Kim, Su-Jin;Lee, Jae-Sung;Lee, Won-Woo;Kim, Yu-Kyeong;Jang, Sung-June;Son, Kyu-Ri;Kim, Hyo-Cheol;Chung, Jin-Wook;Lee, Dong-Soo
Nuclear Medicine and Molecular Imaging
/
v.41
no.4
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pp.317-325
/
2007
Purpose: Biological parameters can be quantified using dynamic PET data with compartment modeling and Nonlinear Least Square (NLS) estimation. However, the generation of parametric images using the NLS is not appropriate because of the initial value problem and excessive computation time. In irreversible model, Patlak graphical analysis (PGA) has been commonly used as an alternative to the NLS method. In PGA, however, the start time ($t^*$, time where linear phase starts) has to be determined. In this study, we suggest a new Multiple Linear Analysis for irreversible radiotracer (MLAIR) to estimate fluoride bone influx rate (Ki). Methods: $[^{18}F]Fluoride$ dynamic PET scans was acquired for 60 min in three normal mini-pigs. The plasma input curve was derived using blood sampling from the femoral artery. Tissue time-activity curves were measured by drawing region of interests (ROls) on the femur head, vertebra, and muscle. Parametric images of Ki were generated using MLAIR and PGA methods. Result: In ROI analysis, estimated Ki values using MLAIR and PGA method was slightly higher than those of NLS, but the results of MLAIR and PGA were equivalent. Patlak slopes (Ki) were changed with different $t^*$ in low uptake region. Compared with PGA, the quality of parametric image was considerably improved using new method. Conclusion: The results showed that the MLAIR was efficient and robust method for the generation of Ki parametric image from $[^{18}F]Fluoride$ PET. It will be also a good alternative to PGA for the radiotracers with irreversible three compartment model.
Q estimates are made by direct measurements of energy loss per cycle from primary P and S waves, as a function of frequency. Assuming that intrinsic Q is frequency independent and scattering Q is frequency dependent over the frequencies of interest, the relative contributions of each, to a total observed Q, may be estimated. Test examples are produced by computing viscoelastic synthetic seismograms using a pseudo spectral solution with inclusion of relaxation mechanisms (for intrinsic Q) and a fractal distribution of scatterers (for scattering Q). The composite theory implies that when the total Q for S-waves is smaller than that for P-waves (the usual situation), intrinsic Q is dominating; when it is larger, scattering Q is dominating. In the inverse problem, performed by a global least squares search, intrinsic $Q_p$ and $Q_s$ estimates are reliable and unique when their absolute values are sufficiently low that their effects are measurable in the data. Large $Q_p$ and $Q_s$ have no measurable effect and hence are not resolvable. Standard deviation of velocity $({\sigma})$ and scatterer size (A) are less unique as they exhibit a tradeoff as predicted by Blair's equation. For the P-waves, intrinsic and scattering contributions are of approximately the same importance, for S-waves, the intrinsic contributions dominate.
Numerous methods have been applied to assess the antibacterial effectiveness of hand hygiene products. However, the different results obtained through various evaluation methods have complicated our understanding of the real efficacy of the products. Few studies have compared test methods for assessing the efficacy of hand hygiene products. In particular, reports on ex vivo pig skin testing are limited. This study aimed to compare and characterize the methodologies applied for evaluating hand hygiene products, involving in vitro, ex vivo, and in vivo approaches, applicable to both leave-on sanitizers and wash-off products. Our further aim was to enhance the reliability of ex vivo test protocols by identifying influential factors. We performed an in vitro method (EN1276) and an in vivo test (EN1499 and ASTM2755) with at least 20 participants, against Serratia marcescens or Escherichia coli and Staphylococcus aureus. For the ex vivo experiment, we used pig skin squares prepared in the same way as those used in the in vivo test method and determined the optimal treated sample volumes for sanitizers and the amount of water required to wash off the product. The hand sanitizers showed at least a 5-log reduction in bacterial load in the in vitro test, while they showed little antibacterial activity in the in vivo and ex vivo tests, particularly those with a low alcohol content. For the hand wash products, the in vitro test was limited because of bubble formation or the high viscosity of the products and it showed low antibacterial activity of less than a 1-log reduction against E. coli. In contrast, significantly higher log reductions were observed in ex vivo and in vivo tests, consistently demonstrating these results across the two methods. Our findings revealed that the ex vivo and in vivo tests reflect the two different antibacterial mechanisms of leave-on and wash-off products. Our proposed optimized ex vivo test was more rapid and more precise than the in vitro test to evaluate antibacterial results.
