• Title/Summary/Keyword: kit evaluation

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A New ELISA Kit Based on Antigenic Epitopes for Diagnosing Brucella abortus

  • Khodabakhsh, Tannaz;Arabi, Azin;Pakzad, Parviz;Gheflat, ShivaSadat;Bahreinipour, Akram;Bandehpour, Mojgan
    • Microbiology and Biotechnology Letters
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    • v.47 no.1
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    • pp.158-163
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    • 2019
  • Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity.

Development of Balloon-based Autonomous Airborne Robot-kit (풍선기반 자율형 공중로봇키트 개발)

  • Kim, Hyun-Sik
    • The Journal of the Korea institute of electronic communication sciences
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    • v.8 no.8
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    • pp.1213-1218
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    • 2013
  • Recently, although the need of marine robots such as the underwater robot, surface robot and airborne robot being raised in extreme areas, the basis is very deficient. Fortunately, as the need of the robot education is increasing, it is desirable to establish the R&D basis of marine robots and to train future talents through the development and diffusion of marine robot kits. However, in conventional case, there is no marine robot-kit based on the balloon, which has the abilities of the airborne locomotion and obstacle avoidance. To solve this problem, a balloon-based autonomous airborne robot-kit that has the ability of the obstacle avoidance with an infrared sensor, is developed. The test and evaluation results show the possibility of the real applications and the necessity of additional work.

Evaluation of DNA Extraction Methods from Low Copy Number (LCN) DNA Samples for Forensic DNA Typing

  • Eom, Yong-Bin
    • Biomedical Science Letters
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    • v.15 no.3
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    • pp.229-232
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    • 2009
  • DNA isolation for PCR-based short tandem repeat (STR) analysis is essential to recover high yields of amplifiable DNA from low copy number (LCN) DNA samples. There are different methods developed for DNA extraction from the small bloodstain and gloves, commonly found at crime scenes. In order to obtain STR profiles from LCN DNA samples, DNA extraction protocols, namely the automated $iPrep^{TM}$ $ChargeSwitch^{(R)}$ method, the automated $QIAcube^{TM}$ method, the automated $Maxwell^{(R)}$ 16 DNA $IQ^{TM}$ Resin method, and the manual $QIAamp^{(R)}$ DNA Micro Kit method, were evaluated. Extracted DNA was quantified by the $Quantifiler^{TM}$ Human DNA Quantification Kit and DNA profiled by $AmpFISTR^{(R)}$ $Identifiler^{(R)}$ Kit. Results were compared based on the amount of DNA obtained and the completeness of the STR profiles produced. The automated $iPrep^{TM}$ $ChargeSwitch^{(R)}$ and $QIAcube^{TM}$ methoas produced reproducible DNA of sufficient quantity and quality trom the dried blood spot. This two automated methods showed a quantity and quality comparable to those of the forensic manual standard protocols normally used in our laboratory. In our hands, the automated DNA extraction method is another obvious choice when the forensic case sample available is bloodstain. The findings of this study indicate that the manual simple modified $QIAamp^{(R)}$ DNA Micro Kit method is best method to recover high yields of amplifiable DNA from the numerous potential sources of LCN DNA samples.

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Development of RAW Data Storage Equipment for Operation Algorithm research of the Millimeter Wave Tracking Radar (밀리미터파 추적레이더 운용 알고리듬 연구를 위한 RAW 데이터 저장 장비 개발)

  • Choi, Jinkyu;Na, Kyoung-Il;Shin, Youngcheol;Hong, Soonil;Kim, Younjin;Kim, Hongrak;Joo, Jihan;Kim, Sosu
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.22 no.3
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    • pp.57-62
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    • 2022
  • Recently, the tracking radar continues research to develop a new operation algorithm that can acquire and track a target in various environments. In general, modeling similar to the real target and environment is used to develop a new operation algorithm, but there is a limit to modeling the real environment. In this paper, a RAW data storage device was developed to efficiently develop a new operation algorithm required for the tracking radar using millimeter wave to acquire and track the target. The RAW data storage equipment is designed so that the signal processing device of the tracking radar using millimeter wave can save the RAW data output from 8 channels to OOOMSPS. RAW data storage equipment consists of data acquisition equipment and data storage equipment. The data acquisition equipment was implemented using a commercial Xilinx KCU 105 Evaluation KIT capable of high-speed data communication interface, and the data storage equipment was implemented by applying a computer compatible with the commercial Xilinx KCU 105 Evaluation KIT. In this paper, the performance of the implemented RAW data storage equipment was verified through repeated interlocking tests with the signal processing device of the millimeter wave tracking radar.

