• 제목/요약/키워드: laboratory method

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실험실의 안전성 확보에 관한 연구 (- A Study on the Improvement of Safety in the Laboratory -)

  • 지철구;권혁일
    • 대한안전경영과학회지
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    • 제6권4호
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    • pp.47-59
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    • 2004
  • Safety in the laboratory has been a growing interest due to recent recurrences of the fatal accidents such as physical or chemical explosions. It is not easy to determine the extent to what the industrial safety and health law should be applicable to the laboratory. Most laboratory workers are not sufficiently trained and recognized for the generic features of safety and health. The actual conditions of safety and health in the laboratory are not familar with laboratory workers. Safety and health in the laboratory is unfortunately in the dead ground. Therefore, it is most imperative to secure safety in the laboratory. This study proposes a method to improve safety in the laboratory.

실험실 안전성 확보에 관한 연구 (- A Study on the Improvement of Safety in the Laboratory -)

  • 지철구;권혁일
    • 대한안전경영과학회:학술대회논문집
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    • 대한안전경영과학회 2004년도 추계학술대회
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    • pp.289-299
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    • 2004
  • Safety in the laboratory has been a growing interest due to recent recurrences of the fatal accidents such as physical or chemical explosions. It is not easy to determine, to what extents the industrial safety and health law should apply the laboratory. Most laboratory workers are not sufficiently trained and recognized for the generic features of safety and health. The actual conditions of safety and health in the laboratory are not appropriate for laboratory workers. Safety and health in the laboratory is unfortunately in the dead ground. Therefore, it is most imperative to secure safety in the laboratory. This study proposes a method to improve safety in the laboratory.

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Effects of particle size and adaptation duration on the digestible and metabolizable energy contents and digestibility of various chemical constituents in wheat for finishing pigs determined by the direct or indirect method

  • Fan, Yuanfang;Guo, Panpan;Yang, Yuyuan;Xia, Tian;Liu, Ling;Ma, Yongxi
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권4호
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    • pp.554-561
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    • 2017
  • Objective: This experiment was conducted as a $3{\times}2{\times}2$ factorial design to examine the effects of particle size (mean particle size of 331, 640, or $862{\mu}m$), evaluation method (direct vs indirect method) and adaptation duration (7 or 26 days) on the energy content and the apparent total tract digestibility (ATTD) of various chemical components in wheat when fed to finishing pigs. Methods: Forty-two barrows ($Duroc{\times}Landrace{\times}Yorkshire$) with an initial body weight of $63.0{\pm}0.8kg$ were individually placed in metabolic cages and randomly allotted to 1 of 7 diets with 6 pigs fed each diet. For the indirect method, the pigs were fed either a corn-soybean meal based basal diet or diets in which 38.94% of the basal diet was substituted by wheat of the different particle sizes. In the direct method, the diets contained 97.34% wheat with the different particle sizes. For both the direct and indirect methods, the pigs were adapted to their diets for either 7 or 26 days. Results: A reduction in particle size linearly increased the digestible energy (DE) and metabolizable energy (ME) contents as well as the ATTD of gross energy, crude protein, organic matter, ether extract (EE) and acid detergent fiber (ADF) (p<0.05), and had a trend to increase the ATTD of dry matter of wheat (p = 0.084). The DE, ME contents, and ATTD of gross energy, crude protein, dry matter and organic matter were higher (p<0.05) when determined by the direct method, but the ATTD of ADF, EE, and neutral detergent fiber were higher when determined by the indirect method (p<0.05). Prolongation of the adaption duration decreased the ATTD of neutral detergent fiber (p<0.05) and had a trend to increase the ATTD of EE (p = 0.061). There were no interactions between particle size and the duration of the adaptation duration. The ATTD of EE in wheat was influenced by a trend of interaction between method and adaptation duration (p = 0.074). The ATTD of ADF and EE in wheat was influenced by an interaction between evaluation method and wheat particle size such that there were linear equations (p<0.01) about ATTD of ADF and EE when determined by the direct method but quadratic equations (p = 0.073 and p = 0.088, respectively) about ATTD of ADF and EE when determined by the indirect method. Conclusion: Decreasing particle size can improve the DE and ME contents of wheat; both of the direct and indirect methods of evaluation are suitable for evaluating the DE and ME contents of wheat with different particle sizes; and an adaptation duration of 7 d is sufficient to evaluate DE and ME contents of wheat in finishing pigs.

