• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.96-96
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• 2020

Soybean seeds are important sources of non-nutritive health promoting metabolites. The contents of these metabolites are affected by both genetic and environmental factors. In the present study, the contents of five common isoflavones including daidzin, genistin, glycitin, malonyldaidzin, and malonylgenistin were analyzed in 72 soybeans of different seed coat colors, diversity, and of different origins including China (22), Japan (9), USA (12), India (4), and Korea (25). The average total isoflavone content (TIC) was maximum in Indian soybeans (3302.36 ㎍/g) and minimum in Chinese landraces (1214.95 ㎍/g). The Korean landraces had higher average TIC (2148.05 ㎍/g) than the USA genetic materials (1580.23 ㎍/g) and Japanese landraces (1485.99 ㎍/g). The content of malonylgenistin was in the range of 54.31 - 2385.68 ㎍/g in the entire population, and was the most abundant isoflavone irrespective of origin although there was content variation among individual soybeans. Besides, glycitin was the least concentrated isoflavone, and its content ranged from 0.00 to 79.79 ㎍/g. With respect to seed coat color, green soybeans from all countries displayed the maximum malonylgenistin and TIC contents. Exceptions were those of Korean and Indian origins where black and yellowish-green soybeans presented the highest malonylgenistin and TIC contents, respectively. In multivariate analysis, 92.72% of the variance was explained by the first two principal components, and the soybeans were grouped in to three clusters based on isoflavone contents. Overall, our findings signify the importance of seed coat color and origin as discriminant parameters, and provide wide spectrum of routes for breeding soybean cultivars.

• 한국작물학회지
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• 제52권1호
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• pp.81-88
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• 2007

본 연구는 농진청 종자은행에 보존된 한국, 중국 및 일본 재래종 콩과 한국 야생콩의 SSR profile 작성과 그들의 유전적 구조 해석을 위해 9개의 SSR 마커에 의해 분석되었다. 1. DNA profiling은 유전자좌별로 2,855(Satt458)점$\sim$4,368(Satt197)점이 분석되어 35,655건이 데이터베이스화되었다. 2. 총 대립인자수는 267개였고 유전자좌당 평균 29.6개의 높은 다형성을 나타냈다. 유전자좌별 대립인자 수는 21개(Satt532 및 Satt141)부터 58개(Sat_074)까지 나타났다. 자원내력별 대립인자수는 한국 야생콩에서 196개로 가장 많은 것으로 나타난 반면, 일본 재래종 콩에서는 가장 적은 115개로 나타났다. 3. 집단에 따른 유전자좌별 대립인자의 범위로 한국 재래종 콩이 가장 많은 5개의 유전자좌(Sat_074, Satt141, Satt286, Satt545, Satt458)에서 다음으로는 한국 야생콩이 4개의 유전자좌(Satt187, Satt532, Satt245, Satt197)에서 가장 넓은 것으로 나타났다. 그러나 대립 인자수면에서는 한국 재래종 콩이 5개의 유전자좌(Sat_074, Satt141, Satt197, Satt545, Satt458)에서 가장 많은 대립인자수를 나타냈고, 한국 야생콩은 나머지 4개의 유전자좌(Satt187, Satt532, Satt245, Satt286)에서 가장 많은 대립인자수를 나타냈다. 4. 대립인자 분포에 있어 전체적으로 한국 야생콩 집단은 재래종 집단들에 비해 고른 분포를 나타냈고 대립인자의 크기가 큰쪽(high ladder)에서보다 작은쪽(low ladder)에서 높은 분포를 나타냈다. 5. 재래종 집단들 간에 대립인자 분포를 살펴보면, 한국 집단은 Satt286(202 bp, 232 bp)에서, 중국집단은 Satt197(171 bp)와 Satt458(173 bp)에서 그리고 일본집단은 Sat_074(244 bp)와 Satt458(170 bp)에서 매우 높은 것으로 나타났다.

