• Title/Summary/Keyword: korean native chicken

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Atomic Force Microscopy of Asymmetric Membranes from Turtle Erythrocytes

  • Tian, Yongmei;Cai, Mingjun;Xu, Haijiao;Ding, Bohua;Hao, Xian;Jiang, Junguang;Sun, Yingchun;Wang, Hongda
    • Molecules and Cells
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    • v.37 no.8
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    • pp.592-597
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    • 2014
  • The cell membrane provides critical cellular functions that rely on its elaborate structure and organization. The structure of turtle membranes is an important part of an ongoing study of erythrocyte membranes. Using a combination of atomic force microscopy and single-molecule force spectroscopy, we characterized the turtle erythrocyte membrane structure with molecular resolution in a quasi-native state. High-resolution images both leaflets of turtle erythrocyte membranes revealed a smooth outer membrane leaflet and a protein covered inner membrane leaflet. This asymmetry was verified by single-molecule force spectroscopy, which detects numerous exposed amino groups of membrane proteins in the inner membrane leaflet but much fewer in the outer leaflet. The asymmetric membrane structure of turtle erythrocytes is consistent with the semi-mosaic model of human, chicken and fish erythrocyte membrane structure, making the semi-mosaic model more widely applicable. From the perspective of biological evolution, this result may support the universality of the semi-mosaic model.

Gene Expression Profiling by RNA Sequencing in Mature/Immature Oocytes of Chicken (닭의 성숙/미성숙란에서 RNA Sequencing을 이용한 유전자 발현 양상 고찰)

  • Kang, Kyung-Soo;Jang, Hyun-Jun;Park, Mi Na;Choi, Jung-Woo;Chung, Won-Hyong;Heo, Kang-Nyeong;Choe, Chang-Yong;Kim, Young-Joo;Lee, Si-Woo;Cho, Eun-Seok;Kim, Namshin;Kim, Tae-Hun;Han, Jae-Yong;Lee, Kyung-Tai
    • Korean Journal of Poultry Science
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    • v.41 no.4
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    • pp.287-296
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    • 2014
  • Chicken eggs undergo various physiological changes during egg maturation. To study genes associated with the egg maturation in pre-ovulation (immature) and post-ovulation (mature), we compared gene expression patterns between in the immature egg and mature egg using RNA sequencing data. Mature and immature eggs were obtained from a Heuksaek Jaerae-jong of Korean native chicken. Total RNAs obtained from the eggs were sequenced by Illumina HiSeq 2000 platform, and the generated sequence reads were mapped to Galgal4 reference sequence assembly using Tuxedo Protocol. From the comparison of the RNA sequencing data, 315 genes were differentially expressed between mature and immature eggs, and 46 genes were only detected in immature egg. Further gene ontology (GO) analysis was performed for the differentially expressed genes using DAVID, showing that 29 and 28 GO terms were independently clustered from mature and immature, respectively. From those clustered GO terms, genes related to germ cell development, sex differentiation and defense response to bacterium were mainly expressed in the immature egg, while genes related to regulation of apoptosis, steroid metabolic process and lipid homeostasis were mainly detected in the mature egg. Our results could contribute to understand egg maturation before and after ovulation, and develop genetic markers for improving egg quality and productivity.

The Effect of Early Chick Weight on Market-Weight in Korean Native Chickens (토종닭의 초기성장체중이 출하체중에 미치는 영향)

  • Kim, Ki Gon;Choi, Eun Sik;Kwon, Jae Hyun;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.44 no.4
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    • pp.259-265
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    • 2017
  • This study was to investigate the effect of chick weight in the early growth period on market-weight of Korean native chickens (KNCs). We measured the body weights of 1,087 chickens (male 479, female 608) of 13 KNC strains at 1-84 days of age at two week intervals. The growth performance of the 13 KNC strains was investigated. Correlation coefficients among the weights of chickens in each growth period and regression of market-weight on early chick weights were analyzed. The results showed that the average body weight of 70-day-old KNCs was 1,962g: 2,154g and 1,819.7g for males and females, respectively. The equation for regression of body weight on age was estimated as $\hat{Y}=0.1347X^2+18.738X-40.134$ ($R^2=0.9418$). Using this regression equation, the duration required to attain a KNC market-weight of 2 kg was estimated as 71.8 days. All the correlation coefficients between early chick weight and market-weight were significantly positive. Although the correlation coefficients among the chick weights in each growth period decreased with increase in age interval, early growth weight had a significant effect on late growth performance. The correlation coefficient between market-weight at 70-days and chick weight at 1-day was estimated to be a low as 0.10-0.13. In the estimations of market-weight correlation coefficients, correlation coefficient and coefficient of determination were high in the female and male chicks after 28-days and 42-days of age, respectively. The results of the analysis of correlation and regression between early chick weight and market-weight of KNCs showed that market-weight could be predicted based on the weights of 28-day-old females and 42-day-old males.

