• BMB Reports
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• v.50 no.7
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• pp.343-344
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• 2017

Plants are able to recognize even small changes in surrounding temperatures to optimize their growth and development. At warm temperatures, plants exhibit diverse architectural adjustments, including hypocotyl and petiole elongation, leaf hyponasty, and reduced stomatal density. However, it was previously unknown how such warm temperatures affected the early stages of seedling development. In our recent study, we demonstrated that the RNA-binding protein, FCA, is critical for sustaining chlorophyll biosynthesis during early seedling development, which is a prerequisite for autotrophic transition at warm temperatures. FCA plays a dual role in this thermal response. It inhibits the rapid degradation of protochlorophyllide oxidoreductases (PORs) that mediate chlorophyll biosynthesis. In addition, it induces the expression of POR genes at the chromatin level, which contributes to maintaining functional enzyme levels. Our findings provide molecular basis for the thermal adaptation of chlorophyll biosynthesis during the early stages of seedling development in nature.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.33-37
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• 2014

Lychnis wilfordii (Regel) Maxim is a rare and valued ornamental plant. Germination rate reached 46.6% when seeds were treated with $100mg{\cdot}l^{-1}$ GA (Gibberellic acid). The highest callus induction was observed in the leaf explants of the seedlings on MS medium containing specific concentrations of $0.5mg{\cdot}l^{-1}$ BA ($N^6$-benzyladenine) and $3.0mg{\cdot}l^{-1}$ NAA (a-naphthalene acetic acid). The adventitious shoot was formed in 97.3% of calli on 1/2 WPM (Woody Plant Medium) medium. Shoot elongation of in vitro propagated plantlets was no difference among various medium. The plantlets grew well after transferring to the pot. This in vitro propagation protocol should be useful for conservation of this endangered plant.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.399-403
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• 2021

Stem blight symptoms were observed in greenhouse-grown bottle gourds (Lagenaria siceraria) in Wanju, Korea. Infected plants showed leaf chlorosis and wilting; the plants eventually died with the infected leaves remaining attached, and black sclerotia formed on the diseased stem. Based on the morphological characteristics and pathogenicity results and sequence analyses of the internal transcribed spacer, translation elongation factor 1-α, β-tubulin, and calmodulin regions, the isolated fungus was Macrophomina phaseolina. This is the first report of charcoal rot in Lagenaria siceraria caused by Macrophomina phaseolina in Korea.

• Journal of Life Science
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• v.24 no.2
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• pp.148-153
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• 2014

The objective of this study was to elucidate the effect of light-emitting diode treatment on early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Oak Leaf'). In changes to leaf morphology, shoot elongation and hypocotyl length showed poor growth under red light irradiation, while red+blue light irradiation induced shorter plant height and more leaves, resulting in increased fresh weight. With respect to Hunter's color and SPAD values, lettuce seedlings grown under red+ blue and fluorescent light irradiation had a higher $a^*$ value but showed no other changes to SPAD values. Interestingly, redness in relative chlorophyll content was 1.4 times higher under red+blue light irradiation. Inorganic element (N, Ca, Mg, and Fe) and ascorbic acid concentrations increased in lettuce plants grown under LED light irradiation compared to those of lettuce grown under fluorescent light, which showed a higher P content. In conclusion, red+blue light irradiation, which stimulates growth and higher nutrient uptake in leaf lettuce, could be employed in containers equipped with LEDs.

• Journal of the Korean Society of Tobacco Science
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• v.7 no.1
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• pp.15-23
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• 1985

