• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.408-415
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• 2013

To better understand the morphological variation of the Perilla crop and their weedy types in East and Southeast Asia, we studied the morphological variation of 90 accessions by examining 10 morphological characteristics, such as flowering time, seed size, seed hardness, seed color, color of surface leaf, color of reverse side leaf etc. As a result, morphological variation determined that between cultivated var. frutescens and var. crispa, and between cultivated var. frutescens and its weedy type showed significant morphological differences in terms of seed size and seed hardness, whenever cultivated var. crispa and its weedy type could not showed significant differences in most morphological characters. In PCAs (principal component analysis), among 10 morphological characteristics, flower color (QL6), color of surface leaf (QL3), seed size (QN2), seed hardness (QL1), seed color (QL2), stem color (QL7), and color of reverse side leaf (QL4) contributed in negative direction on the first axis, while flowering time (QN1), leaf shape (QL5), and degree of pubescence (QL8) contributed in positive direction on the first axis. Among these morphological characters, particularly flower color (QL6), color of surface leaf (QL3), seed size (QN2), seed hardness (QL1), and degree of pubescence (QL8) were useful characters for discrimination between cultivated var. frutescens and weedy var. crispa, and between cultivated var. frutescens and its weedy type. However, most accession of cultivated and weedy types of var. crispa was not clearly discriminated by PCA analyses. Although the wild ancestral species of var. frutescens and of var. crispa are still unknown in East and Southeast Asia, the weedy types of Perilla crop may be the key taxon for our understanding of the origin of cultivated types of var. frutescens and var. crispa.

• Korean Journal of Environment and Ecology
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• v.23 no.5
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• pp.431-438
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• 2009

The main purpose of this research is to prepare and provide basic materials for the propagational strategy of eelgrass by investigating on the morphological adaptation of Korean Zostera marina to ocean currents. An eelgrass plant mainly consists of rhizome, leaf sheath, leaves and roots. The rhizome is the horizontal stem of the plant that serves as the backbone from which the leaves and roots emerge. The leaf sheath is the bundle at the base of the leaves that holds the leaves together, protecting the meristem, the primary growth point of the shoot. Leaves originate from a meristem which is protected by a sheath at the actively growing end of the rhizome. As the shoot grows, the rhizome elongates, moving across or within the sediment, forming roots as it progresses. The aggregated leaves from the leaf sheath are found to have two cell layers on one side and multiple layers of airy tissues called aerenchyma on the other. The aerenchyma tissues are developed in multi-layered cell structures surrounding the veins which are formed in the leaf sheath. Generative shoots are made of rhizomes, which are circular or ovoidal, stem, and spathe and spadix. The transverse section of rhizome and the stem and central floral axis is found to be circular, ovoid and in the shape of convex respectively, and the vascular bundle, which is a part of transport system, has one large tube in the center and two small tubes on both sides. The layers of collenchyma cells numbered from 12 to 15 in the stem, and from 7 to 12 in the rhizome. The seed coat is composed of sclereids, small bundles of sclerenchyma tissues, which prevent the influx of sea water from the outside and help endure the environmental stress. In conclusion, alternative multi-layer structure in circular, convex type aggregated leaf base are interpreted to morphological adaption as doing tolerable elastic structure through movement of seawater. The generative shoots develop long slim stem and branches in circular or ovoidal shapes to minimize the adverse impacts of sea current, which can be interpreted as the plant's morphological adaptation to its environment.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.292-297
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• 2015

A new cultivar of Hibiscus 'Red Bohanjae' (Hibiscus syriacus 'Red Bohanjae') was developed after crossing between Hibiscus syriacus 'Andong' (♀) with white flowers and Hibiscus syriacus 'Samchully' (♂) with pink flowers to improve flower quality and growth habit. The Hibiscus breeding program was initiated in 2002, and H. syriacus 'Red Bohanjae' was preliminarily selected as '02-RV-17' in 2003 due to its stable flower quality with rich pink flowers and small tree type. The selected line was further evaluated for growth characteristics, leaf shape, leaf size, flowering characteristics, and tested for distinctness, homogeneity, and stability during 2004-2007. The characteristics preserved by grafting. 'Red Bohanjae' showed a small growth habit with slightly upright branches. The tree had potential use as a potted plant or bonsai because of its dwarf form. The new cultivar 'Red Bohanjae' had violet-pink color flowers (RHS N78C) with red eye spots, small size (9.2 cm) and spatulate (I-type) petals. Leaves were 4.2 cm long and 2.3 cm wide. After the evaluation of plant characteristics for 3 years (2005-2007), it was registered as cultivar 'Red Bohanjae' (4118, plant variety protection rights number) in 2012. 'Red Bohanjae' having violet-pink flowers with red eye and dwarf tree form seems to be suitable for pot or bonsai cultivation in both indoor and outdoor conditions.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.711-714
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• 2010

