Browse > Article
http://dx.doi.org/10.5307/JBE.2013.38.4.335

Image Analysis of a Lateral Flow Strip Sensor for the Detection of Escherichia coli O157:H7  

Kim, Giyoung (National Academy of Agricultural Science)
Moon, Ji-Hea (National Academy of Agricultural Science)
Park, Saet Byeol (National Academy of Agricultural Science)
Jang, Youn-Jung (National Academy of Agricultural Science)
Lim, Jongguk (National Academy of Agricultural Science)
Mo, Changyeun (National Academy of Agricultural Science)
Publication Information
Journal of Biosystems Engineering / v.38, no.4, 2013 , pp. 335-340 More about this Journal
Abstract
Purpose: This study was performed to develop a lateral flow strip sensor for the detection of pathogenic Escherichia coli O157:H7 in various samples. Also, feasibility of using an image analysis method to improve the interpretation of the strip sensor was evaluated. Methods: The lateral flow strip sensor has been fabricated based on nitrocellulose lateral-flow membrane. Colloidal gold and E. coli O157:H7 antibodies were used as a tag and a receptor, respectively. Manually spotted E. coli O157:H7 antibody and anti-mouse antibody on nitrocellulose membrane were used as test and control dots, respectively. Feasibility of the lateral flow strip sensor to detect E. coli O157:H7 were evaluated with serially diluted E. coli O157:H7 cells in PBS or food samples. Test results of the lateral flow strip sensor were measured with an image analysis method. Results: The intensity of the test dot started to increase with higher concentration of the cells were introduced. The sensitivities of the sensor were both $10^4$ CFU/mL Escherichia coli O157:H7 spiked in PBS and in chicken meat extract, respectively. Conclusions: The lateral flow strip sensor and image analysis method could detect E. coli O157:H7 in 20 min, which is significantly quicker than conventional plate counting method.
Keywords
E. coli O157:H7; Food safety; Image analysis; Lateral flow; Strip sensor;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Cho, H. K., G. Y. Kim, W. H. Kim and M. S. Sung. 2010. Detection of Pathogenic Salmonella Using a Surface Plasmon Resonance Biosensor. J. of Biosystems Eng. 35(2):116-123.   과학기술학회마을   DOI   ScienceOn
2 Chua, A., C. Y. Yean, M. Ravichandran, B. Lim and P. Lalitha. 2011. A rapid DNA biosensor for the molecular diagnosis of infectious disease. Biosensors and Bioelectronics 26(9):3825-3831.   DOI   ScienceOn
3 Hart, R. W., M. G. Mauk, C. Liu, X. Qiu, J. A. Thompson, D. Chen, D. Malamud, W. R. Abrams and H. H. Bau. 2011. Point-of-care oral-based diagnostics. Oral Diseases 17:745-752.   DOI   ScienceOn
4 Hossain, S. M. Z., C. Ozimok, C. Sicard, S. D. Aguirre, M. M. Ali, Y. Li and J. D. Brennan. 2012. Multiplexed paper test strip for quantitative bacterial detection. Anal. Bioanal. Chem. 403:1567-1576.   DOI   ScienceOn
5 Karch, H., P. Tarr and M. Bielaszewska. 2005. Enterohaemorrhagic Escherichia coli in human medicine. Int. J. Med. Microbiol. 295(6-7):405-18.   DOI   ScienceOn
6 Kim, G., G. M. Yang, Y. H. Kim, C. Y. Mo and S. B. Park. 2010. Detection of Pathogenic Salmonella with a Composite Quantum Dot. J. of Biosystems Eng. 35(6):458-463.   DOI   ScienceOn
7 Kim, G., G. Yang, S. B. Park, Y. H. Kim, K. J. Lee, J. Y. Son, H. J. Kim and S. R. Lee. 2011. Rapid Detection Kit for Salmonella typhimurium. J. of Biosystems Eng. 36(2): 140-146.   과학기술학회마을   DOI   ScienceOn
8 Kim, G., J. H. Moon and M. Morgan. 2013. Multivariate data analysis of impedimetric biosensor responses from Salmonella typhimurium. Analytical Methods. 5: 4074-4080.   DOI
9 Kuang, H., G. Cui, X. Chen, H. Yin, Q. Yong, L. Xu, C. Peng, L. Wang and C. Xu. 2013. A one-step homogeneous sandwich immunosensor for Salmonella detection based on magnetic nanoparticles (MNPs) and quantum dots (QDs). Int. J. Mol. Sci. 14:8603-8610.   DOI   ScienceOn
10 Posthuma-Trumpie, G. A., J. Korf and A. Amerongen. 2009. Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey. Anal Bioanal. Chem. 393:569-582.   DOI   ScienceOn
11 Radke, S. A. and E. C. Alocilja. 2005. A high density microelectrode array biosensor for detection of E. coli O157:H7. Biosens. Bioelectron. 20:1662-1667.   DOI   ScienceOn
12 Su, X. and Y. Li 2004. Quantum dot biolabeling coupled with immunomagnetic separation for detection of Escherichia coli. O157:H7. Anal. Chem. 76:4806-4810.   DOI   ScienceOn
13 Zhu, H., U. Sikora and A. Ozcan. 2012. Quantum dot enabled detection of Escherichia coli using a cell-phone. Analyst 137:2541-2544.   DOI   ScienceOn
14 Varshney, M. and Y. B. Li. 2007. Interdigitated array microelectrode based impedance biosensor coupled with magnetic nanoparticle-antibody conjugates for detection of Escherichia coli O157:H7 in food samples. Biosens. Bioelectron. 22:2408-2414.   DOI   ScienceOn
15 Wang, D. B., B. Tian, Z. P. Zhang, J. Y. Deng, Z. Q. Cui, R. F. Yang, X. Y. Wang, H. P. Wei and X. E. Zhang. 2013. Rapid detection of Bacillus anthracis spores using a super-paramagnetic lateral-flow immunological detection system. Biosens Bioelectron. 42:661-667.   DOI   ScienceOn
16 Wang, H., Y. Li and M. Slavik. 2007. Rapid detection of Listeria monocytogenes using quantum dots and nanobeads-based optical biosensor. Journal of Rapid Methods & Automation in Microbiology 15:67-76.   DOI   ScienceOn