• Journal of Microbiology and Biotechnology
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• 제29권9호
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• pp.1453-1459
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• 2019

Zeaxanthin is an important pigment in the photo-protection mechanism of microalgae. However, zeaxanthin epoxidase, an enzyme involved in the accumulation and conversion of zeaxanthin, has not been extensively studied in microalgae. In this work, we report the expression pattern of zeaxanthin epoxidase in Dunaliella tertiolecta (DtZEP) at different light and diverse salinity conditions. To confirm the responsiveness to light conditions, the ZEP expression pattern was investigated in photoperiodic (16 h of light and 8 h of dark) and continuous (24 h of light and 0 h of dark) light conditions. mRNA expression levels in photoperiodic conditions fluctuated along with the light/dark cycle, whereas those in continuous light remained unchanged. In varying salinity conditions, the highest mRNA and protein levels were detected in cells cultured in 1.5 M NaCl, and ZEP expression levels in cells shifted from 0.6 M NaCl to 1.5 M NaCl increased gradually. These results show that mRNA expression of DtZEP responds rapidly to the light/dark cycle or increased salinity, whereas changes in protein synthesis do not occur within a short period. Taken together, we show that DtZEP gene expression responds rapidly to light irradiation and hyperosmotic stress. In addition, ZEP expression patterns in light or salinity conditions are similar to those of higher plants, even though the habitat of D. tertiolecta is different.

• ALGAE
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• 제22권4호
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• pp.319-323
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• 2007

This study was conducted with using chlorophyll a fluorescence (indicated as photosynthetic activity) to examine the toxic effect of 96 h exposure of heavy metals and herbicides on the benthic diatom Nitzschia sp. which was isolated from pristine sediment in Pamquat Harbour, Nova Scotia, Canada. Samples of benthic diatom were exposed to 0, 0.01, 0.1 and 1 mg L–1 of copper, 0, 1, 10 and 100 mg L–1 of chrome (VI), 0, 2.45, 24.5 and 245 mg L–1 of paraquat dichloride, and 0, 4.37, 43.7 and 437 mg L–1 of alachlor during 96 hours. The effective quantum yield of photochemistry (ΔF/Fm’) was evaluated by subjecting light acclimated samples to saturating pulses of light using a pulse amplitude modulated (PAM) fluorometer. The impact of heavy metals on Nitzschia sp. photosynthesis was not severe in < 1 mg L–1 but in the high concentrations (> 1 mg L–1) clearly increased toxic stress during 96 h. Herbicides had a limited impact during the exposure period but clearly increased stress on the benthic diatom with increasing concentrations. Acute response of Nitzschia sp. to selected heavy metals and herbicides was characterized, and the capacity of a benthic diatom to tolerate and recover from toxic stress was assessed.

• 한국정밀공학회지
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• 제25권9호
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• pp.38-44
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• 2008

Light guide plate is one of most important components which are composed of back light unit, affecting the quality and performance of LCD. Average brightness and uniformity are especially key factors for designing the light guide unit. These qualities are affected and controlled by the pattern being attached to the back of light guide unit. In order to obtain high brightness and uniformity the optimized pattern design is adopted for LGP. In this study, optimized molding condition for LGP with 0.4 mm thickness was obtained by using the Moldflow simulation software and the optimized pattern for better brightness uniformity was designed for the thickness of the 0.4 mm by trial and error method. The brightness was measured for the different LGP thicknesses and the residual stress analysis was performed for 0.4 mmthickness by the photoelasticity and the results are compared with 0.5 mm, 0.6 mm thickness.

• 한국농공학회논문집
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• 제46권2호
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• pp.93-103
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• 2004

In trench excavation, essential factor of earth-retaining temporary work structure should be easy taking to pieces and movement, and dead weight must be less. This paper studies about the light weight material and application as earth-retaining structure to prevent the slope failure of sand soil ground caused by the variation of groundwater level in trench excavation. That is, light weight earth-retaining structural is proposed and a simulation with FEM on application of proposed structural in sandy soil is presented. The results are summarized as follows; (1) The study proposed FRP H-shaped pannel for the light weight member, and also presented estimation method about stability. (2) Mechanical property (bending moment, shear force, axial force, displacement) were changed according to groundwater level, but these values had been within enough safety rate and allowable stress. Therefore, proposed light weight pannel with FRP is available for bracing structure in trench excavation.

