• 한국자원식물학회지
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• 제14권1호
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• pp.24-31
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• 2001

천궁에서는 butylidene phthalide, butyl phthalide, senkyunolide, cnidilide + neocnidilide 및 ligustilide가 동정되었으며, 이들 성분의 70% 이상을 ligustilide와 senkyunolide가 차지하였다. Phthalide류 총량으로 볼 때 연길산 및 안국산의 중국재배 천궁은 한국재배종보다 약간 높은 경향이었으나, 길림산은 한국 재배종보다 뒤떨어지는 것으로 나타났으며 각 성분의 함량도 낮아 품질이 나쁜 것으로 확인되었다. 또한, 중국의 연길산 천궁은 ligustilide와 senkyunolide의 광분해생성물인 butylidene phthalide 및 butyl phthalide함량이 높아 유통기간과 보관상의 문제가 있었다고 판단되어져 약재 수입시 유의하여야 할 것으로 사료된다. 당귀에서는 butylidene phthalide와 ligustilide가 동정 되었으며, 전체의 약 90%를 ligustilide가 차지하였다. 우리나라 재배종 당귀 중의 ligustilide 함량은 중국 연길산의 상품 및 하품보다 높았으나, 안국산 및 길림산보다는 낮았다. 그러나 길림산을 제외한 중국산 당귀는 ligustilide의 광분해산물로 생성되는 butylidene phthalide함량이 우리 나라 재배종에 비하여 훨씬 높은 것으로 나타나, 천궁에서와 같이 중국산 약재의 유통과 보관상의 문제점이 제기되었다.

• 한국자원식물학회지
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• 제5권1호
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• pp.57-68
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• 1992

The effects of various kind and amount of nitrogen fertilizers on the plant grorth. physiological , yield and extracts. ligustilide, butylidene phthalide contents of curdsdrug "Tou-ki" (AnTelica acutiloba Kitagawa) were investigated in 1989. Five plots variouskind of nitrogen fertilizers. Namely, no nitrogen, urea. ammonium nitrate, ammoniumchloride and control plot of ammonium sulfate. The other, by providing five plotsdifferent composed ratio of nitrogenous fertilizers. containing no nitrogen (No. o) .0.5-fold nitrogen (No s), control plot of 1.0-fold nitrogen(Nl.o). 1.5-fold nitrogen(Nl s)and 2.0-fold nitrogen Na. o), but nitogen was used the ammonium sulfate. The results showedthat the crude drug "Tou-ki" can ammonium chloride be produced in good yield, displayingboth good plant growth and remarkable physiologically-active conditions, and it can beproduced such that the extracts is maximized. Additionally, using methods of gaschromatography (GC) , it was established that ligustilide and butylidene phthalide, majorcomponents in the crude drug was recovered in a good yield from the fully grown plants.The other, the plant growth, the physiologically-active, the weight of whole plant, theyield of extracts and ligustilide, butylidene phthalide were seen to be best at the plotof 2.0-fold nitrogen and according to the increase or decrease of nitrogen decreasedgradually. Therefore. about 2 fold of standard quantity seems to be the most suitablequantity of nitrogen for "Tou-ki" cultivation .uot;Tou-ki" cultivation .ion .

• 한국식품위생안전성학회지
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• 제32권3호
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• pp.193-198
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• 2017

