• Title/Summary/Keyword: lateral flow

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An Inexpensive System for Rapid and Accurate On-site Detection of Garlic-Infected Viruses by Agarose Gel Electrophoresis Followed by Array Assay

  • Kazuyoshi Furuta;Shusuke Kawakubo;Jun Sasaki;Chikara Masuta
    • The Plant Pathology Journal
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    • v.40 no.1
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    • pp.40-47
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    • 2024
  • Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.

Counter-Current Air-Water Flow in Narrow Rectangular Channels With Offset Strip Fins

  • Kim, Byong-Joo;Sohn, Byung-Hu;Koo, Kee-Kahb
    • Journal of Mechanical Science and Technology
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    • v.17 no.3
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    • pp.429-439
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    • 2003
  • Counter-current two-phase flows of air- water in narrow rectangular channels with offset strip fins have been experimentally investigated in a 760 mm long and 100 mm wide test section with 3.0 and 5.0 mm gap widths. The two-phase flow regime, channel-average void fractions and two-phase pressure gradients were studied. Flow regime transition occurred at lower superficial velocities of air than in the channels without fins. In the bubbly and slug flow regimes, elongated bubbles rose along the subchannel formed by fins without lateral movement. The critical void fraction for the bubbly-to-slug transition was about 0.14 for the 3 mm gap channel and 0.2 for the 5 mm gap channel. respectively. Channel-average void fractions in the channels with fins were almost the same as those in the channels without fins. Void fractions increased as the gap width increased, especially at high superficial velocity of air. The presence of fins enhanced the two-phase distribution parameter significantly in the slug flow, where the effect of gap width was almost negligible. Superficial velocity of air dominated the two-phase pressure gradients. Liquid superficial velocity and channel gap width has only a minor effect on the pressure gradients.

A mathematical model of blood flow and convective diffusion processes in constricted bifurcated arteries

  • Chakravarty S.;Sen S.
    • Korea-Australia Rheology Journal
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    • v.18 no.2
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    • pp.51-65
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    • 2006
  • Of concern in the present theoretical investigation is the study of blood flow and convection-dominated diffusion processes in a model bifurcated artery under stenotic conditions. The geometry of the bifurcated arterial segment having constrictions in both the parent and its daughter arterial lumen frequently appearing in the diseased arteries causing malfunction of the cardiovascular system, is constructed mathematically with the introduction of suitable curvatures at the lateral junction and the flow divider. The streaming blood contained in the bifurcated artery is treated to be Newtonian. The flow dynamical analysis applies the two-dimensional unsteady incompressible nonlinear Wavier-Stokes equations for Newtonian fluid while the mass transport phenomenon is governed by the convection diffusion equation. The motion of the arterial wall and its effect on local fluid mechanics is, however, not ruled out from the present model. The main objective of this study is to demonstrate the effects of constricted flow characteristics and the wall motion on the wall shear stress, the concentration profile and on the mass transfer. The ultimate numerical solutions of the coupled flow and diffusion processes following a radial coordinate transformation are based on an appropriate finite difference technique which attain appreciable stability in both the flow phenomena and the convection-dominated diffusion processes.

Tilt variation and wake turbulence in the otter board of a bottom trawl during fishing operations

  • KIM, Yong-Hae
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.53 no.4
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    • pp.337-348
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    • 2017
  • The tension of warp from trawler and sea-floor contact can generate tilt and wake turbulence around otter boards. Preliminary measurements of otter board tilt and 3-D flow velocity during bottom trawl operations were taken using a vector instrument to investigate the effects of wake turbulence at the trailing edge of the otter board. Tilt data (i.e., yaw, pitch, and roll) at 1 Hz and flow data (velocities in the towing, lateral, and vertical directions) at 16 Hz were analyzed to determine their periods and amplitudes using global wavelet and peak event analyses. The mean period (${\pm}standard$ deviation) of the tilt from the peak event analysis ($5{\pm}2s$) was longer or double than that of flow velocity ($3{\pm}2s$). The two periods also had a significant linear relationship. The turbulence rate of flow was 30-50% at the trailing edge and was closely related to roll deviation. The frequency of phase difference ratios (i.e., peak time differences between tilts and flow periods) was significantly different from random occurrence in two trials, possibly due to side tidal effects. However, in the other trials, flow peaks were random, as shown by the even peak times between tilts and flows. Future studies should focus on reducing tilt variation, wake turbulence, and bottom contact to stabilize otter board motion.

Numerical analysis of lateral geomorphology changes by channel bed deposition and bank erosion at the river confluence section (합류부 구간에서의 하상퇴적과 하안침식에 의한 평면적 하도변화 수치모의)

  • Ji, Un;Jang, Eun Kyung
    • Journal of Korea Water Resources Association
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    • v.49 no.5
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    • pp.391-398
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    • 2016
  • The confluence section of rivers forms complex flow pattern due to inflow discharge variation at the mainstream and tributary. Due to complex flow characteristics, bed change and bank erosion at the local section produce lateral geomorphology changes in rivers. In this study, bankline change by bank erosion and bed change were simulated using CCHE2D of 2-dimensional numerical model for quantitative analysis of lateral changes in the confluence section of South Han River and Geumdang Stream. As a result, bankline at the left-side channel of the mainstream was largely changed in the downstream section of the confluence compared to the upstream section. Also, bank erosion in the tributary was hardly occurred and bankline at the left-side tributary and right-side main stream moved to riverside land due to decreased velocity and deposition.

