• Molecules and Cells
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• 제38권10호
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• pp.918-924
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• 2015

During T cell activation, mitochondrial content increases to meet the high energy demand of rapid cell proliferation. With this increase, the level of reactive oxygen species (ROS) also increases and causes the rapid apoptotic death of activated cells, thereby facilitating T cell homeostasis. Nicotinamide (NAM) has previously been shown to enhance mitochondria quality and extend the replicative life span of human fibroblasts. In this study, we examined the effect of NAM on $CD8^+$ T cell activation. NAM treatment attenuated the increase of mitochondrial content and ROS in T cells activated by CD3/CD28 antibodies. This was accompanied by an accelerated and higher-level clonal expansion resulting from attenuated apoptotic death but not increased division of the activated cells. Attenuation of ROS-triggered pro-apoptotic events and upregulation of Bcl-2 expression appeared to be involved. Although cells activated in the presence of NAM exhibited compromised cytokine gene expression, our results suggest a means to augment the size of T cell expansion during activation without consuming their limited replicative potential.

• 한국공간구조학회논문집
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• 제23권3호
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• pp.105-113
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• 2023

The study used the whole-life carbon assessment method to conduct a thorough carbon-neutral evaluation of a standard steel structure. To further assess carbon emissions, 11 design-changed models were evaluated, with changes made to the span between beams and columns. The results of the carbon emission assessment showed savings of approximately 13.1% by implementing the stage of the beyond life cycle. Additionally, the evaluation of carbon emissions through design changes revealed a difference of up to 42.2%. These findings confirmed that recycling and structural design changes can significantly reduce carbon emissions by up to 48.6%, making it an effective means of achieving carbon neutrality. It is therefore necessary to apply the stage of beyond life cycle and structural change to reduce carbon emissions.

• 대한예방한의학회지
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• 제5권1호
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• pp.103-115
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• 2001

Background : Gamisamryungbaekchul-san(加味蔘?白朮散) is a herbal medicine which has been used for the traditional therapeutic agent of augmentation of the spleen and reinforcement of the Qi. Objective : This Study was performed to investigate the effect of Gamisamryungbaekchul-san on the tumor and immune response in the moose B16 melanoma tumor model. Materials and Methods : The tumor was induced by subcutaneous inoculation of B16BL6 melanoma cells in the shaved dorsal region of mice. Mice were orally administered with Gamisamryungbaekchul-san extract(26.3mg/mouse) for 14days after inoculation. For making examination of antitumor effect, the Increase of life span, Tumor growth inhibition rate, change of body weight were measured and evaluated. For the immune response increasing effect, the percentage of T lymphocyte and B Lymphocyte in the peripheral blood, the percentage of CD4+ T-cell, CD8+ T-cell and CD4+/CD8+ ratio in the peripheral blood and spleen, interleukin-2 productivity were measured and evaluated. Results : Gamisamryungbaekchul-san showed 16.59% increase of life span, 31.64% tumor growth inhibition rate and increase of body weight. Gamisamryungbaekchul-san increased the percentage of T lymphocyte in the peripheral blood, CD4+ T cell percentage of peripheral blood and spleen, and Interleukin-2 productivity as compared with the Control group. Whereas Gamisamryungbaekchul-san had no effect on the percentage of B lymphocyte in the peripheral blood, the percentage of CD8+ T cell, CD4+/CD8+ T-cell ratio in both of peripheral blood and spleen as compared with the Control group. Conclusion : This study shows that Gamisamryungbaekchul-san has anti-tumor effects and immunoregulatory effects on the B16 melanoma tumor model. It is suggested that Gamisarmyungbaekchul-san could be a useful immunomodulator and anti-tumor agent.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.258-261
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• 2006

The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When $20{\mu}g/{\mu}L$ of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most dearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.

• 생약학회지
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• 제33권3호통권130호
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• pp.224-229
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• 2002

Sarcodon aspratus (Thelephoraceae), a native mushroom distributed in Korea and Japan has been widely used as a traditional food and folk medicines. Two proteoglycan polysaccharides, named SAP-A and SAP-B were isolated from the fruitbody of S. aspratus. Their molecular weight were determined as 6184.9 D, and 25749.2 D, respectively, by maldi-tof ms. The composition of sugar and amino acid of these compounds was also determined. The SAP-A showed potent antitumor activity. The $ID_{50}$ value of SAP-A was 8.2 mg/kg/day for ICR mice transplanted with solid sarcoma-180, and the elongation of lifespan effect was 167.4% for ICR mice transplanted with ascitic sarcoma-180. Moreover, the elongation life-span effect of SAP-A increased dose-dependently.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1997년도 가을 학술발표회 논문집
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• pp.95-102
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• 1997

Jointless bridge is a new construction method applicable to bridge of short length. In the jointless bridge expansion of superstructure due to thermal effect was absorbed in the flexible pile-type abutment in stead of expansion joint in the conventional bridges. By removing expansion joint, it retards deterioration and extends life time of bridge. In this paper, jointless bridge of steel box girder type was studied through finite element analysis. Stress variations of superstructure and pile due to thermal effect was studied for the two span continuous integral bridge of 80m length and the results of analysis was presented.

