• The Plant Pathology Journal
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• 제16권5호
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• pp.269-279
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• 2000

Alfalfa mosaic alfamoviruses(AIMV) were isolated from infected potato (Solanum tuberosum) and azuki bean (Paseolus angularis) in Korea. Two AIMV isolated from potatoes were named as strain KR (AIMV-KR1 and KR2) and AIMV isolated from azuki bean was named as strain Az (AIMV-Az). Each isolated AIMV strain was characterized by using their host ranges, symptom developments, serological relations and nucleotide sequence analysis of coat protein (CP) gene. Strains KR1, KR2, and Az were readily transmitted to 20 of 22 inoculated plant species including bean, cowpea, tomato, tobacco, and potato. AIMV-KR1 and KR2 produced the typical symptoms like chlorotic or necrotic spots in Chenopodium quinoa and Solanum tuberosum cv. Superior. AIMV-Az caused bright yellow mosaic symptom and leaf malformation in Nicotiana glauca, which were different from the common mosaic symptom caused by AIMV-KR1 and KR2. Electron microscope observation of purified virus showed bacilliform virions containing a single-stranded plus-strand RNAs of 3.6, 2.6, 2.0 and 0.9 kbp in length, respectively, similar in size and appearance to those of Alfamovirus. In SDS-PAGE, the coat protein of the two viruses formed a consistent band that estimated to be about 24kDa. The CP genes of the AIMV strains, KR1, KR2, and Az have been amplified by RT-PCR using the specific primers designed to amplify CP gene from viral RNA-3, cloned and sequenced. Computer aided analysis of the amplified cDNA fragment sequence revealed the presence of a single open reading frame capable of encoding 221 amino acids. The nucleotide and peptide sequence of viral CP gene showed that strain KR1, KR2, and Az shared highest nucleotide sequence identities with AIMV strain 425-M at 97.7%, 98.2%, and 97.2%, respectively. CP gene sequences of two strains were almost identical compared with each other. Altogether, physical, serological, biological and molecular properties of the purified virus.

• 식물병연구
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• 제17권1호
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• pp.59-65
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• 2011

토마토반점위조바이러스(Tomato spotted wilt virus, TSWV)에 대한 고추 유전자원 및 시판품종 등 100점의 병징 유형과 저항성 반응을 조사하였다. 병징 유형과 TSWV 감염률은 접종 후 9, 12, 14, 45일에 각각 육안으로 조사하였으며, 접종 14일에는 효소면역항체법을 이용하여 실험에 사용한 모든 개체의 비 접종 상엽에서 바이러스 감염 유무를 검정하였다. 감염된 대다수의 개체에서 잎 말림 증상이 가장 빈번하게 관찰되었으며, 국내 상용 $F_1$ 품종과 재래종고추에서는 황화 증상이, 야생형 수집종에서 줄기괴사 증상이 특이적으로 관찰되었다. TSWV의 전형적인 병징으로 알려진 원형반점은 조사한 일부 계통에서 관찰되었고, 계통 내 개체간 바이러스 병징 발현 정도는 상이하였다. 상용 F1 4품종(Kpc-35, -36, -57, -62)과 야생형 고추 8계통(PBI-11, C00105, PBC076, PBC280, PBC426, PBC495, PBC537, PI201238)은 유묘기에 기계적 접종했을 때 TSWV에 대해 고도의 저항성을 보였다.

• 한국응용곤충학회지
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• 제19권3호
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• pp.135-140
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• 1980

참깨종자의 종자전염균을 조사아던중, 우리나라에서는 미기록인 Macrophomina phaseolina가 12개 sample중 7개 sample에서 검출되었다. 본 균의 종자 종자상에서의 생육상 및 병자각과 병포자의 형태를 기술하였다. 본 균의 병자각형성에는 광선이 필순조건이었으나 PDA나 OA배지상에서는 병자각이 형성되지 않았고 물한천잎배지상에서 병자각이 많이 형성되었다. 본 균은 참깨종자의 심한 발아저해와 입고가 원인이 되었고 감자괴경에 탄부병징을 나타내었다. 목 균은 월동후의 참깨식물의 잔재와 포장의 이병유균에서도 검출되었다.

