Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Levamisole on Enhancing Natural Cytotoxic Cell Activity in Nile Tilapia Oreochromis niloticus

레바미졸이 틸라피아(Oreochromis niloticus)의 자연살해세포 활성에 미치는 영향

  • Park, Kwan-Ha (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Yoon, Jong-Man (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sanghoon (Department of Aquatic Life Medicine, Kunsan National University)
  • 박관하 (군산대학교 수산생명의학과) ;
  • 윤종만 (군산대학교 수산생명의학과) ;
  • 최상훈 (군산대학교 수산생명의학과)
  • Received : 2012.09.13
  • Accepted : 2012.12.20
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The study examined the effect of levamisole on the natural cytotoxic cell (NCC) activity of Nile tilapia (Oreochromis niloticus) head kidney (HK) leukocytes. In vitro, HK leukocytes were incubated with $10^{-4}$ to $10^4$ ng of levamisole/ml for 5, 20 or 40 h and then their NCC activity against target cells was assayed. The NCC activity appeared to increase after a 20 h of incubation. In vivo, tilapia were fed commercial diet containing 0, 50, 100 or 300 mg of levamisole/kg for 12 consecutive days. Then, the fish were fed a commercial normal diet and samples harvested weekly 0 to 8 weeks after levamisole administration. The NCC activity was augmented uniformly at all times examined until the end of the experiment. In conclusion, levamisole effectively enhanced and maintained tilapia NCC activity.

Keywords

References

  1. Anderson DP. 1992. Immunostimulants, adjuvants, and vaccine carriers in f ish: applications to aquaculture. Ann Rev Fish Dis 2, 281-307. https://doi.org/10.1016/0959-8030(92)90067-8
  2. Baba T, Watase Y and Yoshinaga Y. 1993. Activation of mononuclear phagocyte function by levamisole immersion in carp. Nippon Suisan Gakkaishi 59, 301-307. https://doi.org/10.2331/suisan.59.301
  3. Cabaj W, Stankiewicz M, Jomas WE and Moore LG. 1995. Levamisole and its influence on the immune response of lambs. Vet Res Commun 19, 17-26. https://doi.org/10.1007/BF01839248
  4. Cheng YC and Po HL. 2011. Leukoencephalopathy after levamisole for the treatment of verrucae. Acta Neurol Taiwan 4, 262-266.
  5. Cuesta A, Esteban MA and Meseguer J. 1999. Natural cytotoxic activity of giltheas seabream (Sparus aurata L) leucocytes. Assessment by f low cytometry and microscopy. Vet Immunol Immunopathol 71, 161-171. https://doi.org/10.1016/S0165-2427(99)00063-X
  6. Kajita Y, Sakai M, Atsuta S and Kobayashi M. 1990. The immunomodulatory effects of levamisole on rainbow trout, Oncorhynchus mikiss . Fish Pathol 25, 93-98. https://doi.org/10.3147/jsfp.25.93
  7. Mulero V, Esteban MA and Meseguer J. 1998b. In vitro levamisole fails to increase seabream (Sparus aurata L) phagocyte functions. Fish Shellf ish Immunol 8, 315-318. https://doi.org/10.1006/fsim.1998.0141
  8. Mulero V, Esteban MA, Munoz J and Meseguer J. 1998a. Dietary intake of levamisole enhances the immune response and disease resistance of the marine teleost gilthead seabream (Sparus aurata L) Fish Shellfish Immunol 8, 49-62. https://doi.org/10.1006/fsim.1997.0119
  9. Olivier G, Evelyn TPT and Lallier R. 1985. Immunity to Aeromonas salmonicida in coho salmon (Oncorhynchus kisuthch ) induced by modif ied Freund's complete adjuvant: its non-specific nature and the probable role of macrophages in the phenomenon. Dev Comp Immunol 9, 419-432. https://doi.org/10.1016/0145-305X(85)90005-9
  10. Renoux G. 1980. The general immunopharmacology of levamisole. Drugs 19, 89-99.
  11. Sakai M. 1999. Current research status of f ish immunostimulants. Aquaculture 172, 63-92. https://doi.org/10.1016/S0044-8486(98)00436-0
  12. Siwicki AK. 1987. Immunomodulating activity of levamisole in carp spawners, Cyprinus carpio . J Fish Biol 31, 245-246. https://doi.org/10.1111/j.1095-8649.1987.tb05325.x
  13. Siwicki AK. 1989. Immunomodulating inf luence of levamisole on nonspecific immunity in carp (Cyprinus carpio ). Dev Comp Immunol 13, 87-91. https://doi.org/10.1016/0145-305X(89)90021-9
  14. Siwicki AK, Anderson DP and Dixon OW. 1990. In vitro immunostimulation on rainbow trout (Oncorhynchus mikiss ) spleen cells with levamisole. Dev Comp Immunol 14, 231-237. https://doi.org/10.1016/0145-305X(90)90094-U
  15. Siwicki AK and Cossarini-Dunier M. 1990. Effect of levamisole on the lymphocyte and macrophage activity in carp (Cyprinus carpio ). Ann Rech Vet 21, 95-100.