• Journal of Biosystems Engineering
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• 제40권3호
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• pp.277-283
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• 2015

Background: Foodborne disease outbreaks from various food sources are a major health concern worldwide. Current methods for detection of foodborne pathogens are both expensive and time-consuming. Purpose: This review aims to present the current information available on the use of lateral flow test strips to detect pathogens in food products to enhance food safety. Results: Frequent foodborne disease outbreaks from various food sources have increased the need for rapid and easy methods for routine analysis of foodborne pathogens. Present detection methods for foodborne pathogens require expensive instruments, experts, and long time for sample analysis. Lateral flow test strips have drawn attention in recent years because of their ability to detect analytes quickly and easily. This review focuses on the principle of the lateral flow test, the various formats of lateral flow test strips, recognition elements, labeling tags, and reading instruments. In addition, this review also discusses the future prospects for the lateral flow test strips.

• Journal of Biosystems Engineering
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• 제38권4호
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• pp.335-340
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• 2013

Purpose: This study was performed to develop a lateral flow strip sensor for the detection of pathogenic Escherichia coli O157:H7 in various samples. Also, feasibility of using an image analysis method to improve the interpretation of the strip sensor was evaluated. Methods: The lateral flow strip sensor has been fabricated based on nitrocellulose lateral-flow membrane. Colloidal gold and E. coli O157:H7 antibodies were used as a tag and a receptor, respectively. Manually spotted E. coli O157:H7 antibody and anti-mouse antibody on nitrocellulose membrane were used as test and control dots, respectively. Feasibility of the lateral flow strip sensor to detect E. coli O157:H7 were evaluated with serially diluted E. coli O157:H7 cells in PBS or food samples. Test results of the lateral flow strip sensor were measured with an image analysis method. Results: The intensity of the test dot started to increase with higher concentration of the cells were introduced. The sensitivities of the sensor were both $10^4$ CFU/mL Escherichia coli O157:H7 spiked in PBS and in chicken meat extract, respectively. Conclusions: The lateral flow strip sensor and image analysis method could detect E. coli O157:H7 in 20 min, which is significantly quicker than conventional plate counting method.

• 농업과학연구
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• 제46권3호
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• pp.549-557
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• 2019

Staphylococcal enterotoxin is a very common cause of food poisoning. Conventional detection methods for the toxin including enzyme-linked immunosorbent assays (ELISAs), chemiluminescence (ECL), and polymerase chain reaction (PCR) assays require a lot of time, efforts, and expert technicians. Lateral flow strip kits have shown great potential for the rapid detection of foodborne pathogens. The lateral flow strip kit is widely used in clinical settings because it is easy to use, fast, and cost effective. A typical lateral flow strip kit uses colloidal gold to generate a visual signal. However, the lateral flow strip kit based on colloidal gold has limited sensitivity to fulfill food safety regulation requirements. This study was performed to develop a rapid test kit for pathogenic staphylococcal enterotoxin B (SEB) in food samples. The rapid detection kit was fabricated based on a nitrocellulose lateral-flow strip. Cellulose nano beads and SEB antibodies were used as the tag and receptor, respectively, to improve the detection performance. Manually spotted SEB antibody and anti-rabbit antibody on the surface of the nitrocellulose membrane were used as test and control spots, respectively. The feasibility of the rapid test kit to detect SEB in samples was evaluated. The sensitivity of the kit was 10 ng/mL SEB spiked in PBS. Additionally, the rapid test kit could detect 1 ng/mL of SEB in chicken meat extract.

• 한국지반환경공학회 논문집
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• 제12권11호
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• pp.65-70
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• 2011

모래지반의 액상화 발생 가능성 및 측방유동 평가 방법에 대한 연구는 많이 알려져 있다. 최근에는 비소성 실트의 액상화 발생 가능성에 대해서도 연구하고 있다. 그러나 비소성실트의 동적하중이 작용하는 동안과 그 이후의 거동에 대해서는 세계적으로도 다소 알려져 있을 뿐이다. 특히 비소성실트의 측방유동에 대한 평가방법에 대해서는 거의 알려져 있지 않다. 이에 본 연구에서는 비소성실트지반에서의 액상화 발생 가능성 및 측방유동량의 평가 방법을 제안하는 것을 목적으로 하였다. 연구방법은 정적 및 동적 시험을 수행할 수 있는 직접단순전단시험기를 이용하여 동적하중을 받은 비소성실트의 액상화 가능성을 평가하였으며, 그 후 정적 시험을 수행하여 잔류강도를 측정하였다. 이 시험결과 비소성실트는 유효응력이 완전이 소멸되는 것은 곤란하지만 액상화에 거의 가깝게 발생하는 것을 확인하였다. 특히, 전단응력의 증가에 따른 강도저하는 매우 현저한 것을 확인하였다. 이 시험결과를 이용하여 액상화 발생 가능성 및 측방유동량을 추정할 수 있는 방법을 제안하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권2호
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• pp.127-131
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• 2004

A low-cost, simple strip reader system using a linear movement mechanism of CD-ROM deck has been developed to characterize a lateral flow membrane-based immunochromatographic assay. The test strip reader was assembled by a CD-ROM deck and home-made optical head especially designed for immunoassays. The optical head for detecting reflected light from the test strip surface consists of green light-emitting diode, large area silicon photodiode, and anodized aluminum mounting block providing a slit structure for cutting light from the LED. The stepping motor of the deck was operated in the full step mode, whose distance of each reading point is about 0.15mm. The performance of the strip reader was tested by analysis of HBV(hepatitis B virus) antigen test kit. This strip reader can be useful for inexpensive, disposable, and membrane-based assays that provide visual evidence of the presence of an analyte in a liquid sample.

