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Genetic Relationships among Korean Adlay, Coix lachryma-jobi L., Landraces Based on AFLPs  

Moon Jung-Hun (Department of Plant Biotechnology, Dongguk University)
Jang Jung Hee (Gyeonggido ARES)
Park Jung Soo (Gyeonggido ARES)
Kim Sung Kee (Gyeonggido ARES)
Lee Kyung-Jun (Department of Plant Biotechnology, Dongguk University)
Lee Sang-Kyu (Department of Plant Biotechnology, Dongguk University)
Kim Kyung-Hee (Department of Plant Biotechnology, Dongguk University)
Lee Byung-Moo (Department of Plant Biotechnology, Dongguk University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.50, no.2, 2005 , pp. 142-146 More about this Journal
Abstract
Thirty-two germplasms of Korean adlay landraces were examined to analyse the genetic relationship through the amplified fragment length polymorphism (AFLP) approach. Total number of AFLP products generated by 12 selective primer combinations was 882. The number of polymorphic fragments by each primer combination greatly varied from 4 to 51 with a mean of 20.3, bands visible on the polyacrylamide gel. A genetic similarity coefficient was used for cluster analysis following UPGMA (unweighted pair grouping method of averages) method. The resulting clusters were represented in the form of a dendrogram. The clustering was not tight in the dendrogram. There was generally no clear grouping of the adlay according to the geographic regions in which germplasms were collected. The present AFLP analysis imply that although Korean adlay displayed a larger amount of AFLP variation within germplasms, the variation was shown independently without reflecting a clinal variation. This study demonstrated that AFLP method can be used to examine the genetic relationships among different germplasms of adlay.
Keywords
adlay; germplasm; AFLP; genetic relationship;
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