Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Leukocyte subpopulations of peripheral blood in Korean indigenous cattle vaccinated with attenuated live Akabane virus vaccine

아카바네 생 바이러스 백신 접종에 의한 한우 백혈구 아군의 변화

  • 박영남 (강원도가축위생시험소) ;
  • 손승미 (강원도가축위생시험소) ;
  • 신명균 (강원도가축위생시험소) ;
  • 권명상 (강원대학교 수의학부대학)
  • Published : 2006.09.01
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Abstract

To identify immune response of leukocytes in peripheral blood of cattle vaccinated with an attenuated live Akabane virus vaccine, leukocytes were reacted with monoclonal antibodies which are specific to bovine lymphocyte surface antigens and assayed by the flow cytometry. Serum neutralizing (SN) test was used to measure antibody titers after vaccination, SN antibody was appeared to 7 days post-vaccination (PV) and 2-8 antibody titers were observed in 14 days PV. Proportion of $CD8^-$ MHC $class II^+$ expressing cells were rapidly increased at 3 days PV. $CD8^+$ MHC $class II^-$ cells were increased at 7 days PV. $CD4^+CD8^-,\;WC^+CD4^-,\;CD4^+CD8^+,\;WC1^-CD4^+, \;WC1^-CD8^+$, and $CD4^-CD8^+$ cells were highly increased at 3, 3, 7, 7, 14, 14 days PV, respectively.

Keywords

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