Growth response and evapotranspiration of tobacco as to each soil moisture condition treated with different height of ground water table such as 30cm, 100cm, 170cm, and 230cm were investigated. The results obtained were as follows; 1. Soil water potential in the rhizosphere (25cm depth from surface) treated with ground water table (G.W.T) of 230 cm was less than -6.5 bar at 60 days after transplanting. 2. Dry weight of leaves per plant was the highest in the pot treated with 100 cm G.W.T and was the lowest in the pot treated with 230 cm G.W.T.. Leaves/Stem ratio of dry weight was the lowest in the pot treated with 230 cm G.W.T. This would show that extension of leaves as compared with elongation of stem was suppressed. .3. In the roster stress condition of 230 cm G.W.T., extension of leaves was continued until the latter growth stage, Relative growth rate of leaf area per plant of the pot with 30 cm G.W.T. was the highest through all growth period after fixation of G.W.7., but owing to lower density of leaf, the dry weight of leaves per plant was less rather than that of the pot with 170 cm G.W.T. at 64 days after transplanting. 4. The highest evapotranspiration per plant per day was shown at the 45th day after transplanting in the pots with 30cm and 100cm G.W.T. and at 60 days in the pots with 170cm and 230cm G.W.T respectively. 5. In the soil moisture conditions with 30cm and 100cm G.W.T., evapotranspiration per plant per hour was much higher in the daytime that at night as well as in clear day than in rain-cloudy day owing to loner relative humidity , but in the water stress condition with 230 cm G.W.T., that was much lower. 6. Total evapotranspiration per plant during the whole growing period of 64 days was in order of 30cm > 100 cm > 170 cm > 230 cm G.W.T.. Total evapotranspiration for 34 days after fixation of G.W.T was much higher at night than in the daytime in case of water stress conditions i.e 170cm and 230cm G.W.T. but in normal condition of the pots with 30cm and 100cm G.W.T. that was much higher in the daytime than at night. 7. Leaf water potential of the 3rd and 6th leaf from top at 64 days after transplanting was in order of 100cm > 30 cm > 170cm > 230cm G.W.T. The reason why leaf water potential in the pot with 30cm was less than that in the pot with 100cm G.W.T. would be the abnormal turgidity with low density of cell substrates by the excess water absorption in the pot with 30cm G.W.T..

• Journal of Bio-Environment Control
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• v.29 no.4
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• pp.365-372
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• 2020

This study was conducted to compare the effects of foliar spray and sub-irrigation of the triazole fungicide diniconazole on the regulation of stem elongation and to investigate the stimulation of root system development during the seedling stage. Comparing the two application approaches, there were significant differences in the leaf area, leaf area ratio (LAR), plant height, compactness, fresh shoot and root production, relative growth rate (RGR), and root to shoot ratio (R/S). At the same application concentration, the sub-irrigation showed a better retarding effect on growth than the foliar spray, because the PGR activity of diniconazole in root absorption was higher than that in shoot absorption. For reaching a target of 20% to 30% inhibition rate of stem length, foliar application concentration of diniconazole exceeded 10, however, only approximately 1 was required in the sub-irrigation application. The root system of tomato seedlings responded strongly to diniconazole application. Total root length, root volume, root average diameter, and the number of root tips increased when diniconazole was sub-irrigation application at 1. A reduction in fine roots (diameter range of 0 to 0.3 mm) and an increase in the roots with a diameter range of 0.3 to 0.6 mm was observed, and this may contribute to the increase in average diameter. The increase in root average diameter may be positive because root penetration increases with root diameter. Our results suggested that sub-irrigation maximized the PGR activity of diniconazole to enhance the retarding effect. And it also possible to enhance the tomato seedling root system by diniconazole stimulating with a lower concentration.

• Journal of Plant Biotechnology
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• v.41 no.2
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• pp.100-106
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• 2014

In order to develop an efficient in vitro micropropagation technique for a rare plant species, Astragalus membranaceus Bunge var. alpinus N., shoot proliferation and in vitro or in vivo rootings were conducted and hyperhydrated leaf generated from cultures was histologically observed. During shoot induction, no distinct effect on multiple shoot induction was found between BA and kinetin treatment. BA enhanced the number of internodes, whereas kinetin stimulated shoot elongation. Hyperhydrated leaf composed of bigger cells and retarded palisade parenchyma and showed irregular cell arrangement compared to normal leaf. Especially starch content in hyperhydrated leaf was significantly reduced. The best rooting rate was achieved by B5 medium among three different medium (B5, MS and WPM) and 0.1mg/L IBA treatment induced the highest rooting ratio (80%). No statistical difference was induced by explant types (apical bud or axillary bud) in terms of rooting ratio. In vivo cutting induced rooting rate up to 65% by 0.5% IBA/Talc powder treatment. Although in vivo rooting rate was less efficient compared to in vitro rooting, better survival rate was observed after soil acclimatization. Present study suggested that above micropropagation techniques can be used for rapid multiplication as well as in vitro or in vivo conservation of the species.

