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http://dx.doi.org/10.4014/mbl.1806.06006

A New ELISA Kit Based on Antigenic Epitopes for Diagnosing Brucella abortus  

Khodabakhsh, Tannaz (Department of Microbiology, School of Biology Sciences, North Branch of Islamic Azad University)
Arabi, Azin (Department of Microbiology, School of Biology Sciences, North Branch of Islamic Azad University)
Pakzad, Parviz (Department of Microbiology, School of Biology Sciences, North Branch of Islamic Azad University)
Gheflat, ShivaSadat (Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences)
Bahreinipour, Akram (Iran Veterinary Organization (IVO))
Bandehpour, Mojgan (Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences)
Publication Information
Microbiology and Biotechnology Letters / v.47, no.1, 2019 , pp. 158-163 More about this Journal
Abstract
Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity.
Keywords
Brucella abortus; protein expression; antigenic epitopes; VirB2.3.9; ELISA;
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