 부생성 균류인 Ulocladium atrum을 이용하여 Botrytis spp.에 의한 백합 잎마름병 및 오이 잿빛곰팡이병의 방제효과를 조사하고 그 작용특성을 규명함으로서 U. atrum의 생물학적 방제제로서의 이용 가능성을 평가하기 위해 실시하였다. 토마토 잎의 표면과 조직 속에서 분리한 Ulocladium sp. CNU 9037과 CNU 9054 균주는 형태적 특징에 의하여 U. atrum로 동정되었으며 이것이 U. atrum의 국내 최초의 보고이다. 토마토 및 오이의 고사된 잎을 이용하여 U. atrum의 Botrytis spp. 포자형성억제력을 실내검정으로 조사하였던 바, U. atrum은 고사된 잎 절편에서 Botrytis의 포자형성을 효과적으로 억제하였다. Botrytis의 포자형성억제력은 U. atrum을 Botrytis보다 먼저 처리했을 경우 효과가 높았다. 백합 잎마름병 방제를 위한 포장(온실)실험 결과 U. atrum CNU 9037 균주의 포자현탁액 처리는 백합 잎마름병을 효과적으로 억제하였으며 방제가는 70%, 77%로 procymidone 약제 처리구의 방제가(58%)보다 우수하였다. U. atrum(CNU 9037, CNU 9054) 포자현탁액처리는 오이 잿빛곰팡이병도 효과적으로 억제하였으며, 그 방제가는 66${\sim}$71%였다. 본 연구의 결과는 U. atrum 균주가 오이, 백합의 Botrytis병의 생물학적 방제제로서 개발 가능성이 있음을 나타낸다