Browse > Article
http://dx.doi.org/10.3345/kjp.2008.51.10.1071

Clinical usefulness of rapid antigen test to detect respiratory syncytial virus infection  

Kim, Hyung Su (Department of Pediatrics, Busan Medical Center)
Kim, Hee La (Department of Pediatrics, Busan Medical Center)
Park, Ki Hyung (Department of Clinical Pathology, Busan Medical Center)
Cho, Kyung Soon (Bacteriology Division, Busan Metropolitan City Institute of Health & Environment)
Publication Information
Clinical and Experimental Pediatrics / v.51, no.10, 2008 , pp. 1071-1076 More about this Journal
Abstract
Purpose : Respiratory syncytial virus (RSV) is the most frequent cause of lower respiratory infections in infants and young children. Early detection allows quarantining of infected inpatients to prevent nosocomial transmission and to choose a treatment. To achieve rapid reporting, to facilitate prompt antiviral therapy, and to avoid unnecessary use of antibiotics, an easy, rapid diagnostic method for RSV is needed. We evaluated a lateral flow immunochromatography (RSV Respi-Strip test) and EIA (Enzyme immuno assay) compared to RT-PCR. Methods : From April 2007 to March 2008, 112 consecutive respiratory specimens (nasopharyngeal aspirates, throat swabs, tracheal aspirates, sputum) from patients who were suffering from the clinical signs and symptoms of respiratory tract infection were enrolled in Busan. A total of 112 patients were tested with RSV Respi-Strip (Corio-BioConcept, Belgium), EIA, and RT-PCR at the same time. Results : Of the 112 specimens tested, the number of children who showed positive results at RT-PCR and Respi-Strip were 45 and 42, respectively. The Respi-Strip rapid antigen test had a sensitivity of 88% and a specificity of 94%. The positive and negative predictive values were 90% and 92%, respectively. The agreement was 83%. Conclusion : In our study, the rapid antigen test had as much sensitivity as any method for detection of RSV. The test has many advantages such as easy performance, simple interpretation, and rapid results. If the rapid antigen test is widely applied in the clinical setting, the may be useful for diagnostic and epidemiological studies of RSV infection.
Keywords
Respiratory syncytial virus; Rapid antigen test;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Freymath F, Eugene G, Vabret A, Petitjean J, Gennetay E, Brouard J, et al. Detection of respiratory syncytial virus by reverse transcription PCR and hybridization with a DNA enzyme immunoassay. J Clin Microbiol 1995;33:3352-5
2 Waner JL, Whitehurst NJ, Todd SJ, Shalaby H, Wall LV. Comparison of Directigen RSV with viral isolation and direct immunofluorecence for the identification of respiratory syncytial virus. J Clin Microbiol 1990;28:480-3
3 Jennings LC, Anderson TP, Werno AM, Beynon KA, Murdoch DR. Viral etiology of acute respiratory tract infections in children presenting to hospital: role of polymerase chain reaction and demonstration of multiple infections. Pediatr Infect Dis J 2004;23:1003-7   DOI   ScienceOn
4 Gröndahl B, Puppe W, Weigl S, Schimitt HJ. Comparison of the BD Directigen Flu A+B kit and the Abbott TestPack RSV with a multiplex RT-PCR: ELISA for rapid detection of influenza viruses and respiratory syncytial virus. Clin Microbiol Infect 2005;11:848-50   DOI   ScienceOn
5 Barenfanger J, Drake C, Leon N, Mueller T, Trott T. Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study. J Clin Microbiol 2000;38:2824-8
6 Liolios L, Jenney A, Spelman D, Kotsimbos T, Catton M, Wesselingh S. Comparison of a multiplex reverse transcription PCR enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens. J Clin Microbiol 2001;39:2779-83   DOI   ScienceOn
7 Reina J, Gonzales GM, Ruiz DGE. Prospective evaluation of a dot blot enzyme immunoassay (Directgen RSV) for the antigenic detection of respiratory syncytial virus from nasopharyngeal aspirates of paediatric patients. Clin Microbiol Infect 2004;10:967-71   DOI   ScienceOn
8 Swierkosz EM, Flanders R, Melvin L, Miller JD, Kline MW. Evaluation of the Abbott TestPack RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. J Clin Microbiol 1989;27:1151-4
9 Sidwell RW, Barnand DL. Respiratory syncytial virus infections: recent prospects for control. J Antiviral 2006;71:379-90   DOI   ScienceOn
10 Kim SH, Sung JY, Yang MA, Eun BW, Choi EH, Lee HJ, et al. Evaluation of a rapid diagnostic kit "BIOLINE RSVTM" for the detection of respiratory syncytial virus. Korean J Pediatr Infect Dis 2007;14:91-6   DOI