• 생명과학회지
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• 제18권4호
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• pp.435-440
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• 2008

RAPD 분석을 이용하여 지황 육성 계통과 지역 수집종 들을 구분할 수 있는 분자표지자를 선발하고, 집단 간, 집단 내 유전적 다양성을 평가하기 위하여 본 실험을 수행하였다. 총 20개의 임의 primer를 이용하여 PCR 한결과, 육성 계통과 수집종 들을 구별할 수 있는 OPA-1 등 10개의 재현성과 다형성이 좋은 프라이머 들을 선발하였다. 특히 OPA-10, OPA-11 및 OPA-19는 고려지황과 지황1호를 다른 계통 및 수집종 들과 구별할 수 있었으며, 이들 프라이머를 이용하여 0.9 kb, 1.2 kb, 1.3 kb 및 1.4 kb등의 육성계통 특이적인 DNA 밴드들을 확보할 수 있었다. 한편 이들의 결과를 토대로 통계처리에 의한 유전분석 결과, 고려지황, 지황1호 및 일본지황은 집단 내 유사도가 높아 다른 집단들과 구별되었다. 결론적으로, RAPD 분석을 통한 결과는 지황의 유전적 다양성 이해와 특정 계통을 다른 계통 및 수집종 들과 구분할 수 있는 방법으로 이용될 수 있다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2003년도 추계 학술발표회 요지
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• pp.222-223
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• 2003

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1997년도 춘계학술발표회 및 학술연구발표요지
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• pp.31-31
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• 1997

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1997년도 추계정기총회 및 학술발표대회
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• pp.55-55
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• 1997

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 제7차 국제 심포지움(생약자원개발에 관한연구) 및 추계정기 학술발표회 초록집
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• pp.29-29
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• 2000

• Plant Resources
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• 제1권2호
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• pp.81-91
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• 1998

Fifty-nine Korean soybean (Glycine max L. Merr.) landrace accessions were tested for genotype fingerprinting, differentiation and association between morphological traits and SSR profile. Using 8 SSR loci, 59 varieties were divided into 55 groups, and only 4 pairs of varieties were not uniquely identified. The resolving power of SSR for soybean genotyping was much higher than that of the morphological traits that were studied. Identification efficiency also differed among SSR loci. Those loci with higher numbers of alleles distinguished varieties more effectively. Genetic differentiation values of the soybean landraces varied from 0.57 to 0.82 with a mean of 0.68. The number of alleles detected by the 8 loci ranged from 3 to 8. and the effective number of alleles ranged from 2.3 to 5.1. In a study of the association of SSR alleles with morphological traits, some alleles seemed to be related with some specific morphological traits. Comparison of two kinds of dendrograms which were derived from SSR markers and quantitative traits indicated that the dendrograms were not consistent. Considering the correlation between single SSR locus and qualitative traits governed by major genes, the data suggest that alleles of microsatellite loci be more closely related to some traits determined by major genes than those determined by minor genes.

• 한국작물학회지
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• 제50권2호
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• pp.142-146
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• 2005

Thirty-two germplasms of Korean adlay landraces were examined to analyse the genetic relationship through the amplified fragment length polymorphism (AFLP) approach. Total number of AFLP products generated by 12 selective primer combinations was 882. The number of polymorphic fragments by each primer combination greatly varied from 4 to 51 with a mean of 20.3, bands visible on the polyacrylamide gel. A genetic similarity coefficient was used for cluster analysis following UPGMA (unweighted pair grouping method of averages) method. The resulting clusters were represented in the form of a dendrogram. The clustering was not tight in the dendrogram. There was generally no clear grouping of the adlay according to the geographic regions in which germplasms were collected. The present AFLP analysis imply that although Korean adlay displayed a larger amount of AFLP variation within germplasms, the variation was shown independently without reflecting a clinal variation. This study demonstrated that AFLP method can be used to examine the genetic relationships among different germplasms of adlay.

• Journal of Crop Science and Biotechnology
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• 제11권3호
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• pp.157-162
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• 2008

A total of 2,765 accessions were used as the initial set having both seed coat color and 100-seed weight data. As a result of molecular profiling using six SSR markers followed by stratification based on their usages, 335 accessions(12.1%) were selected by clustering based on UPGMA. Since 75 out of 335 accessions were mixed in phenotypic traits as a result of characterization, 260 accessions were finally set as a core set. This core set revealed nearly the same diversity compared with the other results on morphological traits of Korean soybean landraces. In total, 115 alleles(19.2 alleles per locus) were detected in the initial set and 79 alleles(13.2 alleles per locus) were detected in the core set. All 30 major alleles were present in the initial set and in the core set as well. In allele coverage, the core set was 71.4% of the initial set. These comparisons of number of alleles, gene diversity and coverage indicated that the core set represented the entire set well.