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http://dx.doi.org/10.7235/hort.2015.14183

A Simple Method for Evaluation of Pepper Powder Color Using Vis/NIR Hyperspectral System  

Han, Koeun (Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University)
Lee, Hoonsoo (Department of Biosystems Machinery Engineering, Chungnam National University)
Kang, Jin-Ho (Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University)
Choi, Eunah (Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University)
Oh, Se-Jeong (Hana Seed Ltd.)
Lee, Yong-Jik (Hana Seed Ltd.)
Cho, Byoung-Kwan (Department of Biosystems Machinery Engineering, Chungnam National University)
Kang, Byoung-Cheorl (Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
Horticultural Science & Technology / v.33, no.3, 2015 , pp. 403-408 More about this Journal
Abstract
Color is one of the quality determining factors for pepper powder. To measure the color of pepper powder, several methods including high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and ASTA-20 have been used. Among the methods, the ASTA-20 method is most widely used for color measurement of a large number of samples because of its simplicity and accuracy. However it requires time consuming preprocessing steps and generates chemical waste containing acetone. As an alternative, we developed a fast and simple method based on a visible/near infrared (Vis/NIR) hyperspectral method to measure the color of pepper powder. To evaluate correlation between the ASTA-20 and the visible/near infrared (Vis/NIR) hyperspectral methods, we first measured the color of a total of 488 pepper powder samples using the two methods. Then, a partial least squares (PLS) model was postulated using the color values of randomly selected 3 66 samples to predict ASTA values of unknown samples. When the ASTA values predicted by the PLS model were compared with those of the ASTA-20 method for 122 samples not used for model development, there was very high correlation between two methods ($R^2=0.88$) demonstrating reliability of Vis/NIR hyperspectral method. We believe that this simple and fast method is suitable for highthroughput screening of a large number of samples because this method does not require preprocessing steps required for the ASTA-20 method, and takes less than 30 min to measure the color of pepper powder.
Keywords
ASTA; breeding; carotenoids; pepper fruit color; Vis/NIR hyperspectral system;
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1 Ahn, C.K., B.K. Cho, C.Y. Mo, and M.S. Kim. 2012. Study on development of non-destructive measurement technique for viability of lettuce seed (Lactuca sativa L) using hyperspectral reflectance imaging. J. Kor. Soc. Nondestruc. Test. 32:518-525.   DOI
2 Fox, G. and M. Manley. 2014. Applications of single kernel conventional and hyperspectral imaging near infrared spectroscopy in cereals. J. Sci. Food Agr. 94:174-179.   DOI
3 Gowen, A., C. Odonnell, P. Cullen, G. Downey, and J. Frias. 2007. Hyperspectral imaging - an emerging process analytical tool for food quality and safety control. Trends Food Sci. Technol. 18:590-598.   DOI
4 Hernandez-Ortega, M., A. Ortiz-Moreno, M.D. Hernandez-Navarro, G. Chamorro-Cevallos, L. Dorantes-Alvarez, and H. Necoechea-Mondragon. 2012. Antioxidant, antinociceptive, and antiinflammatory effects of carotenoids extracted from dried pepper (Capsicum annuum L.). J. Biomed. Biotechnol. 2012:524019.
5 Kim, S., J.B. Park, and I.K. Hwang. 2002. Quality attributes of various varieties of Korean red pepper powders (Capsicum annuum L.) and color stability during sunlight exposure. Food Chem. Toxicol. 67:2957-2961.
6 Kim, S., T.Y. Ha, and J. Park. 2008. Characteristics of pigment composition and colour value by the difference of harvesting times in Korean red pepper varieties (Capsicum annuum L.). Int. J. Food Sci. Technol. 43:915-920.   DOI
7 Lee, J.H., T.H. Sung, K.T. Lee, and M.R. Kim. 2004. Effect of gamma-irradiation on color, pungency, and volatiles of Korean red pepper powder. J. Food Sci. 69:585-592.   DOI
8 Lee, J.J., K.M. Crosby, L.M. Pike, K.S. Yoo, and D.I. Leskovar. 2005. Impact of genetic and environmental variation on development of flavonoids and carotenoids in pepper (Capsicum spp.). Sci. Hortic. 106:341-352.   DOI
9 Minguez-Mosquera, M.I. and D. Hornero-Mendez. 1993. Separation and quantification of the carotenoid pigments in red peppers (Capsicum annuum L.), paprika, and oleoresin by reversedphase HPLC. J. Agr. Food Chem. 41:1616-1620.   DOI
10 Minguez-Mosquera, M.I., M. Jaren-Galan, and J. Garrido-Fernandez. 1992. Color quality in paprika. J. Agr. Food Chem. 40:2384-2388.   DOI
11 Mo, H.S., K.S. Jang, J.E. Hwang, S.G. Jeon, and B.S. Kim. 2015. Horticultural and chemical quality characterization of accessions selected from four species of Capsicum. Hort. Environ. Biotechnol 56:54-66.   DOI
12 Rodriguez-Uribe, L., I. Guzman, W. Rajapakse, R.D. Richins, and M.A. O'Connell. 2012. Carotenoid accumulation in orangepigmented Capsicum annuum fruit, regulated at multiple levels. J. Exp. Bot. 63:517-526.   DOI
13 Tepic, A.N., and B.L. Vujicic. 2004. Colour change in pepper (Capsicum annuum) during storage. Acta Period. Technol. 35:59-64.
14 Wold, S., M. Sjostrom, and L. Eriksson. 2001. PLS-regression: a basic tool of chemometrics. Chemom. Intell. Lab. Syst. 58:109-130.   DOI
15 Wahyuni, Y., A.R. Ballester, E. Sudarmonowati, R.J. Bino, and A.G. Bovy. 2011. Metabolite biodiversity in pepper (Capsicum) fruits of thirty-two diverse accessions: variation in health-related compounds and implications for breeding. Phytochemistry 72:1358-1370.   DOI