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Genetic Diversity of Endangered Fish Hemibarbus mylodon (Cyprinidae) Assessed by AFLP (AFLP 분석에 의한 어름치 Hemibarbus mylodon의 유전 다양성)

  • Lee, Yoon-A;Yun, Young-Eun;Nam, Yoon-Kwon;Bang, In-Chul
    • Journal of Aquaculture
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    • v.21 no.3
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    • pp.196-200
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    • 2008
  • Korean spotted barbel Hemibarbus mylodon, is an endangered and endemic freshwater species in the Korean peninsula. Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity and population genetic structure of three populations (Bukhan, Namhan and Imjin-river). Fifteen AFLP primer pairs produced 795 products of which 135 were polymorphic(17%). Percentages of polymorphic bands were similar among the three populations, with accounting 11.9%, 11.1%, and 13.4% for Bukhan, Namhan and Imjin-river populations, respectively. An average genetic similarity among the three populations was 0.969. The average heterozygosity (0.033-0.040) and genetic diversity (0.036-0.043) were significantly low. Pairwise distance and fixation index analyses of three populations also suggested quite a low genetic differentiation one another. These results would provide a fundamental baseline data to develop the effective strategy for the management and restoration of this endangered fish species.

Habitat Environment, Age and Feeding Ecology of the Endangered Species, Gobiobotia macrocephala (Pisces: Cyprinidae) in the Seom River, Korea (멸종위기 어류 꾸구리 Gobiobotia macrocephala (Pisces: Cyprinidae)의 서식환경과 연령, 섭식생태)

  • Ko, Myeong-Hun;Song, Ha-Yoon;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.23 no.4
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    • pp.278-287
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    • 2011
  • Habitat environment, age and feeding ecology of Gobiobotia macrocephala were investigated to provide baseline data for ecological characteristics and recovery in the Seom River at Heungho- ri, Buron-myeon, Wonju-si, Gangwon-do, Korea from 2010 to 2011. G. macrocephala inhabited 10~40 cm in water depth, 8~20 cm bottom size and 40~120 cm/sec in current velocity, and pebble floor were factors to be important for inhabit density. Age group of G. macrocephala in May estimated from the standard length indicated that the 28~42 mm group is 1 year old, the 43~58 mm group is 2 years old, the 59~69 mm group is 3 years old and the 69~85 mm group is more than 4 years old, and the female was 4~10 mm larger than the male. G. macrocephala did mainly feeding with nocturnal fishes between 00 h to 06 h, and they fed mainly Trichoptera (57.7%), Diptera (26.0%) and Ephemeroptera (16.3%). And their small juvenile fed mainly Ephemeroptera and Diptera, however, they ate mainly Trichoptera while growing to adult fish.

The Effect of a Smoke-free Campus Policy on Receptivity of Campus and Government Smoke-free Policy among College Students (캠퍼스 금연정책이 대학생들의 캠퍼스 내·외 금연정책 수용성에 미치는 영향)

  • Shin, Sung-Rae;Kim, Yun-Hee
    • The Journal of the Korea Contents Association
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    • v.17 no.9
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    • pp.363-374
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    • 2017
  • This study was performed to evaluate the effect of a smoke-free campus policy on college students' receptivity of campus and governmental smoke free policy. The current study utilized pre and post cross-sectional survey in length of nine months. Data were collected from similar major freshmen at two pre matched universities, one representing smoke-free campus in Seoul city and one representing non smoke-free campus in Busan city, Korea. Baseline data were collected in March 2016 and follow-up data were collected in December 2016. No differences were found between initial and follow-up data on receptivity of smoke-free campus policy in both university students revealing that the smoke-free campus policy does not influence in changing student's attitude towards unfavorable direction. Receptivity on governmental smoke free policy demonstrated significant changes among participants in non smoke-free campus. Their receptivity level changed significantly toward unfavorable direction. The implementation of a smoke-free campus policy can give positive impact on students' receptivity of campus as well as governmental smoke free policy.

Influencing factors for abrasive flow rate and abrasive flow quality of abrasive injection waterjet systems for tunnel excavation (터널굴착용 투입형 연마재 워터젯 시스템의 연마재 투입량과 유동성에 미치는 영향 인자)

  • Joo, Gun-Wook;Oh, Tae-Min;Cho, Gye-Chun
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.16 no.4
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    • pp.417-430
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    • 2014
  • A new rock excavation method using an abrasive waterjet system is under development for efficiently creating tunnels and underground spaces in urban areas. In addition, an appropriate abrasive flow rate and abrasive flow quality are important for the new rock excavation (cutting) method using an abrasive waterjet system. This study evaluated the factors influencing the abrasive flow rate and abrasive flow quality, specifically the abrasive pipe height, length, tortuosity and inner diameter, through experimental tests. Based on the experimental test results, this study suggested optimal conditions for the abrasive flow rate and abrasive flow quality. The experimental results can be effectively utilized as baseline data for rock excavation methods using an abrasive waterjet system in various construction locations such as tunnels near urban surroundings, utility tunnels, and shafts.

