• Title/Summary/Keyword: laser heat treatment

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Identification of Heat Stress-related Proteins and Low Molecular Weight HSP Expressed in Stem Tissues of Rice Plants by Proteomic Analysis (프로테옴 분석법에 의한 벼 줄기에서 발현하는 고온 스트레스 관련 단백질 및 저분자량 Heat Shock Protein의 분리 동정)

  • Lee, Dong-Gi;Kim, Kyung-Hee;Kim, Yong-Gu;Lee, Ki-Won;Lee, Sang-Hoon;Lee, Byung-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.2
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    • pp.99-106
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    • 2011
  • In order to investigate rice stem proteome in response to heat stress, rice plants were subjected to heat treatment at 42$^{\circ}C$ and total soluble proteins were extracted from stem tissues, and were fractionated with 15% PEG (poly ethylene glycol) and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). After staining of 2-DE gels, 46 of differentially expressed proteins were extracted, digested by trypsin, and subjected to matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Proteins were identified through database search by using peptide mass fingerprints. Among them, 10 proteins were successfully identified. Seven proteins were up- and 3 proteins were down-regulated, respectively. These proteins are involved in energy and metabolism, redox homeostasis, and mitochondrial small heat shock proteins. The identification of some novel proteins in the heat stress response provides new insights that can lead to a better understanding of the molecular basis of heat-sensitivity in plants, and also useful to molecular breeding of thermotolerant forage crops.

Effects of Low-Dose Aspirin Therapy on Thermoregulation in Firefighters

  • McEntire, Serina J.;Reis, Steven E.;Suman, Oscar E.;Hostler, David
    • Safety and Health at Work
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    • v.6 no.3
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    • pp.256-262
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    • 2015
  • Background: Heart attack is the most common cause of line-of-duty death in the fire service. Daily aspirin therapy is a preventative measure used to reduce the morbidity of heart attacks but may decrease the ability to dissipate heat by reducing skin blood flow. Methods: In this double-blind, placebo-controlled, crossover study, firefighters were randomized to receive 14 days of therapy (81-mg aspirin or placebo) before performing treadmill exercise in thermal-protective clothing in a hot room [$38.8{\pm}2.1^{\circ}C$, $24.9{\pm}9.1%$ relative humidity (RH)]. Three weeks without therapy was provided before crossing to the other arm. Firefighters completed a baseline skin blood-flow assessment via laser Doppler flowmetry; skin was heated to $44^{\circ}C$ to achieve maximal cutaneous vasodilation. Skin blood flow was measured before and after exercise in a hot room, and at 0 minutes, 10 minutes, 20 minutes, and 30 minutes of recovery under temperature conditions ($25.3{\pm}1.2^{\circ}C$, $40.3{\pm}13.7%\;RH$). Platelet clotting time was assessed before drug administration, and before and after exercise. Results: Fifteen firefighters completed the study. Aspirin increased clotting time before and after exercise compared with placebo (p = 0.003). There were no differences in absolute skin blood flow between groups (p = 0.35). Following exercise, cutaneous vascular conductance (CVC) was $85{\pm}42%$ of maximum in the aspirin and $76{\pm}37%$ in the placebo groups. The percentage of maximal CVC did not differ by treatment before or after recovery. Neither maximal core body temperature nor heart rate responses to exercise differed between trials. Conclusion: There were no differences in skin blood flow during uncompensable heat stress following exercise after aspirin or placebo therapy.

