• Current Research on Agriculture and Life Sciences
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• v.16
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• pp.31-36
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• 1998

In order to investigate the effect of cytokinins on the cotyledonary variability of somatic embryos in Heracleum moellendorffii Hance., somatic embryos were induced from the immature zygotic embryo on MS medium containing 1.0mg/l 2, 4-D, and then transferred to 2,4-D-free and cytokinin-added medium for somatic embryogenesis after 4weeks culture. Polycotyledonary embryos were formed most abundantly(84.9%) on the 0.2mg/l BA medium as compared with the 0.2mg/l 2ip(42.6%) and kinetin(32.9%) media, and it was proportional to BA concentrations(0.01~0.1mg/l). The rate of polycotyledonary embryo formation increased proportional to the period of BA treatment and also increased more at the globular stage than at the heart, torpedo-shaped and cotyledonary stages.

• Journal of the Korea Organic Resources Recycling Association
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• v.22 no.4
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• pp.87-94
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• 2014

Objective of this study was to investigate characteristics of inorganic components contained in liquid fertilizer produced using bone powder and rice bran by adding dry yeast and molasses. Addition of dry yeast to liquid fertilizer resulted in little change in pH, considerable increase in EC, and it showed high EC value compared to the control which has no additives. Also, it was appeared that the dry yeast-added treatment had higher at $2,936mg{\cdot}L^{-1}$ of $NH_4$-N concentration than the control which had $1,782mg{\cdot}L^{-1}$. In the other hand, addition of molasses resulted in low pH and slightly low EC, as compared to the control. $NH_4$-N concentration in the no added molasses treatment was $2,936mg{\cdot}L^{-1}$ higher than its molasses added treatment which had $2,378mg{\cdot}L^{-1}$. In conclusion, it was shown that addition of dry yeast to liquid fertilizer increased ammonium nitrogen concentration by accelerating nitrogen mineralization, while molasses has an effect of inhibiting nitrogen mineralization and enhancing the characteristics of fermentation. With application of organic liquid fertilizer containing bone powder and rice bran increased the fresh weight of Allium tuberosum.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.8B
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• pp.719-726
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• 2008

In this thesis investigate the security gateway that manage authorization for user access dynamically by recognizing automatically and comparison & distinction between database and User-information while a terminal unit(PC) trying to access to the network of subnet. Also, it present User-interfaced authorization allowance role model, so administrator can assign united access control between network level(L2) and application level(L7) in relation to system construction.

• Korean Journal of Medicinal Crop Science
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• v.9 no.2
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• pp.146-149
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• 2001

This experiment was carried out to find the proper way of shoot induction and elongation of shoots in Rehmannia glutinosa. Shoot induction and multiplication rate from stem explants were significantly improved in the induction media with 1.0mg/L thidiazuron compared to BA and NAA combinations. Shoot elongation was better in the media with thidiazuron 1.0mg/L alone than in the presence of paclobutrazol.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.2
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• pp.156-162
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• 2011

The objective of this study is to evaluate antioxidant activities of black ginseng prepared by nine repeated steaming-drying cycles. Ethanol extracts from each cycle of ginseng showed 33.5~41.0% of yields, 36.2~44.5% of moisture content and $64\sim66^{\circ}Brix$ of soluble solids. As the number of steaming-drying cycles increased, pH decreased, while the absorbance at 420 nm increased remarkably after the 4th cycle. Although the amounts of Rg1 and Rb1 contents quite decreased, the total phenol content of black ginseng (the final cycle of ginseng) was increased to 126%, compared with that of white ginseng. Antioxidant activities, determined by ferric-reducing antioxidant potential (FRAP), 2,2'-azinobis(3 ethybenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, 1,1-diphenyl-2-picrydrazyl (DPPH) and hydroxyl radical scavenging activities, increased remarkably as the number of steaming-drying cycles increased. Especially, FRAP value increased 155.6%. Also, $IC_{50}$ values for DPPH and hydroxyl radical scavenging activities of the final 9th-cycling product, decreased 4.5 folds and 9.7 folds, respectively, compared with those of white ginseng. Based on these results, it was suggested that antioxidant activities of black ginseng improve according to the increasing number of steaming-drying cycles, which was derived from increase of total phenol content.