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.2
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• pp.110-116
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• 1988

The response of forage yield was studied with various growth indices to develop yield model and to determine optimum cutting time in three cultivars of orchardgrass. 1. Number of tiller per plant was the highest at 3rd cutting stage. But, it was decreased rapidly at 4th cutting stage. Leaf Area Index (LAI) was the highest at 3rd cutting stage. LAI was increased slowly during 15 days to 20 days after cutting and thereafter increased rapidly. 2. In dry matter yield over cutting stages, 1st cutting and 3rd cutting stages were higher yield than others. Change of dry matter yield was similar to that of LAI in all cutting stages. 3. Leaf Elongation Rate (LER) and Specific Leaf Weight (SLW) were reached to maximum at 20 to 25 days and 25 to 30 days after cutting, respectively. 4. Dry matte yield was highly correlated with LAI (r-0.905)and with CGR (r-0.962) over three cultivars. Also, LAI was significantly with LER. The best-fit yield model was obtained in multiple regression equation which included both dependent variables of LAI and CGR. 5. Optimum cutting times which were determined by the relationships between D.M. yield and LAI, and between D.M. yield and CGR, were ranged from 32 days to 36 days depend on each cutting stages.

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.3
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• pp.110-116
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• 1988

The response of forage yield was studied with various growth indices to develop yield model and to determine optimum cutting time in three cultivars of orchardgrass 1. Number of tiller per plant was the highest at 3rd cutting stage. But, it was decreased rapidly at 4th cutting stage. Leaf Area Index (LAI) was the highest at 3rd cutting stage. LA1 was increased slowly during 15 days to 20 days after cutting and thereafter increased rapidly. 2. In dry matter yield over cutting stages, 1st cutting and 3rd cutting stages were higher yield than others. Change of dry matter yield was similar to that of LA1 in all cutting stages. 3. Leaf Elongation Rate (LER) and Specific Leaf Weight (SLW) were reached to maximum at 20 to 25 days and 25 to 30 days after cutting, respectively, 4. Dry matter yield was highly correlated with LA1 (r=0.905)and with CGR (r=0.962) over three cultivars. Also, LA1 was significantly with LER. The best-fit yield model was obtained in multiple regression equation which included both dependent variables of LA1 and CGR. 5. Optimum cutting times which were determined by the relationships between D.M. yield and LAI, and between D.M. yield and CGR, were ranged from 32 days t o 36 days depend on each cutting stages.

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.283-287
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• 2008

To investigate the effects of different wavelengths of photosynthetically active radiation on the morphology and leaf color changes of a single tomato (Lycopersicon esculentum) seedling, we stored the seedling at $10{\pm}0.5^{\circ}C$ under eight different wavelengths (peak wavelengths; 405, 450, 505, 545, 600, 645, 680, and 700 nm) with a constant photosynthetic photon flux of $3{\mu}mol\;m^{-2}s^{-l}$ for 28 d. Under the 405, 450, and 505 nm wavelength conditions, the leaves of the seedlings showed vigorous shape with an upright morphology. Rachis elongation was suppressed and hence compact appearance was observed under the 450 and 505 urn conditions. Although the difference in leaf color between before storage and on 28 days after storage was observed under all wavelength conditions, the 405 and 700 um irradiations changed the leaf color to light green. Application of light-emitting diode (LED) light irradiated from around 450 to 545 nm can contribute to vigorous shape with an upright morphology of tomato seedlings during low light irradiation-low temperature storage.