Secondary Metabolites and Morphological Diversity in the Leaves of Perilla Landrace from Korea

  • Assefa, Awraris Derbie;Sung, Jung-Sook;Jeong, Yi-Jin;Lee, Ho-Sun;Rhee, Ju-Hee;Hur, On-Sook;Noh, Jae-Jong;Ro, Na-Young;Hwang, Ae-Jin;Lee, Jae-Eun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.64-64
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    • 2019
  • Screening and identification of genetic resources based on their phytoconstituents and morphological characters potentially provide baseline data for researchers, breeders, and nutraceutical companies who wish to formulate a nutrient-dense diet and health beneficial supplement. Thus, we evaluated the amount of total phenolic content and major phenolic compounds; examined if phenolic compounds could be used as distinguishing factors for perilla genetic resources; and investigated the relation between some quantitative and qualitative morphological characters with the contents of phenolic compounds in 360 accessions obtained from National Agrobiodiversity Center gene bank, Jeonju, Korea. Total phenolic content (TPC) was estimated using Folin-Ciocalteu colorimetric assay. Individual phenolic compounds were determined using an Ultra-High Performance Liquid Chromatography system equipped with Photodiode Array detector. Considerable variations were observed in TPC (7.99 to 117.47 mg GAE/g DE), rosmarinic acid (RA) (ND to 19.19 mg/g DE), caffeic acid (CA) (ND to 0.72 mg/g DE), apigenin-7-O-diglucuronide (ADG) (ND to 1.24 mg luteolin equivalent (LUE)/g DE), scutellarein-7-O-glucuronide (SG) (ND to 4.32 mg LUE/g DE), and apigenin-7-O-glucuronide (AG) (ND to 1.60 mg LUE/g DE). RA was the most dominant phenolic compound in most accessions (95.3%) followed by SG. The adaxial leaf color was light green, green and dark green in 13.8%, 65.0%, and 21.1 % of the accessions, respectively. 78.8% of the accessions had light green color at the abaxial side with the remaining being described as green. Most of the accessions (96.9%) were cordate shape, the remaining being eclipse. Intensities of green pigment at abaxial and adaxial leaf surfaces were correlated with contents of individual phenolic compounds and TPC whereas leaf length and width had no correlation with TPC, CA and RA, and negatively correlated with ADG, AG, and SG. Leaf shape was not related with content of phenolic compounds, color of leaves, or the length or width of leaves. Accessions IT57426, IT157434, IT267710, and IT267712 which contained relatively high contents of TPC and major phenolic compounds (RA and SG) could be used for further research in breeding and bioassay test. Our study result showed the contents of total phenolics and individual phenolic compounds along with the morphological characters could be useful distinguishing factors for perilla genetic resources.

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Occurrence of Regalecus russellii off the Coast of Gangwon-do, Korea and Coastal Environment (강원도 속초 연안에서 산갈치(Regalecus russellii) 출현과 연안환경)

  • Jong-Won Park;Soon-Man Kwon;Pyo-Il Han;Chung Il Lee
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.29 no.5
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    • pp.520-524
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    • 2023
  • Regalecus russellii, which spends most of its life in the deep sea, occasionally appears in coastal waters. However, the reasons for its appearance remain unclear. In Korea, R. russellii mainly appears along the eastern coastal waters, and most of them are caught in fishing gear, such as gill nets, or are stranded on the shore; nevertheless, the frequency of appearance is extremely low. Even if found, this species is often identified to be morphologically similar to Trachipterus ishikawae, and comprehensive analysis to identify the species through sample collection is limited. Consequently, information on the biological characteristics of R. russellii appearing in the coastal waters of Korea is scarce. Herein, the anatomical characteristics of R. russellii caught in a gill net off the Gangwon-do coast on March 14, 2023, were analyzed, and coastal water temperature was measured using an ocean buoy. Our results showed that the individual was male, its total length was 320 cm, body weight was 27.52 kg, body length was 26.62 cm, gonad weight was 619.45 g, and liver weight was 218.71 g. The stomach was full of euphausiids. The water temperature changed drastically at 15-30 m roughly a week before the R. russellii individual was caught, and the subsurface water temperature was lower than 10 ℃. Our findings provide baseline data to understand the ecological characteristics of R. russellii appearing along the eastern coast of the Korea.