Application of 630-nm and 850-nm Light-emitting Diodes and Microcurrent to Accelerate Collagen and Elastin Deposition in Porcine Skin

  • Kwon, Tae-Rin;Moon, Dong Wook;Kim, Jungwook;Kim, Hyoung Jun;Lee, Seong Jae;Han, Yunhee;Dan, Hee Won;Chi, Sang Hoon;Seong, Hwan Mo;Kim, Hee Jung;Lim, Guei-Sam;Lee, Jungkwan
    • Medical Lasers
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    • v.10 no.2
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    • pp.96-105
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    • 2021
  • Background and Objectives Skin aging is reportedly associated with regulation in collagen and elastin synthesis. This study investigated the potential of combining light-emitting diode (LED) treatments using a 630-nm and 850-nm LED with simultaneous microcurrent application. Materials and Methods The dorsal skin of female pigs was treated with a home-use device. We examined the treatment effects using photography, thermocamera, microscopic pathology, and histological examination to determine the mechanism of action, efficacy, and safety of the procedure. A histological observation was performed using hematoxylin and eosin, Masson's trichrome, Victoria blue, and immunohistochemical staining. We also used the Sircol soluble collagen and elastin assay kit to measure the amounts of collagen and elastin in the porcine back skin tissue after 2 and 6 weeks. Results Evaluation by visual inspection and devices showed no skin damage or heat-induced injury at the treatment site. Histological staining revealed that accurate treatment of the targeted dermis layer effectively enhanced collagen and elastin deposition. Collagen type I, a protein defined by immunohistochemical staining, was overexpressed in the early stages of weeks 2 and 6. Combined therapy findings showed the superior capability of the 630-nm and 850-nm LED procedures to induce collagen; in contrast, elastin induction was more pronounced after microcurrent treatments. Conclusion The home-use LED device, comprising a combination of 630-nm and 850-nm LEDs and microcurrent, is safe and can be used as an adjunctive treatment for self-administered facial rejuvenation.

INTERGRANULAR CORROSION-RESISTANT STAINLESS STEEL BY GRAIN BOUNDARY ENGINEERING

  • Hiroyuki Kokawa;Masayuki Shimada;Wang, Zhan-Jie;Yutaka S. Sato
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.250-254
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    • 2002
  • Intergranular corrosion of austenitic stainless steels is a conventional and momentous problem during welding and high temperature use. One of the major reasons for such intergranular corrosion is so-called sensitization, i.e., chromium depletion due to chromium carbide precipitation at grain boundaries. Conventional methods for preventing sensitization of austenitic stainless steels include reduction of carbon content in the material, stabilization of carbon atoms as non-chromium carbides by the addition of titanium, niobium or zirconium, local solution-heat-treatment by laser beam, etc. These methods, however, are not without drawbacks. Recent grain boundary structure studies have demonstrated that grain boundary phenomena strongly depend on the crystallographic nature and atomic structure of the grain boundary, and that grain boundaries with coincidence site lattices are immune to intergranular corrosion. The concept of "grain boundary design and control", which involves a desirable grain boundary character distribution, has been developed as grain boundary engineering. The feasibility of grain boundary engineering has been demonstrated mainly by thermomechanical treatments. In the present study, a thermomechanical treatment was tried to improve the resistance to the sensitization by grain boundary engineering. A type 304 austenitic stainless steel was pre-strained and heat-treated, and then sensitized, varying the parameters (pre-strain, temperature, time, etc.) during the thermomechanical treatment. The grain boundary character distribution was examined by orientation imaging microscopy. The intergranular corrosion resistance was evaluated by electrochemical potentiokinetic reactivation and ferric sulfate-sulfuric acid tests. The sensitivity to intergranular corrosion was reduced by the thermomechanical treatment and indicated a minimum at a small roll-reduction. The frequency of coincidence-site-lattice boundaries indicated a maximum at a small strain. The ferric sulfate-sulfuric acid test showed much smaller corrosion rate in the thermomechanically-treated specimen than in the base material. An excellent intergranular corrosion resistance was obtained by a small strain annealing at a relatively low temperature for long time. The optimum parameters created a uniform distribution of a high frequency of coincidence site lattice boundaries in the specimen where corrosive random boundaries were isolated. The results suggest that the thermomechanical treatment can introduce low energy segments in the grain boundary network by annealing twins and can arrest the percolation of intergranular corrosion from the surface.