This study was conducted to determine the differences in the growth and milk production performance of Holstein Crossbreds (Korean Native Cattle(♀)${\times}$Holstein(♂)) and Korean Native Cattle produced at the Livestock Experiment Station of the Office of Rural Developement from 1973 to 1978. The number of heifers and cows used in this experiment were 15 head of Korean Native Cattle and 11 head pf Holstein Crossbreds Cattle. Body weight and body measurements were taken at birth, 6, 12, 24 and 36 months of age, however, body measurements were not taken at birth. Milk production was checked from the 11 th day to 180th day after calving. The data was analyzed using the least square procedure in order to estimate the effect of the mating group, year of birth, calving season and parity. The results obtained from this study were as follows: 1. The body weights of the Holstein Crossbreds were heavier than the body weights of purebred Korean Native Cattle. The body weight of the Holstein Crossbreds averaged 28.09kg, 146.64kg, 254.48kg, 392.04kg and 454.46kg at birth, 6, 12, 24 and 36 months of age, respectively. However, the body weights of purebreds Korean Native Cattle averaged 22.45kg, 132.82kg, 220.68kg, 363.54kg and 365.54kg at the same ages. 2. The year of birth affected on body weight at each point during the growing stage, except birth, heifers born in the spring and autumn were heavier than the others, but calving season did not affect on body weight during the growing stage except at birth and 6 months. 3. Parity showed significant differences on body weight in the growing stage. Calves from the 5th parity had a tendency to be heavier than the other calves. 4. The year of birth, calving season and parity at calving had no affect on the change of body measurements, but the wither height, hip height, chest depth, chest girth and hip width were significantly greater in the Holstein Crossbreds at 24 months of age. 5. Mating groups had a significant affect on milk production during the growing stage. Year of birth and calving season did not affect milk production, but parity was significant from 11 days after calving. 6. The least-squares means used to determine the daily milk production were 3.60 and 8.26kg/day for Korean Native Cattle and the Holstein Crossbreds, respectively.
Kim, Hyun-Chul;Noh, Jae-Koo;Lee, Jeong-Ho;Kim, Jong-Hyun;Park, Choul-Ji;Kang, Jung-Ha;Kim, Kyung-Kil;Lee, Jung-Gyu;Myeong, Jeong-In
Journal of Aquaculture
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v.21
no.4
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pp.317-324
/
2008
This study estimated the genetic parameters and breeding values for the growth-related traits of the 1st generation produced in 2005. The heritability of body weight, total length, body height, body shape and condition factor of 180 days old olive flounders Paralichthys olivaceus, the 1st generation of selection, was estimated as 0.564, 0.590, 0.588, 0.306 and 0.332, respectively. And reproductivity of genetic evaluation for crossing superior flounders and inferior ones was tested using the subsequent generation produced in 2006 based on the breeding values of 1st generation. In the least-squares means of body weight and total length for each group of crossing, the values of crossing group between superior flounders ($S{\times}S$) showed $145.6{\pm}1.8\;g$ and $22.4{\pm}0.1\;cm$, respectively. The values of crossing group between superior and inferior flounders ($S{\times}I$) showed $133.2{\pm}2.5\;g$ and $22.1{\pm}0.1\;cm$, respectively. The values of crossing group between inferior flounders ($I{\times}I$) showed $114.0{\pm}2.1\;g$ and $21.08{\pm}0.12\;cm$, respectively. In the results, flounders are determined as appropriate selective breeding fish with the high heritability of flounders in early ages at 180 days old, and the reproductivity of genetic evaluation was also high.
