Medical Lasers

  • 제10권2호
  • /
  • Pages.96-105
  • /
  • 2021
  • /
  • 2287-8300(pISSN)
  • /
  • 2288-0224(eISSN)
대한의학레이저학회 (Korean Society for Laser Medicine and Surgery)
권호 표지
DOI QR코드

DOI QR Code

Application of 630-nm and 850-nm Light-emitting Diodes and Microcurrent to Accelerate Collagen and Elastin Deposition in Porcine Skin

  • 투고 : 2021.05.24
  • 심사 : 2021.05.27
  • 발행 : 2021.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

Background and Objectives Skin aging is reportedly associated with regulation in collagen and elastin synthesis. This study investigated the potential of combining light-emitting diode (LED) treatments using a 630-nm and 850-nm LED with simultaneous microcurrent application. Materials and Methods The dorsal skin of female pigs was treated with a home-use device. We examined the treatment effects using photography, thermocamera, microscopic pathology, and histological examination to determine the mechanism of action, efficacy, and safety of the procedure. A histological observation was performed using hematoxylin and eosin, Masson's trichrome, Victoria blue, and immunohistochemical staining. We also used the Sircol soluble collagen and elastin assay kit to measure the amounts of collagen and elastin in the porcine back skin tissue after 2 and 6 weeks. Results Evaluation by visual inspection and devices showed no skin damage or heat-induced injury at the treatment site. Histological staining revealed that accurate treatment of the targeted dermis layer effectively enhanced collagen and elastin deposition. Collagen type I, a protein defined by immunohistochemical staining, was overexpressed in the early stages of weeks 2 and 6. Combined therapy findings showed the superior capability of the 630-nm and 850-nm LED procedures to induce collagen; in contrast, elastin induction was more pronounced after microcurrent treatments. Conclusion The home-use LED device, comprising a combination of 630-nm and 850-nm LEDs and microcurrent, is safe and can be used as an adjunctive treatment for self-administered facial rejuvenation.

키워드

과제정보

We thank CRONEX (Hwasung, Korea) for its support in this study.

참고문헌

  1. Benedetto AV. What's new in cosmetic dermatology. Dermatol Clin 2019;37:117-28. https://doi.org/10.1016/j.det.2018.08.002
  2. Clarke LH, Korotchenko A. Aging and the body: a review. Can J Aging 2011;30:495-510. https://doi.org/10.1017/S0714980811000274
  3. Wang B. Photoaging: a review of current concepts of pathogenesis. J Cutan Med Surg 2011;15 Suppl 1:S374-7.
  4. Barolet D, Roberge CJ, Auger FA, Boucher A, Germain L. Regulation of skin collagen metabolism in vitro using a pulsed 660 nm LED light source: clinical correlation with a single-blinded study. J Invest Dermatol 2009;129:2751-9. https://doi.org/10.1038/jid.2009.186
  5. Li WH, Seo I, Kim B, Fassih A, Southall MD, Parsa R. Low-level red plus near infrared lights combination induces expressions of collagen and elastin in human skin in vitro. Int J Cosmet Sci. In press 2021.
  6. Smith RB. Microcurrent therapies: emerging theories of physiological information processing. NeuroRehabilitation 2002;17:3-7. https://doi.org/10.3233/nre-2002-17102
  7. He B. Bioelectricity of living tissue. IEEE Eng Med Biol Mag 1998;17:72, 117.
  8. Gold MH, Biron J, Levi L, Sensing W. Safety, efficacy, and usage compliance of home-use device utilizing RF and light energies for treating periorbital wrinkles. J Cosmet Dermatol 2017;16:95-102. https://doi.org/10.1111/jocd.12299
  9. Wei JCJ, Edwards GA, Martin DJ, Huang H, Crichton ML, Kendall MAF. Allometric scaling of skin thickness, elasticity, viscoelasticity to mass for micro-medical device translation: from mice, rats, rabbits, pigs to humans. Sci Rep 2017;7:15885. https://doi.org/10.1038/s41598-017-15830-7
  10. Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods 2012;9:671-5. https://doi.org/10.1038/nmeth.2089
  11. Wang JV, Saedi N, Geronemus RG. Differentiation in a market of imitation: the evolving world of aesthetic dermatology. J Cosmet Dermatol 2020;19:2987-9. https://doi.org/10.1111/jocd.13645
  12. Sherber NS, Rad AN. Future directions in facial rejuvenation. Facial Plast Surg 2014;30:72-5. https://doi.org/10.1055/s-0033-1363764
  13. Humphrey S, Beleznay K, Fitzgerald R. Combination therapy in midfacial rejuvenation. Dermatol Surg 2016;42 Suppl 2:S83-8. https://doi.org/10.1097/DSS.0000000000000747
  14. Nestor MS. Combination therapy in clinical and cosmetic dermatology: the marriage of device and drug. J Drugs Dermatol 2004;3(5 Suppl):S4-11.
  15. Coleman KM, Pozner J. Combination therapy for rejuvenation of the outer thigh and buttock: a review and our experience. Dermatol Surg 2016;42 Suppl 2:S124-30. https://doi.org/10.1097/DSS.0000000000000752
  16. Collawn SS. Combination therapy: utilization of CO2 and Erbium:YAG lasers for skin resurfacing. Ann Plast Surg 1999;42:21-6. https://doi.org/10.1097/00000637-199901000-00004
  17. Omi T, Kawana S, Sato S, Naito Z. Histological evidence for skin rejuvenation using a combination of pneumatic energy, broadband light, and growth factor therapy. J Cosmet Laser Ther 2010;12:222-6. https://doi.org/10.3109/14764172.2010.502459
  18. Shek SY, Yeung CK, Chan JC, Chan HH. The efficacy of a combination non-thermal focused ultrasound and radiofrequency device for noninvasive body contouring in Asians. Lasers Surg Med 2016;48:203-7. https://doi.org/10.1002/lsm.22406
  19. Lin YL, Moolenaar H, van Weeren PR, van de Lest CH. Effect of microcurrent electrical tissue stimulation on equine tenocytes in culture. Am J Vet Res 2006;67:271-6. https://doi.org/10.2460/ajvr.67.2.271
  20. Poltawski L, Johnson M, Watson T. Microcurrent therapy in the management of chronic tennis elbow: pilot studies to optimize parameters. Physiother Res Int 2012;17:157-66. https://doi.org/10.1002/pri.526
  21. Hamblin MR. Mechanisms and mitochondrial redox signaling in photobiomodulation. Photochem Photobiol 2018;94:199-212. https://doi.org/10.1111/php.12864
  22. Tatmatsu-Rocha JC, Tim CR, Avo L, Bernardes-Filho R, Brassolatti P, Kido HW, et al. Mitochondrial dynamics (fission and fusion) and collagen production in a rat model of diabetic wound healing treated by photobiomodulation: comparison of 904 nm laser and 850 nm light-emitting diode (LED). J Photochem Photobiol B 2018;187:41-7. https://doi.org/10.1016/j.jphotobiol.2018.07.032
  23. Zhang Y, Song S, Fong CC, Tsang CH, Yang Z, Yang M. cDNA microarray analysis of gene expression profiles in human fibroblast cells irradiated with red light. J Invest Dermatol 2003;120:849-57. https://doi.org/10.1046/j.1523-1747.2003.12133.x