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Effect of strontium ions on K2O-TiO2-SiO2 glass soaked in molten Sr(NO3)2 (Sr(NO3)2 용융염에서 이온교환이 K2O-TiO2-SiO2 glass에 미치는 영향)

  • Park, No-Hyung;Yoo, Eun-Sung;Lee, Hoi-Kwan;Huh, Hoon
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.21 no.5
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    • pp.214-217
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    • 2011
  • A fresnoite glass-ceramics has been investigating considerably because of the second order nonlinearity as well as its permanent optical nonlinearity. However, the chemical compositions of transparent glass-ceramics examined in the previous studies have been limited to adjust the composition close to fresnoite stoichiometrically. In this study, to investigate the effect of strontium ions in glass-ceramics on second order nonlinearity, $K_2O-TiO_2-SiO_2$ (KTS) glasses soaked in molten $Sr(NO_3)_2$ were prepared. Crystalline phases of the soaked glasses were measured by X-ray diffraction (XRD) as soaking times and additional heat treatment. The exchange of strontium ions into the glass was verified by Field Emission Scanning Electron Microscope (FE-SEM). And the phenomena of second order harmonic generation (SHG) was observed using Nd-YAG laser.

Fabrication and Measurement of Optical Waveguide using Multi Quantum Well Intermixing (다중양자우물구조의 상호섞임을 이용한 광도파로의 제작 및 측정)

  • Yeo, Deok-Ho;Yoon, Kyung-Hun;Kim, Sung-June
    • Journal of the Korean Institute of Telematics and Electronics D
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    • v.36D no.7
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    • pp.50-55
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    • 1999
  • We have fabricated optical waveguide which utilizes intermixing of InGaAs/InGaAsP multi quantum well separate confinement heterostructure. The waveguide was fabricated by reactive ion etching technique using $CH_4/H_2$ gas mixture, and the width and depth of the waveguide ware $5{\mu}m$ and $1.2{\mu}m$, respectively. The propagation loss of the waveguide was measured by Fabry-Perot interference phenomena using tunable laser. For the waveguide after $800^{\circ}C$, 30s heat treatment, the measured loss was 3.76dB/cm and 3.95dB/cm for TE and TM mode, respectively. This value is very small compared to other waveguide made by IFVD technique. Hence, this technique can applied to integration of waveguide and electronic devices.

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TENSILE STRENGTH OF LASER WELDED-TITANIUM AND GOLD ALLOYS (티타늄과 금합금의 레이저 용접부의 인장강도)

  • Song, Yun-Gwan;Ha, Il-Soo;Song, Kwang-Yeob
    • The Journal of Korean Academy of Prosthodontics
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    • v.38 no.2
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    • pp.200-213
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    • 2000
  • Lasers have given dentistry a new rapid, economic, and accurate technique for metal joining. Although laser welding has been recommended as an accurate technique, there are some limitations with this technique. For example, the two joining surfaces must have a tight-fitting contact, which may be difficult to achieve in some situations. The tensile samples used for this study were made from a custom-made pure titanium and type III gold alloy plates. 27 of 33 specimens were sectioned perpendicular to their long axis with a carborundum disk and water coolant. Six specimens remained and served as the control group. A group of 6 specimens was posed as butt joints in custom parallel positioning device with a feeler gauge at each of three gaps : 0.00, 0.25. and 0.50mm. All specimens were then machined to produce a uniform cross-sectional dimension, none of the specimens was subjected to any subsequent form of heat treatment. Scanning electron microscopy was performed on representative tested specimens at fractured surfaces in both the parent metal and the weld. Vickers hardness was measured at the center of the welds with a micropenetrometer using a force of 300gm for 15 seconds. Measurement was made at approximately $200{\mu}m\;and\;500{\mu}m$ deep from each surface. One-way analysis of variance (ANOVA) and Scheffe's test was calculated to detect differences between groups. The purpose of this study is to compare the strength and properties of the joint achieved at various butt Joint gaps by the laser welding of type III gold alloy and pure titanium tensile specimens in an argon atmosphere. The results of this study were as follows : 1. When indexing and welding pure titanium, there was no decrease in ultimate tensile strength as compared with the unsectioned alloys for indexing gaps of 0.00 to 0.50mm, although with increasing gap size may come increased distortion (p>0.05). 2. When indexing and welding type III gold alloy, there were significant differences in ultimate tensile strength among groups with weld gaps of 0.00mm, 0.25 and 0.50mm, and the control group. Group with butt contact without weld gap demonstrated a significant higher ultimate tensile strength than groups with weld gaps of 0.25 and 0.50mm (p<0.05). 3. When indexing and welding the different metal combination of type III gold alloy and pure titanium, there were significant differences in ultimate tensile strength between groups with weld gaps of 0.00, 0.25, and 0.50mm. However, the mechanical properties of the welded joint would become too brittle to be acceptable clinically (p<0.05). 4. The presence of large pores in the laser welded joint appears to be the most important factor in controlling the tensile strength of the weld in both pure titanium and type III gold alloy.

