• Journal of Biosystems Engineering
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• v.36 no.2
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• pp.140-146
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• 2011

This study was performed to develop a rapid test kit for pathogenic Salmonella in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and biotin conjugated Salmonella antibodies were used as a tag and a receptor, respectively. Manually spotted Salmonella antibody and Neutravidin on nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Salmonella typhimurium in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Salmonella spiked in PBS. Also, the rapid test kit could detect $10^6$ cfu/mL of Salmonella in chicken meat extract.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.13 no.1
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• pp.26-32
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• 2003

The vibrational system of this study is consisted of a rotating cantilever pipe and the flow in the pipe. The equation of motion is derived by using Lagrange equation. The influences of the rotating angular velocity and the velocities of fluid flow in the pipe have been studied on the dynamic characteristics of a rotating cantilever pipe by numerical method. The tip-amplitude of axial vibration and maximum tip-deflection of axial direction of cantilever pipe are directly proportional to the velocity of fluid and rotating angular velocity of pipe In the steady state. respectively The bending tip-amplitude of cantilever pipe is inversely proportional to the velocity of fluid in the steady state. When the rotating angular velocity is 5 rad/s, the velocity of fluid increase with increasing the natural frequency of axial vibration at second mode and third mode, but the natural frequency axial direction of first mode is decreased. The natural frequency of lateral direction is decreased due to increase of the rotating angular velocity. It identifies that the Influence of velocity of fluid give much variation lower mode of vibration in lateral direction. And the Influence of velocity of fluid give much variation higher mode of vibration in axial direction.

• Magazine of the Korean Society of Agricultural Engineers
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• v.11 no.3
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• pp.1707-1718
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• 1969

The purpose of this experiment is to determine the relations between numbers, arrangements and pumping rates of wells. In this experiment, well pipes were vertically set up in an artificial water-bearing sand layer of homogeneous quality. Wells were arranged in different ways and their number was varied in order to observe the variation of pumping rates. Sands were filled in a square tank, $183cm{\times}91.5cm{\times}91.5cm$ so as to secure a water-bearing layer. Water was constantly supplied from a supply tank located at an end of the tank. The number of well pipes was varied from one to four. Well pipes were connected by a horizontal header pipes were connected by a horizontal header pipe located above them and one pump was used. Pumping rates were measured, when they were arranged in longitudinal and lateral directions, They were also arranged in a square and triangle. The main results thus obtained are presented as follows: (1) When well pipes are laid out in a longitudinal line, i. e., in a flow direction, the ratios of pumping rates of one-pipe well and wells 2-, 3- and 4- pipe t, are 1.903, 2.506 and 2.66, respectively. (2) When well pipes are laid out in a lateral line, i.e., in a perpendicular direction to flow, the same ratios as (1) are 1.912, 2.527 and 2.88. (3) When four pipes are laid out in a square and three pipes are laid out in a triangle, pumping rates are deereased, Comparing with the cases when pipes are laid out in a straight line.

• Transactions of Materials Processing
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• v.18 no.6
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• pp.453-457
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• 2009

In this paper, plastic deformation behaviors of ESW105 and SCM435 steels are revealed by simulations and experiments. ESW105 is the special pre-heat-treated steel characterized by high initial yield strength and negligible strain-hardening behavior. The flow stresses of the two steels for large stain are calculated from tensile tests. Axial and lateral compressions of cylindrical bars are tested and simulated and the deformed shapes are compared to characterize the plastic deformation behaviors of the two materials. A forward extrusion process of a cylindrical bar is also simulated to reveal the difference. It has been shown that there are pretty much difference in plastic flow between ESW105 and SCM435 which causes from the difference in strain-hardening capability, implying that the experience-oriented design rules for common commercial materials may lead to failure in process design when the new material of ESW105 is applied without consideration of its plastic deformation behavior.

• Korean Journal of Agricultural Science
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• v.40 no.2
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• pp.139-146
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• 2013

This study was performed to develop a rapid test kit for pathogenic Staphylococcus in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and Staphylococcus antibodies were used as a tag and a receptor, respectively. Manually spotted Staphylococcus antibody and anti-mouse antibody on the surface of nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Staphylococcus aureus in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Staphylococcus spiked in PBS. Also, the rapid test kit could detect $10^5$ cfu/mL of Staphylococcus in chicken meat extract.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.05a
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• pp.48-51
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• 2009

In this paper, plastic deformation behaviors of ESW105 and SCM435 steels are revealed by simulations and experiments. ESW105 is the special pre-heat-treated steel characterized by high initial yield strength and negligible strain-hardening behavior. The flow stresses of the two steels for large stain are calculated from tensile tests. Axial and lateral compressions of cylindrical bars are tested and simulated and the deformed shapes are compared to characterize the plastic deformation behaviors of the two materials. A forward extrusion process of a cylindrical bar is also simulated to reveal the difference. It has been shown that there are pretty much difference in plastic flow between ESW105 and SCM435 which causes from the difference in strain-hardening capability, implying that the experience-oriented design rules for common commercial materials may lead to failure in process design when the new material of ESW105 is applied without consideration of its plastic deformation behavior.

