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http://dx.doi.org/10.5536/KJPS.2014.41.4.287

Gene Expression Profiling by RNA Sequencing in Mature/Immature Oocytes of Chicken  

Kang, Kyung-Soo (National Institute of Animal Science, RDA)
Jang, Hyun-Jun (National Institute of Animal Science, RDA)
Park, Mi Na (National Institute of Animal Science, RDA)
Choi, Jung-Woo (National Institute of Animal Science, RDA)
Chung, Won-Hyong (Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology)
Heo, Kang-Nyeong (National Institute of Animal Science, RDA)
Choe, Chang-Yong (National Institute of Animal Science, RDA)
Kim, Young-Joo (National Institute of Animal Science, RDA)
Lee, Si-Woo (National Institute of Animal Science, RDA)
Cho, Eun-Seok (National Institute of Animal Science, RDA)
Kim, Namshin (Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Tae-Hun (National Institute of Animal Science, RDA)
Han, Jae-Yong (Department of Agricultural Biotechnology, Seoul National University)
Lee, Kyung-Tai (National Institute of Animal Science, RDA)
Publication Information
Korean Journal of Poultry Science / v.41, no.4, 2014 , pp. 287-296 More about this Journal
Abstract
Chicken eggs undergo various physiological changes during egg maturation. To study genes associated with the egg maturation in pre-ovulation (immature) and post-ovulation (mature), we compared gene expression patterns between in the immature egg and mature egg using RNA sequencing data. Mature and immature eggs were obtained from a Heuksaek Jaerae-jong of Korean native chicken. Total RNAs obtained from the eggs were sequenced by Illumina HiSeq 2000 platform, and the generated sequence reads were mapped to Galgal4 reference sequence assembly using Tuxedo Protocol. From the comparison of the RNA sequencing data, 315 genes were differentially expressed between mature and immature eggs, and 46 genes were only detected in immature egg. Further gene ontology (GO) analysis was performed for the differentially expressed genes using DAVID, showing that 29 and 28 GO terms were independently clustered from mature and immature, respectively. From those clustered GO terms, genes related to germ cell development, sex differentiation and defense response to bacterium were mainly expressed in the immature egg, while genes related to regulation of apoptosis, steroid metabolic process and lipid homeostasis were mainly detected in the mature egg. Our results could contribute to understand egg maturation before and after ovulation, and develop genetic markers for improving egg quality and productivity.
Keywords
egg maturation; RNA sequencing; gene ontology; differential gene expression;
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