The objective of this study was to improve the detection limit of rapid detection kit for Salmonella Typhimurium by image analysis system. The rapid detection kit was comprised of four elements: sample pad, conjugate pad, nitrocellulose pad and absorbent pad. Gold nanoparticle and Salmonella antibody were used as a tag and a receptor. Salmonella antibody and goat rabbit IgG antibody were used as test and control lines on nitrocellulose membrane. The color intensity of test line began to increase from $10^5CFU/mL$ of Salmonella sample. A multiple linear regression analysis was employed to explain the relationship between predicted and measured number of Salmonella cells. The developed model could successfully predict the cell number of Salmonella with validation against extra-experimental result.
Wind tunnel test results and their interpretations, which were performed to study the aerodynamic stability of tower of self-anchored suspension bridge, are presented in this paper. Tower and full models were tested under smooth and turbulent flow conditions. In the case of the tower with inclined two columns, the vibration due to wakes were occurred at wide velocity zone because the wakes with various frequencies were generated by inclined upstream column. It has to be emphasized that the vibration characteristics of the tower in the self-anchored suspension bridge may be very sensitive to the longitudinal boundary conditions of the girder at the supports. Because of the two natural frequency of the tower, out-of-plane bending and torsional, were not well separated, coupled motions were observed in a wide range of wind velocity. The effectiveness of corner cut, countermeasure to reduce the tower vibrations, was also studied. It has been found that 1:10, comer cut size to column width, may be the most effective ratio for reducing the vibrations.
Kim, Hyung Su;Kim, Hee La;Park, Ki Hyung;Cho, Kyung Soon
Clinical and Experimental Pediatrics
/
v.51
no.10
/
pp.1071-1076
/
2008
Purpose : Respiratory syncytial virus (RSV) is the most frequent cause of lower respiratory infections in infants and young children. Early detection allows quarantining of infected inpatients to prevent nosocomial transmission and to choose a treatment. To achieve rapid reporting, to facilitate prompt antiviral therapy, and to avoid unnecessary use of antibiotics, an easy, rapid diagnostic method for RSV is needed. We evaluated a lateral flow immunochromatography (RSV Respi-Strip test) and EIA (Enzyme immuno assay) compared to RT-PCR. Methods : From April 2007 to March 2008, 112 consecutive respiratory specimens (nasopharyngeal aspirates, throat swabs, tracheal aspirates, sputum) from patients who were suffering from the clinical signs and symptoms of respiratory tract infection were enrolled in Busan. A total of 112 patients were tested with RSV Respi-Strip (Corio-BioConcept, Belgium), EIA, and RT-PCR at the same time. Results : Of the 112 specimens tested, the number of children who showed positive results at RT-PCR and Respi-Strip were 45 and 42, respectively. The Respi-Strip rapid antigen test had a sensitivity of 88% and a specificity of 94%. The positive and negative predictive values were 90% and 92%, respectively. The agreement was 83%. Conclusion : In our study, the rapid antigen test had as much sensitivity as any method for detection of RSV. The test has many advantages such as easy performance, simple interpretation, and rapid results. If the rapid antigen test is widely applied in the clinical setting, the may be useful for diagnostic and epidemiological studies of RSV infection.