Comparisons of Clinical Characteristics and Outcomes in COPD Patients Hospitalized with Community-acquired Pneumonia and Acute Exacerbation (만성폐쇄성폐질환에 폐렴과 급성악화로 입원한 환자의 임상적 특성 및 예후 비교)

  • Jeong, Seung-Wook;Lee, Jae-Hee;Choi, Keum-Ju;HwangBo, Yup;Kim, Yi-Young;Lee, Yun-Ji;Yoon, Won-Kyung;Kim, Min;Cha, Sung-Ick;Park, Jae-Yong;Jung, Tae-Hoon;Kim, Chang-Ho
    • Tuberculosis and Respiratory Diseases
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    • v.69 no.1
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    • pp.31-38
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    • 2010
  • Background: Data comparing the clinical characteristics and outcomes in chronic obstructive pulmonary disease (COPD) patients hospitalized with community-acquired pneumonia (CAP-COPD) and acute exacerbation (AECOPD) are very limited. Methods: Eighty episodes of hospitalization in 65 CAP-COPD patients, and 111 episodes of hospitalization in 82 AE-COPD patients were included in this study. The baseline characteristics, clinical presentations, potential bacterial pathogens and clinical outcomes in these patients were retrospectively reviewed and compared. Results: No significant differences were found between the two groups in parameters related to COPD and co-morbidities, except a higher rate of male among CAP-COPD patients. Clinical presentations by symptoms and laboratory findings on admission were significantly more severe in CAP-COPD patients, who showed higher rates of fever and crepitation, but less wheezing than AE-COPD patients. S. pneumoniae and P. aeruginosae were the most common bacterial pathogens in both groups. With no difference in the overall hospital mortality between both groups, the mean length of hospital stay was significantly longer in the CAP-COPD patients than in AE-COPD patients (15.3 vs. 9.8 days, respectively, p<0.01). Additional analysis on CAP-COPD patients showed that systemic steroid use did not influence the length of hospital stay. Conclusion: Although there was no significant difference in bacterial pathogens and overall hospital mortality between the two groups, CAP-COPD patients had more severe clinical symptoms and laboratory findings at presentation, and longer hospital stay than AE-COPD patients.

Phenotypic Characterization of Aseel Chicken of Bangladesh

  • Sarker, Md. Jonaed Alam;Bhuiyan, Mohammad Shamsul Alam;Faruque, Md. Omar;Ali, Md. Ashraf;Lee, Jun-Heon
    • Korean Journal of Poultry Science
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    • v.39 no.1
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    • pp.9-15
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    • 2012
  • The aim of this study was to investigate phenotypic characteristics, morphometric measurements, reproduction and production performances of Aseel chicken of Bangladesh. The dominant feather color of neck/hackles was red in both males (56.14%) and females (54.16%) while the sickle feather color was mostly black in both chickens (71.93% vs. 54.17%). The predominant saddle and breast feather colors were red (40.35%) and black (64.91%), respectively, in male whereas most frequent observed color was pale brown in female (58.33 and 50.0%, respectively). The predominant feather color of wing bow and wing bay was found black (68.42 and 80.70%, respectively) in male but only pale brown color was observed in females (62.5 and 54.17%, respectively) for these two characters. Different phenotypic measurements such as the average shank length and circumference were $12.79{\pm}0.13$ and $7.8{\pm}0.08$ cm, respectively, in male and $10.21{\pm}0.25$ and $5.81{\pm}0.21$ cm, respectively, in female. Keel length was $14.39{\pm}0.19$ cm in male and $10.79{\pm}0.23$ cm in female. The average adult live weight in male was measured $3749.12{\pm}83.44$ g while in female it was $2062.50{\pm}105.26$ g. The age of 1st lay was found to be 28.86 weeks. Total number of eggs laid per year ranged between 24~48, number of clutch/hen/year varied from 2 to 4 and number of eggs/clutch/hen was found to be 10~12. The average live weight of Aseel chicken at 1, 2, 3, 4, 6, 8, 10, 12, 16 and 17 weeks of age were recorded as $31.14{\pm}0.55$, $48.63{\pm}3.99$, $116.57{\pm}5.72$, $138.40{\pm}5.91$, $212.88{\pm}4.82$, $361.00{\pm}9.72$, $577.50{\pm}42.86$, $743.75{\pm}24.65$, $1086.00{\pm}26.02$, $1402.00{\pm}24.54$ and $1432.00{\pm}27.00$ g respectively. Finally, this phenotypic characterization as well as productive and reproductive performances of Aseel chicken will give the baseline information to researcher for further study and for planning any on-ward conservation and implement strategy.

