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Comparative Proteomic Analysis of Blue Light Signaling Components in the Arabidopsis Cryptochrome 1 Mutant  

Phee, Bong-Kwan (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Park, Sebyul (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Cho, Jin-Hwan (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Jeon, Jong-Seong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Bhoo, Seong Hee (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Hahn, Tae-Ryong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
Publication Information
Molecules and Cells / v.23, no.2, 2007 , pp. 154-160 More about this Journal
Abstract
An Arabidopsis hy4 mutant that is specifically impaired in its ability to undergo blue light dependent photomorphogenesis was used to identify cryptochrome 1 signaling-related components. Proteomic analysis revealed about 205 differentially expressed protein spots in the blue light-irradiated hy4 mutant compared to the wild-type. The proteins corresponding to 28 up-regulated and 33 down-regulated spots were identified. Obvious morphological changes in the hy4 mutant were closely related to the expression of various transcription factors. Our findings suggest that blue light signals may be involved in many cellular processes including disease resistance and stress responses.
Keywords
Arabidopsis; Blue Light; Cryptochrome 1; hy4; Hypocotyl Elongation; Proteomic Analysis;
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Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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1 Blum, H., Beier, H., and Hans, J. G. (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93−99
2 Chen, M., Chory, J., and Fankhauser, C. (2004) Light signal transduction in higher plants. Annu. Rev. Genet. 38, 87−117
3 Folta, K. M., Pontin, M. A., Karlin-Neumann, G., Bottini, R., and Spalding, E. P. (2003) Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light. Plant J. 36, 203−214
4 Hudson, M. E., Lisch, D. R., and Quail, P. H. (2003) The FHY3 and FAR1 genes encode transposase-related proteins involved in regulation of gene expression by the phytochrome Asignaling pathway. Plant J. 34, 453−471
5 Park, K. and Kang, H. M. (2004) Cloning and circadian expression of rat Cry1. Mol. Cells 18, 256−260
6 Rabilloud, T., Adessi, C., Giraudel, A., and Lunardi, J. (1997) Improvement of the solubilization of proteins in twodimensional electrophoresis with immobilized pH gradients. Electrophoresis 18, 307−316
7 Raval, S., Gowda, S. B., Singh, D. D., and Chandra, N. R. (2004) A database analysis of jacalin-like lectins: sequence-structurefunction relationships. Glycobiology 14, 1247−1263
8 Lin, C., Ahmad, M., Gordon, D., and Cashmore, A. R. (1995b) Expression of an Arabidopsis cryptochrome gene in transgenic tobacco results in hypersensitivity to blue, UV-A, and green light. Proc. Natl. Acad. Sci. USA 92, 8423−8427
9 von Arnim, A. and Deng, X. W. (1996) Light control of seedling development. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 215−243
10 Phee, B.-K. and Bhoo, S. H. (2003) Proteomic analysis of light stress response in Arabidopsis thaliana. Agric. Chem. Biotechnol. 46, 47−51
11 Lin, C. (2002) Blue light receptors and signal transcription. Plant Cell 14 (suppl.), S207−S225
12 Rabilloud, T. (1998) Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis. Electrophoresis 19, 758−760
13 Jiao, Y., Yang, H., Ma, L., Sun, N., Yu, H., et al. (2003) A genome- wide analysis of blue-light regulation of Arabidopsis transcription factor gene expression during seedling development. Plant Physiol. 133, 1480–1493
14 Phee, B.-K., Cho, J.-H., Park, S., Jung, J. H., Lee, Y.-H., et al. (2004) Proteomic analysis of the response of Arabidopsis chloroplast proteins to high light stress. Proteomics 4, 3560−3568
15 Ohgishi, M., Saji, K., Okada, K., and Sakai, T. (2004) Functional analysis of each blue light receptor, cry1, cry2, phot1, and phot2, by using combinatorial multiple mutants in Arabidopsis. Proc. Natl. Acad. Sci. USA 101, 2223−2228
16 Christie, J. M. and Briggs, W. R. (2001) Blue light sensing in higher plants. J. Biol. Chem. 276, 11457−11460
17 Blum, D. E., Neff, M. M., and van Volkenburgh, E. (1994) Light-stimulated cotyledon expansion in the blu3 and hy4 mutants of Arabidopsis thaliana. Plant Physiol. 105, 1433− 1436
18 Ma, L., Li, J., Qu, L., Hager, J., Chen, Z., et al. (2001) Light control of Arabidopsis development entails coordinated regulation of genome expression and cellular pathways. Plant Cell 13, 2589−2607
19 Rabilloud, T., Valette, C., and Lawrence, J.-J. (1994) Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension. Electrophoresis 15, 1552−1558
20 Ahmad, M. and Cashmore, A. R. (1993) HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366, 162−166
21 Lin, C., Robertson, D. E., Ahmad, M., Raibekas, A. A., Shuman, J. M., et al. (1995a) Association of flavin adenine dinucleotide with the Arabidopsis blue light receptor CRYl. Science 269, 968−970   DOI
22 McNellis, T. W. and Deng, X. W. (1995) Light control of seedling morphogenetic pattern. Plant Cell 7, 1749−1761
23 Lin, C. and Shalitin, D. (2003) Cryptochrome structure and signal transduction. Annu. Rev. Plant Biol. 54, 469−496
24 Koornneef, M., Rolff, E., and Spruit, C. J. P. (1980) Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana (L.) Heynh. Z. Pflanzenphysiol. 100, 147−160
25 Hudson, M., Ringli, C., Boylan, M. T., and Quail, P. H. (1999) The FAR1 locus encodes a novel nuclear protein specific to phytochrome A signaling. Genes Dev. 13, 2017−2027
26 Cashmore, A. R., Jarillo, J. A., Wu, Y.-L., and Liu, D. (1999) Cryptochromes: Blue light receptors for plants and animals. Science 284, 760−765