Crossbreeding Combination Test for the Production of New Synthetic Korean Native Commercial Chickens (신품종 토종닭 생산을 위한 교배조합 검정시험)

  • Sohn, Sea Hwan;Choi, Eun Sik;Cho, Eun Jung;Kim, Bo Gyeong;Shin, Ka Bin;Lee, Seul Gy;Oh, Ki Suk
    • Korean Journal of Poultry Science
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    • v.48 no.3
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    • pp.101-110
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    • 2021
  • In this study, we performed a cross-combination test using offspring from two paternal and five maternal parent stock lines to produce a new synthetic breed of Korean native commercial chickens. The survival rate, body weight, feed intake, and feed efficiency were measured for 10 crosses, with 837 Hanhyup-3 chickens used as a control. Most chickens had brown-based feathers, and there was little difference in color phenotypes among combinations. The average survival rate of the combinations was 94.9%, which was significantly different from that of Hanhyup-3 at 75.3% (P<0.01). There was significant differences in body weight among the combinations at all weeks of age (P<0.01). At 10 weeks of age, the FHHY combination was the heaviest with 2,300 g, while the weight of Hanhyup-3 was 1,922 g. In addition, the weight difference between the male and female chickens was 458 g. As a result of regression analysis, the duration needed to reach 1.9 kg was about 61 days for the FHFY, FHHY, and HFFY combinations, and 70 days for Hanhyup-3, exhibiting a 9-day difference. The average feed conversion ratio of the combinations until 10 weeks was 2.42, which was significantly different from that of Hanhyup-3 at 2.97 (P<0.01). In conclusion, FHHY, FHFY, and HFFY were determined to be the best new breed combinations based on production capacity. When these combinations are used as new commercial breeds, they are expected to increase the average weight by 18% and improve feed efficiency by 19%, as compared to the existing Hanhyup-3 breed.

Association of SNPs in the HNF4α Gene with Growth Performance of Korean Native Chickens (한국 재래계의 HNF4α 유전자 내 SNP와 성장과의 연관성 분석)

  • Yang, Song-Yi;Choi, So-Young;Hong, Min-Wook;Kim, Hun;Kwak, Kyeongrok;Lee, Hyojeong;Jeong, Dong Kee;Sohn, Sea Hwan;Hong, Yeong Ho;Lee, Sung-Jin
    • Korean Journal of Poultry Science
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    • v.45 no.4
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    • pp.253-260
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    • 2018
  • The hepatocyte nuclear factor 4 alpha ($HNF4{\alpha}$) gene is related to lipid transport, including abdominal fat and growth, in chickens. Interestingly, the A543G SNP within the $HNF4{\alpha}$ gene has previously been reported to be associated with body weight in both broilers and Korean native chickens (KNCs). However, its exact position within the HNF4 is not yet reported. This study aimed to identify the position of the A543G SNP and to identify additional SNPs that can be used as genetic markers in KNCs. A total of 128 KNCs were used for the sequencing and analysis of these genetic associations. As a result, A543G SNP was located in intron 4 of the $HNF4{\alpha}$ gene; it is reported as rs731246957 in the NCBI database. Fourteen SNPs were detected in the sequenced portion of the $HNF4{\alpha}$ gene; three of these, rs731246957, rs736159604 and new SNP, intron 6 (249), were significantly related with growth in the chickens. In this study, the TT genotype of rs731246957, previously reported as A543G SNP, the GG genotype of rs736159604 and GT of new SNP have are highly associated with body weight from birth to 40 weeks of age in KNCs (P<0.01). These results suggest that rs736159604, rs731246957 and intron 6 (249) SNPs within the $HNF4{\alpha}$ gene could function as growth-related markers in the selective breeding of KNCs.

Effects of Stock Density and Nutrient Levels on Growth Performance, Serum Profile, Immune Status and Meat Quality in Korean Native Chickens (토종 실용계의 사육밀도 및 사료 내 에너지 수준에 따른 생산성, 혈액, 면역 및 계육 품질에 미치는 영향)