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Investigation of Planar Optical Waveguide Formed by MeV $He^{+}$ Ion-Implantation into NaEr(WO$_4$)$_2$ Crystal

  • Feng Chen;Wang, Xue-Lin;Wang, Ke-Ming;Cheng, Zhen-Xiang;Chen, Huan-Chu;Shen, Ding-Yu
    • Journal of Korean Vacuum Science & Technology
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    • v.6 no.2
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    • pp.97-100
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    • 2002
  • NaEr(WO$_4$)$_2$ is a new laser material. The planar optical waveguide was formed in NaEr(WO$_4$)$_2$ crystal by 2.6 MeV He$^{+}$ ion implantation at doses of 1.0-1.5 $\times$ 10$^{16}$ ions/cm$^2$ at room temperature. The effective refractive indices of the dark modes were measured using the prism coupling method. foul n modes and five TM modes were observed in the waveguide. The refractive index profiles were analyzed using the reflectivity calculation method (RCM). The influence of heat treatment at moderate temperature on the refractive index profiles of the waveguide was also investigated. We used the TRIM'98 (Transport of ton in Matter) code to simulate the damage profile in the NaEr(WO$_4$) crystal by 2.6 MeV He$^{+}$ion implantation which is helpful for a better understanding of the waveguide formation.ion.

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Implementation of process and surface inspection system for semiconductor wafer stress measurement (반도체 웨이퍼의 스트레스 측정을 위한 공정 및 표면 검사시스템 구현)

  • Cho, Tae-Ik;Oh, Do-Chang
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.8
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    • pp.11-16
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    • 2008
  • In this paper, firstly we made of the rapid thermal processor equipment with the specifically useful structure to measure wafer stress. Secondly we made of the laser interferometry to inspect the wafer surface curvature based on the large deformation theory. And then the wafer surface fringe image was obtained by experiment, and the full field stress distribution of wafer surface comes into view by signal processing with thining and pitch mapping. After wafer was ground by 1mm and polished from the back side to get easily deformation, and it was heated by three to four times thermal treatments at about 1000 degree temperature. Finally the severe deformation between wafer before and after the heat treatment was shown.

Study of the capability of metallic coils immersed with protein solution as embolic masterials (색전용 금속 코일 개발과 단백질 처리 후 혈전성에 대한 연구)

  • Lee, Y.S.;Kim, T.S.;Kim, H.C.;Shin, K.M.;Park, J.H.
    • Proceedings of the KOSOMBE Conference
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    • v.1997 no.11
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    • pp.347-349
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    • 1997
  • In this study, we designed a vascular occlusion device based on the principle that slowing blood flow would induce thrombosis. Tungsten, stainless steel and platinum were tested for finding a good embolic metallic coil. The primary coil and the second coil were done with heat treatment or different time. The pitch distance and the shape retention capability of second coil were characterized by SEM. To increase thrombogenicity, we tried to treat different proteins on steel coils: thrombin, gelatin and both gelatin and thrombin. To verify protein materials treated on coils, the surface of coil treated with different proteins were characterized using Laser Raman Spectrophotometer. After observation of embolic coils, the peptides bonds on theirs' surface were found. In order to compare the thrombogenicity of different embolic agents, we measured whole blood clotting time.

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