Life History of the Endangered Endemic Species, Rhynchocypris semotilus (Pisces: Cyprinidae) in the Songhyeoncheon (Stream), Korea (송현천에 서식하는 멸종위기어류 버들가지 Rhynchocypris semotilus (Pisces: Cyprinidae)의 생활사)

  • Myeong-Hun Ko;Mee-Sook Han ;Hyung Soo Seo
    • Korean Journal of Ichthyology
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    • v.35 no.3
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    • pp.160-170
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    • 2023
  • Life history of the endangered endemic species, Rhynchocypris semotilus, was investigated to obtain baseline data for its ecological characteristics in Songhyeoncheon, Hyeonnae-myeon, Goseong-gun, Gangwon-do, Korea from April to October, 2022. A total of 10 families and 18 species of fishes were collected from 4 survey stations, and R. semotilus was a subdominant species in the survey period (seven times). The main habitat of R. semotilus was the middle-upper stream of rock and boulder bottoms with aquatic plants and slow rapids and pools. The age groups for R. semotilus estimated by the frequency distribution of total length in spawning season (May) indicated the 4~12 mm is 0-year old, 32~49 mm is 1-year old, the 50~65 mm group is 2-years old, the 66~91 mm is 3-years old, and the 92~116 mm is more than 4-years old, and showed rapid growth from May to September. Total length range of female and male were the relatively similar, and sex ratio (♂/♀) was 0.78. The spawning period is from May to July (water temperature is 17 to 21℃), and gonad was developed more than three years old (more than TL 66 mm). In May, the gonadosomatic index (GSI) was 10.6±2.99% for females and 8.2±2.34% for males, fecundity was 6,830±1,943, and mature eggs size was 1.34±0.16 mm. Finally, life history characteristics of R. semotilus was discussed between the genus Rhynchocypris and Phoxinus species.

Unplanned Readmission to Intensive Care Unit during the same Hospitalization at a Teaching Hospital (계획에 없던 중환자실 재입실 실태 및 원인)

  • Song, Dong-Hyun;Lee, Sun-Gyo;Kim, Chui-Gyu;Choi, Dong-Ju;Lee, Sang-Il;Park, Su-Kil
    • Quality Improvement in Health Care
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    • v.10 no.1
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    • pp.28-41
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    • 2003
  • Background : Because unplanned readmissions to intensive care unit(ICU)might be related with undesirable patient outcomes, we investigated the pattern of and reason for unplanned ICU readmission to provide baseline data for reducing unplanned returns to ICU. Methods : The subjects included all patients who readmitted to ICU during the same hospitalization at a tertiary referral hospital between January 1st and June 30th 2002. Quality improvement(QI) nurse collected the data through medical records and a medical director reviewed the data collected. Results : 1) The average unplanned ICU readmission rate was 5.6%(gastroenterology 14.6%, pediatrics 12.7%, pulmonology 11.9%, neurosurgery 6.3%, general surgery 5.3%, chest surgery 3.9%, and cardiology 3.3%). 2) Among the unplanned readmissions, more than 50% of cases were from patients older than 60 years, and the main categories of diagnose at hospital admission were neurologic disease(29.9%) and cardiovascular disease(27.6%). 3) Of unplanned ICU readmissions, 41.8% had recurrence of the initial problems, 44.8% had occurrence of new problems. And 9.7% required post-operative care after unplanned operations. 4) The most common cause responsible for unplanned ICU readmission were respiratory problem(38.3%) and cardiovascular problem(14.3%). 5) About 40% of unplanned ICU readmission occurred within 3 days after ICU discharge. 6) Average length of stay of the readmitted patients to ICUs were much longer than that of non-readmitted patients. 7) Hospital mortality rate was much higher for unplanned ICU readmitted patients(23.6%) than for non-readmitted patients(1.5%) (P<0.001). Conclusions : This study showed that the unplanned ICU readmitted patients had poor outcomes(high morality and increased length of stay). In addition study results suggest that more attention should be paid to patients in ICU with poor respiratory function or elderly patients, and careful clinical decisions are required at discharged from ICU to general ward.

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