한국가금학회지 (Korean Journal of Poultry Science)

  • 제41권4호
  • /
  • Pages.287-296
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  • 2014
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
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닭의 성숙/미성숙란에서 RNA Sequencing을 이용한 유전자 발현 양상 고찰

Gene Expression Profiling by RNA Sequencing in Mature/Immature Oocytes of Chicken

  • 강경수 (농촌진흥청 국립축산과학원) ;
  • 장현준 (농촌진흥청 국립축산과학원) ;
  • 박미나 (농촌진흥청 국립축산과학원) ;
  • 최정우 (농촌진흥청 국립축산과학원) ;
  • 정원형 (한국생명공학연구원 국가생명연구자원정보센터) ;
  • 허강녕 (농촌진흥청 국립축산과학원) ;
  • 최창용 (농촌진흥청 국립축산과학원) ;
  • 김영주 (농촌진흥청 국립축산과학원) ;
  • 이시우 (농촌진흥청 국립축산과학원) ;
  • 조은석 (농촌진흥청 국립축산과학원) ;
  • 김남신 (한국생명공학연구원 국가생명연구자원정보센터) ;
  • 김태헌 (농촌진흥청 국립축산과학원) ;
  • 한재용 (서울대학교 농생명공학부) ;
  • 이경태 (농촌진흥청 국립축산과학원)
  • 투고 : 2014.10.08
  • 심사 : 2014.11.11
  • 발행 : 2014.12.31
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초록

조류의 난포 성장은 호르몬의 작용에 따라 크기가 달라져 각각의 단계를 이루며 성장하게 된다. 난의 성숙에 관련된 유전자는 난 단백질 생산과 산란률에 밀접한 관련이 있으며, 이를 유전자 발현 측면에서 심도 있는 고찰이 필요가 있다. 본 연구는 NGS를 이용한 RNA-seq 데이터를 이용하여 유전자의 발현량과 유전자 상호 구조에 대한 분석을 실시하여 난의 발달 과정에 필요한 유전자군을 조사하였다. 본 실험에 사용된 개체는 한국 재래계 흑색계통이 사용되었고, 비교조직은 미성숙란과 성숙란의 RNA를 추출하여 유전자의 발현 양상을 살펴봄으로 난의 성숙에 필요한 유전자의 발현 양상을 보고자 하였다. 실험을 위해 Total RNA를 추출하였고, HiSeq 2000 platform을 사용하여 염기서열을 분석하고, Tuxedo Protocol과 DAVID 프로그램을 통해 유전자의 기능과 상호간의 연관관계를 예측하였다. 탐색된 유전자군은 미성숙란과 성숙란 간에 많은 차이를 보이고 있는 유전자군을 탐색한 결과, 315개의 발현이 다르게 나타나는 것으로 보이고 있으며, GO 분석을 통하여 기능면에서 미성숙란과 성숙란에서 확연히 구분되는 유전자 발현 양상을 확인할 수 있었다. 이들 결과를 통하여 향후 난성숙 과정을 이해하고, 계란 품질 향상을 위한 마커 개발을 기여할 수 있을 것으로 사료된다.

Chicken eggs undergo various physiological changes during egg maturation. To study genes associated with the egg maturation in pre-ovulation (immature) and post-ovulation (mature), we compared gene expression patterns between in the immature egg and mature egg using RNA sequencing data. Mature and immature eggs were obtained from a Heuksaek Jaerae-jong of Korean native chicken. Total RNAs obtained from the eggs were sequenced by Illumina HiSeq 2000 platform, and the generated sequence reads were mapped to Galgal4 reference sequence assembly using Tuxedo Protocol. From the comparison of the RNA sequencing data, 315 genes were differentially expressed between mature and immature eggs, and 46 genes were only detected in immature egg. Further gene ontology (GO) analysis was performed for the differentially expressed genes using DAVID, showing that 29 and 28 GO terms were independently clustered from mature and immature, respectively. From those clustered GO terms, genes related to germ cell development, sex differentiation and defense response to bacterium were mainly expressed in the immature egg, while genes related to regulation of apoptosis, steroid metabolic process and lipid homeostasis were mainly detected in the mature egg. Our results could contribute to understand egg maturation before and after ovulation, and develop genetic markers for improving egg quality and productivity.

키워드

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