• The Korean Journal of Food And Nutrition
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• v.17 no.3
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• pp.286-293
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• 2004

Extract of Eleutherococcus senticosus, Lycium chinensis, Angelica acutiloba and Schizandra chinensis were investigated to determine whether its addition may inflict on growth of lactic acid bacteria, freeze dry and cell viability during the storage. In cultivation with herbal extract, all strains tested did not demonstrate a significant reduction in their cell population, particularly Lactobacillus. acidophilus, L. bulgaricus, L. paracasei. But Schizandra chinensis extract inhibited growth of several strains. Immediately after freeze-drying using the skim milk 10%(w/v), sucrose 5%(w/v) with herbal extract 2.5%(v/v), the percentage viability was about 81.7%, 63.8%, 73.2%, 78.1 % in L. acidophilus, L. bulgaricus, L. paracasei, L. casei respectively. The protective effect of herbal extract to cell damage from freeze-drying was weak in comparison with control. During accelerated storage of freeze-dried lactic acid bacteria, those survival rate decrease rapidly, reaching 8 -18% in one month. But addition of Eleutherococcus senticosus extract in freeze dry of L. acidophilus showed a positive activity in storage.

• Journal of Animal Environmental Science
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• v.8 no.3
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• pp.145-152
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• 2002

This experiment was conducted to investigate the efficiency and compatibility of the coagulation and settling processes of leachates from the compost of two swine farms. And results obtained are as follows : 1 In the farm A where $COD_{Cr}$, $COD_{Mn}$ and $BOD_5$ of original leachate were 4,400, 2,950 and 87mg/l, respectively, the rate of coagulation and settling process was more efficient in the leachate treated with the conjugate of Alum and cation polymer than that of Alum and anion polymer. The concentrations of BOD$_{5}$, T-N and T-P of the effluent after treatment with the conjugate of Alum and cation polymer under the optimum condition were 19, 257.5 and 0.4mg/l, respectively which are under the governmental regulation level. 2. In the farm B where $COD_{Cr}$, $COD_{Mn}$ and $BOD_5$ of original leachate were 4,720, 3,040 and 95mg/l, respectively, the conjugate of $FeCl_3$, 1,500mg/l and cation polymer 10mg/l ($FeCl_3$+FO4240) was most effective coagulation and settling agent compared with the others. The concentrations of BOD$_{5}$, T-N and T-P of the effluent after treatment with $FeCl_3$+FO4240 were 15.3, 829.4 and 2.8mg/l, respectively. And the concentration of T-N was higher than the governmental regulation level, presumably because of too high concentration of NH$_4$$^{+}$-N in the leachate.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2007.11a
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• pp.157-158
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• 2007

AISI316L강의 내식성과 표면경도를 동시에 향상시키기 위한방법으로 저온 플라즈마 침탄과 저온 플라즈마 질화를 동일한 로 내에서 연속적으로 실시하였다. 여러 가지 공정인자 중 저온 플라즈마 질화 시 $N_2$가스가 표면에 미치는 영향을 조사 하였다. 모든 시편의 표면에 N에 의해 확장된 오스테나이트 (${\gamma}_N$)가 형성되었으며, 형성된 ${\gamma}_N$로 인하여 표면경도가 약 $3{\sim}4$배 증가하였다. $N_2$가스가 증가할수록 ${\gamma}_N$층의 두께가 증가 하였다.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.238-245
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• 1999