본 연구는 건강기능식품 중 baicalin, eleutheroside E, ligustilide를 효과적으로 분석할 수 있는 방법을 확립하기 위하여 수행되었다. 이에 LC-MS/MS를 이용하여 신속하고 효율적으로 동시분석할 수 있는 시험법을 확립하였으며, 확립된 시험법에 대해 특이성, 검출한계, 정량한계, 정확도, 정밀도에 대한 검증을 통하여 유효성을 확인하고자 하였다. 표준용액을 이용하여 검량선을 작성한 결과 $r^2$ > 0.99 이상의 직선성을 확인하였고, baicalin, eleutheroside E, ligustilide에 대한 정량한계는 각각 $39.3{\mu}g/L$, $106.7{\mu}g/L$, $76.1{\mu}g/L$이었으며, 검출한계는 각각 $13.0{\mu}g/L$, $35.2{\mu}g/L$, $25.1{\mu}g/L$이었다. 또한 평균 회수율은 각 성분에 대해 108.0~109.9%, 99.8~101.3%, 91.4~97.2%로 나타났으며, 반복정밀도는 상대표준편차 5%이하, 실험실간 재현성은 9%이하로 나타나 정확성, 재현성이 우수하였으며 이는 AOAC 가이드라인에서 제시한 기준에 모두 적합한 수준이었다. 따라서 개발된 분석법은 향후 건강기능식품 중 baicalin, eleutheroside E, ligustilide를 동시분석하는데 효과적으로 활용할 수 있을 것으로 판단된다.

• Natural Product Sciences
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• 제16권4호
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• pp.259-264
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• 2010

The essential oil fraction was obtained from the underground parts o of Ligusticum chuanxiong (Umbelliferae) by steam distillation, and its main components, Z-ligustilide and butylidene phthalide, were isolated by column chromatography. Its essential oil fraction and the isolated main components were examined for effects on their anti-inflammatory properties in RAW 264.7 macrophage cells to develop a new natural anti-inflammatory drug. The results showed that the L. chuanxiong essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the production of nitric oxide significantly in lipopolysaccharide (LPS)-treated RAW 264.7 cells. LPS-induced interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-$\alpha$) production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that the L. chuanxiong essential oil fraction and also its main components, Z-ligustilide, and butylidene phthalide reduced the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).

• 한국약용작물학회지
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• 제16권3호
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• pp.168-172
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• 2008

The root of Angelica tenuissima Nakai (Umbelliferae) has been used in traditional medicines of Korea as a headache, common cold and a fever remedy. A. tenuissima contains ferulic acid and various compounds of essential oil group such as limonene, 3-butylidenephthalide, ${\gamma}$-terpinene, neocnidilide, ligustilide, senkyunolide and neocnidilide. This study carried out to compare the contents of ferulic acid, z-ligustilide and n-butylidenephthalide between native and cultivated species of A. tenuissima by HPLC. The average contents of ferulic acid, z-ligustilide and n-butylidenephthalide indicated that native species (9 samples) were 0.060%, 0.616%, 0.025% and cultivated species (15 samples) were 0.037%, 0.141%, 0.029%, respectively. All samples were collected from different places in Korea.

• 생약학회지
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• 제21권1호
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• pp.69-73
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• 1990

Cnidium Rhizome(Chuan-Xing) used as sedatives, the treatment of anemia, woman's disease and ozena etc. has been one of the important oriental medicines. This paper deals with a comparison of butylidene phthalide and ligustilide as main components between rhizome of Conioselinum sp. and rhizome of Cnidium officinale by HPLC. The contents of butylidene phthalide in rhizome of Conioselinum sp. and rhizome of Cnidium officinale were found to be 0.083%, 0.067% and that of ligustilide to be 0.304%, 0.272% respectively.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1061-1067
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• 2018

The anti-melanogenic effects of the extract of Angelica tenuissima (AT) root and the extract of AT root fermented by Aspergillus oryzae (FAT) were investigated. These effects were determined by measuring the inhibitory activity of AT and FAT on melanin production in B16F10 melanocytes and with in vitro tyrosinase activity assays. The AT extract inhibited melanin production at concentrations above $250{\mu}g/ml$, and this inhibitory effect was significantly enhanced by the fermentation process with A. oryzae. HPLC analysis resulted in the isolation of two active compounds from both the AT and FAT extracts. Their chemical structures were identified as decursin and Z-ligustilide through comparison with previously reported NMR data. The decursin and Z-ligustilide contents were increased in the FAT extract and could be responsible for its enhanced inhibitory effects on melanin production and tyrosinase activity compared with that of the AT extract.