Effective Screening Method for Viviparous Germination of Rice

  • Ju, Young-Cheoul;Han, Sang-Wook;Park, Joong-Soo;Park, Kyeong-Yeol
    • 한국작물학회지
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    • 제45권2호
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    • pp.103-107
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    • 2000
  • The viviparity of 28 rice varieties was tested at 25 days after heading(DAH), 35DAH, and 45DAH in the laboratory and field condition for 12 days. The incubation temperature was 20/l$0^{\circ}C$ (day/night), 25/15$^{\circ}$C$ and 30/20$^{\circ}$C$ in the laboratory test, and under field water conditions in the field test. The biggest varietal difference of viviparity was found in the laboratory test when examined at 45DAH with the 6-day incubation under 25/15$^{\circ}$C$ . At this conditions the mean viviparous ratio was 32.1 % with the range of 53.9 and the variance of 259.5. In the field test, the significant varietal difference in the viviparity was also found in the lodging treatment at 45 DAH for 6 days. Correlation coefficient analysis between the field and laboratory tests was highly significant from 4 days after incubation at 45 DAH and after 6-day incubation at 35 DAH, and correlation coefficient was higher as incubation days in the laboratory and submerged days under field water became longer. Considering the correlation between the field and laboratory tests, varietal difference of viviparity and convenience of testing, the laboratory test at 45 DAH for 6-day incubation under 25/15$^{\circ}$C$ was the most efficient evaluation method for the viviparity of rice cultivar.

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Antimicrobial activity of Mongolian medicinal plants

  • Gonchig, Enkhmaa;Erdenebat, Sarnaizul;Togtoo, Ouyntsetseg;Bataa, Sukhkhuu;Gendaram, Odontuya;Kim, Young-Sup;Ryu, Shi-Yong
    • Natural Product Sciences
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    • 제14권1호
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    • pp.32-36
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    • 2008
  • The antimicrobial activity of seventy five ethanol extracts obtained from 67 different kinds of plant species of the Mongolian flora were evaluated by means of the disc diffusion method against five species of microorganisms, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Micrococcus luteus and Pseudomonas aeruginosa. Among the plant extracts examined, 34 kinds of extracts demonstrated significant antibacterial activity against one or more species of microorganisms, respectively. Especially, the root extract of Paeonia anomala, the whole herb extract of Myricaria alopecuroides, the whole herb extract of comarum zalesovianum, the whole herb extract of Agrimonia pilosa and some other plant extracts demonstrated a particularly potent antimicrobial activity. The ethylacetate fractions obtained from the whole herb extract of Myricaria alopecuroides and from those of Sedum aizoon, Paeonia anomala, Sedum hybridum and Dasiphora fruticosa exhibited a particularly potent antibacterial activity especially against Staphylococcus aureus and Micrococcus luteus.

Synthesis of CdS Nanocrystals with Different Shapes via a Colloidal Method

  • Bai, Jie;Liu, Changsong;Niu, Jinzhong;Wang, Hongzhe;Xu, Shasha;Shen, Huaibin;Li, Lin Song
    • Bulletin of the Korean Chemical Society
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    • 제35권2호
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    • pp.397-400
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    • 2014
  • Size- and shape-controlled monodisperse wurtzite structured CdS nanorods have been successfully synthesized using a facile solution-based colloidal method. Depending on the control of injection/growth temperatures and the variation of Cd-to-S molar ratios, the morphology of the CdS nanocrystals (NCs) can be adjusted into bullet-like, rod-like, and dot-like shapes. X-ray diffraction (XRD), transition electron microscopy (TEM), and absorption spectroscopy were used to characterize the structure, morphology, and optical properties of as-synthesized CdS NCs. It was found that uniform CdS nanorods could be successfully synthesized when the injection and growth temperatures were very high (> $360^{\circ}C$). The aspect ratios of different shaped (bullet-like or rod-like) CdS NCs could be controlled by simply adjusting the molar ratios between Cd and S.

Coulter® LH 750TM에 의한 백혈구 감별계산 및 Suspect Flags의 평가 (Evaluation of Coulter® LH 750TM on Leukocyte Differential Count and Suspect Flags)

  • 홍승복;최종태;이성규;김현수
    • 대한임상검사과학회지
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    • 제37권3호
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    • pp.143-148
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    • 2005
  • Automatic hematology analyzers provide the leukocyte differential count and the useful flags of the various hematological parameters. We compared LH 750 (Beckman Coulter Co., Miami, FL, USA) with manual method on differential leukocyte counts and evaluated the usefulness of the suspect flags, provided by this instrument. The comparison of leukocyte differential counts between two methods showed good correlation coefficient (r), which were 0.95 (neutrophil), 0.92 (lymphocyte), 0.82 (monocyte) and 0.95 (eosinophil). The frequency of the total flags displayed on LH 750 were 15.5%, which included immature granulocyte/left shift 63.5%, nucleated RBC 14.6%, platelet clumps 13.1%, variant lymphocyte 50% and blast 16.6%. This instrument showed higher positive predictive value in the flags such as platelet clumps 68.8%, immature granulocyte/left shift 61.5%, nucleated RBC 27.3%, variant lymphocyte 50% and blast 16.7%. In this study, the leukocyte differential counts of LH 750 showed good correlation with manual method and the suspect flags also showed a good performance for applying the criteria of re-examination in the clinical laboratory.

