• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.12
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• pp.3788-3796
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• 2009

The aim of the study is to look at the relationships among emotional dimensions, commitment to environment, consumer typology, and quality of life. For this study, 303 students completed the questionnaires and SPSS and Amos 4 were employed. The findings are: First, positive emotion increases commitment to environment. Second, commitment to environment encourages green consumer behaviors and quasi-green consumer behaviors while it reduces non-green consumer behaviors. Third, green consumer behaviors increase quality of life while quasi-green consumer behaviors and non-green consumer behaviors do not have any influence on quality of life. Some practical implications are included.

• Journal of Microbiology and Biotechnology
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• v.20 no.7
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• pp.1114-1120
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• 2010

A chitosan-degrading fungus, BSF114, was isolated from soil. The culture preparation showed strong chitosanolytic enzyme activity at an optimum pH of 4.0 and optimum temperature of $60^{\circ}C$ after 36-40 h fermentation. The rapid decrease in the viscosity of the chitosan solution early in the reaction suggested an endo-type cleavage of the polymeric chitosan chains. To identify the isolated fungus, molecular biological and morphological methods were used. The fungal internal transcribed spacer (ITS) region 1 was amplified, sequenced, and then compared with related sequences in the GenBank database using BLAST. The phylogenetic relationships were then analyzed, and the results showed that the fungus belongs to Aspergillus fumigatus. Morphological observations were also used to confirm the above conclusion. The chitooligosaccharides (COS) obtained through hydrolyzing the colloidal chitosan showed that A. fumigatus BSF114 is suitable for degrading chitosan and producing chitooligosaccharides on a large scale. High concentrations of the COS (1,000 and 500 ${\mu}g/ml$) significantly proliferated mice marrow cells.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.1
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• pp.147-153
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• 2011

The Pseudorabies (PR), also called Aujeszky's disease (AD), is an infectious viral disease caused by an alpha herpes virus and has domestic and wild pigs, as well as a wide range of domestic and wild animals, as the natural host. Pseudorabies virus (PRV) virions contain several envelope glycoproteins. Among them, gB, gC and gD are regarded as the major immunogenic proteins. We expressed these glycoproteins using the bacterial expression system and analyzed recombinant proteins. Expression of glycoproteins gC and gD were observed on SDS-PAGE or Western blot analysis, but gB was not. Optimal concentration of IPTG and inducing time were determined as 1.0 mM and 4 h, respectively, for the expression of both gC and gD in E. coli. A sodium dodecyl sulfate (SDS) was the most efficient detergent in solubilizing insoluble recombinant protein.

• Journal of the Korean Society of Food Culture
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• v.30 no.5
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• pp.621-628
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• 2015

The objective of the current study was to analyze the major factors affecting quality of life improvement for contract foodservice customers by identifying the contract foodservice environment consisting of the customer, foodservice management, and competitor. Qualitative research method was performed on foodservice customers and foodservice management using in-depth interviews. First, the customer environment was classified into three categories, including convenience of location, foodservice management environment into six categories, including comfort level of dining facility, and competitor environment into three categories, including service competition between foodservice providers. Second, quality of life was defined as the level of contentment felt by both the customer and foodservice management consuming the food provided. Third, both the customer and foodservice management perceived that the management environment of contract foodservice had a "medium" effect on quality of customer life. The findings of this study could be applicable for development of a contract foodservice business strategy through objective comparative analysis of the customer, foodservice management, and competitor environments.

• Mycobiology
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• v.45 no.3
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• pp.204-208
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• 2017

Nosema ceranae is an obligate intracellular fungal parasite that causes mortality in honey bees and enhances the susceptibility of honey bees to other pathogens. Efficient purification of Nosema spores from the midgut of infected honey bees is very important because Nosema is non-culturable and only seasonably available. To achieve a higher yield of spores from honey bees, in this study, we considered that the initial release of spores from the midgut tissues was the most critical step. The use of 2 mm beads along with enzymatic treatment with collagenase and trypsin enhanced the homogenization of tissues and the yield of released spores by approximately 2.95 times compared with the use of common 3 mm beads alone. The optimal time for the enzyme treatment was determined to be 1 hr as measured by the yield and viability of the spores. A one-step filtration using a filter paper with an $8-11{\mu}m$ pore size was sufficient for removing cell debris. This method may be useful to purify not only N. ceranae spores but also other Nosema spp. spores.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.2
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• pp.75-82
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• 2011

To elucidate the novelty of Hyphantria cunea nucleopolyhedrovirus (HcNPV) isolated in Korea, polyhedrin and inhibitor of apoptosis (iap) gene structures were determined and analyzed. The analysis of HcNPV polyhedrin showed a little difference with 97.6% at the nucleotide level but no difference at the amino acid level when compared with that of previously reported H. cunea NPV (HycuNPV). On the other hand, iap genes showed variable differences with 89.0-99.6% nucleotide and 90.0-99.6% amino acid sequence identities. Especially, the 5' and 3' non-coding flanking sequences of iap1 gene had lower degree of identity with those of HycuNPV. Although the phylogenetic analyses using polyhedrin and iap genes showed that HcNPV is closely related with HycuNPV, we could provide that HcNPV is a novel isolate having novel gene structures.

• Journal of IKEEE
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• v.24 no.4
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• pp.1180-1183
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• 2020

Recently, the daily life safety detection functionalities such as fall accident detection and burn danger detection are widely disseminated along with the development of IoT and smart home. These safety detection functionalities are mostly performed by artificial intelligence. However, simple accuracy measurement of the safety detection in laboratory environment is often far from practical performance in daily life environment. To mitigate this problem, this paper introduces two techniques, i.e. living lab and confusion matrix. Living lab is more than simple simulation of daily life environment, and it enables users to directly participate technology development and product design. Various performance measures induced from confusion matrix significantly help to evaluate the performance of artificial intelligence system for proper application purposes.

• Journal of Families and Better Life
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• v.28 no.5
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• pp.53-66
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• 2010

The purpose of this study is to investigate the influence of family-friendly life environment factors including local community's living environment satisfaction (safety, convenience, comfort, accessibility, exchange of neighbor satisfaction), family support facility satisfaction, family support program satisfaction and nurture and education environment satisfaction, and local community's living information accessibility on the effect of multiple roles of married men and women with children. Data were collected from 380 married men and women with children located Seoul, GyeongGi-Do, ChungCheongBuk-Do, and KyungSangNam-Do. The results show that, first of all, related to general trend of role satisfaction and role strain, the subjects have higher experience of role satisfaction than role strain. Secondly, the variables of social demography, such as monthly income, non-regular full-time working system, white collar occupation, number of children and level of education, and living environment variables, such as satisfaction of living environment stability and satisfaction of neighbor exchange in living environment were significantly related to role strain. Thirdly, social demography variables, such as average monthly earnings, gender (male), non-regular part-time working system, satisfaction of stability in living environment, and satisfaction of convenience in living environment were significantly related to role strain.

• Proceedings of the Korean Society of Crop Science Conference
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• 2003.10a
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• pp.58-59
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• 2003

• Proceedings of the Korea Water Resources Association Conference
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• 2005.09b
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• pp.1378-1378
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• 2005