• 대한한의학방제학회지
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• 제19권2호
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• pp.93-108
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• 2011

Objectives : Angelica sinensis has been used extensively in Korean traditional medicine. Although thorough clinical trials are lacking, Various pharmacological actions for Angelica sinensis has been identified newly using animal models. In addition, it was reported that reactive intermediates, potentially causing toxic effects, was isolated from components in Angelica sinensis. In this article, it was purposed for explanation and introduction of new studies for Angelica sinensis. in terms of Pharmacological action and Toxicity. Methods : New studies for Angelica sinensis were reviewed and summarized in terms of pharmacological action and toxicity. Results and Conclusions : Angelica sinensis and its components including phthalide, phthalide dimers, polysaccharides, polyacetylenes, ligustilide와 butylidenephthalide as well as organic acid showed a variety of pharmacological actions on uterine, cardiovascular system and immune system. In addition, it was identified that three components of such as ligustilide, caffeic acid and safrole was biotransformed to reactive intermediates causing possibly toxicity.

• 한국식품과학회지
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• 제29권2호
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• pp.189-193
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• 1997

Angelicae tenuissimae Radix의 정유성분에 대한 구조규명과 채취장소에 따른 정유성분의 정량실험 결과 및 Ligustici rhizoma와의 정성 정량적 차이점에 관한 실험을 하여 다음과 같은 결론을 얻었다. Ligustici rhizoma와 Angelicae tenuissimae Radix 산지별 네가지 시료의 용출함량을 비교한 결과 경북 석포에서 채집한 시료가 11.32%로 가장 높은 값을 나타내었고, 중국산 Ligustici rhizoma가 3.49%로 가장 낮은 용출함량을 나타냈다. GC/MS로 구조를 분석한 결과 Angelicae tenuissimae Radix의 정유성 분은 ${\alpha}-pinene,\;{\alpha}-phellendrene,\;{\delta}-3-carene,\;{\gamma}-terpinene$, 4-vinylguaiacol, ${\alpha}-farnesene,\;(t)-{\beta}-farnessene,\;{\gamma}-elemene$, sesquiterpene alcohol, butylphthalide, senkyunolide, butylidenephthalide, z-butylidenedihydrophthalide (z-ligustilide) 등을 확인할 수 있었는데, Ligustici rhizoma 정유성분에서는 Angelicae tenuissimae Radix와 다르게 senkyunolide가 38.7%로 주성분이었으며, butylphthalide(17%), cnidilide(8.8%), z-ligustilide(8.8%) 등을 함유하였다. 따라서 Ligustici rhizoma와 Angelicae tenuissimae Radix의 주성분이 서로 상이하며, 그 조성비도 큰 차이가 있음을 확인할 수 있었다. 본 연구 결과로부터 고본의 주성분은 ligustilide (한국산 고본)과 senkyunolide (중국산)로서 앞으로 임상실험을 통하여 이들 성분의 정확한 약리효과를 규정함으로써 고본(藁本)을 규격화 하여야 할 것으로 생각된다.

• 생약학회지
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• 제21권2호
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• pp.121-125
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• 1990

Essential oil of the root of Angelica dahurica Benth et Hook(Umbelliferae) was investigated. Essential oil was obtained from the dried roots by steam distillation and fractionated by column chromatography. Each isolate or fraction was identified by GC, GC-MS and spectral analysis. It was found to contain eleven monoterpenes such as ${\alpha}-pinene(4.74%),\;campben, {\beta}-pinene,\;myrcene,\;{\alpha}-phellandrene,\;{\delta}-3-carene(39.4%),\;{\alpha}-terpinene,\;{\rho}-cymene,\;{\beta}-phellandrene,\;{\alpha}-terpinene,\;terpinolene\;and\;also\;found\;to\;contain\;4-vinylguaiacol,\;iso-elemicin,\;{\beta}-elemene$, caryophyllene, ligustilide, osthol and seven tentatively identified sesquiterpenes.