A new cultivar 'Daemang' ($Hibiscus$ hybrid 'Daemang') originated from the interspecific cross between $Hibiscus$ $sinosyriacus$ 'Seobong' and $Hibiscus$ $syriacus$ 'Namwon' to improve the flower quality in 2001. 'Daemang' was preliminarily selected as 'R-143' in 2003 for its stable flower quality with long red eye spot and named in 2006. The tree habit shows vigorous growth and is upright, so it can be used as a specimen tree or street tree. Characteristic tests such as leaf shape, leaf size, flower characteristics, flowering, and capsule size were conducted from 2004 to 2006. The characteristics succeed after grafting. 'Daemang' had pink color flower with red eye spot. The width of flower is 15.2 cm. Petal length and width are 8.0 cm and 6.4 cm, respectively. Leaves are 9.49 cm long and 8.72 cm wide. After the plant characteristics evaluation for 3 years (2004~2006), it was registered as a variety 'Daemang' in 2008.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.171-176
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• 2008

Efficient plant regeneration system of birdsfoot trefoil (Lotus corniculatus L.) was development. The factors affecting the somatic embryo formation, its proliferation and regeneration capacity of leaf and stem explants of Empire cultivar was investigated. The highest number of somatic embryos was obtained on B5 medium supplemented with 1 mg/L NAA and 1 mg/L BA. Depending on different explants, highest frequency of embryogenic callus and regeneration were observed in Empire with leaf explants. The response from stem explants was slower and callus induction was less than that from leaf explants. Regenerated shoots formed complete plantlets in on 1/2 MS medium supplemented with 1 mg/L IBA. Regenerated plants were morphologically uniform with normal shape and growth pattern.

• Ocean and Polar Research
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• v.24 no.4
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• pp.345-357
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• 2002

Ecological characteristics on habitats and morphological features of the seagrass Zostera caulescens Miki in Korea were examined. The biogeographical distribution of Z. caulescens was confirmed in the south coast of the Korea. Zostera caulescens usually inhabits at the inner bay, where is sheltered from wave action and 3.0 to 6.5 m deep. The sediment in habitats is composed of very fine muddy sand or sandy mud. In morphology, Z. caulescens is easily distinguished from other species of the genus by the formation of vegetative canopy on the top of reproductive stems. The number of longitudinal ribs in testa was 24 revealed by scanning electron microscope (SEM) while the number of ribs in Z. marina has been often reported to be 16-20. Purplish anthocyanin spots were displayed on the surface when the testa was removed. We found that the size and shape of leaf epidermal cells in Z. caulescens were very different from those of Z. marina when the leaf cuticles were removed by maceration which could be useful characters for identifying Zostera species. The leaf of Z. caulescens displays two different casts of color when the fresh plant is closely observed. The margins of leaves appear brighter than the center of leaves due to the thickening by the development of paralleled venation in the middle of leaves. The comparison of two populations of Z. caulescens in Korea showed that they were considerably different in their shoot density and biomass. The shoot density and biomass at Gabae population was $367.3 m^{-2}\;and\;725.7g$ dry wt $m^P{-2}$ respectively, while those at Jukrim population were $112.5m^{-2}\;and\;392.0g\;dry\;wt\;m^{-2}$, respectively.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.447-456
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• 2013

