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http://dx.doi.org/10.5423/PPJ.OA.02.2021.0018

Incidence of Alternaria Species Associated with Watermelon Leaf Blight in Korea  

Kwon, Oh-Kyu (Department of Molecular Biology, Sejong University)
Jeong, A-Ram (Department of Molecular Biology, Sejong University)
Jeong, Yong-Jik (Department of Bioresources Engineering, Sejong University)
Kim, Young-Ah (Department of Bioresources Engineering, Sejong University)
Shim, Jaekyung (Department of Molecular Biology, Sejong University)
Jang, Yoon Jeong (Department of Plant Science and Technology, Chung-Ang University)
Lee, Gung Pyo (Department of Plant Science and Technology, Chung-Ang University)
Park, Chang-Jin (Department of Molecular Biology, Sejong University)
Publication Information
The Plant Pathology Journal / v.37, no.4, 2021 , pp. 329-338 More about this Journal
Abstract
Alternaria leaf blight is one of the most common diseases in watermelon worldwide. In Korea, however, the Alternaria species causing the watermelon leaf blight have not been investigated thoroughly. A total of 16 Alternaria isolates was recovered from diseased watermelon leaves with leaf blight symptoms, which were collected from 14 fields in Korea. Analysis of internal transcribed spacer (ITS) region, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and RNA polymerase II second largest subunit (RPB2) were not competent to differentiate the Alternaria isolates. On the contrary, analysis of amplicon size of the histone H3 (HIS3) gene successfully differentiated the isolates into three Alternaria subgroups, and further sequence analysis of them identified three Alternaria spp. Alternaria tenuissima, A. gaisen, and A. alternata. Representative Alternaria isolates from three species induced dark brown leaf spot lesions on detached watermelon leaves, indicating that A. tenuissima, A. gaisen, and A. alternata are all causal agents of Alternaria leaf blight. Our results indicate that the Alternaria species associated watermelon leaf blight in Korea is more complex than reported previously. This is the first report regarding the population structure of Alternaria species causing watermelon leaf blight in Korea.
Keywords
Alternaria; disease; HIS3; leaf blight; watermelon;
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