• 생약학회지
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• 제11권1호
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• pp.7-10
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• 1980

Antitumor activity test was achieved with 20 crude drugs which are folkmedicine and recorded in Dong-eu-bo-gam. Three crude drugs showed above 60% effect on life span of mouse and 5 crude drugs showed under 50% effect. After death, body weight was decreased with 3 crude drugs and increased with most other crude drugs, which were tested in this study. The solid cancer tissue weight was decreased slightly with 4 crude drugs.

• Nuclear Engineering and Technology
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• 제53권9호
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• pp.3112-3121
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• 2021

One of the major factors affecting the life span of a Reactor Pressure Vessel (RPV) is the Pressurised Thermal Shock (PTS). PTS is a thermo-mechanical load on the RPV wall due to steep temperature gradients and structural load created by internal pressure of the fluid within the RPV. Safe operating life of a nuclear power plant is ensured by carrying out fracture analysis of the RPV against thermal shock. Carrying out fracture tests on RPV/large scale components is not always feasible. Hence, studies on laboratory level specimens are necessary to validate and supplement the prototype results. This paper aims to study the fracture behaviour of standard Compact Tension [C(T)] specimens, made of RPV steel 20MnMoNi55, subjected to thermal shock through experimental and numerical investigations. Fracture tests have been carried out on the C(T) specimens subjected to thermal transient load and tensile load to quantify the effect of thermal shock. Crack resistance curves are obtained from the fracture tests as per ASTM E1820 and compared with those obtained numerically using XFEM and a good agreement was found. A quantitative study on the crack tip plastic zone, computed using cohesive segment approach, from the numerical analyses justified the experimental crack initiation toughness.

• 농업과학연구
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• 제28권2호
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• pp.99-107
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• 2001

온실재배에 있어서 가장 경제적인 환경조절방법은 자연환기에 의한 것이므로 온실의 자연환기 성능을 극대화하는 것은 매우 중요하다. 온실의 자연환기 성능을 극대화하기 위해서는 지붕환기창의 설치가 중요한데, 아치형 단동 온실의 경우에는 천창을 설치하기 어려운 구조로 되어 있어서 대부분의 농가가 측창만 설치하여 운영하고 있다. 본 연구에서는 아치형 단동 온실의 지붕에 환기창 설치를 장려하기 위한 일환으로 사례조사와 효과분석을 실시하였다. 아치형 단동 온실의 지붕환기구조 실태조사 결과 폭 5~8m의 소형 온실에서는 플라스틱으로 제작된 원형 및 굴뚝형 환기창을, 폭 12~18m의 대형온실에서는 철재 파이프를 덧붙인 양권취식을 많이 사용하고 있었다. 지붕환기창 설치 온실과 관행의 권취식 측창만 설치된 온실에 대한 대조 실험 결과 비록 적은 면적의 지붕환기창을 설치하였지만 온도하강 $1^{\circ}C$ 이상의 천창 환기 효과가 있었으며, 지붕환기를 실시함으로써 온도분포를 보다 균일하게 할 수 있는 것으로 나타났다. 지붕환기창을 설치함으로서 얻을 수 있는 온도상승 억제효과는 일사량과 풍속조건에 따라 뚜렷한 차이를 보이며, 풍속이 낮은 경우에 효과가 커지는 것을 확인할 수 있었다. 환기창의 개폐순서에 따른 전체적인 온도 하강의 차이는 없으나, 환기로 온도를 제어하는 경우에는 실내온도가 설정온도보다 높을 때는 천창을 열은 후 측창을 열고, 반대의 경우에는 측창을 닫은 후 천창을 닫는 것이 바람직한 것으로 판단되었다.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1980년도 학술대회지
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• pp.87-113
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• 1980

