• Title/Summary/Keyword: light curve solution

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Determination of Horseradish Peroxidase (HRP) using an Enhanced Chemiluminescence Assay (증강 화학발광 기법을 이용한 horseradish peroxidase(HRP)의 검량)

  • Kim, Wongee;Kim, Keunhan;Lee, Seungmok
    • Journal of Korean Society on Water Environment
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    • v.25 no.1
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    • pp.84-89
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    • 2009
  • Our nation's water resources remain susceptible to contamination by phenolic agrichemicals. These compounds can be toxic to a variety of organisms including humans. Their disposal is restricted in many countries with strict limits for acceptable concentrations in drinking water. Enzyme-mediated in situ stabilization has been advocated as an approach for the treatment of phenolic compounds in soils and groundwater. This study reports the development of a new approach to quantify the activity of the HRP enzyme in aqueous systems. The method is based on the coupled processes of energy transfer and enhanced chemiluminescence using a luminol-$H_2O_2$-HRP system. In this study, the effects of solution pH, ionic strength and aqueous concentrations of HRP, $H_2O_2$ and enhancer were evaluated on the p-iodophenol-enhanced, HRP-catalyzed chemiluminescence reaction intensity in Tris-HCl buffer. All assay components were found to affect the maximum chemiluminescene intensity. The calibration curve for HRP showed the linear relationship with maximum light intensity.

The First Comprehensive Photometric Study of the Neglected Binary System V345 Cassiopeiae

  • Jeong, Min-Ji;Kim, Chun-Hwey
    • Journal of Astronomy and Space Sciences
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    • v.30 no.4
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    • pp.213-221
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    • 2013
  • We present the first BVRI CCD photometric observations of V345 Cas made on 22 nights during the observing seasons in 2007-2008. Our light curves, resembling those of EB-type binary stars, are analyzed with the 2003 version of the Wilson-Devinney binary code. The photometric study shows that V345 Cas is a typical semi-detached binary system with the primary star being about ${\Delta}T$ = 2400 K hotter, two times more massive, but only 17% larger than the secondary star being filled with it's Roche-lobe. The orbit has a relatively large inclination of about $88^{\circ}$. A cool spot on the secondary component is modeled to explain a small light curve asymmetry. Absolute dimensions and related radiometric parameters of the eclipsing pair are calculated and their evolutionary states are discussed with the HR diagrams of mass-radius and temperature-luminosity. A period analysis of all available times of minima, including our measurements, indicates that the orbital period may vary in a cyclical way, unfortunately the secondary period for the variation can not be uniquely determined because of lack of present timing data.

Development of Chemiluminescence Immunoassay For the Measurement of Serum Thyroxine(T4)

  • Kim, J.B.;Choe, B.K.;Choi, S.H.
    • Korean Journal of Animal Reproduction
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    • v.11 no.1
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    • pp.16-21
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    • 1987
  • We describe a simple, solid-phase chemiluminescence immunoassay for the meausrement of serum T4. An immunoglobulin G fraction of antibody to thyroxine was passively absorbed onto the walls of polystyrene tubes. The labeled antigen was thyroxine-aminobutylethylisoluminol. After the bindings reaction (37$^{\circ}C$ for 1 hour), the solution is removed by aspiration and the antibody-bound fraction was washed once with buffer. Sodium hydroxide (5mol/1,200${mu}ell$) was added and the mixture incubated for 30 minutes at 6$0^{\circ}C$. Luminescence was initiated by oxidation of the label with micropeeroxidase-hydrogen peroxide and the signal of light emission was intergrated for 10 sec. The light yield was inversely proportional to the concentration of T4 in the standard or sample. An evaluation of the method gave the following values sensitivity of calibration curve 7.5$\pm$2.8 nmol/l (mean$\pm$SD). The intra-assay precision (CV%) was 8.9, 7.3 and 5.4. The inter-assay precision (CV%) was 10.2, 8.1 and 7.1. When seum samples were assayed for T4, the results obtained by solid-phase CIA and the conventional RIA agreed well(n=3.5, r=0.954).

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A new merging-zone flow injection system for the quantification of ferrous and ferric ions in aqueous solution and sludge of wastewater

  • Farhood, Ahmed Saleh;Taha, Dakhil Nassir
    • Analytical Science and Technology
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    • v.35 no.5
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    • pp.218-227
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    • 2022
  • A simple and fast throughput flow injection (FI) system with a merging-zone technique was designed to determine ferrous and ferric in an aqueous solution. The method is based on the direct reaction of ferrous with a Bathophenanthroline reagent (Bphen) in acidic media. The forming red complex absorbs light at 533 nm. All conditions of the flow injection system were investigated. The analytical curve of ferrous was linear in the range of 0.07 to 4 mg/L with an r2 value of 0.9968. The detection and quantification limits were 0.02 and 0.04 mg/L, respectively. The molar absorptivity and Sandell's sensitivity were 4.0577 × 106 L/mol cm and 25 × 10-5 ㎍/cm2, respectively. The homemade valve was low-cost with high repeatability (n = 7) at an RSD of 1.26 % and zero dead volume. The values of the dispersion coefficient were 2.318, 2.022, and 1.636 for the concentrations of 0.2, 1, and 3 mg/L, respectively. The analysis throughput of the designed flow injection unit was 57 sample per hour.