• Tribology and Lubricants
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• v.28 no.5
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• pp.218-232
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• 2012

In a spool valve analysis, the Reynolds equation is commonly used to investigate the lubrication characteristics. However, the validity of the Reynolds equation is questionable in a spool valve analysis because cavitation often occurs in the groove and the depth of the groove is much higher than the clearance in most cases. Therefore, the validity of the Reynolds equation in a spool valve analysis is investigated by comparing the results obtained from the Reynolds equation and the Navier-Stokes equation. Dimensionless parameters are determined from a nondimensional form of the governing equations. The differences between the lateral force, friction force, and volume flow rate (leakage) obtained by the Reynolds equation and those obtained by the Navier-Stokes equation are discussed. It is shown that there is little difference (less than 10%), except in the case of a spool valve with many grooves where no cavitation occurs in the grooves. In most cases, the Reynolds equation is effective for a spool valve analysis under a no cavitation condition.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.388-388
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• 2019

하도 홍수추적의 방법은 크게 수리학적 방법과 수문학적 방법으로 구분할 수 있다. 수리학적 홍수추적 방법은 정확하지만 대량의 자료가 필요하고 시간이 오래 걸린다. 이와 반대로 수문학적 홍수추적 방법은 정확성은 떨어지지만 소량의 자료만 있으면 되고 시간이 적게 걸린다. 여러 수문학적 홍수추적에 관한 연구들이 있으며 대표적으로 Muskingum 방법이 있다. Muskingum 방법 중 Linear Muskingum Model(LMM)은 방정식의 구조적 한계 때문에 정확한 홍수추적이 어려웠고, 이를 개선하기위하여 Nonlinear Muskingum Model(NLMM), Nonlinear Muskingum Model Incorporation Lateral Flow(NLMM-L) 및 Advanced Nonlinear Muskingum Model Incorporating Lateral Flow(ANLMM-L)이 제안되었다. 본 연구는 수문학적 홍수추적 중 Muskingum 방법의 결과 차이가 어떤 요인으로 인해 발생하는지 검토하였다. 최적화 알고리즘으로 화음탐색법(Harmony Search, HS)을 사용하였으며 LMM, NLMM, NLMM-L 및 ANLMM-L의 매개변수를 산정하였다. 각 방법에 적용 시 HS의 매개변수에 변화를 주어 민감도 분석을 실시하였으며, 분석을 위한 홍수자료는 The Willson Flood data (1947)를 선택하였다. 오차비교방법은 Sum of Squares(SSQ), Root Mean Square Errors(RMSE), Nash-Sutcliffe Efficiency(NSE)를 비교하였다. 비교 결과 알고리즘의 성능에 의한 차이보다 홍수추적 방법의 차이가 더 영향이 큰 것으로 나타났다.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2011.04a
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• pp.211-214
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• 2011

In the present work, a computational study was conducted to investigate characteristic of lateral force on the flow fields inside the propulsion nozzle with step. The unsteady, compressible, axisymmetric, Navier-Stocks equations with SST k-${\omega}$ turbulence model are solved using a fully implicit finite volume scheme. In order to simulate the shut-down process of the engine, NPR is varied from 100.0 to 10.0. It is observed that the separation point and Mach-disk strongly depend on the variation of NPR, and adjusting the step lead to significantly different characteristics in the lateral forces.

• Journal of fish pathology
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• v.35 no.2
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• pp.241-246
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• 2022

White spot syndrome virus (WSSV) is a prevalent and virulent pathogen affecting cultured whiteleg shrimp (Litopenaeus vannamei) in Korea. In this study, seven monoclonal antibodies (mAbs) (10A12, 16C3, 17G4, 21G5, 22C4, 23B6 and 24G6) were produced by using purified WSSV. The reactivity of these mAbs was analysed by Western blot (WB), indirect immunofluorescence (IIF), and lateral flow immunochromatographic assay (LFIA). WB analysis demonstrated that three mAbs (17G4, 22C4, and 23B6) reacted specifically to VP28 with an approximate molecular weight of 24 kDa, mAb 16C3 reacted with approximately 17 kDa. IIF analysis demonstrated specific fluorescence signals on gill tissues of WSSV-infected shrimp, with five mAbs (10A12, 16C3, 22C4, 23B6, and 24G6), pleopods from WSSV-infected shrimp were used for LFIA, where, two mAbs (21G5 and 22C4) exhibited positive reaction. In conclusion, it can be inferred that the mAbs usage and specificity depends on the nature of assay used for diagnosis.