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Decursin and Z-Ligustilide in Angelica tenuissima Root Extract Fermented by Aspergillus oryzae Display Anti-Pigment Activity in Melanoma Cells

  • Park, Yuna (College of Health Science, Kangwon National University) ;
  • Kim, Dayoung (Department of Chemistry and Nano Science, Global Top 5 Program, Ewha Womans University) ;
  • Yang, Inho (Department of Convergence Study on the Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Choi, Bomee (Department of Chemistry and Nano Science, Global Top 5 Program, Ewha Womans University) ;
  • Lee, Jin Woo (College of Health Science, Kangwon National University) ;
  • Namkoong, Seung (College of Health Science, Kangwon National University) ;
  • Koo, Hyun Jung (Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries) ;
  • Lee, Sung Ryul (Department of Convergence Biomedical Science, Cardiovascular and Metabolic Disease Center, College of Medicine, Inje University) ;
  • Park, Myung Rye (Korea Research Institute Bio Science Co., Ltd.) ;
  • Lim, Hyosun (Korea Research Institute Bio Science Co., Ltd.) ;
  • Kim, Youn Kyu (Korea Research Institute Bio Science Co., Ltd.) ;
  • Nam, Sang-Jip (Department of Chemistry and Nano Science, Global Top 5 Program, Ewha Womans University) ;
  • Sohn, Eun-Hwa (College of Health Science, Kangwon National University)
  • Received : 2018.02.26
  • Accepted : 2018.04.11
  • Published : 2018.07.28

Abstract

The anti-melanogenic effects of the extract of Angelica tenuissima (AT) root and the extract of AT root fermented by Aspergillus oryzae (FAT) were investigated. These effects were determined by measuring the inhibitory activity of AT and FAT on melanin production in B16F10 melanocytes and with in vitro tyrosinase activity assays. The AT extract inhibited melanin production at concentrations above $250{\mu}g/ml$, and this inhibitory effect was significantly enhanced by the fermentation process with A. oryzae. HPLC analysis resulted in the isolation of two active compounds from both the AT and FAT extracts. Their chemical structures were identified as decursin and Z-ligustilide through comparison with previously reported NMR data. The decursin and Z-ligustilide contents were increased in the FAT extract and could be responsible for its enhanced inhibitory effects on melanin production and tyrosinase activity compared with that of the AT extract.

Keywords

References

  1. Pham AK, D inulos JG. 2014. Cosmeceuticals for children: should you care? Curr. Opin. Pediatr. 26: 446-451. https://doi.org/10.1097/MOP.0000000000000105
  2. Desmedt B, Courselle P, De Beer JO, Rogiers V, Grosber M, Deconinck E, et al. 2016. Overview of skin whitening agents with an insight into the illegal cosmetic market in Europe. J. Eur. Acad. Dermatol. Venereol. 30: 943-950. https://doi.org/10.1111/jdv.13595
  3. Han BH, Sung CK, Takeatsu K, But PPH, Guo J-X. 1998. International Collation of Traditional and Folk Medicine. World Scientific Publishing Co. Pte. Ltd., Singapore.
  4. Weeratunga P, Uddin MB, Kim MS, Lee BH, Kim TH, Yoon JE, et al. 2016. Interferon-mediated antiviral activities of Angelica tenuissima Nakai and its active components. J. Microbiol. 54: 57-70. https://doi.org/10.1007/s12275-016-5555-4
  5. Ahn SJ, Baek JM, Cheon YH, Park SH, Lee MS, Oh J, et al. 2015. The inhibitory effect of Angelica tenuissima water extract on receptor activator of nuclear factor-kappa-B ligand-induced osteoclast differentiation and bone resorbing activity of mature osteoclasts. Am. J. Chin. Med. 43: 715-729. https://doi.org/10.1142/S0192415X15500445
  6. Lee SH, Choi H, Kim H, Lee H, Sung YH, Kim SE, et al. 2010. Inhibitory effect of Angelicae Tenuissimae Radix on expressions of cyclooxygenase-2 and inducible nitric oxide synthase in mouse BV2 microglial cells. Neurol. Res. 32 Suppl 1: 58-63. https://doi.org/10.1179/016164109X12537002794048
  7. Desmedt B, Van Hoeck E, Rogiers V, Courselle P, De Beer JO, De Paepe K, et al. 2014. Characterization of suspected illegal skin whitening cosmetics. J. Pharm. Biomed. Anal. 90: 85-91. https://doi.org/10.1016/j.jpba.2013.11.024
  8. Shin YK, Son HU, Kim JM, Heo JC, Lee SH, Kim JG. 2015. Cinnamomum cassia bark produced by solid-state fermentation with Phellinus baumii has the potential to alleviate atopic dermatitis-related symptoms. Int. J. Mol. Med. 35: 187-194. https://doi.org/10.3892/ijmm.2014.2006
  9. Kim OK, Chang JY, Nam DE, Park YK, Jun W, Lee J. 2015. Effect of Canavalia gladiata extract fermented with Aspergillus oryzae on the development of atopic dermatitis in NC/Nga mice. Int. Arch. Allergy Immunol. 168: 79-89. https://doi.org/10.1159/000441654
  10. Frias J, Song YS, Martinez-Villaluenga C, Gonzalez de Mejia E, Vidal-Valverde C. 2008. Immunoreactivity and amino acid content of fermented soybean products. J. Agric. Food Chem. 56: 99-105. https://doi.org/10.1021/jf072177j
  11. Oh SW, Park S-H, Lee HS, Kang M, Lee SE, Yoo JA, et al. 2017. Melanogenic mechanism of ethanolic extract of Dalbergia odorifera. Mol. Cell. Toxicol. 13: 453-459. https://doi.org/10.1007/s13273-017-0049-y
  12. Gillbro JM, Olsson MJ. 2011. The melanogenesis and mechanisms of skin-lightening agents - existing and new approaches. Int. J. Cosmet. Sci. 33: 210-221. https://doi.org/10.1111/j.1468-2494.2010.00616.x
  13. Videira IF, Moura DF, Magina S. 2013. Mechanisms regulating melanogenesis. An. Bras. Dermatol. 88: 76-83. https://doi.org/10.1590/S0365-05962013000100009
  14. Maeda K, Fukuda M. 1996. Arbutin: mechanism of its depigmenting action in human melanocyte culture. J. Pharmacol. Exp. Ther. 276: 765-769.
  15. Smit N, Vicanova J, Pavel S. 2009. The hunt for natural skin whitening agents. Int. J. Mol. Sci. 10: 5326-5349. https://doi.org/10.3390/ijms10125326
  16. Briganti S, Camera E, Picardo M. 2003. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res. 16: 101-110. https://doi.org/10.1034/j.1600-0749.2003.00029.x
  17. DeCaprio AP. 1999. The toxicology of hydroquinone - relevance to occupational and environmental exposure. Crit. Rev. Toxicol. 29: 283-330. https://doi.org/10.1080/10408449991349221
  18. Atanasov AG, Waltenberger B, Pferschy-Wenzig EM, Linder T, Wawrosch C, Uhrin P, et al. 2015. Discovery and resupply of pharmacologically active plant-derived natural products: a review. Biotechnol. Adv. 33: 1582-1614. https://doi.org/10.1016/j.biotechadv.2015.08.001
  19. Kim BS, Seo H, Kim HJ, Bae SM, Son HN, Lee YJ, et al. 2015. Decursin from Angelica gigas Nakai inhibits B16F10 melanoma growth through induction of apoptosis. J. Med. Food 18: 1121-1127. https://doi.org/10.1089/jmf.2014.3397

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