It is desirable to collect the solar thermal energy at relatively high temperature in order to minimize the size of thermal storage system and to enlarge the scope of solar thermal energy utilization. So far the concentrating solar collector has been developed to collect solar thermal energy at relatively high temperature, but it has some difficulties in maintaining the volumetric body of solar collector for long term utilization. On the other hand, the flat-plate solar collector has been developed to collect the solar thermal energy at low temperature, and it has advantages in maintaining the system for long term utilization, since it's thickness is thin and not volumetric. In this study, to develop a solar collector that has both advantages of collecting solar thermal energy at high temperature and fixing conveniently the collector system for long term period, a cylindrical parabolic concentrating solar collector was designed, which has two rows of parabolic reflectors and thin thickness such as the flat-plate solar collector, maintaining the optical form of concentrating solar collector. The characteristics of the concentrating parabolic solar collector newly designed was analysed and the results are summarized as follows; 1. The temperature of the air enclosed in solar collector was all the same as $50^{\circ}C$ in both cases of the open and closed loop, and when the heat transfer fluid was not circulated in tubular absorber, the maximum surface temperature of the absorber was $118-120^{\circ}C$, this results suggested that the heat transfer fluid could be heated up to $118^{\circ}C$. 2. In case of longitudinal installation of the solar collector, the temperature difference of heat transfer fluid between inlet and outlet was $4^{\circ}-6^{\circ}C$ at the flow rate of $110-130{\ell}/hr$, and the collected solar energy per unit area of collector was $300-465W/m^2$. 3. The collected solar energy per unit area for 7 hours was 1960 Kcal/$m^2$ for the open loop and 220 Kcal/$m^2$ for the closed loop. Therefore it is necessary to combine the open and closed loop of solar collectors to improve the thermal efficiency of solar collector. 4. The thermal efficiency of the solar collector (C.P.C.S.C.) was proportional to the density of solar radiation, indicating the maximum thermal efficiency ${\eta}_{max}=58%$ with longitudinal installation and ${\eta}_{max}=45%$ with lateral installation. 5. The thermal efficiency of the solar collector (C.P.C.S.C.) was increased in accordance with the increase of flow rate of heat transfer fluid, presenting the flow rate of $110{\ell}/hr$ was the value of turning point of the increasing rate of the collector efficiency, therefore the flow rate of $110{\ell}/hr$ was considered as optimum value for the test of the solar collector (C.P.C.S.C.) performance when the heat transfer fluid is a liquid. 6. In both cases of longitudinal and lateral installation of the solar collector (C.P.C.S.C.), the thermal efficiency was decreased linearly with an increase in the value of the term ($T_m-T_a$)/Ic and the increasing rate of the thermal efficiency was not effected by the installation method of solar collector.
Journal of the Korea institute for structural maintenance and inspection
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v.15
no.6
/
pp.118-126
/
2011
The objective of this paper is to study the structural behavior of Composite Basement Wall (CBW) according to shear span-to-depth ratio through an experiment and predict the nonlinear behavior of CBW by using ADINA program widely has been being used for FE analysis. Especially, this study focuses on the part of CBW in which the Reinforced Concrete (RC) is under compression stress; At the region of CBW around each floor, RC part stresses by compressive force when lateral press by soil acts on the wall. The contact condition between RC wall and steel (H-Pile) including stud connector is main factor in the analysis since it governs overall structural behavior. In order to understand the structural behavior of CBW whose RC part is under compressive stress, an experimental work and finite element analysis were performed. Main parameter in the test is shear span-to-depth ratio. For simplicity in analysis, reinforcements were not modeled as a seperated element but idealized as smeared to concrete. All elements were modeled to have bi-linear relation of material properties. Three type of contact conditions such as All Generate Option (AGO), Same Element Group Option with Tie(SEGO-T) and Same Element Group Option with Not tie(SEGO-NT) were considered in the analysis. For each analysis, the stress flow and concentration were reviewed and analysis result was compared to test one. From the test result, CBW represented ductile behavior by contribution of steel member even if it had short shear span-to-depth ration which is close to "1". The global composite behavior of CBW whose concrete wall was under compressive stress could be predicted by using contact element in ADINA program. Especially, the modeling by using AGO and SEGO-T showed more close relation on comparing with test result.
Model tests were performed to investigate the failure modes in embankments on soft ground supported by piles with cap beams. In the model tests, Jumunjin standard sand was placed on simulated cap beams and soft ground. The cap beams are placed perpendicular to the longitudinal axis of the embankment. The colored sand and the Jmniin standard sand were placed one after the other above cap beams and soft ground to make lateral stripes with 3mm thickness in the embarkment. The colored sand was prepared by coating the Jumunjin sand with black lead powder. The photographs illustrate the two characteristic modes of failure in embarkments. One is the soil arching failure and the other is the punching shear failure. The failure mode depends on the height of embankment and the space between cap beams. That is, if the embankment is high enough compared with the space between cap beams, it will fail in arching failure. On the other hand if the embarkment is relatively low or the space between piles is too wide, it will fail in punching shear failure. The soil arching develops in embarkment as a semicylindrical arch with a thickness equal to the width of the cap beam. And the soil wedge developed above the cap beams remains intact during both arching and punching failures. The boundary of punching shear failure of the displaced soil mass can be defined on the basis of observation of the photographs.