  • Kim, KwangYeol;Jeon, Jin-Joo;Kim, Hyunsoo;Son, Jiseon;Kim, Hee-Jin;You, Are-Sun;Hong, Eui-Chul;Kang, Boseok;Kang, Hwan Ku
    • Korean Journal of Poultry Science
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    • v.48 no.2
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    • pp.91-100
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    • 2021
  • The study determined the effects of stock density (SD) and energy level (EL) on growth performance, serum biochemistry, and meat quality in Korean native chickens. A total of 240 chickens were randomly assigned to one of the four treatments including two stock density (low, 14, and high, 16 chickens per m2) and two energy level (low, 2,950, 3,000, 3050 ME kcal/kg, and high 3,150, 3,200, 3,250 ME kcal/kg, for starter, grower, and finisher, respectively). During the whole period of the experiment, the chickens were fed ad libitum. The greater final body weight and weight gain were achieved in chickens fed high energy diet, whereas the final body weight and weight gain were significantly reduced in high-density treatment compared with the low density. Chickens in low-density groups had a higher feed intake compared with high-density treatment, however, the energy level did not affect the feed intake. An improved overall feed conversion ratio was detected in the high energy treatment. There was a significant interaction between stock density and energy level on cholesterol concentration. The concentration of aspartate transaminase in serum was increased by higher stock density. There was a significant treatment interaction on IgM levels. Moreover, the carcass rate was significantly increased in the high energy level treatment. Based on the findings, we suggest that rearing chickens in low density with high dietary energy levels could be beneficial by improving the growth performance.

Cryopreservation of Primordial Germ Cells(PGCs) from Korean Native Chicken(Ogye) Embryos using Commercial Cryoprotectants (상업용 동결보호제를 이용한 한국재래닭(오계) 원시생식세포의 동결 보존)

  • Kim, Hyun;Kim, Dong Hun;Han, Jae Yong;Do, Yoon Jung;Kim, Jae Hwan;Kim, Young Sin;Seong, Hwan Hoo;Ko, Yeoung Gyu;Kim, Sung Woo
    • Korean Journal of Poultry Science
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    • v.40 no.3
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    • pp.163-169
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    • 2013
  • Cryopreservation of poultry semen has been reported, but preservation of female genetic material has not been possible because of the unique anatomical and physiological characteristics of the avian egg. Thus an alternative strategy for conservation of oviparous species of animals must be developed. Recent technological developments for producing germline chimeras by the transfer of primordial germ cells (PGCs) into recipient embryos has enabled the conservation and retrieval of chicken genetic resources in their complete form. In the present study, fertilized eggs were incubated for about 5.5 days to obtain embryos at stage 28. The whole embryo was collected from the germinal gonad using a fine glass micro pipette under a microscope. The PGCs were then purified using MACS method. Two commercially available cryoprotectants (A and B) were used to preserve the PGCs, and EG were used as a control. The average recovery rate of PGCs after thawing was 35.5% and 60.5% with the A and B treatments, respectively. There was no significant difference between B treatments and control, which showed an average recovery rate of 52.8%. However, the recovery rate obtained using A cryoprotectant (35.5%) was significantly lower than using treatment control and B. The average viability of the PGCs after thawing were 77.9% and 77.4% for cryoprotectants A and B, respectively, and the control were was 81.6%. There was no statistically significant difference between the two treatments and control. It was concluded that all of the available cryoprotectants examined in this study could be used for preservation of PGCs from embryos. Further experiments to produce germline chimera from PGCs preserved using this techniques are strongly recommended.

A Study on the Menu Development for the Elderly during Busy Farming Season in Gyeongbuk Andong (경북 안동시 농번기 농촌노인 식단개발 연구)

  • Kim, Hae-Young;Park, Chan-Eun;Lee, Hae-Jin;Park, Young-Hee;Lee, Jin-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.10
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    • pp.1381-1391
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    • 2009
  • Dietary life style of the elderly living in rural areas of Gyeongbuk Andong during the busy farming season was surveyed and a monthly meal plan for the elderly was developed using eco-friendly native crops and considering the characteristics of a busy farming season and their preferences. A week meal plan was selected and applied to those fields. The meal plan was developed for an elder's house in Gyeongbuk Andong during the busy farming season. Especially, to increase the intake of calcium and dietary fiber, bamboo sprouts, pepper leaves, dried slices of whitebait, and fried anchovies were used. Considering the busy farming season, the ginseng chicken soup and the soybean noodle soup were served to provide high quality of protein and to improve health condition. A cold soup of cucumber and brown seaweed was used to supplement liquid, vitamins, and minerals. The results are as follows: the highest score of the preference on a staple was 8.77 for the boiled barley of menu 5; the favorite soup was the ginseng chicken soup (8.73) in menu 4; for side dishes, those in menu 5 were most popular (8.69); the favorite meal was the ginseng chicken soup of menu 4 (8.69). Regarding the amount of leftover foods, the lightest was 30.14 g in Gyeongbuk Andong menu 3 which significantly indicates high preference (p<0.05). The current study was a planned meal service menu using local crops and seasonal foods, which was actually applied to the field resulting in high preference and satisfaction levels.

