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http://dx.doi.org/10.5333/KGFS.2011.31.2.99

Identification of Heat Stress-related Proteins and Low Molecular Weight HSP Expressed in Stem Tissues of Rice Plants by Proteomic Analysis  

Lee, Dong-Gi (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University)
Kim, Kyung-Hee (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University)
Kim, Yong-Gu (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University)
Lee, Ki-Won (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University)
Lee, Sang-Hoon (National Institute of Animal Science, RDA)
Lee, Byung-Hyun (Dept. of Animal Biosciences, IALS, PMBBRC, Division of Applied Life Sciences (BK21), Gyeongsang National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.31, no.2, 2011 , pp. 99-106 More about this Journal
Abstract
In order to investigate rice stem proteome in response to heat stress, rice plants were subjected to heat treatment at 42$^{\circ}C$ and total soluble proteins were extracted from stem tissues, and were fractionated with 15% PEG (poly ethylene glycol) and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). After staining of 2-DE gels, 46 of differentially expressed proteins were extracted, digested by trypsin, and subjected to matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Proteins were identified through database search by using peptide mass fingerprints. Among them, 10 proteins were successfully identified. Seven proteins were up- and 3 proteins were down-regulated, respectively. These proteins are involved in energy and metabolism, redox homeostasis, and mitochondrial small heat shock proteins. The identification of some novel proteins in the heat stress response provides new insights that can lead to a better understanding of the molecular basis of heat-sensitivity in plants, and also useful to molecular breeding of thermotolerant forage crops.
Keywords
Heat stress; Molecular breeding; MALDI-TOF; Proteome;
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