Antioxidative effects of the water or ethanol extracts from Rhus verniciflua Stokes (RVS) were measured by protection against hydroxyl radicals in mouse brain tissue culture. In the water extracts from RVS, cell viabilities were estimated 60.0, 66.0, 72.0, 84.0 and 90.0% at addition of 1, 2, 4, 7 and $10{\mu}L$, respectively, compared with GO (20 mU/mL) alone. The cell viability in the ethanol extracts was similarly with water extracts. In the antitumor effects, the results showed that percentages of the HeLa cell death were approximately 24% for 12 hrs, 57% for 48 hrs at addition of 10%/well ethanol extracts respectively. To know inhibition of tumor growth, in vivo, mice (BALB/c) were inoculated with 0.25 mL CT-26 $(1{\times}10^6\;cells/mL)$ subcutaneously. After the generation of tumor, the results of RVS extracts (ethanol, water) injection showed generally that the tumor size in BALB/c was reduced. For physicochemical characterization of the RVS extracts, purified substances of water or ethanol extracts were analized with SDS-PAGE and ICP spectrometer. In electrophoresis, gel showed 2 bands (210, 230 KDa). The results of ICP verified that RVS extracts contain $Cu^{2+}$ in both samples. Conclusively, this substance might be a laccase which has a biological effective function, as a natural bioactive substance.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.46-46
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• 2015

강우가 지역별 계절별로 편중되어 있는 우리나라는 수자원의 안정적인 확보와 이용을 위해 다양한 형태의 댐을 건설하여 운영하고 있다. 그러나 대부분의 댐건설을 통해 형성된 저수지들은 탁수 장기화 및 녹조 발생 등의 환경, 생태적인 문제를 겪고 있으며, 그에 따른 사회적 우려로 인해 신규댐 건설을 통한 수자원확보는 더 이상 어려운 실정이다. 이러한 문제에 대응하기 위한 대안으로 기존 댐 저수지들(안동호-임하호)의 구조적 연계운영방안이 진행되고 있다. 본 연구의 목적은 2차원 CE-QUAL-W2모형을 활용하여 안동호와 임하호의 구조적 연결에 따른 탁수의 이동과 각 저수지 내에서의 유동 변화를 해석하는데 있다. 저수지 연계 시나리오는 EL. 138 m 위치에 길이 2 km, 직경 5.5 m 의 콘크리트관(마찰계수 0.05)이 안동호 좌안인 임동면 마리와 임하호 우안 망천리를 연결하는 것으로 가정하였다. 모델의 보정은 실측자료가 풍부한 2006년도 수문사상을 대상으로, 개별 저수지에 대해 수행하였고, 탁수 유동 시나리오 해석은 임하호에 심각한 탁수장기화 문제가 발생했던 2002년을 대상으로 댐 연계 탁수모의를 수행하였다. 안동호와 임하호의 댐 앞에서 모의값과 실측값을 오차를 분석한 결과 탁수예측오차는 AME 0.5~24 mg/L, RMSE 0.7~30.2mg/L의 범위로 비교적 실측값을 잘 반영한 것으로 나타났다. 임하댐의 경우 탁수층의 위치와 두께, 그리고 최고 탁도값을 적절히 재현 하였지만, 안동댐은 최고 탁도값 예측에서 다소 오차가 발생하는 것으로 나타났다. 안동호와 임하호 단독 운영시와 연계 운영시의 탁수변화 파악을 위해 초기 홍수사상이 발생한 8월 이후부터 저수지내의 TSS농도 분포를 비교하였다. 안동호의 경우 댐앞지점의 탁수분포는 수온성층구조에 영향을 받아, 단독 운영시(EL. 130 m)보다 연계운영시(EL. 140 m)에 탁수의 중심이 높은 위치에 형성되었다. 단독 운영시 10월 이후에 전도현상으로 인해 침강되지 않은 잔류 탁수층이 저수지 하부로 확산되었지만, 연계 운영시에는 재부상 되어 상층으로 확산되는 것으로 모의되었다. 또한 연계운영시 유량이동으로 인해 안동호의 탁수 댐앞 도달시간이 짧아지는 것으로 나타났다. 반면 임하호는 연계 운영시 안동댐으로 유출이 생기면서 중층에서 탁수량이 저감되는 것으로 모의되었다. 저수지 내 탁수량 분석을 위해 SS 15 mg/L 이상의 잔류 탁수량을 분석한 결과, 연계운영시 안동호의 평균 잔류탁수량 비율은 11.8% 증가, 임하호의 경우 11.7% 감소하였다. 또한, 탁수의 댐하류 방류일수도 SS 15 mg/L 기준 임하호 9일 저감, 안동호는 70일 증가하여 임하호의 탁수가 안동호의 탁수 장기화에 영향을 주는 것으로 나타났다.