• 한국환경농학회지
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• 제15권2호
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• pp.207-216
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• 1996

인공산성비의 pH가 낮아질수록 고추씨의 발아에 걸리는 시간은 단축되었으며 발아율은 향상되었으나 어린 싹에게는 매우 치명적인 영향을 미쳤다. 어린 고추모는 이미 자란 식물체에 비하여 산성비에 훨신 더 민감하게 작용하였다. 산성비의 전형적인 가시피해증상은 잎에 나타나는 흰색 반점이었으며, 빗물의 pH가 낮을수록 더 빨리 더 심하게 나타났다. 고추의 역병은 일반적으로 인공산성비 처리구에서 대조구보다 빨리 발병하였으나, 인공산성비의 pH가 아주 낮을 때는 다른 처리구에 비하여 오히려 병의 발생이 줄어들었다. 인공산성비 처리중에 역병을 접종하였을 때는 처리 전에 접종하였을 때보다 병이 더 빨리, 더 심하게 나타났다. 역병의 발생이 가장 심했던 것은 역병의 접종시기와는 관계없이 pH 4.0 또는 4.5인 빗물을 처리하였을 때였다.

• 한국산림과학회지
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• 제67권1호
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• pp.28-30
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• 1984

형광색소(蛍光色素) DAPI(4'-6-diamidino-2-phenylindole, 2HCI)와 형광원미경(蛍光願微鏡)을 이용(利用)한 조직화학적(組織化學的) 기법(技法)으로 빗자루병(病)에 걸린 대추나무 조직내(組織內)의 마이코플라스마 분포(分布)를 조사(調査)한 결과(結果), 빗자루병징(病徵)이 나타나 있는 가지에서는 외관상(外觀上) 건전엽(健全葉)을 포함(包含)하여 모든 잎과 줄기에서 마이코 플라스마가 검출(檢出)되었으나 병징(病徵)이 나타나 있지 않은 가지의 잎과 줄기에서는 마이코플라스마가 검출(檢出)되지 않았다. 또한 감염(感染)된 나무의 뿌리조직(組織)에서도 뚜렷한 형광반응(蛍光反應)이 나타남으로써 마이코플라스마가 뿌리조직(組織)에도 많이 존재하고 있음을 알 수 있다.

• Journal of Nutrition and Health
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• 제21권2호
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• pp.81-87
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• 1988

This study attempted to investigate the relationships between vegetable preference and physical status. The subjects were 127 kindergarten children living Kwang-ju. In this study, vegetable preference and health status of the children were investigated by response of thier mothers through questionnaires. Also, rate of dental caries and anthropometric measures were obtained by examination. The results are summarized as follows; 1) Anthropometric mesures of the subjects were higher than those of Korean standards for children. 2) The mean hedonic score for vegetable preference in subjects was 2.6. The difference in vegetable preference between male and femal was not significant except for a squash item. 3) Vegetable prefernce has a significant correlation with the def. tooth rate. Especially preference of spinach, wild sesame leaf, and cucumber were negatively related to the def. tooth rate. 4) Vegetable preference and anthropometric measures did not show any significant correlation. 5) Children who ate sweets for snacks frequently disliked vegetable and showed high rate of dental caries significantly(p<0.001). Children who had anorexia and constipation disliked vegetables significantly (p<0.01). Children who complained diarrhea symptom showed significant high rte of dental caries(p<0.01). From the results above, as the negative relationship between vegetable preference and def. tooth rate was found, many cooking methods of vegetables should be studied and nutrition education should be oriented to recommend an increased intake of vegetables by children.

