Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Callus induction and plant regeneration of Lychnis wilfordii (Regel) Maxim a critically endangered plant in Korea

  • Bae, Kee Hwa (National Institute of Biological Resources) ;
  • Lee, Mi Hyun (R&D center, Asiaseed Co. Ltd.) ;
  • Choi, Yong Eui (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Yoon, Eui Soo (Department of Biology, College of Nature Sciences, Kongju National University)
  • Received : 2014.02.26
  • Accepted : 2014.03.17
  • Published : 2014.03.31
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Abstract

Lychnis wilfordii (Regel) Maxim is a rare and valued ornamental plant. Germination rate reached 46.6% when seeds were treated with $100mg{\cdot}l^{-1}$ GA (Gibberellic acid). The highest callus induction was observed in the leaf explants of the seedlings on MS medium containing specific concentrations of $0.5mg{\cdot}l^{-1}$ BA ($N^6$-benzyladenine) and $3.0mg{\cdot}l^{-1}$ NAA (a-naphthalene acetic acid). The adventitious shoot was formed in 97.3% of calli on 1/2 WPM (Woody Plant Medium) medium. Shoot elongation of in vitro propagated plantlets was no difference among various medium. The plantlets grew well after transferring to the pot. This in vitro propagation protocol should be useful for conservation of this endangered plant.

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References

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