• Asian-Australasian Journal of Animal Sciences
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• v.26 no.5
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• pp.711-715
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• 2013

To investigate the effect of restricted grazing time on behavior of Tan sheep on desert steppe, forty 4-months old male Tan sheep with an original body weight (BW) of $15.62{\pm}0.33$ kg were randomly allocated to 4 grazing groups which corresponded to 4 different restricted grazing time treatments of 2 h/d (G2), 4 h/d (G4), 8 h/d (G8) and 12 h/d (G12) access to pasture. The restricted grazing times had a significant impact on intake time, resting time, ruminating time, bite rate and movement. As the grazing time decreased, the proportion of time spent on intake, bite rate and grazing velocity significantly (p<0.05) increased, but resting and ruminating time clearly (p<0.05) decreased. The grazing months mainly depicted effect on intake time and grazing velocity. In conclusion, by varying their foraging behavior, Tan sheep could improve grazing efficiency to adapt well to the time-limited grazing circumstance.

• Journal of Microbiology and Biotechnology
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• v.18 no.10
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• pp.1630-1633
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• 2008

A novel putative toxin-antitoxin segregational stability system named KyAB system was identified in a novel native plasmid pBMB8240 from Bacillus thuringiensis strain YBT-1520, based on sequences homology with other toxin-antitoxin systems, the lethal activity of the KyB putative toxin in Escherichia coli and the stabilizing effect of the kyAB system in Bacillus thuringiensis. Secondarily, the native plasmid pBMB9741 from the same strain was resequenced and the corrected plasmid was named as pBMB7635. Based on sequence homology with the tasAB system and the lethal activity of toxin protein in Escherichia coli, a tasAB-like putative toxin-antitoxin system was identified on pBMB7635.

• Carbon letters
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• v.14 no.1
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• pp.45-50
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• 2013

Polyacrylonitrile (PAN) copolymers of different molecular weights were synthesized by a suspension polymerization and precipitation polymerization method. The rheology behaviors of the synthesized PAN copolymers were investigated in relation to their molecular weight, solid content and melting temperature. The influence of "historical effects" on the spinning solution of PAN was studied by analyzing the laws of viscosity considering the diversification time and temperature. The viscosity disciplines of each spinning solution conformed well to the rheological universal laws in a comparison of the suspension polymerization product with that of precipitation polymerization. Viscosity changes in the swelling process of dissolution were gentler in the suspension polymerization product; a small amount of water will quickly debase the solution viscosity, and high-speed mixing can greatly shorten the time required by the spinning solution to reach the final viscosity.

• Journal of Power Electronics
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• v.18 no.5
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• pp.1470-1478
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• 2018

Recently, wireless power transfer (WPT) via coupled magnetic resonances has attracted a lot of attention owing to its long operation distance and high efficiency. However, the WPT systems is over-coupling and a frequency splitting phenomenon occurs when resonators are placed closely, which leads to a decrease in the transfer power. To solve this problem, an adaptive frequency tracking control (AFTC) was used based on a closed-loop control scheme. An improved particle swarm optimization (PSO) algorithm was proposed with the AFTC to track the maximum power point in real time. In addition, simulations were carried out. Finally, a WPT system with the AFTC was demonstrated to experimentally validate the improved PSO algorithm and its tracking performance in terms of optimal frequency.

• Journal of Power Electronics
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• v.19 no.3
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• pp.827-834
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• 2019

Wireless power transfer via coupled magnetic resonances has been a hot research topic in recent years. In addition, the number of related devices has also been increasing. However, reverse signals transfer is often required in addition to wireless power transfer. The structure of the circuit for a wireless power transfer system via coupled magnetic resonances is analyzed. The advantages and disadvantages of both parallel compensation and series compensation are listed. Then the compensation characteristics of the inductor, capacitor and resistor were studied and an appropriate compensation method was selected. The reverse signals can be transferred by controlling the compensation of the resistor. In addition, it can be demodulated by extracting the change of the primary current. A 3.3 MHz resonant frequency with a 100 kHz reverse signals transfer system platform was established in the laboratory. Experimental results demonstrate that wireless power and reverse signals can be transferred synchronously.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.740-746
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• 2021

