• Journal of Ginseng Research
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• 제19권3호
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• pp.275-280
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• 1995

The formation of thylakoid membrane proteins and changes in the chloroplast ultrastructure of ginseng leaf were investigated as a function of time following the leaf emergence. The leaf chloroplast obtained just after the leaf emergence showed short rod-like thylakoids which were connected and arranged in 3~4 layers along the longitudinal axis of the chloroplast. The 10 DAE (days after emergence) chloroplast started to form grana structure. The typical grana structure was observed 17 DAE, and the grana was fully developed 28 DAE. The membrane proteins obtained from just after emerging leaf were separated into many minor bands indicating no CP-complex formation yet. LHC II was detected after 10 days. CP 47 and CP 43 were detected after 17 days. After 28 days, the PS I and PS II proteins were distinctly separated into CP 1, LHC II, CP 47, CP 43, CP 29, CP 27+24. Thus, the appearance of the light harvesting protein, LHC II, which was concentrated in grana stacks, was consis tent in time with the formation of grana stacks 17 DAE. Key words Chloroplast ultrastructure, grana, CP-complex, LHC II.

• 한국육종학회지
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• 제43권4호
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• pp.260-264
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• 2011

Leaf structure is one of the important agronomic traits. A rolled leaf mutant was induced from an ethyl methane sulfonate (EMS)-treated japonica rice, 'Koshihikari'. The rolled leaf mutant showed phenotypes of reduced leaf width and leaf rolling. In addition, several abnormal morphological characteristics were observed, including dwarfism, defected panicle, delayed germination, and lower seed-setting. Microscopic analysis revealed that the number of small veins was decreased and the sizes of adaxial bulliform cells were reduced in the mutant leaves. The genetic study with two $F_2$ populations from the crosses of the rolled leaf mutant with 'Koshihikari' and Milyang23 suggested that the mutant phenotype might be controlled by a single dominant gene.

• The Plant Pathology Journal
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• 제37권4호
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• pp.329-338
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• 2021

Alternaria leaf blight is one of the most common diseases in watermelon worldwide. In Korea, however, the Alternaria species causing the watermelon leaf blight have not been investigated thoroughly. A total of 16 Alternaria isolates was recovered from diseased watermelon leaves with leaf blight symptoms, which were collected from 14 fields in Korea. Analysis of internal transcribed spacer (ITS) region, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and RNA polymerase II second largest subunit (RPB2) were not competent to differentiate the Alternaria isolates. On the contrary, analysis of amplicon size of the histone H3 (HIS3) gene successfully differentiated the isolates into three Alternaria subgroups, and further sequence analysis of them identified three Alternaria spp. Alternaria tenuissima, A. gaisen, and A. alternata. Representative Alternaria isolates from three species induced dark brown leaf spot lesions on detached watermelon leaves, indicating that A. tenuissima, A. gaisen, and A. alternata are all causal agents of Alternaria leaf blight. Our results indicate that the Alternaria species associated watermelon leaf blight in Korea is more complex than reported previously. This is the first report regarding the population structure of Alternaria species causing watermelon leaf blight in Korea.

• 한국전산구조공학회논문집
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• 제31권5호
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• pp.243-250
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• 2018

본 논문에서는 복합재 판 스프링의 설계 최적화를 위해 유전자 알고리즘을 사용한 적층 최적화 과정을 제시하였다. 다목적 소형 승합 자동차 판 스프링을 유한요소모델로 구성하여 초기 설계를 검증한 이후, 유전자 알고리즘을 통해 복합재료의 적층수와 적층각도를 최적화하는 과정을 기술하였다. 최적화 과정을 통해 판 스프링의 하중 감소과정, 반복수에 따라 강 구조의 해석 결과와 비교하였다. 더불어 유전자 알고리즘을 통해 최적화된 적층 시퀀스를 구조에 적용하여 구조의 건전성을 검증하기 위해 유한요소 모델로 구성하여 안전여유를 계산하였다. GA를 적용할 때, 복합재료 판 스프링의 적층 두께와 적층 각을 획득하였으며, 이는 적절한 강도와 강성으로 최소 무게를 달성하는데 기여한다. 동일한 설계 매개 변수 및 최적화 조건에서 강철된 판 스프링을 복합재 판 스프링으로 교체하면 65.6%의 중량이 감소한다.