Growth and Development of the Academic Societies and Animal Production in Korea, China and Asia over the Last 50 Years

  • Han, In K.;Ha, Jong K.;Lee, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.6
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    • pp.906-914
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    • 2009
  • The Korean Society of Animal Science (KSAS) was officially born on October 8, 1956 under the leadership of Professor Sang W. Yun of Seoul National University, Seoul, Korea a few years after the end of the Korean War. At that time, there were 0.9 million Korean native cattle, 1.3 million pigs and 8.9 million chickens in Korea. Per capita income for Korea (US$ 66) or China (US$ 59) was about 10% of Asian's average income (US$ 513) in 1956. Korea produced less than 0.2 million M/T of formula feed and consumed 6.1 kg/person/year of animal products. One could say that Korea was an example of an under-developed country in the world. Although the first issue of the Proceedings of the KSAS was published on October 28, 1958, regular quarterly journals of the KSAS were not published until March 1, 1969. Major activities other than publishing its journal were: holding an annual meeting and/or scientific forum at national or international level. The Asian-Australasian Association of Animal Production Societies (AAAP) was founded on September 1, 1980 at Kuala Lumpur, Malaysia with founding members from Australia, Indonesia, Japan, Korea, Malaysia, NZ, Philippines and Thailand. Thirteen AAAP Animal Science Congresses have been held in its 28 year history. Hosting countries were Malaysia (1980), Philippines (1982), Korea (1985), NZ (1987), Taiwan (1990), Thailand (1992), Indonesia (1994), Japan (1996), Australia (2000), India (2002), Malaysia (2004), Korea (2006) and Vietnam(2008). In 1988, significant progress of the AAAP was made by creating an official English journal of the AAAP entitled "Asian-Australasian Journal of Animal Sciences (AJAS)" under the initiative of the KSAS. This journal is now published monthly and distributed to more than 50 countries in the AAAP region and the world. It should be mentioned that the KSAS was able to successfully host the $3^{rd}$ AAAP Animal Science Congress in 1985 and the 12th in 2006, as well as the $8^{th}$ WCAP in 1998. During the last 50 years of KSAS history, per capita income of Korea increased to US$ 17,690 (268 fold), formula feed production increased to 15 million M/T (97 fold) and consumption of animal products increased to 105 kg/person/year (17 fold). Cattle, pig and chicken numbers also increased to 2.5 million (2.8 fold), 9 million (7.4 fold) and 119 million (13 fold). This trend was also found for China and Asia, even if the rate of growth was slightly lower than that of Korea. It is expected that a similar rate of growth in economics, animal numbers, formula feed production and animal protein intake will likely be achieved by other Asian countries in the $21^{st}$ century with somewhat lower rate of development than that of Korea.

Effects of High Stocking Density on the Expression of Metabolic Related Genes in Two Strains of Chickens (닭의 고밀도사양 스트레스가 품종 간 체내대사 유전자 발현에 미치는 영향)

  • Sohn, Sea Hwan;Jang, In Surk;An, Young Sook;Moon, Yang Soo
    • Korean Journal of Poultry Science
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    • v.42 no.1
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    • pp.51-59
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    • 2015
  • Chickens are exposed to the external and internal stressors such as low and high temperature, high stocking density, feed restriction and disease. There have been a few studies on gene expressions through the investigation of chickens under direct exposure to the stress of high stocking density. The objective of the present study was to determine the expressions of genes associated with stress, endoplasmic reticulum (ER)-stress, lipid and glucose metabolism in two strains of chickens, Korean Native Chicken (KNC) and White Leghorn (WL), raised in high stocking density. A total of 164 chickens aged 40 weeks were randomly allotted to a $540cm^2/bird$ stocking density (control), whereas the chickens in a high density group were assigned in a $311cm^2/bird$ stocking density with feeding ad libitum for 10 weeks. Total RNA was extracted from the live for qRT-PCR. The expression levels of hsp70 and $hsp90{\alpha}$ were higher in WL subjected to stress with high stocking density compared with those genes in control (P<0.05), while the expressions of genes were not affected in KNC. ER stress marker gene XBP1 was also highly expressed in WL with stress (P<0.05), but the stress of high stocking density did not influence to ER stress marker genes in KNC. Lipid metabolism associated genes including FABP4, FATP1 and ACSL1 were highly expressed in WL compared with KNC when subjected to high stocking density stress (P<0.05). The expression of glucose transport gene GLUT2 and GLUT8 were increased in chickens exposured to the stress of high stocking density (P<0.05). The data indicate that WL is more sensitive to the stress of high stocking density compared with KNC and the stress may influence the modulation of lipid and glucose metabolism in the liver of chickens.