• Geomechanics and Engineering
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• 제35권1호
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• pp.13-27
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• 2023

The model production for large-scale (lateral length ≥ 2.0 m) capillary barrier (CB) model tests is time and cost-intensive. To address these limitations, the framework of a small-scale CB (SSCB) model test under the lateral no-flow condition has been established. In this study, to validate the experimental methodology of the SSCB model test, a series of seepage analyses on the SSCB model test and engineered slopes in the same and additional test conditions was performed. First, the seepage behavior and diversion length (L_D) of the CB system were investigated under three rainfall conditions. In the seepage analysis for the engineered slopes with different slope angles and sand layer thicknesses, the L_D increased with the increase in the slope angle and sand layer thickness, although the increase rate of the L_D with the sand layer thickness exhibited an upper limit. The L_D values from the seepage analysis agreed well with the results estimated from the laboratory SSCB mode test. Therefore, it can be concluded that the experimental methodology of the SSCB model test is one of the promising alternatives to efficiently evaluate the water-shielding performance of the CB system for an engineered slope.

• 한국철도학회논문집
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• 제7권2호
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• pp.149-154
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• 2004

Pile Bridge Abutments constructed on a soft base are affected by a lateral flow. Laterl flow pressure acting on Pile is very difficult to calculate because of, interation of ground and Pile. So, it is different to estimate displacement of Pile Bridge Abutments. This paper studied about possibility of the displacement estimation of Pile Bridge Abutments by using the equivalent sheet pile wall theory that was Randolph proposed in 1981. Analysis program through using the SAGE CRISP that is FEM program. Analysis data used Centrifuge test results of Springman(1991), Bransby(1997) and Ellis(1997)'s paper. In conclusion, maxium displacement that is carried out by centrifuge test and numerical analysis has occured at the head of pile, as well as Maximum displacement of pile is closely similar. But the moment acting on pile of numerical analysis is under estimated compare to the centrifuge test. Through the comparative study, it is found that displacement estimation by equivalent sheet pile wall is in relatively good agreement with the results of centrifuge test.

• 미생물학회지
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• 제44권4호
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• pp.346-351
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• 2008

본 연구에서는 임신진단등의 간편 현장진단(point of care test, POCT)에 주로 사용되고 있는 멤브레인 측면흐름 분석기법을 사용하여 인유두종 바이러스(Human papillomavirus, HPV)의 특정 서열을 검출할 수 있는 DNA array를 개발하였다. HPV type 6, 11, 16, 18, 31, 45에 특이적인 DNA 탐침들을 측면흐름 분석용 멤브레인 표면에 고정하고, biotin이 label된 MY09/11 primer를 사용하여 얻어진 HPV PCR 반응 결과물과 탐침 사이에 hybridization 반응을 유도하였다. 이후 streptavidin이 label된 colloidal gold가 교잡물의 biotin과 반응함으로써 DNA hybridization 결과를 육안으로 확인할 수 있었다. 본 연구를 통해 개발된 HPV DNA lateral flow membrane array는 기존의 HPV DNA chip 기법과 비교하여 경제적이고 편리하게 주요 HPV type을 확인할 수 있음을 보여주었다.

• 지질공학
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• 제12권1호
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• pp.75-88
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• 2002

오염된 연약지반에 편재하중이 작용하게 되면 지반 중에 침하, 측방변위, 융기 및 전단파괴 등의 큰 소성 전단변형이 발생하게 되고, 때로는 지반과 구조물에 막대한 손상을 초래하게 된다. 따라서 본 연구는 오염된 연약지반의 식내모형실험을 실시하였으며 측방유동압의 결정방법을 기존의 이론식과 비교하여 분석하였다. 모형실험은 먼저 모형재하장치를 제작하고 토조 안에 오염된 지반시료를 채워 비배수상태에서 하중을 인정한 시간 간격으로 재하하여 변형상태를 관측하였다. 그 결과 편재 하중을 받는 오염된 연약지반에서의 측방유동압은 P=K$_{0}$YH식에 의해서 산정함이 비교적 적절하며, 측방유동압의 최대값은 토층두께(H)의 0.3H 부근에서 발생하였다. 또한 종합형과 Poulos의 분포형태 및 오염되지 않는 연약점토(CL, CH) 지반보다 지표면측으로 상승하여 발생하였다.

• 한국지반신소재학회논문집
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• 제10권1호
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• pp.43-51
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• 2011

연약 점성토 지반에 성토를 할 경우 과도한 침하와 측방유동으로 인한 피해를 최소화하기 위해 토목섬유/성토지지말뚝공법이 주로 사용되고 있다. 본 공법은 연약지반 층을 관통하여 지지층에 관입시킴으로써 상재하중을 지지하게 하여 상재하중으로 인한 침하와 측방유동 발생을 최소화한다. 본 연구에서는 연약 점성토 지반에 설치된 토목섬유/성토지지말뚝공법의 보강효과를 정량적으로 검토함을 목적으로 하였다. 실내모형실험을 통하여 무보강 및 성토지지말뚝 보강의 경우에 대한 지반 거동을 재현하고 성토지지말뚝에 의한 측방유동 억지효과를 분석하였다. 이때 성토지지말뚝을 설치한 경우에 대해서는 상재하중의 크기를 다르게 설정하여 완속 재하와 급속 재하의 경우에 대한 측방유동의 억지효과를 분석하였다.