• 한국작물학회지
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• 제24권1호
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• pp.57-65
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• 1979

대.소맥에 대한 유리 Proline 축적의 온도범위, 장기저온처리의 경향, 저온과 수분부족에 있어서 광의재생의 여부 및 수종의 식물에 대한 종간 및 종내의 변이 등 일련의 반응생리와 축적기작에 대한 실험결과를 요약하면 다음과 같다. 1. 대맥은 8$^{\circ}C$나 그 이하의 온도에서 축적되나 소맥은 12$^{\circ}C$나 그 이하의 온도에서 Proline을 축적하였다. 2. 대.소맥에 있어서 신장억제온도와 Proline을 축적하기 시작하는 온도는 거의 일치하였다. 3. 엽절편을 5$^{\circ}C$의 저온에 둔 것은 29일 생존하였으며 그동안 Proline을 축적하였으나 2$0^{\circ}C$에 둔 것은 13일 밖에 생존하지 못하였으며 그 동안 축적은 없었다. 4. 완전식물체엽과 엽절편 공히 5$^{\circ}C$의 저온에 서의 축적은 광의재생이였다. 5. 광의재생은 Proline의 생합성을 위한 전구물질의 급여로는 대체될 수 없었다. 6. 수분부족에 의한 Proline 축적은 2$0^{\circ}C$에서는 광의재생이 아니였으나 5$^{\circ}C$에서는 광의재생이였다. 7. 저온과 수분부족에 대한 종간 및 종내의 축적변이는 높은 편이였다. 그러나 수분부족이 저온에 대한 변이보다 높았다. 8. 저온과 수분부족에 의한 Proline 축적의 기작은 상당히 상이한 것 같다.

• 한국생산제조학회지
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• 제4권4호
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• pp.50-60
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• 1995

Recently advantages joining dissimiliar materials and light weight material techniques have led to increasing use of structural adhesives in the various industries. Stress singulartiy occurs at the interface edges of adhesively bonded dissimilar materials. So it is required to analyze its stress singularity at the interface edges of adhesively bonded joints indissimilar materials. In this paper, the analysis method of stress singularity is studied in detail. Also, effects of the stress singularity at the interface edge of adhesively bonded scarf joints in combinations of dissimilar materials are investigated by using 2-dimensional elastic program of boundary element method. As the results, the strength evaluation method of adhesively bonded dissimilar materials using the stress singularity factor, $\Gamma$,is very useful. The fracture criterion, method of strength evaluation and prediction of fracture strength by the stress singularity factor on the adhesively bonded dissimilar materials are proposed.

• 한국건설순환자원학회논문집
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• 제2권4호
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• pp.321-327
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• 2014

경량골재콘크리트는 구조물의 자중경감을 목적으로 경량골재를 적용하여 제조한 콘크리트로 구조물의 고층화 및 대형화에 효과적으로 대응할 수 있는 장점이 있다. 하지만 경량골재는 다공체이므로 화재와 같은 고온에 노출되는 경우 수증기압이나 열응력에 의해 골재 주변으로 응력이 집중될 가능성이 커 폭렬이 발생하기 쉽다. 본 연구는 구조용 경량골재를 사용한 1종 경량골재콘크리트의 함수율과 내화특성과의 관계를 검토코자 하며, 실험결과 압축강도와 함수율이 높은 경량골재콘크리트는 폭렬의 가능성이 높기 때문에 흡수율이 작은 골재의 사용이나 폭렬방지를 위한 대책이 필요하다.