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Target alignment method of inertial confinement fusion facility based on position estimation

  • Lin, Weiheng;Zhu, Jianqiang;Liu, Zhigang;Pang, Xiangyang;Zhou, Yang;Cui, Wenhui;Dong, Ziming
    • Nuclear Engineering and Technology
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    • 제54권10호
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    • pp.3703-3716
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    • 2022
  • Target alignment technology is one of the most critical technologies in laser fusion experiments and is an important technology related to the success of laser fusion experiments. In this study, by combining the open-loop and closed-loop errors of the target alignment, the Kalman state observer is used to estimate the position of the target, which improves the observation precision of the target alignment. Then the optimized result is used to guide the alignment of the target. This method can greatly optimize the target alignment error and reduce uncertainty. With the improvement of the target alignment precision, it will greatly improve the reliability and repeatability of the experiments' results, thereby improving the success rate of the experiments.

Sea fog detection near Korea peninsula by using GMS-5 Satellite Data(A case study)

  • Chung, Hyo-Sang;Hwang, Byong-Jun;Kim, Young-Haw;Son, Eun-Ha
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 1999년도 Proceedings of International Symposium on Remote Sensing
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    • pp.214-218
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    • 1999
  • The aim of our study is to develop new algorism for sea fog detection by using Geostational Meteorological Satellite-5(GMS-5) and suggest the techniques of its continuous detection. So as to detect daytime sea fog/stratus(00UTC, May 10, 1999), visible accumulated histogram method and surface albedo method are used. The characteristic value during daytime showed A(min) > 20% and DA < 10% when visble accumulated histogram method was applied. And the sea fog region which detected is of similarity in composite image and surface albedo method. In case of nighttime sea fog(18UTC, May 10, 1999), infrared accumulated histogram method and maximum brightness temperature method are used, respectively. Maximum brightness temperature method(T_max method) detected sea fog better than IR accumulated histogram method. In case of T_max method, when infrared value is larger than T_max, fog is detected, where T_max is an unique value, maximum infrared value in each pixel during one month. Then T_max is beneath 700hpa temperature of GDAPS(Global Data Assimilation and Prediction System). Sea fog region which detected by T_max method was similar to the result of National Oceanic and Atmosheric Administration/Advanced Very High Resolution Radiometer (NOAA/AVHRR) DCD(Dual Channel Difference). But inland visibility and relative humidity didn't always agreed well.

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Flow blockage analysis for fuel assembly in a lead-based fast reactor

  • Wang, Chenglong;Wu, Di;Gui, Minyang;Cai, Rong;Zhu, Dahuan;Zhang, Dalin;Tian, Wenxi;Qiu, Suizheng;Su, G.H.
    • Nuclear Engineering and Technology
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    • 제53권10호
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    • pp.3217-3228
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    • 2021
  • Flow blockage of the fuel assembly in the lead-based fast reactor (LFR) may produce critical local spots, which will result in cladding failure and threaten reactor safety. In this study, the flow blockage characteristics were analyzed with the sub-channel analysis method, and the circumferentially-varied method was employed for considering the non-uniform distribution of circumferential temperature. The developed sub-channel analysis code SACOS-PB was validated by a heat transfer experiment in a blocked 19-rod bundle cooled by lead-bismuth eutectic. The deviations between the predicted coolant temperature and experimental values are within ±5%, including small and large flow blockage scenarios. And the temperature distributions of the fuel rod could be better simulated by the circumferentially-varied method for the small blockage scenario. Based on the validated code, the analysis of blockage characteristics was conducted. It could be seen from the temperature and flow distributions that a large blockage accident is more destructive compared with a small one. The sensitivity analysis shows that the closer the blockage location is to the exit, the more dangerous the accident is. Similarly, a larger blockage length will lead to a more serious case. And a higher exit temperature will be generated resulting from a higher peak coolant temperature of the blocked region. This work could provide a reference for the future design and development of the LFR.