• Molecules and Cells
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• v.28 no.2
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• pp.93-98
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• 2009

A plant-specific gene was cloned from melon fruit. This gene was named downward leaf curling (CmDLC) based on the phenotype of transgenic Arabidopsis plants overexpressing the gene. This expression level of this gene was especially upregulated during melon fruit enlargement. Overexpression of CmDLC in Arabidopsis resulted in dwarfism and narrow, epinastically curled leaves. These phenotypes were found to be caused by a reduction in cell number and cell size on the adaxial and abaxial sides of the epidermis, with a greater reduction on the abaxial side of the leaves. These phenotypic characteristics, combined with the more wavy morphology of epidermal cells in overexpression lines, indicate that CmDLC overexpression affects cell elongation and cell morphology. To investigate intracellular protein localization, a CmDLC-GFP fusion protein was made and expressed in onion epidermal cells. This protein was observed to be preferentially localized close to the cell membrane. Thus, we report here a new plant-specific gene that is localized to the cell membrane and that controls leaf cell number, size and morphology.

• Korean Journal of Plant Taxonomy
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• v.34 no.4
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• pp.297-319
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• 2004

Morphological characters of 14 taxa in 6 sections Ischnostachya(one taxon), Anomalae(one taxon), Capitellatae (five taxa), Debiles (one taxon), Capillares (one taxon) and Molliculae (five taxa) of genus Carex (Cyperaceae) were reexamined. The epidermal patterns of perigynium, achene and leaf were investigated using by using a scanning eletron microscope (SEM) and a light microscope (LM). Morphological characters such as rhizome, spike, width of leaf, leaf sheath, involucre, involucre sheath, beak of perigynium, perigynium shape, and leaf epidermal patterns were useful for the identification of observed fourteen taxa. The number of spike, arrangement of psitillate or staminate spike and presence of bract were very useful characters to divide the treated 14 taxa into two groups. A key based on data was presented here.

• Journal of People, Plants, and Environment
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• v.24 no.4
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• pp.377-387
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• 2021

Background and objective: To improve air quality, particulate matter (PM) can be reduced using green infrastructure. Therefore, in this study, we aimed to determine the particulate matter reduction potential of climbing plants used for green walls, an element of vertical green infrastructure. Methods: A sealed chamber with controlled environmental variables was used to assess the PM reduction level caused by climbing plants. PM concentration in the plant chamber was measured after two and four hours of PM exposure, and the reduction potential was assessed based on the leaf area. Results: Compared to the empty chamber (Control), the PM reduction speed per hour was higher in the plant chamber, which confirmed that climbing plants contribute to the reduction of PM in the air. The PM reduction speed immediately after exposure in the plant chamber was high, but this slowed over time. Additionally, PM has been continuously reduced in plants with large leaves. As a result of calculating the particulate matter reduction level based on leaf area, it was found that there was a difference by particle size. Actinidia arguta, Parthenocissus tricuspidata, Trachelospermum asiaticum, and Euonymus fortunei var. radicans showed a high reduction effect. The trichomes on the leaf surface of Trachelospermum asiaticum were found to affect PM reduction. Conclusion: PM adsorption on the leaf surface is an important factor in reducing its concentration. It was possible to compare different plants by quantifying the amount of PM reduction during a fixed time period. These results can be used as the basic data to select the plant species suitable for urban green walls in terms of PM reduction.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.163-187
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• 2006

Taxonomic studies were conducted to evaluate the interspecific relationships in Viola albida var. albida and its related taxa using seven populations for morphology, palynology and anatomy. Molecular phylogenetic studies were also examined in 28 populations including 19 Korean, four Chinese, two Japanese, one American population and two outgroups using nrITS, and 27 populations except V pinnata for trnL-F region of chloroplast DNA. Morphological differences was observed among seven populations of three species in leaf shape, but characters such as serrate number of leaf margins, petal size, pistil shape were showed overlap between populations. Pollen shape of seven populations was monad and grain shape on the polar axis was semi-angular. Morphology of aperture was tri-colporate, and the surface sculpturing was scabrate in rugulate. The grain shape of equatorial view of five populations was prolate whereas V albida var. taknhashii type 1 and V albida var. chaerophylloides type 3 were subprolate. The anatomical characters of rnidvein of leaf, petiole, peduncle, root were also described for the species. The stomatal apparatus of the leaves was observed only in abaxial surface, and the number of stoma per unit ($mm^2$) were abundant in incised than lobate or cleft margin of the leaves. The nrITS analysis shows that V. pinnata and V. dissecta was monophyletic and occupied a basal position in the V. albida var. albida and its related taxa. The other clade including infraspecific populations of V. albida, and V. eizanensis was paraphyletic. The trnL-F noncoding region analysis was similar to the ITS tree. According to the above observations in morphology, palynology, anatomy, and molecular phylogenetic analysis, the significant differences were not found except for leaf shape in Viola albida var. albida and its related taxa, therefore V. albida var. takanhashii and V. albida var. chaerophylloides were considered to be an infraspecific taxa of V. albida var. albida rather than an independent species, subvariety or variety of V. pinnata and V. dissecta.

• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.52-63
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• 1984

This study was intended to identify 17 taxa. (5 varieties and 12 species) of the genus Acer in Korea on the basis of the shapes of stomata, the type of trichome on the different part of leaves, the shapes, arrangement and number of stele in cross section of petiole. The results obtained were summarized as follows; 11 The shape of guard cells of stomata in the genus Acer was anomcytical, and the size of the cells ranged from 10.25 to $21.00{\mu}$ in length and from 7.57 to $11.83{\mu}$ in width. 2) Eleven types of trichome on the leaf in the genus Acer were found; pilose, sericeous, velutinous, woolly, glabrate, puberulent, bladder hair, hispid, hirsute and uncinate. This characteristics also established a good criterion for identification of species. 3) The stele of petiole in the genus Acer was characterized by eustele and atactostele with polybranch, and the six groups of the shape of numerical change of stele; B>M=T, BM

• Korean journal of applied entomology
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• v.12 no.3
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• pp.121-124
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• 1973

From the new rice variety Tong-il, disease symptoms similar to Fusarium leaf spot reported in Japan was observed in Korea. Causal organism was isolated and identified as Fusarium nivale causing Fusarium leaf spot through the study of conidia shape, ascus formation on diseased spot, and ascospore. These results also showed good agreement with that of other investigators. Under the field condition, marked occurrence of this disease has observed by heavy nitrogen application. The optimum temperature for the growth of this fungus was $24^{\circ}C$.

• Journal of Sericultural and Entomological Science
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• v.43 no.1
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• pp.9-15
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• 2001

New Mulberry Cultivar, Milsungppong, was selected from F1 seedlings obtained by crossing the female, 4X Keomseolppong, and the male, Kaeryangppong. Local adaptability test has been carried out at five places(Suwon, Chunchun, Cheongju, Kongju and Sangju) for four years since 1992. Leaf quality was tested by silkworm rearing for two silkworm rearing seasons(spring and autumn) at Suwon in 1995. From these tests, Milsungppong was found to be superior to control cultivar, cultivar, Kaeryangppong, in productivity, rootability and shoot cold injury. The new cultivar, Milsungppong, has following agronomic characteristics as compared with Kaeryangppong, the most prevailing cultivar, Milsungppong, has following agronomic characteristics as compared with Kaeryangppong, the most prevailing cultivar in Korea It is triploid cultivar belonging to Morus bombycis KOIDZ with the leaf shape of five lobes, sprouting in spring nearly the same to the Kaeryangppong (Morus alba L.), high leaf yielding of 25% compared to Kaeryangppong (Morus alba L.) and the medium degree of cold resistance. It is adaptable to every where in Korea except the area cold injury broken out habitually. Milsungppong is recommended to rear silkworms not only in spring but also in autumn besides for young silkworms.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1885-1889
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• 2007

The tissue blot immunobinding assay (TBIA) is widely used for the detection and localization of plant viruses in various plant tissues. The basic experimental procedures of TBIA sampling and blotting were simplified using commercially available micropipette tips. This method was termed the ring-blot immunobinding assay (R-BIA), as the blot on the membrane forms a ring shape. The detection efficacy of R-BIA was tested for two chili pepper viruses, pepper mild mottle tobamovirus (PMMoV) and pepper mottle potyvirus (PepMoV), following the optimized serological procedures of TBIA (length of the incubation period and BSA concentration, and primary and secondary antibodies). Sensitivity of the R-BIA was about 1 ng/ml of purified PMMoV in pepper leaf sap from a healthy pepper plant. R-BIA also showed high specificity in the detection of PMMoV and PepMoV. Moreover, the modified sampling and blotting procedures were simpler and more reliable than other TBIA methods (such as whole-leaf blotting and crushed-leaf blotting), suggesting that the R-BIA may be used for medium- to large-scale detection of plant viruses in laboratories with minimal facilities.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.415-419
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• 2014

A Cymbidium variety 'Snow Bell' (C. kanran ${\times}$ C. Jack Frost) was developed at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2011. A cross between C. kanran with small-sized greenish colored flowers and C. Jack Frost with bright pink colored flowers, was conducted in 1999. Based on flower color, leaf shape, flower stalk, and vigor one line was selected. Evaluation and selection trials were conducted from 2006 to 2010. 'Snow Bell' has a light pink sepal (RHS, R56D), red lip (R56A) and a leaf length of 47.5 cm. The average number of flowers on each peduncle is 13.8 and the flower diameter is 6.2 cm. The characteristics of petals and sepals show some incurve and some spreading. 'Snow Bell' has a vigorous growth and a round flower. It has an adequate peduncle height of 50.7 cm compared with leaf length of 47.5 cm, and start blooming in December. In electronic nose analysis, the degree of fragrance between 'Snow Bell' and the C. kanran was similar and both of them showed strong scents, although they had different fragrance patterns.

• Korean Journal of Plant Taxonomy
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• v.40 no.4
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• pp.251-254
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• 2010

We describe a new taxon of Goodyera (Orchidaceae), G. ${\times}$ tamnaensis N.S. Lee, K.S. Lee, S.H. Yeau & C.S. Lee, sp. nov., from Jeju Island. This taxon is presumed to be a hybrid between G. schlechtendaliana and G. velutina based on several morphological characteristics, i.e., leaf venation, a patterned leaf surface and raised leaf epidermal cells, the color of the flower and the bract, the lateral sepal shape, and the hair length. The morphological characteristics and illustrations of the species based on the holotype are provided together with photographs of the habitat.