• The Korean Journal of Physiology
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• 제10권1호
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• pp.7-14
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• 1976

The Present study was conducted to investigate the effects of Ginseng extract on the tension-area curve for stearic acid monolayer. At the same time, the effects of Ginseng extract on osmotic and mechanical fragility of human red cells and histamine release from rabbit leukocytes were studied, The results are summarized as follows. 1. The Ginseng alcohol extract was found to expand liquid expanded phase of stearic acid monolayer, thus it is speculated that this agent may be acting as a surface active substance. 2. Osmotic hemolysis was inhibited by the Ginseng alcohol extract and the same effect was also observed in the presence of Ginseng saponin. However, the Ginseng alcohol extract was found to decrease hematocrit ratio of the RBC suspension, therefore, the inhibition of the osmotic hemolysis by this agent may be secondary effect to the reduced cell volume. 3. The mechanical hemolysis was also inhibited by the Ginseng alcohol extract but the inhibition was independent of changes in hematocrit ratio. 4. Histamine release from rabbit leukocytes was significantly increased in vitro in the presence of the Ginseng alcohol extract.(p<0.05)

• 대한수의학회지
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• 제36권2호
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• pp.337-348
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• 1996

The proportion of leukocyte subpopulation in the host is of a great importance in understanding their functions and disease progress. Many methods have been developed to seperate leukocytes and to measure their activities. Characterization of immune cell subpopulations in Korean native cattle was performed using a set of monoclonal antibodies specific which are specific to bovine leukocyte differentiation antigen. Peripheral blood leukocytes from fifty Korean native and ten Holstein cattle were collected and analyzed for the investigation of leukocyte subpopulation by using monoclonal antibodies and flow cytometry. The result indicated that Korean native cattle have significantly higher proportion of leukocyte subpopulations expressing MHC class II molecules and BoCD4 than Holstein cattle.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.177-177
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• 1996

Taurine Chloramine (Tau-Cl) inhibits production of nitric oxide(NO) and tumor necrosis factor-alpha (TNF-${\alpha}$) in activated peritoneal macrophages, similar In that previously reported for activated RAW 264.7 cells. In addition, the effect of Tau-Cl and taurine on superoxide anion (O$\_$2/$\^$-/) Production in murine peritoneal exudate polymorphonuclear leukocytes (PMN) was examined. Tau-Cl inhibited O$\_$2/$\^$-/ production in a manner that was dose-dependent and reversible, Taurine also inhibited O$\_$2/$\^$-/ production by stimulated PMN, but at higher concentrations and to a lesser extent than Tau-Cl. The effects of taurine on O$\_$2/$\^$-/ production was attributed to the in vitro formation of Tau-Cl catalyzed by PMN associated halide-dependent myeloperoxidase. In contrast, production of NO and TNF-${\alpha}$ by activated peritoneal exudate macrophages was inhibited by Tau-Cl while taurine was without effect. These data lend support to the notion that Tau-Cl may participate ill the inflammatory responses by modulating production of inflammatory mediators.

• 대한물리치료과학회지
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• 제8권1호
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• pp.923-934
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• 2001

In order to investigate a pathogenesis of liver damage induced by skin burn, thermal injury was induced by scald burn on entirely dorsal surface in rats (total body surface area 30%) except for inhalated injury. At 5 and 24 h after scald bum, biochemical assay and morphological changes in skin tissue, serum and liver tissue were examined. The effects of bum injury on the levels of glutathione, lipid peroxide and on the activities of oxygen free radical generating and scavenging enzymes have been determined in association with observing of histologic and ultrastructural changes, measuring the protein concentration in plasma, and counting the number of intravascular polymorphonuclear leukocytes. The activity of xanthine oxidase, an enzyme of oxygen free radical generating system. was elevated (p<0.01) in serum, but not in skin and in liver tissue. Futhermore, thermal injury decreased not only the protein concentration in plasma but also the number of leukocytes, that indicates induction of edema formation with protein exudation and inflammation by neutrophil infiltration into the internal organs. These data suggest that acute dermal scald burn injury leads to liver damage, that is related to elevation of xanthine oxidase activity in serum. Xanthine oxidase may be a key role in the pathogenesis of liver damage induced by skin burn.

• Nuclear Engineering and Technology
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• 제33권3호
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• pp.255-260
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• 2001

In mammals, most of the follicles can not be ovulated, and instead, are degenerated throughout the entire reproductive period. However, the precise mechanism of follicle atresia is unknown. Three weeks old female mice (ICR strain) were ${\gamma}$-irradiated with a dose of LD$^{50}$ . Before irradiation (day 0) and at day 1, 2, and 3 after irradiation, the normal and atretic preantral and antral follicles of the left ovaries were morphologically observed. Atretic follicles at 2 days after irradiation had numerous cell debris, apoptotic cells and bodies, and polymorphonuclear leukocytes in the antral cavity. In severely atretic follicles, numerous polymorphonuclear leukocytes infiltrated into the follicle. The frequencies of atretic antral (58.0 $\pm$8.6) and preantral follicles (27.3$\pm$11.2) induced by ${\gamma}$-radiation increased to 94.0$\pm$3.4 and 86.9$\pm$7.6, respectively at 2 days after irradiation (p<0.05). The number of follicles with one or more neutrophils in the largest cross sections at 2 and 3 days after irradiation significantly increased (p<0.05). It can be concluded that ${\gamma}$-radiation triggers the recruitment of neutrophils into the follicles during degeneration. The ovarian follicles can make a role of informative biological end-point useful for disaster-prevention.