Color is one of the quality determining factors for pepper powder. To measure the color of pepper powder, several methods including high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and ASTA-20 have been used. Among the methods, the ASTA-20 method is most widely used for color measurement of a large number of samples because of its simplicity and accuracy. However it requires time consuming preprocessing steps and generates chemical waste containing acetone. As an alternative, we developed a fast and simple method based on a visible/near infrared (Vis/NIR) hyperspectral method to measure the color of pepper powder. To evaluate correlation between the ASTA-20 and the visible/near infrared (Vis/NIR) hyperspectral methods, we first measured the color of a total of 488 pepper powder samples using the two methods. Then, a partial least squares (PLS) model was postulated using the color values of randomly selected 3 66 samples to predict ASTA values of unknown samples. When the ASTA values predicted by the PLS model were compared with those of the ASTA-20 method for 122 samples not used for model development, there was very high correlation between two methods ($R^2=0.88$) demonstrating reliability of Vis/NIR hyperspectral method. We believe that this simple and fast method is suitable for highthroughput screening of a large number of samples because this method does not require preprocessing steps required for the ASTA-20 method, and takes less than 30 min to measure the color of pepper powder.
The epidemic of disorders associated with synthetic stimulants, such as methamphetamine (MA) and amphetamine (AP), is a health, social, legal, and financial problem. Owing to the high potential of their abuse and addiction, reliable analytical methods are required to detect and identify MA, AP, and their metabolites in biological samples. Thus, a dilute-and-shoot liquid chromatography-tandem mass spectrophotometry (LC-MS/MS) was developed for simultaneous determination of MA, 4-hydroxymethamphetamine (4HMA), AP, and 4-hydroxyamphetamine (4HA) in urine. Urine sample ($100{\mu}L$) was mixed with $50{\mu}L$ of mobile phase consisting of 0.4 % formic acid and methanol and $50{\mu}L$ of working internal-standard solution. Aliquots of $8{\mu}L$ diluted urine was injected into the LC-MS/MS system. For all analytes, chromatographic separation was performed using a C18 reversed-phase column with gradient elution and a total run time of 5 min. The identification and quantification were performed by multiple reaction monitoring (MRM). Linear least-squares regression was conducted to generate a calibration curve, with $1/x^2$ as the weighting factor. The linear ranges were 2.0-200, 1.0-800, and 10-2500 ng/mL for 4HA and 4HMA, AP, and MA, respectively. The inter- and intraday precisions were within 6.6 %, whereas the inter- and intraday accuracies ranged from -14.9 to 11.3 %. The low limits of quantification were 2.0 ng/mL (4HA and 4HMA), 1.0 ng/mL (AP), and 10 ng/mL (MA). The proposed method exhibited satisfactory selectivity, dilution integrity, matrix effect, and stability, which are required for validation. Moreover, the purification efficiency of high-speed centrifugation was clearly higher than 6-15 % for QC samples (n=5), which was higher than that of the membrane-filtration method. The applicability of the proposed method was tested by forensic analysis of urine samples from drug abusers.
The standardization of marketing has been one of the most focused research topics in international marketing. The term "global marketing" was often used to mean an internationally standardized marketing strategy based on similarities between foreign markets. Marketing standardization was discussed only within the context of traditional physical marketplaces. Since then, the digital "marketspace" of the Internet had emerged in the 90's, and it became one of the most important drivers of the globalization process opening new opportunities for the standardization of global marketing activities. On the other hand, the opinion that a greater adoption of the Internet by customers may lead to a higher degree of customization and differentiation of products rather than standardization is also quite popular. Considering this disagreement, it is notable that comprehensive studies which focus upon the marketing standardization especially in the context of global e-commerce are missing to a high degree. On this background, the two basic research questions being addressed in this study are: (1) To what extent do companies standardize their marketing in international e-commerce? (2) Is there an impact of marketing standardization on the performance (or success) of these companies? Following research hypotheses were generated based upon literature review: H 1: Internationally engaged e-commerce firms show a growing readiness for marketing standardization. H 2: Marketing standardization exerts positive effects on the success of companies in international e-commerce. H 3: In international e-commerce, marketing mix standardization exerts a stronger positive effect on the economic as well as the non-economic success of companies than marketing process standardization. H 4: The higher the non-economic success in international e-commerce firms, the higher the economic success. The data for this research were obtained from a questionnaire survey conducted from February to April 2005. The international e-commerce companies of various industries in Germany and all subsidiaries or headquarters of foreign e-commerce companies based in Germany were included in the survey. 