This experiment was carried out to evaluate the effects of Korean ginseng extract on carcinogenesis induced by various chemical carcinogens. Red ginseng extract was used for this study and was administered orally to the experimental animals. Carcinogens that were injected in subscapsular region of ICR newborn mice within 24 hours after birth were 9,10-dimethyl-1,2-benzan-thracene (DMBA), urethane, N-2-fluorenylacetamide(AAF), aflatoxin $B_1$ and tobacco smoke condensate. N -methyl-N -nitroso-N'-nitroguani-dine(MNNG) was injected subcutaneously at the back of wistar rats. Experimental animals were autopsied in immediately after being sacrificed. All major organs were examined grossly and weighted. After fixation histopathological preparations were made for microscopical study. Following results were obtained. In DMBA group sacrificed at the 26th week after the treatment with DMBA, the incidence of lung adenoma was $77\%$ and the average number of the tumor was 17. However, in DMBA combined with red ginseng group, the incidence was $78\%$ and the average number of lung adenoma was 14.1. This indicates that ginseng extract had no effect on the incidence of lung adenoma but decreased the average number of lung adenoma by $17\%.$ In DMBA group sacrificed at the 48th week after the injection of DMBA, the lung adenoma incidence was $88\%.$ The average diameter of the largest lung adenoma was 3.5 cm, the incidence of diffuse pulmonary infiltration was $18\%$ and the average lung weight of male experimental mice was $528.2{\pm}469.1\;gm.$ On the other hand, in DMBA combined with red ginseng group sacrificed at the 48th week, the incidence of lung adenoma was $96\%.$ The average diameter of the largest adenoma was 2.7 cm, the incidence of diffuse pulmonary infiltration was $7\%$ and the average lung weight of male mice was $418.0{\pm}520\;gm.$ These observations show that ginseng extract did not have any inhibitory effect on the incidence of lung adenoma but decreased the average diameter of the largest lung adenoma by $23\%,$ the incidence of duffuse pulmonary infiltration by $63\%$ and the average lung weight of male experimental mice by $21\%.$ From these results we have found that the prolonged administration with ginseng extract showed no inhibitory effect on the incidence of adenoma but it had the inhibitory effect on the proliferation of lung adenomas induced by DMBA. In urethane group sacrificed at the 28th week after the injection of urethane, the incidence of lung adenoma was $94\%$ and the average number of lung adenoma was 8.6. In urethane combined with red ginseng group, the. incidence of lung adenoma was $73\%$ and the average number of adenoma was 6.0. These results indicate that there were $22\%$ decrease of the lung adenoma incidence and $31\%$ decrease of the average number of adenoma in urethane combined with red ginseng group. And in urethane group sacrificed at the 50th week, the incidence of lung adenoma was $98\%$ and the incidence of diffuse pulmonary infiltration was $14\%$. In urethane combined with ginseng group the incidence of lung adenoma was $85\%$ and the incidence of diffuse pulmonary infiltration was $12\%$. Therefore the ginseng administration resulted in $15\%$ decrease of the lung adenoma incidence and $14\%$ decrease of the diffuse pulmonary infiltration incidence. From these results we knew that the prolonged administration with ginseng extract inhibited the incidence and also the proliferation of the lung adenoma induced by urethane. Lung adenoma and hepatoma were induced in the experimental mice sacrificed at the 68th week but not in the experimental mice sacrificed at the 28th week after the injection of AAF. In AAF group sacrificed at the 68th week after the injection of AAF the incidence of lung adenoma was $18\%$ and the incidence of hepatoma was $27\%$. And in AAF combined with ginseng group the lung adenoma incidence was $12\%$ and the hepatoma incidence was $37\%$. So the ginseng seemed to decrease the lung adenoma incidence by AAF, but we were unable to conclude the significant inhibitory effect of the ginseng extract on the incidence of lung adenoma by AAF because the above incidence of lung adenoma were similar to that of control group which was $11\%$. And these experimental data revealed that ginseng extract didn't have any inhibitory effect on the incidence of hepatoma induced by AAF. In aflatoxin $B_1$ group sacrificed at the 56th week, the incidence of lung adenoma was $24\%$ and hepatoma was $11\%$. However in aflatoxin $B_1$ combined with ginseng group the incidence of lung adenoma was $17\%$ and hepatoma was $3\%$ These results indicate that there were $29\%$ decrease of the lung adenoma incidence and $75\%$ decrease of the hepatoma incidence in aflatoxin $B_1$ combined with ginseng group. In tobacco smoke condensate experimental group sacrificed at 67th week, no tumors were induced except just a few lung adenoma. The lung adenoma incidence both in tobacco smoke condensate group and in tobacco smoke condensate combined with ginseng group was $8\%$. And this incidence rate was similar to that of control group. These results indicate that the injection of 320 ug tobacco smoke condensate per ICR newborn mouse was unable to induce lung adenoma in our experiments. In MNNG group sacrificed at the 27th week the tumor incidence was $38.5\%$ and in MNNG combined with ginseng extract group was $37\%$. In MNNG group for investigation of the life span of tumor bearing rats the tumor incidence was $93\%$ and the average life span of tumor bearing rats was 318 days. And in MNNG combined with ginseng extract group the tumor incidence was $96\%$ and the average life span was 337 days. Tumor induced by MNNG was almost sarcoma. This indicates that there was no inhibitory effect of ginseng extract on the tumor incidence, but the extract prolonged the average life span of tumor bearing rats by approximately 19 days.