• Food Science and Biotechnology
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• 제15권2호
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• pp.277-282
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• 2006

The aim of this study was to investigate the anti-inflammatory and anti-oxidant activity of Ligularia fischeri leaf extract on adjuvant induced arthritis in experimental mice. The oral administration of the L. fischeri leaf extract (LF), at doses of 100 and 200 mg/kg body weight once a day for 3 weeks, significantly reduced hindpaw swelling and the production of inflammatory cytokines (tumor necrosis factor(TNF)-${\alpha}$, interleukin(IL)-$1{\beta}$, and IL-6). Treatment with LF (100 mg/kg) also decreased the serum levels of triglyceride and low density lipoprotein(LDL)-cholesterol, and increased high density lipoprotein(HDL)-cholesterol contents compared with those of a control group. The induction of arthritis significantly increased oxidized proteins such as protein carbonyl, advanced oxidation protein products, and advanced glycation end-products in the lung, heart, and brain. Treatment with LF for 3 weeks reduced the levels of oxidized proteins. These results suggest that L. fischeri extract might be beneficial in the treatment of chronic inflammatory disorders.

• 생명과학회지
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• 제9권5호
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• pp.497-503
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• 1999

Change on the structural carbohydrate(several fiberous components) was determined by vegetables(crown daisy and butterbur)-cultivated in Ulsan, Kyungnam, Korea-as its stage of maturity developed. Samples were separated into leaf and stem, which were dried at 7$0^{\circ}C$ for 24hr, and ground to pass a 1mm screen. They were subjected to moisture, crude protein, crude fat and several dietary fiber-DF(dietary fiber, include unavaible components), NDF(neutral detergent fiber), ADF(acid detergent fiber), lignin, hemicellulose, cellulose and protein corrected NDF(c-NDF), IDF(indigestible fiber, include lignin, hemicellulose and cellulose). In general, structural carbohytrate(several dietary fiber) of vegetable was affected by the growth stage. In case of crown daisy and butterbur, dietary fiber in leaf was higher than DF in stem.

• 한국식품조리과학회지
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• 제17권1호
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• pp.80-90
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• 2001

This study was carried out to investigate to proximate compositions, free sugars, organic acids, amino acids, and volatiles from the fresh leaf, root and seed of coriander. The research results are as follows: Moisture was 79.93% in the leaf, 81.89% in the root. Crude protein, crude lipid and crude ash were the highest in the seed. Ascorbic acid was 65.4mg% in the leaf and 37.83mg% in the root. Glucose, fructose and sucrose were the major free sugars. Glucose was 7.92mg% and fructose 7.51mg% in the leaf. Sucrose was 17.34mg% in the root, highest level. Among organic acids, malic acid was 354.55mg% in the leaf, the highest level. The content rate of organic acids was high in the order of leaf, seed and root. The content rate of total amino acid was high in the order of seed, root and leaf. Glutamic acid and aspartic acid were high in the leaf and root. Glutamic acid and proline were high in the seed. The content rate of free amino acid is the same as that of total amino acid. Glutamic acid and serine were high in the leaf and seed. Glutamic acid and treonine were high in the root. The contents of total amino acid in each parts of the coriander was higher than that of free amino acid, The composition of amino acid in the total amino acid and free amino acid was different. The volatile constituents were extracted by steam distillation method and analyzed by GC-Mass. The content of the volatile constituents was 45.31mg% in the leaf, (E)-2-decenal was the highest, followed by decanal, 2-dodecenal, (E)-2-decen-1-ol in order, aldehyde and alcohol was major constituents. The content of the volatile constituents was 36.01mg% in the root and 54.37mg% in the seed. linalool was the highest in the root and seed. it was 22.27 %, 53.67% in root and seed.

• Journal of Plant Biology
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• 제35권2호
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• pp.117-123
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• 1992

암배양을 통한 노쇠중인 밀 제1엽에서 지질의 과산화반응과 불용성 잎단백질의조성 및 엽록체 틸라코이드 막단백질 조성의 변화에 대한 BA의 효과가 조사되었다. 성숙한 밀 제1엽을 잘라내어 4일간의 암배양을 통한 노쇠유도실험에서 $10^{-5}\;M$ Benzyladenine(BA)은 노쇠중인 밀잎에서 엽록소 함량 및 수용성과 불용성 단백질 함량의 감소를 크게 억제시켰다. 특히, 단배질 함량 감소에 대한 BA의 억제효과는 수용성 보다는 불용성 단백질에 있어서 더욱 현저하였다. 또한, BA로 처리된 잎에서 지질의 과산화물인 MDA 함량의 증가가 억제되었다. 불용성 단백질에 대한 SDS-전기영동 결과 양적으로 현저한 57, 26 및 12 KD 단백질이 다른 소량의 단백질 무리와 함께 분리되었다. 대조구 잎에서의 불용성단백질 조성의 변화는 72시간의 암배양동안 57 KD와 12 KD 단백질이 현저하게 분해 소실되었으나 26 KD 단백질은 비교적 분해가 덜 일어났으며 BA 처리시 이들 단백질의 소실이 크게 억제되었다. 엽록체 틸라코이드막 단백질 조성의 경우, 각각 CF의 $\alpha,\;\beta$ subunits인 59 KD 단백질과 57 KD 단백질 및 LHCP 단백질인 26 KD 단백질을 포함하는 20개 정도의 단백질이 SDS-전기영동상에서 분리되었다. 72시간의 암배양 동안 대조구 엽록체에서 이들 단백질들이 급속히 분해 소실되었으나 BA로 처리된 엽록체의 경우 이들 단백질의 분해가 정량적으로 크게 억제되었다. 위의 결과들은 BA가 노쇠중인 밀잎에서 막지질의 과산화반응 억제를 통해 막단백질의 손실을 지연시키며 그로 인하여 엽록체 틸라코이드막을 포함한 세포막이 유지될 수 있음을 나타내었다.