EFFECT OF COMPRESSIVE RESIDUAL STRESS FOR CORROSION PROPERTY OF SUP-9 STEEL USING AS SUSPENSION MATERIAL

  • Park, K.D.;Ki, W.T.;Shin, Y.J.;Ryu, H.J.
    • International Journal of Automotive Technology
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    • v.8 no.3
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    • pp.343-349
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    • 2007
  • One of the useful technologies for light-weightening of components required in the automobile and machine industry is to use high strength materials. To improve material properties, the carbonizing treatment, nitrifying treatment, and shot-peening method are representatively applied. However, the shot-peening method is generally used to remove surface defect on steel and to improve the fatigue strength of the surface. Benefits of shot-peening are increasing resistance against fatigue, stress corrosion cracking, fretting, galling, erosion and closing of pores. In this paper, the effect of shot-peening on the corrosion of SUP-9 steel immersed in a 3.5% NaCl solution and corrosion characteristics from heat treatment during the shot peening process has been investigated. The immersion test was performed on four kinds of specimens. Corrosion potential, the polarization curve, residual stress etc. Were analyzed from the experimental results.

PHOTOMETRIC OBSERVATIONS AND ANALYSIS OF THE W UMa TYPE ECLOPSING BINARY VW Cep (W UMa형 식쌍성 VW Cep의 측광관측과 분석)

  • 강봉석;이용삼;정장해
    • Journal of Astronomy and Space Sciences
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    • v.17 no.1
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    • pp.19-32
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    • 2000
  • A total of 1,018 observations (509 in B, 509 in V) of the eclipsing binary VW Cep was made during 7 nights from April through May in 1999 at Sobaeksan Optical Astronomy Observatory, using the CCD camera attached to the 61cm telescope. A time of minimum light of HJD2451327.2282 was determined from our data, and we constructed BV light curves with the data. Using, Wilson-Devinney’s binary model, we analized the light curves. The absolute dimension of $M_1=0.95M_\odot,M_2=0.33M_\odot,R_1=1.02R_\odot,R_2=0.66R_\odot$ of the VW Cep system were derived from our light curve solution and Kaszas et al. (1998) spectroscopic results.

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DENTINAL MICROLEAKAGE STUDY ON THE LIGHT CURABLE RESTORATIVE GLASS IONOMER CEMENT (광중합형 충전용 글라스 아이오노머 시멘트의 상아질 변연누출에 관한 정량분석)

  • Shin, Dong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.20 no.2
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    • pp.832-838
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    • 1995
  • The purpose of this study was to evaluate the amount of marginal microleakage of 2 light curable GI cements(Fuji II LC & VariGlass), which contain some resin components. 4 volunteers kept on acrylic resin plates, which contained dentin disks with cavities filled with test materials for 2 weeks. The time when polishing was done(5 minutes and 24 hours after filling) and the use of protective agents were varied, so 8 groups with each 6 specimens were tested. After having specimens(disks with cavities filled with materials) penetrated with 1% Methylene Blue solution, specimens were stored in 40% nitric acid solution for 4 days to extract adsorbed dye material. Supernatants of centrifuged samples were diluted 5 times and Spectrophotometer was used to determine the degree of absorption. Dye concentration was calculated through the pre-obtained Linear Regression Curve. The results were as follows. 1. The best result was seen in groups (PF24, PV24) which were protected and polished 24 hours later and the opposite phenomenon was seen in groups(NF24, NV24) which were held without protection and polished 24 hours later. Groups polished S minutes later showed moderate leakage pattern. 2. Groups polished 5 minutes later showed similar leakage amount irrespective of using of protective agent. But statistically insignificant lower values were seen in VariGlass than in Fuji II LC groups, So It was considered that VariGlass may be more resistant to early moisture attack than Fuji II LC. 3. In groups polished 24 hours later, there was no significant difference between materials but was definitely significant difference according to the use of protective agent. If the cement in which polishing will be done 24 hours later, Protective agent should be used to cover the surface.

