Journal of Microbiology and Biotechnology

  • 제20권7호
  • /
  • Pages.1114-1120
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  • 2010
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Isolation, Characterization, and Application of Chitosan-Degrading Fungus from Soil

  • Wei, Xinlin (Institute of Food Engineering, College of Life and Environment Science, Shanghai Normal University) ;
  • Chen, Wei (Institute of Food Engineering, College of Life and Environment Science, Shanghai Normal University) ;
  • Xiao, Ming (Institute of Food Engineering, College of Life and Environment Science, Shanghai Normal University) ;
  • Xiao, Jianbo (Institute of Food Engineering, College of Life and Environment Science, Shanghai Normal University) ;
  • Wang, Yuanfeng (Institute of Food Engineering, College of Life and Environment Science, Shanghai Normal University)
  • 투고 : 2009.09.20
  • 심사 : 2010.03.02
  • 발행 : 2010.07.28
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초록

A chitosan-degrading fungus, BSF114, was isolated from soil. The culture preparation showed strong chitosanolytic enzyme activity at an optimum pH of 4.0 and optimum temperature of $60^{\circ}C$ after 36-40 h fermentation. The rapid decrease in the viscosity of the chitosan solution early in the reaction suggested an endo-type cleavage of the polymeric chitosan chains. To identify the isolated fungus, molecular biological and morphological methods were used. The fungal internal transcribed spacer (ITS) region 1 was amplified, sequenced, and then compared with related sequences in the GenBank database using BLAST. The phylogenetic relationships were then analyzed, and the results showed that the fungus belongs to Aspergillus fumigatus. Morphological observations were also used to confirm the above conclusion. The chitooligosaccharides (COS) obtained through hydrolyzing the colloidal chitosan showed that A. fumigatus BSF114 is suitable for degrading chitosan and producing chitooligosaccharides on a large scale. High concentrations of the COS (1,000 and 500 ${\mu}g/ml$) significantly proliferated mice marrow cells.

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참고문헌

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