Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
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Optimum Determination Method of Protein-Nitrogen Improve Mohr Method in Leaf Tobacco

잎담배 단백태 질소 분석법(Mohr법) 개선

  • Received : 2011.11.01
  • Accepted : 2011.11.21
  • Published : 2011.12.24
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Abstract

This study was carried out in order to improve the Mohr method for protein-nitrogen concentration determining. Existing Mohr's method takes seven hours to analyze for one sample. In order to make up for these things tried to utilize filter bag(F75, ANKOM) for sample treatment. As a result of this implementation, have saved sample loss amount and analyzing time for a quarter comparing with Mohr's method. In addition, it have gotten high efficiency through five samples hydrolyze per one trial by using ANKOM hydrolysis system with 0.5% acetic acid. Besides, had a good reproducibility for analysis results in relation to nitrogen concentration determining with Dumas methodology. Thus, new Mohr's method takes one day to analyze for 40 sample. It is more efficiency about 6 times compare with existing Mohr's method. And, this modified Mohr's method was verified that is substitutable for the existing Mohr's method in statistical analysis.

Keywords

References

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