• Applied Biological Chemistry
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• 제15권1호
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• pp.41-47
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• 1972

새로 육종된 다수성 품종인 IR 667-수원 213과 진흥을 사용하여 출수후 군락구조의 등적 변화를 질소영양과 관련하여 비교분석한 결과 다음과 같았다. 1. 군락주조 패턴 (출수기 건물중 수직분포)은 진흥에서는 수직형이고 IR 667에서는 수평형이었다. 2. 엽면적 밀도 (또는 무게)의 수직분포 패턴이 IR 667은 중앙부 우세형으로 진흥은 상부 우세형으로 분류되었다. 3. 군락구조 보존패턴과 엽면적 백분율 분포패턴은 엽면적밀도의수직분포패턴을 따랐다. 4. 군락보존력은 IR 667이 약하여 군락보존율이 낮으며 이는 엽의노화가 빠른것을 나타낸다. 5. 질소영양이 군락구조패턴에 거의 영향을 주지 않지만 질소의 느린공급(유황입힌요소)은 구조의 중앙부를 보존하여 상부우세형을 중앙부 우세형으로 변환 시키려는 경향을 보였으며 그것이 수량 증가의 원인이 된것같다. 6. 증앙부우세형과 상부우세형은 곡실생산에서의 상위엽의존형과 하위엽 의존형에 각기 잘 적합한것 같다.

• Plant Resources
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• 제8권2호
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• pp.145-154
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• 2005

The antimicrobial effect of each fraction after fractionation of an ethanol extract of leaf mustard was examined in terms of nucleic acid, chloroform, ethylacetate, and butanol. The ethylacetate fraction, which showed the strongest level of antimicrobial effect among the different ethanol extract fractions of leaf mustard, was isolated and purified using silica gel column chromatography and HPLC, respectively, to obtain a single antimicrobial substance called KLM-1. The antimicrobial effect of this substance was 10 times higher than that of the ethylacetate fraction. A further study is on the way to confirm the structure of the antimicrobial substance KLM-1 through LC/Mass and NMR.

• Journal of Photoscience
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• 제9권2호
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• pp.166-169
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• 2002

This paper is a part of the research into effects of increased UV-B (Ultraviolet-B, 280-320nm) upon the ecosystem of field wheat. Based on a 3-year project with intensified UV-B influencing the crop, observation and calculation were made of such factors and parameters as the morphology of a single wheat plant including its leaf area, leaf base angle and proportion of spacing between joints. The results show that the enhanced UV-B signifantly decreased wheat's SSLA, and the increased percentage of the lower LAI is associated with the change in leaf base angle and proportion of spacing between joints.

• Journal of Plant Biology
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• 제31권2호
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• pp.155-164
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• 1988

The rapid senescence of detached Chinese cabbage leaf discs in darkness is first manifested by a sharp rise in malondialdehyde content (indicated by distruption of membrane structure), then by a rise in peroxidase activity and a decrease in catalase activity, and ultimately by chlorophyll degradation. These changes in parameters besides the catalase activity during senescence were delayed by application of spermidine. Especially, 10-4M spermidine almost completely arrested chlorophyll degradation after incubaton over 5 days. Spermidine reduced the amount of ethylene produced by senescing leaf discs. Additionally, it also reduced IAA-induced ethylene production. Calcium ion (1mM, 10mM) supplied together with the spermidine diminished the spermidine action, indicating probable involvement of an initial ionic attachment mechanism. These results suggest that spermidine can be used as an anti-senescence agent for plants and that this agent may stabilize membrane structure through interaction with the negatively charged loci on the membrane and exert the influence during senescence.

• 한국군사과학기술학회지
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• 제18권4호
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• pp.343-349
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• 2015

This is a study for the improvement of the fractured lug structure that connects the landing gear to the fuselage of the aircraft using the composite leaf spring landing gear. The lug surface was analyzed to find out the cause of fracture. The lug was destroyed by the crack initiation and propagation under the repeated stresses. The frictional wears of the lug structure were proceeded and that affected adversely to the crack. Also, the square protrusion of the lug has a weak shape to bring about stress concentration. The design changes were conducted and the test was performed to verify changed design results.

• Journal of the Korean Wood Science and Technology
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• 제35권2호
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• pp.9-15
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• 2007

A comparative study of the structure and organization of the primary and secondary walls in different types of tropical plant waste fibers was carried out using transmission electron microscopy (TEM). The thickness of each layer was also measured using Image Analyzer. TEM micrographs haveconfirmed that cell wall structure of all six types of tropical plant waste fibers (empty fruit bunch, oil palm frond, oil palm trunk, coir, banana stem and pineapple leaf) has the same ultrastructure with wood fibre. The fibers consisted of middle lamella, primary and thick secondary wall with different thickness for different types of fibers. The secondary wall was differentiated into a $S_1$ layer, a unique multi-lamellae $S_2$ layer, and $S_3$ layer.