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Preparation Method of Indigo Standard Solution and Variation of Indigo Contents in Blue Dye Extract from Breeding Lines of Persicaria tinctoria H. Gross (인디고 분석 표준액의 제조법 및 쪽 육성계통의 인디고 함량변이)

  • Kim, Seong Ju;Ko, Jae Hyung;Park, Si Hyung;Kim, Myeong Seok;Kim, Kwan Su
    • Korean Journal of Medicinal Crop Science
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    • v.21 no.3
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    • pp.213-219
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    • 2013
  • This study was carried out to find out the optimum method of preparation of indigo standard solution and its stability, and to investigate the indigo contents in Niram, blue dye extract, from a total of 7 indigo plants and 34 breeding lines of Persicaria tinctoria H. Gross. Proper solvent for indigo standard was dimethyl sulfoxide (DMSO), and appropriate concentration was 1 mg of indigo in 10 mL of DMSO. Absorbance value of UV/Vis Spectrophotometer at 620 nm of standard solution was changed decreasingly 12 hours after the preparation of standard solution irrespective of the storage conditions such as temperature and light. Average value of absorbance of 8-fold diluted standard solutions prepared daily during 16 days was $0.210{\pm}0.005$, indicating the powder of indigo compound was stable chemically. Calibration curve was made for quantitative analysis of indigo of 7 Niram samples, and indigo contents ranged from 0.69% to 18.76% showing relatively larger variation. Across all 34 breeding lines, the range of indigo content was from 7.9 mg to 56.4 mg per 100 g of fresh leaves, averaging 25.2 mg of indigo content and showing a 47.7% coefficient of variation.

Effect of Corrosion Atmosphere and Strain Rate on the Stress Corrosion Cracking of High Strength 7xxx Aluminum Alloy (고강도 7xxx 알루미늄 합금의 응력부식균열에 미치는 부식환경과 응력속도의 영향)

  • Yun, Yeo-Wan;Kim, Sang-Ha
    • Journal of the Korean institute of surface engineering
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    • v.41 no.3
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    • pp.121-128
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    • 2008
  • High strength 7xxx aluminum alloys have been applied to automotive bump back beam of the some limited model for light weight vehicle. The aluminum bump back beam is manufactured through extrusion, bending and welding. The residual stress given on these processes combines with the corrosive atmosphere on the road spreaded with corrosive chemicals to melt snow to occur the stress corrosion cracking. The composition of commercial 7xxx aluminum has Zn/Mg ratio about 3 and Cu over 2 wt% for better strength and stress corrosion cracking resistivity. But this composition isn't adequate for appling to the automotive bump back beam with high resistance to extrusion and bad weldability. In this study the composition of 7xxx aluminum alloy was modified to high Zn/Mg ratio and low Cu content for better extrusion and weldability. To estimate the resistivity against stress corrosion cracking of this aluminum alloy by slow strain rate test, the corrosion atmosphere and strain rate separate the stress corrosion cracking from conventional corrosion must be investigated. Using 0.6 Mol NaCl solution on slow strain rate test the stress corrosion cracking induced fracture was not observed. By adding 0.3% $H_2O_2$ and 0.6M $Na_2SO_4$ to 1M NaCl solution, the corrosion potential and current density of polarization curve moved to active potential and larger current density, and on the slow strain rate test the fracture energy in solution was lower than that in pre-exposure. These mean the stress corrosion cracking induced fracture can be estimated in this 1M NaCl + 0.3% $H_2O_2$ + 0.6M $Na_2SO_4$ solution. When the strain rate was below $2{\times}10^{-6}$, the stress corrosion cracking induced fracture start to be observed.

PHOTOELECTRIC OBSERVATIONS AND UBVRI LIGHT CURVES ANALYSIS OF ALGOL (Algol의 광전측광관측과 UBVRI 광도곡선의 분석)

  • 정장해;이용삼;임조령;양감징
    • Journal of Astronomy and Space Sciences
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    • v.10 no.2
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    • pp.123-145
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    • 1993
  • UBVRi photometry of Algol was carried out from December of 1988 to March of 1991 at Chungbuk National University Observatory and a total of 3465 observations in U, B, V, R, I were obtained. Three times of primary minimum light of JDH el 2447898.0938, JEH el 2448265.1205 and JDH el 2448288.0598, and two secondary minimum light of JDH el 2447808.1014 and JDH el 2448275.146 were determined from our observations. We analyzed simultaneously the UBVRI light curves of the Algol system with the Wilson-Devinney method for the determination of the photometric parameters. Indivisual masses for the 3 components of Algol are derived as $m_1$=3.36, $m_2$=0.76, $m_3$=1.6 in solar mass and radii as $R_1$=2.97, $R_2$=0.76 in solar radinus using i=$82.{\circ}47$, q=0.227, $r_1$=0.2102, $r_2$=0.2512 of our solution and some parameters of the spectroscopic solution of Hill et al. (1971). Our results is simiar to those reported by Kim (1989). The temperature of Algol C, $T_3$=8800 was obtained by means of fitting $l_1$, $l_2$, and $l_3$ of five colors to Planckian curve, and $R_3$=$1.6R_\odot$ is derived from its result. It is believed that its semidetached configuration of Algol A and B is the consequence of case B mass transfer. According to its location in a mass-radius diagram. Algol b may have evolved significantly in its Hburnning phase.

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