We applied functional magnetic resonance imaging (fMRI) techniques to examine whether general intelligence (g) could be assessed using a neurobiological signal of the brain. Participants were students in a national science academy and several local high schools. They were administered diverse intelligence (RAPM and WAIS) and creativity tests (TTCT-figural and TTCT-verbal). Forty of them were scanned using fMRI while performing complex and simple g tasks. In brain regions of greater blood flow in complex compared with simple g tasks, the gifted group with an exceptional g level was not significantly different from the average group with an ordinary g level: both of them activated the lateral prefrontal, anterior cingulate, posterior parietal cortices. However, the activation levels of the gifted group were greater than those of the average group, particularly in the posterior parietal cortex. Correlation analysis showed that the activity of the posterior parietal cortex has the highest correlation ($(r=0.73{\sim}0.74)$) with individual g levels and other regions also have moderate correlation ($(r=0.53{\sim}0.66)$). On the other hand, two-sample t test showed a striking contrast in intelligence tests scores between the gifted and the average group, whereas it did not show in creativity tests scores. These results suggest that it is within the bounds of possibility that a neurobiological signal of the brain is used in the assessment of the gifted and also suggest that creativity has to be given a great deal of weight on the assessment of the gifted.
In this study, the centrifuge tests and numerical analyses were performed to investigate the lateral flow behavior and stability of abutment when high filling was applied on the soft ground improved by SCP. The centrifuge model tests and numerical analyses were fulfilled in the case of the back of abutment filled by EPS (case 1) and soil (case 2), and the potentiometer was installed on the abutment and fill to measure the vertical and horizontal displacement at the top of abutment. As a result of the centrifugal tests, the horizontal displacement of abutment in the case 1 was 1.4cm that is almost coincide with the results of numerical and satisfy the allowable standard. On the other hand, the horizontal displacement of abutment in the case 2 was 12 cm that is 18% greater than that of numerical analysis and exceed the allowable standard. As a result of analysis, the maximum horizontal displacement of pile was 1.26 cm in case 1 that satisfies the criterion of allowable horizontal displacement (1.5 cm). In contrast, the maximum horizontal displacement of pile was 1.005 m in case 2 that greatly exceeds the allowable horizontal displacement.
The aim of this work is to investigate the effect of atmospheric stability on near-field pollutant dispersion from rooftop emissions of a single cubic building using computational fluid dynamics (CFD). This paper used the shear stress transport (here after SST) k-${\omega}$ model for predicting the flow and pollutant dispersion around an isolated cubic building. CFD simulations were performed with two emission rates and six atmospheric stability conditions. The results of the simulations were compared with the data from wind tunnel experiments and the result of simulations obtained by previous studies in neutral atmospheric condition. The results indicate that the reattachment length on the roof ($X_R$) obtained by computations show good agreement with the experimental results. However, the reattachment length of the rooftop of the building ($X_F$) is greatly overestimated compared to the findings of wind tunnel test. The result also shows that the general distribution of dimensionless concentration given by SST k-${\omega}$ at the side and leeward wall surfaces is similar to that of the experiment. In unstable conditions, the length of the rooftop cavity was decreased. In stable conditions, the horizontal velocity in the lower part around the building was increased and the vertical velocity around the building was decreased. Stratification increased the horizontal cavity length and width near surface and unstable stratification decreased the horizontal cavity length and width near surface. Maintained stability increases the lateral spread of the plume on the leeward surface. The concentration levels close to the ground's surface under stable conditions were higher than under unstable and neutral conditions.
The objectives of this study are to develop a state-space form of stochastic dynamic storage function routing model and to test the model performance for real-time flow forecast. The selected study area is the main Han River starting from Paldang Dam site to Indogyo station and the 13 flood events occurred from 1987 to 1998 are selected for computing model parameters and testing the model performance. It was shown that the optimal model parameters are quite different depending on Hood events, but the values used on field work also give reasonable results in this study area. It is also obvious that the model performance from the stochastic-dynamic model developed in this study gives more accurate and reliable results than that from the existing deterministic model. Analysis for allowable forecast lead time leads that under the current time step the reasonable predicted downstream flows in 5 hours time advance are obtained from the stochastic dynamic model on relatively less lateral inflow event in the study area.
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