11 Bromberg K, Tannis G, Daidone B, Clarke L, Sierra M. Comparison of Hep-2 cell culture and Abott respiratory syncytial virus enzyme immunoassay. J Clin Microbiol 1987; 25:434-6
12 Thibeault R, Gilca R, Cote S, Serres GD, Boivin G, Dery P. Antibiotics use in children is not influenced by the result of rapid antigen detection test for the respiratory syncytial virus. J Clin Virol 2007;04.013 doi:10.1016
13 Ohm-Smith MJ, Nassos PS, Haller BL. Evaluation of the Binox NOW, BD Directigen and BD Directigen EZ assays for detection of respiratory syncytial virus. J Clin Microbiol 2004;42:2996-9   DOI   ScienceOn
14 Ahluwalia G, Embree J, McNicl P, Law B, Hammond GW. Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzymelinked immunosorbent assay. J Clin Microbiol 1987;25:763-7
15 Aida CC, Barbara BH, Theodor KM, Edward EW, Ann RF. Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults. J Clin Virol 2003;28:169-74   DOI   ScienceOn
16 Henrickson KJ. Advances in the laboratory diagnosis of viral respiratory disease. Pediatr Infect Dis J 2004;23 Suppl 1:S6- 10   DOI
17 Cruz AT, Cazacu AC, Greer JM, Demmler GJ. Performance of a rapid assay (Binax NOW) for detection of respiratory syncytial virus at a Children's Hospital over a three year period. J Clin Microbiol 2007;45:1993-5   DOI   ScienceOn
18 Fitzgeral DA, Kilham HA. Bronchiolitis: assessment and evidence-based management. Med J Aust 2004;180:399-404
19 Kim MR, Lee HR, Lee GM. Epidemiology of acute viral respiratory tract infections in Korean children. J Infect 2000;41:152-8   DOI   ScienceOn
20 Karnauchow TM, Milk R. Rapid detection of RSV: Performance of X/pectTMRSV and RSV Respi-StripTM, Two new immunochromatographic assays. Program and Abstract, the 20th Pan American Society for Clinical Virology (PASCV); 2004 Apr 25-28; Daytona Beach, FL. Florida : The Pan American Society for Clinical Virology, 2004
21 Borek AP, Clemens SH, Gaskins VK, Aird DZ, Valsamabis A. Respiratory syncytial virus detection by Rmal Xpect, Binax NOW RSV, direct immunofluorescent staining and tissue culture. J Clin Microbiol 2006;44:1105-7   DOI   ScienceOn
22 Noyola DE, Zuviri GA, Castro GJA, Rarl OZJ. Impart of respiratory syncytial virus on hospital admissions in children younger than 3 years of age. J Infect 2007;54:180-4   DOI   ScienceOn
23 Parnes C. Palivizumab prophylaxis of respiratory syncytial virus disease in 2000-2001: results from The Palivizumab Outcomes Registry. Pediatr Pulmonol 2003;35:484-9.   DOI   ScienceOn
24 Abels S, Nadal D, Strochle A, Bossart W. Reliable detection of respiratory syncytial virus infection in children for adequate hospital infection control management. J Clin Microbiol 2001;39:3135-9   DOI
25 Rothbarth PH, Hermus MC, Schrijnemakers P. Reliability of two new test kits for rapid diagnosis of respiratory syncytial virus infection. J Clin Microbiol 1991;29:824-6
26 Gregson D, Lloyd T, Buchan S, Church D. Comparison of the RSV Respi-Strip with direct fluorescent antigen detection for diagnosis of respiratory syncytial virus infection in pediatric patients. J Clin Microbiol 2005;43:5782-3   DOI   ScienceOn
27 George KS, Patel NM, Hantwig RA, Scholl DR, Jollick JJA, Kauffmann LM, et al. Rapid and sensitive detection of respiratory virus infections for directed antiviral treatment using R-Mix cultures. J Clin Virol 2002;24:107-15   DOI   ScienceOn
28 Goodrich JS, Miller MB. Comparison of cepheid's analyte specific reagents with BD directigen for detection of respiratory syncytial virus. J Clin Microbiol 2007;45:604-6.   DOI   ScienceOn
29 Zheng X, Quianzon S, Mu Y, Katz BZ. Comparison of two new rapid antigen detection assays for respiratory syncytial virus with another assay and shell vial culture. J Clin Virol 2004;31:130   DOI   ScienceOn
30 Slinger R, Milk R, Gaboury I, Diaz-Mitoma F. Evaluation of the QuickLab RSV test, a new rapid lateral flow immunoassay for detection of respiratory syncytial virus antigen. J Clin Microbiol 2004;42:3731-3   DOI   ScienceOn
31 Kumar ML, Super DM, Lembo RM, Thomas FC, Prokary SL. Diagnostic efficiency of two rapid tests for detection of respiratory syncytial virus antigen. J Clin Microbiol 1987;25: 873-5
32 Fulginiti VA, Eller JJ, Sieber OF, Joyner JW, Minamitari M, Meiklejohn G. Respiratory virus immunization. Part one. A field trial of two inactivated respiratory virus vaccines: An aqueous trivalent parainfluenza virus vaccine and an alumprecipitated respiratory syncytial virus vaccine. Am J Epidemiol 1969;89:435-48   DOI