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Analysis of UreB Protein Synthesis from Transgenic Lily Pollen  

박희성 (대구가톨릭대학교 생명자원학부)
박인혜 (대구가톨릭대학교 생명자원학부)
Publication Information
KSBB Journal / v.17, no.6, 2002 , pp. 577-581 More about this Journal
Abstract
In an attempt to produce recombinant proteins using the pollen enriched in some plant species, a 1.7 kb DNA encoding urease subunit B (UreB) amplified by PCR from Helicobacter pylori urease gene cluster in pH808 plasmid was cloned to be expressed under CaMV35S promoter in lily (Lilium longiflorum) pollen tubes elongated in vitro. Lily pollen at early germinating stage was transformed with the ureB DNA using Agrobacterium via vacuum infiltration and, incubated for a full pollen tube growth 16 - 24 h in the dark in the presence of kanamycin. DNA integration and expression in the transgenic pollen were analyzed by the standard molecular techniques and the results suggest that the pollen in vitro may be employed as a protein factory in a disposable fashion.
Keywords
Pollen; tansformation; vacuum infiltration; urease;
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