Efficient cellulolytic enzyme production is important for the development of lignocellulose-degrading enzyme mixtures. However, purification of cellulases from their native hosts is time- and labor-consuming. In this study, a constitutive expression system was developed in Penicillium oxalicum for the secreted production of proteins. Using a constitutive polyubiquitin gene promoter and cultivating with glucose as the sole carbon source, nine cellulolytic enzymes of different origins with relatively high purity were produced within 48 h. When supplemented to a commercial cellulase preparation, cellobiohydrolase I from P. funiculosum and cellobiohydrolase II from Talaromyces verruculosus showed remarkable enhancing effects on the hydrolysis of steam-exploded corn stover. Additionally, a synergistic effect was observed for these two cellobiohydrolases during the hydrolysis. Taken together, the constitutive expression system provides a convenient tool for the production of cellulolytic enzymes, which is expected to be useful in the development of highly efficient lignocellulose-degrading enzyme mixtures.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1043-1052
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• 2019

Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with $p-NPC_{16}$ at a pH of 8.5 and $50^{\circ}C$, and the $K_m$, $k_{cat}$, and $k_{cat}/K_m$ values were 1.05 mM, $292.95s^{-1}$ and $279s^{-1}mM^{-1}$, respectively. The lipase was highly stable at $7.5{\leq}pH{\leq}10.0$. $K^+$ and $Na^+$ exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.

• Steel and Composite Structures
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• v.49 no.5
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• pp.517-532
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• 2023

Tower structures have been widely used in communication and transmission engineering. The failure of joints is the leading cause of structure failure, which make it play a crucial role in tower structure engineering. In this study, the aluminum alloy three tube tower structure is taken as the prototype, and the middle joint of the tower was selected as the research object. Three different stainless steel-aluminum alloy composite joints (SACJs), denoted by TA, TB and TC, were designed. Finite element (FE) modeling analysis was used to compare and determine the TC joint as the best solution. Detail requirements of fasteners in the TC stainless steel-aluminum alloy composite joint (TC-SACJ) were designed and verified. In order to systematically and comprehensively study the mechanical properties of TC-SACJ under multi-directional loading conditions, the full-scale experiments and FE simulation models were all performed for mechanical response analysis. The failure modes, load-carrying capacities, and axial load versus displacement/stain testing curves of all full-scale specimens under tension/compression loading conditions were obtained. The results show that the maximum vertical displacement of aluminum alloy tube is 26.9mm, and the maximum lateral displacement of TC-SACJs is 1.0 mm. In general, the TC-SACJs are in an elastic state under the design load, which meet the design requirements and has a good safety reserve. This work can provide references for the design and engineering application of aluminum alloy tower structures.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.3
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• pp.1144-1156
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• 2014

In Internet, IP multicast has been used successfully to provide an efficient, best-effort delivery service for group communication applications. However, applications such as multiparty private conference, distribution of stock market information, pay per view and other subscriber services may require secure multicast to protect integrity and confidentiality of the group traffic, and validate message authenticity. Providing secure multicast for group communication is problematic without a robust group key management. In this paper, we propose a group key management scheme based on the secret sharing technology to require each member by itself to generate the group key when receiving a rekeying message multicast by the group key distributor. The proposed scheme enforces mutual authentication between a member and the group key distributor while executing the rekeying process, and provides forward secrecy and backward secrecy properties, and resists replay attack, impersonating attack, group key disclosing attack and malicious insider attack.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.3
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• pp.1246-1259
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• 2015

Certificate-based signature (CBS) combines the advantages of both public key-based signature and identity-based signature, while saving from the disadvantages of drawbacks in both PKS and IBS. The insecure deployment of CBS under the hostile circumstances usually causes the exposure of signing key to be inescapable. To resist the threat of key leakage, we present a pairing-free key insulated CBS scheme by incorporating the idea of key insulated mechanism and CBS. Our scheme eliminates the costly pairing operations and as a matter of fact outperforms the existing key insulated CBS schemes. It is more suitable for low-power devices. Furthermore, the unforgeability of our scheme has been formally proven to rest on the discrete logarithm assumption in the random oracle model.