118 out of 801 companies responded to the questionnaire. For structural equation modelling (SEM), the Partial-Least. Squares (PLS) approach in the version PLS-Graph 3.0 was applied (Chin 1998a; 2001). All of four research hypotheses were supported by result of data analysis. The results show that companies engaged in international e-commerce standardize in particular brand name, web page design, product positioning, and the product program to a high degree. The companies intend to intensify their efforts for marketing mix standardization in the future. In addition they want to standardize their marketing processes also to a higher degree, especially within the range of information systems, corporate language and online marketing control procedures. In this study, marketing standardization exerts a positive overall impact on company performance in international e-commerce. Standardization of marketing mix exerts a stronger positive impact on the non-economic success than standardization of marketing processes, which in turn contributes slightly stronger to the economic success. Furthermore, our findings give clear support to the assumption that the non-economic success is highly relevant to the economic success of the firm in international e-commerce. The empirical findings indicate that marketing standardization is relevant to the companies' success in international e-commerce. But marketing mix and marketing process standardization contribute to the firms' economic and non-economic success in different ways. The findings indicate that companies do standardize numerous elements of their marketing mix on the Internet. This practice is in part contrary to the popular concept of a "differentiated standardization" which argues that some elements of the marketing mix should be adapted locally and others should be standardized internationally. Furthermore, the findings suggest that the overall standardization of marketing -rather than the standardization of one particular marketing mix element - is what brings about a positive overall impact on success.
The combination of early heading time, maturing time and short grain-filling period is very important to develop early varieties in winter barley. The 4 parental half diallel crosses (parents, $F_1$s, $F_2$s) were cultivated at the field. The heading date was from April 3 to 26, maturing date from May 15 to 27 and grain-filling period from 31 days to 42 days, showing that the varietal differences about the 3 traits were remarkable. According to half diallel cross analyses, Dongbori 1 for heading time (late heading) was dominant, but Oweolbori (early heading) was recessive, showing partial dominance with high additive component of genetic variance. Dongbori 1 for maturing time was dominant, but Oweolbori was recessive, showing partial dominance with high additive variance. Reno for grain-filling period (short grain-filling period) was dominant, but Oweolbori (long grain-filling period) was recessive with additive, and partial dominance. There were highly significant mean squares for both GCA and SCA effects on the heading and maturing times, and GCA/SCA ratios for all traits were high, showing the additive gene effects more important. Sacheon 6 and Oweolbori had greater GCA effects for early heading and maturing times, and Dongbori 1 and Reno had greater GCA effects for late times. GCA effects were highly significant in $F_1$ and $F_2$ generations, showing high GCA/SCA ratios (7.02). The heading and maturing times in field were positively correlated with antifreeze proteins concentrations, accumulation, resistance to photoinhibition and winter survival, respectively) but the grain-filling period did negatively correlated with the trails.
Data from five Hanwoo breeding farms were collected to analyze the effects of parity, season, nutrition and reproduction on body conditions of heifers and cows and to analyze the relationships of body conditions with reproductive performances and body growth. Analytic models included farms and parity which were run through SAS to estimate least squares means and correlation coeflicients between traits body condition scores(BCS) of I(very emaciated) through 9(very obese) scale at service and at calving. calving interval, days to rebreeding, gestation length. number of services per conception. Overall averages were 4.55 for BCS at service, 5.42 for BCS at calving. 406.7days of calving interval, 287.7days of gestation length, 66.2days from calving 10 first service and 1.78 times of services per conception. Number of services per conception was 1.50${\sim}$1.74 times for the cows of BCS 5 or under and 2.00${\sim}$3.00 for those of BCS 6 or higher. Body weight of calves from cows observed averaged 23.3kg at birth and 70.7kg at weaning. 57 percent of cows showed BCS 4${\sim}$5 at service while 46 percent of those showed 5${\sim}$6 at calving. Estimated phenotypic correlation coefficients of BCS at service with BCS at calving, calving interval, gestation length, number of services per conception were low but positive: 0.16, 0.26. 0.08 and 0.06. respectively. Phenotypic correlations of BCS at calving with calving interval, gestation length and number of services per conception were estimated to be also low and positive: 0.10, 0.13 and 0.10. respectively. However, phenotypic correlations between calving interval and gestation length, and between gestation length and number of services per conception were negative but low as - 0.11 and- 0.13 each.
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