• 한국연초학회지
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• 제7권1호
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• pp.7-13
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• 1985

Effect of the two PVY strains(PVY-VB and PVY-VN) on yield and chemical constituents of tobacco plants was determined in roar. NC2326 and Burley 21. The virus was inoculated 6 and 8 weeks after transplanting, respectively. The strains and time of inoculation were most critical factors for yield reduction. The greatest reduction was caused by the necrotic strain (PVY-VN) inoculated 6 weeks after transplanting, accounting for the loss of 40% in var. NC2326 and of 45% in var.. Burley21. When inoculated 8 weeks after transplanting with the necrotic strain, only 17% reduction was recorded in roar. Burley21, but no reduction occurred in roar. NC2326. Generally less reduction was caused by the PVY-VB strain, ranging 12-16% depending on the time of inoculation and/or variety. Compared to healthy tobacco, PVY infected tobacco contained higher concentrations of total nitrogen, protein nitrogen, nitrate nitrogen, and lower total sugar in cured leaf of roar. NC2326. Total alkaloid, P, Mg, Ca, and K levels were not altered. In var. Burley21, protein nitrogen and nitrate nitrogen increased, but other chemical components were not changed. Necrotic strain-infected tobacco with a severe symptom had higher nitrogen than did mild strain-infected tobacco.

• The Plant Pathology Journal
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• 제21권4호
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• pp.383-386
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• 2005

In 2003 typical phytoplasma symptoms of witches' broom and flower malformation were observed on statice (Limonium sinuatum) plants grown at commercial greenhouses in Busan, South Korea. The DNA extracted from the infected leaves was amplified using universal primer pair of Pl/P6 derived from conserved 16S rRNA gene of Mollicutes giving the expected Polymerase chain reaction (PCR) product of 1.5 kb. In the nested PCR assays, the expected DNA fragment of 1.1 kb was amplified with the specific primer pair 16Fl/Rl that was designed on the basis of aster yellows (AY) phytoplasma 16S rDNA sequences. The 1.1 kb PCR products were cloned and nucleotide sequences were determined. The sequences were identical to that of Onion yellows OY phytoplasma (GenBank accession no. D12569) isolated from Onion in Japan. Electron microscopy of thin sections of leaf veins showed phytoplasma bodies in the phloem. Statice witches' broom symptom occurred on statice in commercial greenhouses in Korea was confirmed as infection of AY phytoplasma by transmission electron microscopy observation, and by determination of 16S rRNA gene sequences of phytoplasma.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.143.3-144
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• 2003

A Korean isolate of Pepper mild mottle virus (PMMoV-Kr) was isolated from a diseased pepper crop in Chunchon, Korea. The isolate was biologically purified on Nicoticaa tabacum cv. Xanthi-nc by successive single local transfer steps, and propagated on N. tabacum cv. Samsun. PMMoV-Kr could systemically infect on N. glauca, N. benthmiana, N. occidentalis and Lycopersicon esculentum, which is typical of known isolates of PMMoV. PMMoV-Kr belongs to the pathotype P1,2 based on pepper-tobamoviral indicator experiments; Capsicn chinone harboring L3 gene revealed resistant (necrotic local lesion on inoculated leaf, HR) whereas L+, L1 and L2 pepper plants expressed susceptible reactions of mosaic systemic symptoms for the isolate. To confirm the pathology and delineate symptom determinant of the isolate, full-length cDNAs of PMMoV-Kr were amplified by RT-PCR with a primer set corresponding to the 5'- and 3'-ends of PMMoV. The RT-PCR molecules amplified from genome RNA of the isolate was cloned into the pUC18 vector. Full-length cDNA clones constructed under the control of the T7 RNA promoter could be successfully transcribed to produce in vitro transcript RNA. Infectivity of the capped transcripts and its progeny virus was verified by Western blot and RT-PCR analyses.

• 인간식물환경학회지
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• 제22권3호
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• pp.233-239
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• 2019

This study was conducted in order to predict the vase life of cut lily 'Woori Tower' flowers using a non-destructive thermal imaging technique. It was found that the temperature of cut lily flowers was maintained at 20℃ and was slightly lower than the air temperature until they bloomed. On the 11th day, when flowers bloomed, the temperature of leaves and flowers was measured to be 18.75±0.38℃ and 19.23±0.32℃ respectively, and their difference with ambient temperature was over 3℃. The flower temperature increased slightly when the vase life of cut lily flowers ended, and the temperature difference between the air and leaf temperature (1.77℃) and between the air and flower temperature (1.39℃) got smaller. No visible aging symptom was observed, but it was found that the temperature had risen due to water losses and less functional stomata. The vase life of cut lily flowers can be predicted based on changes in temperature and it will be also possible to predict the potential quality and vase life of cut flowers before harvesting them in greenhouses.