A Research on Development of Applique Armor Kit for Design of Manufacturing Technology based on the Reverse Engineering (역설계 기반 제조기술 설계를 통한 방탄용 부가장갑킷트 개발에 관한 연구)

  • Ko, Dong Hyeon;Moon, Tae Sang;Kim, Young Ki
    • Journal of Korean Society for Quality Management
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    • v.49 no.3
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    • pp.233-243
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    • 2021
  • Purpose: The study focused on localization of the enhanced applique armor kit (EAAK), which are applied to Korea Assault Amphibious Vehicle (KAAV). Methods: For this propose, we developed the manufacturing technology by analyzing the original products. Considering the conditions used in the military, we made the test evaluation criteria. Results: In results, The EAAK developed through this research performs equivalent performance compared to Rafael, Co. Thus, It is possible not only stably supply a parts of EAAK for maintenance, but also to reduce costs due to import substitution effect. Conclusion: It is expected that the manufacturing technology and test evaluation criteria accumulated through this study can be used in the localization of similar parts.

Evaluation of the Malaria Rapid Diagnostic Kit in Ganghwa County (강화군에서 시행한 말라리아 신속진단킷트의 유용성 평가)

  • Kweon, Su-Jung;Kweon, Oh-Jun;Youn, Sung-Tae;Yim, Jun;Im, Jeong-Soo
    • Journal of agricultural medicine and community health
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    • v.31 no.2
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    • pp.157-164
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    • 2006
  • Objectives: Early diagnosis and treatment is the most important strategy to control malaria effectively. Microscopic examination of blood films is a traditional and standard method for diagnosing malaria, which takes a long time and needs expertise, Therefore, the alternative method, rapid diagnostic kit has been used for quick diagnosis in some counties, a highly infectious region by P. vivax. The purpose of this study was to evaluate the utility of malaria rapid diagnostic kit in Ganghwa county. Methods: The utility was evaluated by mean diagnosis time and sensitivity and specificity. For monitoring mean diagnosis time, 942 cases which were diagnosed for P. vivax were collected between 1998 and 2005, And for calculating sensitivity and specificity, 434 whole bloods in EDTA which were presented for P. vivax by microscopy and rapid diagnostic kit were collected between 2004 and 2005. Results: After malaria rapid diagnostic kit was used in 2003, mean diagnosis time has decreased to 3.36-3.14 day. The sensitivity and specificity of the rapid diagnostic kit was 98.2% and 98,5% and comparable to that of microscopic examination. Conclusions: The malaria rapid diagnostic kit is useful tool in a highly infectious region like Ganghwa county.

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Usability Evaluation and Development of Design Prototyping for MP3 Smart Clothing Product (MP3 스마트웨어 제품 상용화를 위한 디자인 프로토타입 개발 및 사용성 평가 연구)

  • Chea, Heang-Suk;Hong, Ji-Young;Kim, Jun-Hee;Kim, Jin-Hyung;Han, Kwang-Hee;Lee, Joo-Hyeon
    • Science of Emotion and Sensibility
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    • v.10 no.3
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    • pp.331-342
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    • 2007
  • This study focused on developing MP3 smart clothing products and usability evaluation. MP3 smart clothing which was developed in this study contains e-textile keypad and a kit. The kit consists of MP3 Player, remote controller module and ear phone, and are easy to assemble and dismantle. And usability evaluation about MP3 smart Clothing which was developed in this study contains e-textile keypad and a kit. For making the MP3 Smart clothing, e-textile signal line and keypad was developed and a metal connection ring also was manufactured to adhere closely e-textile signal line to keypad. The last products are two kinds of MP3 smart clothing, jacket style of MP3-YSJ(Yonsei Smart Jacket) and safari style of MP3-YSS(Yonsei Smart Safari). Then usability evaluation conducted two times about MP3 smart clothing developed. Usability evaluation is classified by a module evaluation and an item evaluation. The module evaluation measured external appearance, material, a music controller, an ear phone(or a mini speaker) and connector. The item evaluation measured social acceptance, feeling of wearing, utility, easiness of maintenance and safety. The module evaluation described totally positive results in the first usability evaluation about MP3-YSJ 1.0 and MP3-YSS 1.0. The item evaluation shows a lower score in the social acceptance, especially the easiness of connector maintenance and the social acceptance of music-controller in MP3-YSJ 1.0 and MP3-YSS 1.0. The second usability evaluation conducted with improved products by the first usability test results. Results from second item evaluation indicated needs to improve the easiness of connector & music-controller maintenance and the social acceptance of music-controller in MP3-YSJ 2.0. In MP3-YSS 2.0, the easiness of material management and the social acceptance of music controller & connector needed to improvement. In particular, users felt inconvenience in the social acceptance because of e-textile keypad arrangement. To outweigh this disadvantage, further study is needed about keypad interface of the music controller.