Field measurement study on snow accumulation process around a cube during snowdrift

  • Wenyong Ma;Sai Li;Xuanyi Zhou;Yuanchun Sun;Zihan Cui;Ziqi Tang
    • Wind and Structures
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    • v.37 no.1
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    • pp.25-38
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    • 2023
  • Due to the complexity and difficulty in meeting the multiphase flow complexity, similarity, and multiscale characteristics, the mechanism of snow drift is so complicated that the snow deposition prediction is still inaccurate and needs to be far improved. Meanwhile, the validation of prediction methods is also limited due to a lack of field-measured data about snow deposition. To this end, a field measurement activity about snow deposition around a cube with time was carried out, and the snow accumulation process was measured under blowing snow conditions in northwest China. The maximum snow depth, snow profile, and variation in snow depth around the cube were discussed and analyzed. The measured results indicated three stages of snow accumulation around the cube. First, snow is deposited in windward, lateral and leeward regions, and then the snow depth in windward and lateral regions increases. Secondly, when the snow in the windward region reaches its maximum, the downwash flow erodes the snow against the front wall. Meanwhile, snow range and depth in lateral regions have a significant increase. Thirdly, a narrow road in the leeward region is formed with the increase in snow range and depth, which results in higher wind speed and reforming snow deposition there. The field measurement study in this paper not only furthers understanding of the snow accumulation process instead of final deposition under complex conditions but also provides an important benchmark for validating prediction methods.

Deterministic Lateral Displacement as a Function of Particle Size Using a Piecewise Curved Planar Interdigitated Electrode Array (다중예각 평면 교차전극을 이용한 입자 크기에 따른 측면방향 변위)

  • Han, Song-I;Joo, Young-Dong;Han, Ki-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.36 no.3
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    • pp.241-249
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    • 2012
  • This paper presents the lateral displacement of a particle passing over a planar interdigitated electrode array at an angle as a function of particle size. The lateral displacement was also measured as a function of the angle between the electrode and the direction of flow. A simplified line charge model was used for numerical estimation of the lateral displacement of fluorescent polystyrene (PS) beads with three different diameters. Using the lateral displacement as a function of particle size, we developed a lateral dielectrophoretic (DEP) microseparator, which enables the continuous discrimination of particles by size. The experiment using an admixture of 3-, 5-, and $10-{\mu}m$ PS beads showed that the lateral DEP microseparator could continuously separate out 99.86% of the $3-{\mu}m$ beads, 98.82% of the $5-{\mu}m$ beads, and 99.69% of the $10-{\mu}m$ beads. The lateral DEP microseparator is thus a practical device for the simultaneous separation of particles according to size from a heterogeneous admixture.

Evaluation of Lateral Flow in Soft Ground under Embankment (성토하부 연약지반의 측방유동 평가)

  • Hong, Won-Pyo;Cho, Sam-Deok;Lee, Jae-Ho;Lee, Kwang-Wu
    • Journal of the Korean Geotechnical Society
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    • v.22 no.10
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    • pp.93-100
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    • 2006
  • The lateral soil movement in soft grounds undergoing improvement with application of vertical drains is analyzed on the basis of monitoring data at three fields, in which fifty six monitoring sites are located. Based on the investigations, the criterions are suggested to predict the lateral soil movement. In order to predict the lateral soil movement in the improved soft grounds by using the dimensionless parameter R suggested by Marche & Chapuis (1974), it is desirable that the maximum lateral displacement in the soft ground below the toe of embankment should be applied to calculate R instead of the lateral displacement at the toe of embankment. The lateral soil movement may increase rapidly, if the safety factor of slope is less than 1.4 in case of high ratio of H/B (Thickness of soft ground/Embankment width) such as 1.15 or is less than 1.2 in case of low ratio of H/B such as 0.05. Also, the graph suggested by Tschebotarioff (1973), which illustrates the relationship between the maximum height of embankments and the undrained shear strength of soft grounds, can be applied to the evaluation for the possibility of the lateral soil movement due to embankments on soft grounds.

Aeroelastic Behaviors of Self-anchored Suspension Bridge with Lateral Sag of Main Cable(I) -Focused on the Behavior of Girder- (횡방향 새그를 가진 자정식 현수교의 공탄성 거동(I) -주형의 거동을 중심으로-)

  • Kwon, Soon Duck;Chang, Sung Pil
    • Journal of Korean Society of Steel Construction
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    • v.9 no.2 s.31
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    • pp.259-267
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    • 1997
  • Wind tunnel test results and their interpretations focused on the behavior of girder, which were performed to study the aerodynamic stability of a self-anchored suspension bridge with lateral sag of main cable, are presented in this paper The shape of the girder which has the best aerodynamic stability was selected based on the section model test under uniform and turbulent flow conditions. Good performance of the selected section was confirmed in the full bridge model test. Measured flutter derivatives are presented for further study. Buffeting response was investigated to check the fatigue problem and serviceability of the bridge but it was found to be acceptable from the engineering point of view. Even though the drag coefficient of the girder had high value, the amplitude of the lateral vibration was found to be very low. This may be due to the restraint provided by the lateral sag of the cables.

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Roughness Coefficient of Collector Well Lateral in a Model Riverbed Filtration (하상여과 모형에서 수평집수관의 조도계수)

  • Kim, Woo-Chul;Kim, Seung-Hyun
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.2
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    • pp.176-183
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    • 2007
  • In order to analyze the experimental results obtained from the model riverbed filtration performed by Ahn et al. a mathematical model was developed to simulate the flow through the lateral. The discharge rates at each section of the lateral measured by Ahn et al. were compared with the model predictions, and they matched favorably. The Manning's roughness coefficients of all the laterals employed in the study of Ahn et al. were determined using the model. Results show that the roughness coefficient becomes larger with the increase in the entrance velocity to the collector well, and that the coefficient ranges from 0.012 to 0.015 under the normal operational conditions of the riverbed filtration. Results also show that the coefficient becomes smaller as the lateral diameter increases.