• 한국동물위생학회지
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• 제29권3호
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• pp.365-376
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• 2006

To identify immune response of leukocytes in peripheral blood of cattle vaccinated with an attenuated live Akabane virus vaccine, leukocytes were reacted with monoclonal antibodies which are specific to bovine lymphocyte surface antigens and assayed by the flow cytometry. Serum neutralizing (SN) test was used to measure antibody titers after vaccination, SN antibody was appeared to 7 days post-vaccination (PV) and 2-8 antibody titers were observed in 14 days PV. Proportion of $CD8^-$ MHC $class II^+$ expressing cells were rapidly increased at 3 days PV. $CD8^+$ MHC $class II^-$ cells were increased at 7 days PV. $CD4^+CD8^-,\;WC^+CD4^-,\;CD4^+CD8^+,\;WC1^-CD4^+, \;WC1^-CD8^+$, and $CD4^-CD8^+$ cells were highly increased at 3, 3, 7, 7, 14, 14 days PV, respectively.

• Journal of Microbiology
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• 제39권3호
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• pp.186-194
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• 2001

Scrub typhus, caused by Orientia tsutsugamushi infection, is clinically and histopathologically characterized by local as well as systemic inflammatory reactions, indicating that orientiae induce mechanisms that amplify the inflammatory response. To reveal underlying mechanisms of chemoattraction and activation of responding leukocytes, expression of chemokine and tumor necrosis factor alpha (TNF-$\alpha$) genes in murine peritoneal macrophages after infection with the obligate intracellular bacterium Ο.tsutsugamushi was investigated. The genes that were unregulated included macrophage inflammatory proteins l$\alpha$/$\beta$(MIP-l$\alpha$/$\beta$), MIP-2, monocyte chemoattractant protein 1(MCP-1), RANTES (regulated upon activation, normal T-cell expressed and secreted), gamma-interferon-inducible protein 10(IP-10) and TNF-$\alpha$. Peak expression of these chemokines and TNF-$\alpha$ was observed between 1 and 3 h after infection. These responses returned to or approached baseline preinfection levels 6 h after challenge. Semiquantitative reverse transcription (RT)-PCR analysis revealed dramatic Increases during infection in the steady-state levels of mRNA ceding for the inhibitory subunit of NF-kB (IkB$\alpha$), whose transcription is enhanced by binding of NF-kB within the IkB$\alpha$promoter region. Thus, Ο. tsutsugamushi appears to be a stung inducer of chemokines and TNF-$\alpha$ which may significantly contribute to inflammation and tissue damage observed in scrub typhus by attracting and activating phagocytic leukocytes.

• IMMUNE NETWORK
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• 제13권3호
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• pp.102-106
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• 2013

Emerging evidence suggests that gap formation and opening of the endothelial junctions during leukocyte extravasation is actively controlled to maintain the integrity of the vascular barrier. While the role for endothelial cells to this process has been well defined, it is not clear whether leukocytes are also actively contributing to endothelial barrier function. We have recently showed that extravasating leukocytes deposit microparticles on the subendothelium during the late stages of extravasation, which is LFA-1 dependent. Using multiphotonintravital microscopy (MP-IVM) of mouse cremaster muscle vessels in the current work, we show that microparticle formation and deposition maintains the integrity of the microvascular barrier during leukocyte extravasation. Inhibition of neutrophil-derived microparticle formation resulted in dramatically increased vascular leakage. These findings suggest that deposition of microparticles during neutrophil extravasation is essential for maintaining endothelial barrier function and may result in temporal difference between neutrophil extravasation and an increase in vascular leakage.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7703-7705
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• 2014

Cancer-testis (CT) antigens are a group of tumor-associated antigens with restricted expression in normal tissues except for testis and expression in a wide variety of tumor tissues. This pattern of expression makes them suitable targets for immunotherapy as well as potential biomarkers for early detection of cancer. However, some genes attributed to this family are now known to be expressed in other normal tissues which put their potential applications in immunotherapy and cancer detection under question. Here we analyzed expression of two previously known CT antigens, RHOXF2 and PIWIL2, in AML patients versus normal donors and found no significant difference in the expression of these genes between the two groups. As these two genes showed expression in normal leukocytes, their expression pattern seems to be wider than to be attributed to the CT gene family. Future research should focus on the expression profiles of so called CT antigens to find those with more testis specific expression.

• 한국수산과학회지
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• 제45권6호
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• pp.675-678
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• 2012

The study examined the effect of levamisole on the natural cytotoxic cell (NCC) activity of Nile tilapia (Oreochromis niloticus) head kidney (HK) leukocytes. In vitro, HK leukocytes were incubated with $10^{-4}$ to $10^4$ ng of levamisole/ml for 5, 20 or 40 h and then their NCC activity against target cells was assayed. The NCC activity appeared to increase after a 20 h of incubation. In vivo, tilapia were fed commercial diet containing 0, 50, 100 or 300 mg of levamisole/kg for 12 consecutive days. Then, the fish were fed a commercial normal diet and samples harvested weekly 0 to 8 weeks after levamisole administration. The NCC activity was augmented uniformly at all times examined until the end of the experiment. In conclusion, levamisole effectively enhanced and maintained tilapia NCC activity.