• Applied Biological Chemistry
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• 제29권1호
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• pp.10-15
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• 1986

Cytokinin과 단백질의 결합을 간단하게 결정하는 방법으로서 전기 영동법을 시도하고, 대두의 잎단백질과 cytokinin의 결합여부, cytokinin에 대한 affinity가 있는 단백질의 종류와 상대적 affinity를 조사하였다. 검토된 전기 영동법은 cytokinin과 단백질의 결합뿐만 아니라, cytokinin에 대한 상대적 affinity를 동시에 검정할 수 있는 장점을 가지고 있었다. Ammonium sulfate 침전법, Sephadex G-25 chromatography, paper chromatography, 그리고 전기영동법으로 대두의 잎단백질 중에 BA와 결합하는 단백질이 있음을 확인할 수 있었다. 전기영동법으로 검정한 결과 BA와 결함하는 것이 3 group이 있고, 이중에서 전기영동 이동도로보아 분자량이 작은 단백질 분획과 전기영동 이동도 0.4부근의 분획은 BA에 대한 affinity가 비교적 낮은 반면, 이동도 $0.0{\sim}0.2$의 분획은 affinity가 큰 것으로 생각되었다.

• 한국연초학회지
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• 제1권2호
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• pp.120-126
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• 1979

목포, 남원, 예산지방에서 각각 재배한 Burley 엽중의 nitroate form nitrogen, alkaloid form nitrogen, ammonia form nitrogen, protein form nitrogen과 form nitrogen의 함량을 조사하였다. Total nitrogen 중에서 protein form nitrogen이 차지하는 비율이 30-33%로써 가장 높았고, nitrate form nitrogen이 10∼23%, alkaloid form nitrogen이 8∼16%, ammonia form nitrogen이 6∼9%, 그리고 amide form nitrogen이 2∼2%였다. Nitrate form nitrogen의 함량 비율은 예산, 남원, 목포 순으로 증가하는데 반하여 alkaloid form nitrogen은 반대되는 현상을 보였다. 등급에 따라서는 alkaloid form nitrogen과 ammonia form nitrogen의 함량 비율의 순위는 H5>3>L1>3>5이였으며 nitrate form nitrogen은 이와 반대되는 경향이었다.

• 한국연초학회지
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• 제33권1호
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• pp.16-20
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• 2011

This study was carried out in order to improve the Mohr method for protein-nitrogen concentration determining. Existing Mohr's method takes seven hours to analyze for one sample. In order to make up for these things tried to utilize filter bag(F75, ANKOM) for sample treatment. As a result of this implementation, have saved sample loss amount and analyzing time for a quarter comparing with Mohr's method. In addition, it have gotten high efficiency through five samples hydrolyze per one trial by using ANKOM hydrolysis system with 0.5% acetic acid. Besides, had a good reproducibility for analysis results in relation to nitrogen concentration determining with Dumas methodology. Thus, new Mohr's method takes one day to analyze for 40 sample. It is more efficiency about 6 times compare with existing Mohr's method. And, this modified Mohr's method was verified that is substitutable for the existing Mohr's method in statistical analysis.

• Molecules and Cells
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• 제26권3호
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• pp.270-277
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• 2008

This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol. BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol. Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein. In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes. The molecular role of RanBP1 in plants is discussed in comparison with RanBP1 function in yeast and mammals.

• 통합자연과학논문집
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• 제1권1호
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• pp.19-23
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• 2008

Morus alba has been well known for its tonic effect or preventing of hypertension, aging, or diabetes mellitus in Asian countries. To evaluate the its effect on hair growth promotion, we performed the hair regrowth experiment with leaf, branch, root and fruit extract of Morus alba on animal model of C57BL/6 mouse. The hair regrowth effect was remarkable in the mouse treated branch and root extract (with what %), however, little effect on hair regrowth appeared on the mouse treated with leaf extract. Morus alba extract displayed antibacterial effects on Bacillus subtilis, Staphylococcus aureus, Pseudomonas and Candida albicans. These results indicated that branch and root extract of Morus alba could be applicable for hair regrowth or prevention of hair loss in human.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.273-282
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• 2020

In Vietnam, Celastrus hindsii Benth, a medicinal plant rich in secondary metabolites, has been used to alleviate distress caused by ulcers, tumors, and inflammation for generations. The occurrence of two phenotypes, Broad Leaf (BL) and Narrow Leaf (NL), has raised questions about the selection of appropriate varieties for conservation and crop improvement to enhance medicinal properties. This study examined molecular differences in C. hindsii by comparing protein profiles between the NL and BL types using 2D-PAGE and MS. Peptide sequences and proteins were identified by matching MS data against the MSPnr100 databases and verified using the MultiIdent tool on ExPASy and the Blast2GO software. Our results revealed notable variations in protein abundance between the NL and BL proteomes. Selected proteins were confidently identified from 12 protein spots, thereby highlighting the molecular variation between NL and BL proteomes. Upregulated proteins in BL were found to be associated with flavonoid and amino acid biosynthesis as well as nuclease metabolism, which probably attributed to the intraspecific variations. Several bioactive proteins identified in this study can have applications in cancer therapeutics. Therefore, the BL phenotype characterized by healthier external morphological features has higher levels of bioactive compounds and could be better suited for medicinal use.