• Journal of Animal Science and Technology
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• 제64권5호
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• pp.985-996
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• 2022

This study investigated the effects of light intensity on growth performance, blood components, eye condition, and carcass characteristics of broiler chickens. Three hundred and fifty-two 1-day-old male chicks were assigned to one of four treatments (four repetitions per treatment, 22 birds per repetition) and reared in a floor pen for 5 weeks. From the second week, chicks were reared under four different levels of light intensity (5, 20, 35, and 50 lx) and the lighting duration was maintained at 18-hours light : 6-hours dark (18L : 6D). The feed intake and body weight were measured weekly. At 35 days of age, 12 birds per treatment were randomly selected for blood sampling, eye measurement, and carcass analysis. There were no significant differences in body weight gain, feed intake, and feed conversion ratio among treatments. Triglyceride levels in the serum were significantly higher in the 5 lx treatment, and creatinine was significantly lower in the 5 lx treatment (p < 0.05). The heterophil : lymphocyte ratios decreased significantly as light intensity increased (p < 0.05); however, other blood cell compositions were not affected by light intensity. Interleukin-6 content was significantly higher in the 5 lx treatment than in other treatments (p < 0.05), but the content of tumor necrosis factor-α was not significantly different among treatments. Serum corticosterone concentration was significantly higher at 5 lx than at 20, 35, and 50 lx (p < 0.05). The corneal diameter was the highest in 5 lx treatment (p < 0.05), and tended to increase as the light intensity decreased. Other eye parameters were not significantly different among treatments, but displayed a tendency to increase as the light intensity decreased. Carcass yield and part yields were not affected by light intensity. Meat quality parameters (pH, color, cooking loss, and water-holding capacity) did not show significant difference among the treatments. The results indicate that a light intensity of 5 lx may increase physiological stress or have a negative effect on broiler welfare, even if the performance and carcass characteristics are not affected. Therefore, a light intensity of 20 lx or above is recommended considering both the growth performance and welfare of broilers.

• Molecules and Cells
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• 제23권2호
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• pp.154-160
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• 2007

An Arabidopsis hy4 mutant that is specifically impaired in its ability to undergo blue light dependent photomorphogenesis was used to identify cryptochrome 1 signaling-related components. Proteomic analysis revealed about 205 differentially expressed protein spots in the blue light-irradiated hy4 mutant compared to the wild-type. The proteins corresponding to 28 up-regulated and 33 down-regulated spots were identified. Obvious morphological changes in the hy4 mutant were closely related to the expression of various transcription factors. Our findings suggest that blue light signals may be involved in many cellular processes including disease resistance and stress responses.

• 생명과학회지
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• 제25권5호
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• pp.585-593
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• 2015

Stress fiber (SF) 변화는 세포외부의 결합인자와 세포 수용체와 결합후 리모델링을 위해 액틴골격에 신호를 전달하며 일어난다. 이 연관은 결합장소에서 기계적 활동과 신호전달활동을 조절하는 다양한 스케폴드들과 신호 전달자에 의해 매게된다. Heterotrimeric transmembrane lymphotoxin α1β2 (LTα1β2)는 용해성 homotrimeric LT α를 포함하는 tumor necrosis factor (TNF) 계로 림프조직을 구성하는데 중요한 역할을 한다. LTα1β2와 LTβR의 결합은 fibroblastic reticular cell (FRC)에서 신호전달을 촉발한다. Agonistic anti-LTβR antibody 단독 혹은 LTα 그리고 TNFα의 조합으로 LTβR 자극은 세포의 액틴과 형태적 변화를 보았다. Agonistic anti-LTβR antibody의 FRC에서 작용을 통한 세포골격 재배열이 myosin과의 관련성을 확인하기위해 myosin light chain kinase (MLCK)의 저해제인 ML-7과 myosin light chains (MLC)와 myosin phosphatase target subunit 1 (MYPT1)의 인산화에 대한 효과를 확인하였다. MLCK 저해는 액틴 세포골격 재배열과 세포형태 변화를 유도하였다. 또한, MLC와 MYPT1인산화가 LTβR 자극에 의해 줄어드는 것을 확인하였다. DNA chip 분석은 myosin and actin 구성선분이 전사체 수준에서도 줄어드는 것을 보였다. 결론적으로 LTβR 자극은 FRC에서 SF변화는 myosin과 관련되어 있다는 것을 제시한다.