This study was carried out for the establishment of theory and foundational data for breeding, on variegated foliage plants using for potted plants, based on examination and analysis for shape, color, and area ratio of variegated leaves and chlorophyll contents. Six families, 18 genus, and 54 species of variegated plants domestically distributed in Korea were used as the plant materials. Patterns based on chlorophyll-deficient part in variegated leaves were divided into 20 types, such as steps, border, collapsed border, sandy border, half, silk, stars, vein, firewood, border and firewood, center, entirety, leaf, irregular, net, watermelon, melon, and so on. There were 10 kinds of colors including dark green, red, dark red, reddish white, reddish yellow, white, silver, silvery white, yellowish white, and yellow. The ratio of variegated area was ranged from 5.8% to 100% and it was diverse depending on species or cultivar. The ratios by patterns were highest in entirety (98.6%) and relatively high in step (60.8%), whereas, low in boarder, star and firewood (33.6-36.4%), and relatively low in half and vein (43%). Chlorophyll content of variegated leaf was rather lower compared to normal plants and chlorophyll b tended to be higher in ratio of chlorophyll a to b. Particularly content of chlorophyll b in Stromanthe sanguinea 'Triostar', Dracaena fragrans 'Massangeana Compacta', D. reflexa 'Song of India', and Tradescantia spathacea was higher than chlorophyll a in comparison with that of normal plants.

• Journal of Conservation Science
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• v.33 no.5
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• pp.355-362
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• 2017

The Nongso tomb of Sunchang was built in a relatively deep place; hence there was little damage from grave robbers. The tomb was completely filled with a large amount of charcoal, and therefore the wooden coffin was excavated in relatively good condition. On the basis of the structure of the tombs, excavated artifacts, and radiocarbon dating results, the tomb is estimated to be from between the late Goryeo and the early Joseon period. The wooden coffin excavated is double-layered structures consisting of an outer coffin and an inner coffin. The outside of the wooden coffin is thickly lacquered and decorated with yellow letters and white motifs. An analysis of the pigments' components revealed that the major component of the letters was gold (Au) and the major component of the motifs was silver (Ag). The coffin lacquer had three layers: a pigment layer, a yellowish-brown layer, and a black layer. The wooden bases of the coffins were painted with a black substance, such as soot, as mineral elements were not detected in the black layer. The yellowish-brown layer is presumed to be refined lacquer. From the analysis of the structure of the layers and pigments, we can estimate the method was adopted for making lacquer for wooden coffin. It is assumed that the method used gold leaf and gold powder. The form of the pigment and the internal structure are likely to be gold leaf, but the shape of the surface letters appears to have been formed using gold powder. This study will serve as important information for understanding lacquer making techniques at the time of the tombs' construction by confirming the making method through reproduction experiments using gold leaf and gold powder.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.6
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• pp.1014-1019
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• 1994

To develope natural food preservatives, ethanol extract was preapred from the leaf mustard (Brassica juncea Coss) and antimicrobial activities were examined against 12 microorganisms which were food borne pathogens and/or food poisioning microorgaism and food-related bacteria and yeasts. The most active animicrobial concentration of the ethanol extract for most Gram positive microorganisms, Gram negative microorganisms, and lactic bacteria and yeasts was found to be 10, 20 and 40 mg/ml, respectively. when tested by a dose-response manner. Growth of Escherichia coli and STaphylococcus aureus were completely inhibited 4 hours after the addition of more than 20mg/ml of ethanole ethanol extract to the logarithic phase. Scanning electron icrographs of E. coli and Staph, aureus treated with ethanol extrract exhibited morphological changes, including the irregularly contracted cell surface of E. coli and expanded ellipsoidal shape of the Staph. aureus.

• Journal of Radiation Industry
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• v.6 no.2
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• pp.189-197
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• 2012

This study was carried out to develop the improved useful mutants for yield or composition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds of stevia 'Suwon No. 11' were irradiated up to 400 Gy of gamma ray. Chemical mutagens were treated on the seeds of the 'Suwon No. 11' using 0.07% colchicine, 10 mM sodium azide, or 10 mM NMU for various durations. The germination rate, and shoot and root growth of seedling were estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and the plant height, the number of branches, and leaf length and width were examined at 3 months after mutagenesis treatments. In the case of gamma ray treatments, the germination rate and early-stage growth were decreased as the increase of radiation dose, and the 50% lethal dose was found to be 200 Gy. the plant height was decreased as the increase of radiation dose, while the number of branches per plant and leaf length were increased. Leaf shape was modified to the relatively longer one compared to the control, which was identified more apparently at the treatments of higher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survival were decreased as the increase of incubation time. The 50% lethal dose for germination rate were identified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mM sodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemical mutagens had no influence on shoot growth, while root growth was increased, especially as the incubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrs incubation time. The plant height was decreased as the increase of incubation time in the chemical mutagens treatments. Among the chemical mutagens, NMU was the most effective to induce the mutants with long-shaped or the least lobed leaves.