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The Validation of a Commercial Testosterone RIA Test Kits (Testosterone RIA 검사 kit 별 유효성 비교평가)

  • Ryu, Hwa-jin;Shin, Seon-yeong;Cho, Seong-uk
    • The Korean Journal of Nuclear Medicine Technology
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    • v.26 no.1
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    • pp.38-41
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    • 2022
  • Purpose Testosterone is a steroid hormone synthesized by the Leydig cells of the testes in men, and by the adrenal cortex and ovaries in women. Testosterone production is regulated by luteinzing hormone secreted by the anterior pituitary gland. In this experiment, the effectiveness of testosterone radioimmunoassay (RIA) kits produced by three companies was evaluated and compared in case the production of testosterone kits was stopped or supply problems occurred. Materials and Methods In October 2021, samples were collected from the patients (n=49) who requested the testosterone RIA test. The experiment was conducted by dividing the patient's sample into low concentration (1.0 ng/mL or less), medium concentration (2.0-4.0 ng/mL) and high concentration (6.0 ng/mL or more). The Testosterone RIA test compared and evaluated the validity of Company A kits used in this hospital and those of Company B and C used in other hospitals. The precision, sensitivity, recovery, linearity and correlation were evaluated for each kit. The testosterone RIA test was carried out in accordance with the insert kit manual for each manufacturer. Results As a result of measuring the precision of the intra assay, the Coefficient of Variation (CV) value of the company A kit was high at 11.4% only in the low concentration sample, and in the case of the company B and C kits, the CV value was less than 10% at low, medium, and high concentrations. In the inter-assay precision measurement, the CV value was less than 15% in both A and C kits, but in the case of the B kit, the CV value exceeded 15% at low and medium concentrations. Sensitivity was 0.13 ng/mL for company A, 0.01 ng/mL for company B, and 0.01 ng/mL for company C, and the linearity of all three kits showed excellent linearity. In the case of recovery rate, all of the A, B, and C company kits showed results that were out of 90-110%. In the case of correlation test, when compared with the company A kit currently use in here, the correlation coefficient (R2) value for the company B kit was 0.9508, and for the company C kit was 0.9352 Conclusion As a result, there was a slight difference in precision at the low concentration sample. The correlation test showed an excellent correlation coefficient. However, it was difficult to secure samples of various concentrations because there were not many tests of testosterone requested at this hospital. So, additional experiments should be carried out by acquiring samples of various concentrations on each laboratory later.

Impact of PSI-KIT Nitriding model on hypothetical Spent Fuel Pool accident simulation

  • Mateusz Malicki;Terttaliisa Lind
    • Nuclear Engineering and Technology
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    • v.55 no.7
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    • pp.2504-2515
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    • 2023
  • In past years the Paul Scherrer Institute (PSI, Switzerland) and the Karlsruhe Institue of Technology (KIT, Germany)) collaborated to develop a model to account for the active role of nitrogen in the air oxidation of a Zircalloy cladding. The "PSI-KIT Nitriding Model for Zirconium based Fuel Cladding" model was implemented at PSI into PSI-MELCOR 1.8.6. In order to make a preliminary evaluation of the effect of the new model on the evolution of full-scale spent fuel pool accidents, one spent fuel pool event was analyzed using the PSI research version of PSI-MELCOR 1.8.6, which includes the nitriding model. To adapt an existing input deck for the calculations, a sensitivity study was conducted to find an optimal nodalization for the analyses. The nitriding model results were compared to those calculated with the MELCOR 1.8.6-PSI without the new nitriding model. The results demonstrate the effect of the nitriding reactions in spent fuel pool accident progression. Moreover, they confirm the impact of ZrN formation during cladding oxidation in air when the oxidation reactions lead to oxygen starvation inside the fuel assemblies. The nitriding reaction led to higher chemical heat generation during the accident and to an earlier failure of the cladding than when the effect of nitrogen reactions was not considered. It should be noted that the nitriding model, as implemented in the PSI version of MELCOR 1.8.6 has not yet been conclusively validated. Thereby the results presented in this paper should be treated as a preliminary demonstration of the capabilities of the model.