Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Comparative Proteomic Analysis of Blue Light Signaling Components in the Arabidopsis Cryptochrome 1 Mutant

  • Phee, Bong-Kwan (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Park, Sebyul (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Cho, Jin-Hwan (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Jeon, Jong-Seong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Bhoo, Seong Hee (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Hahn, Tae-Ryong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
  • Received : 2006.09.14
  • Accepted : 2007.02.15
  • Published : 2007.04.30
⟨ Previous Next ⟩

Abstract

An Arabidopsis hy4 mutant that is specifically impaired in its ability to undergo blue light dependent photomorphogenesis was used to identify cryptochrome 1 signaling-related components. Proteomic analysis revealed about 205 differentially expressed protein spots in the blue light-irradiated hy4 mutant compared to the wild-type. The proteins corresponding to 28 up-regulated and 33 down-regulated spots were identified. Obvious morphological changes in the hy4 mutant were closely related to the expression of various transcription factors. Our findings suggest that blue light signals may be involved in many cellular processes including disease resistance and stress responses.

Keywords

Acknowledgement

Supported by : KOSEF, KRF

References

  1. Ahmad, M. and Cashmore, A. R. (1993) HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366, 162−166
  2. Blum, D. E., Neff, M. M., and van Volkenburgh, E. (1994) Light-stimulated cotyledon expansion in the blu3 and hy4 mutants of Arabidopsis thaliana. Plant Physiol. 105, 1433− 1436
  3. Blum, H., Beier, H., and Hans, J. G. (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93−99
  4. Cashmore, A. R., Jarillo, J. A., Wu, Y.-L., and Liu, D. (1999) Cryptochromes: Blue light receptors for plants and animals. Science 284, 760−765
  5. Chen, M., Chory, J., and Fankhauser, C. (2004) Light signal transduction in higher plants. Annu. Rev. Genet. 38, 87−117
  6. Christie, J. M. and Briggs, W. R. (2001) Blue light sensing in higher plants. J. Biol. Chem. 276, 11457−11460
  7. Jiao, Y., Yang, H., Ma, L., Sun, N., Yu, H., et al. (2003) A genome- wide analysis of blue-light regulation of Arabidopsis transcription factor gene expression during seedling development. Plant Physiol. 133, 1480–1493
  8. Folta, K. M., Pontin, M. A., Karlin-Neumann, G., Bottini, R., and Spalding, E. P. (2003) Genomic and physiological studies of early cryptochrome 1 action demonstrate roles for auxin and gibberellin in the control of hypocotyl growth by blue light. Plant J. 36, 203−214
  9. Hudson, M., Ringli, C., Boylan, M. T., and Quail, P. H. (1999) The FAR1 locus encodes a novel nuclear protein specific to phytochrome A signaling. Genes Dev. 13, 2017−2027
  10. Hudson, M. E., Lisch, D. R., and Quail, P. H. (2003) The FHY3 and FAR1 genes encode transposase-related proteins involved in regulation of gene expression by the phytochrome Asignaling pathway. Plant J. 34, 453−471
  11. Koornneef, M., Rolff, E., and Spruit, C. J. P. (1980) Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana (L.) Heynh. Z. Pflanzenphysiol. 100, 147−160
  12. Lin, C. (2002) Blue light receptors and signal transcription. Plant Cell 14 (suppl.), S207−S225
  13. Lin, C., Ahmad, M., Gordon, D., and Cashmore, A. R. (1995b) Expression of an Arabidopsis cryptochrome gene in transgenic tobacco results in hypersensitivity to blue, UV-A, and green light. Proc. Natl. Acad. Sci. USA 92, 8423−8427
  14. Lin, C., Robertson, D. E., Ahmad, M., Raibekas, A. A., Shuman, J. M., et al. (1995a) Association of flavin adenine dinucleotide with the Arabidopsis blue light receptor CRYl. Science 269, 968−970 https://doi.org/10.1126/science.7638621
  15. Lin, C. and Shalitin, D. (2003) Cryptochrome structure and signal transduction. Annu. Rev. Plant Biol. 54, 469−496
  16. Ma, L., Li, J., Qu, L., Hager, J., Chen, Z., et al. (2001) Light control of Arabidopsis development entails coordinated regulation of genome expression and cellular pathways. Plant Cell 13, 2589−2607
  17. McNellis, T. W. and Deng, X. W. (1995) Light control of seedling morphogenetic pattern. Plant Cell 7, 1749−1761
  18. Ohgishi, M., Saji, K., Okada, K., and Sakai, T. (2004) Functional analysis of each blue light receptor, cry1, cry2, phot1, and phot2, by using combinatorial multiple mutants in Arabidopsis. Proc. Natl. Acad. Sci. USA 101, 2223−2228
  19. Park, K. and Kang, H. M. (2004) Cloning and circadian expression of rat Cry1. Mol. Cells 18, 256−260
  20. Phee, B.-K. and Bhoo, S. H. (2003) Proteomic analysis of light stress response in Arabidopsis thaliana. Agric. Chem. Biotechnol. 46, 47−51
  21. Phee, B.-K., Cho, J.-H., Park, S., Jung, J. H., Lee, Y.-H., et al. (2004) Proteomic analysis of the response of Arabidopsis chloroplast proteins to high light stress. Proteomics 4, 3560−3568
  22. Rabilloud, T., Valette, C., and Lawrence, J.-J. (1994) Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension. Electrophoresis 15, 1552−1558
  23. Rabilloud, T., Adessi, C., Giraudel, A., and Lunardi, J. (1997) Improvement of the solubilization of proteins in twodimensional electrophoresis with immobilized pH gradients. Electrophoresis 18, 307−316
  24. Rabilloud, T. (1998) Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis. Electrophoresis 19, 758−760
  25. Raval, S., Gowda, S. B., Singh, D. D., and Chandra, N. R. (2004) A database analysis of jacalin-like lectins: sequence-structurefunction relationships. Glycobiology 14, 1247−1263
  26. von Arnim, A. and Deng, X. W. (1996) Light control of seedling development. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 215−243