• Mycobiology
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• v.42 no.2
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• pp.114-119
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• 2014

The wood decay fungi Antrodia P. Karst. play important ecological roles and have significant industrial and economic impacts as both wood degraders and sources of pharmaceutical and biotechnological products. Although each Antrodia species has distinct morphological characteristics, the misidentification rate is especially high due to their simple morphological characters. A combination of morphological and internal transcribed spacer region sequence analyses revealed that 27 of 89 specimens previously identified by morphology alone were correct, whereas 35 of these specimens were misidentified as other Antrodia species. We report here that seven Antrodia species exist in Korea (A. albida, A. heteromorpha, A. malicola, A. serialis, A. sinuosa, A. sitchensis, and A. xantha) and based on these specimens, we provide taxonomic descriptions of these species, except for A. serialis, which was only confirmed by isolate.

• Advances in Traditional Medicine
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• v.5 no.1
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• pp.62-68
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• 2005

Doxorubicin is a powerful anticancer antibiotic extensively used in the treatment of several types of cancers. Long-term administration of this drug results in cumulative dose related cardiotoxicity due to enhanced production of free radicals leading to oxidative stress. Our earlier investigations have demonstrated significant antioxidant, anti-inflammatory and antitumour properties of Ganoderma lucidum extracts. We extended our investigations to evaluate the protective effect of Ganoderma lucidum extract against doxorubicin-induced cardiotoxicity. Administration of 3 doses of doxorubicin, 6 mg/kg body weights, i.p. per each dose, alternative days, showed dear signs of cardiotoxicity in rats. The drug enhanced serum creatine kinase (CK) activity and lipid peroxidation in tissue drastically. The drug also induced significant decrease in GSH level and activities of CAT, SOD and GPx. Administration of methanolic extract of G.lucidum (500 and 1,000 mg/kg body weight) significantly increased the level of GSH and activities of CAT, SOD and GPx. Activity of CK was significantly lowered in a dose dependent manner. The treatment also caused significant decrease in lipid peroxidation (MDA). The results thus indicated that methanolic extract of G.lucidum prevented oxidative stress caused by doxorubicin administration and the increase in serum CK activity and lipid peroxidation in the tissue. The experimental findings suggest the therapeutic potential of G.lucidum as adjuvant in cancer chemotherapy.

• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.2
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• pp.187-193
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• 1990

Ganoderma lucidum has been widely used not only as ingredients in herbal medicine but also in pharmacological soft drinks. The author collected for analysis of content of 8 kinds of heavy metal(Cd, pb, Hg, Cu, Mn, Fe, As) in soil and cluture soil in an around the Taegu area including Sang Ju, Non Gong, Keum Ho, and Weol Bae. THe toxic content in Gaoderma lucidum showed relatively low level as in cadmium lead mercury arsenic : 0.8-0.13ppm 0.17-1.43ppm 0.02-0.32ppm 0.01-0.19ppm respectively : in copper mangenese zinc and iron : 0.93-4.29ppm, 0.37-2.18ppm 1.02-1.65ppm, 4.57-11.04ppm those grown in soil showed higher percentages of content than those grown on logs in lead copper zinc and iron by 43.2% 68.6%, 20.3% and 43.2% respectively. The content of heavy metals in those grown in soil and culture soil tended to be higher in the areas near factories of industrial complexes especially in manganese and iron. The content of heavy metals in soil and culture soil appeared lower than the mean values of Korean Soil. No interrelationship was found in the content of heavy metals between those of Ganoderma lucidum grown on logs and those grown in soil. In case of pot cultivation however the mushroom spawns are grown originally in soil which seems to influence the degree of content of heavy metals of media.

• Journal of the Speleological Society of Korea
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• no.66
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• pp.45-57
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• 2005

This Paper is a study on the duality of speleothem that appeared in 'Hyeob Jae Cave' which is designated as the natural monumen. No.236. It is located at Hyeob Jae Ri, Hanrim Eub, Bug Je-ju Gun, Je-judo. The findings are as follows. 1. The distribution range of the shelly sand which has maximum thickness of $10m{\pm}$ and average of $3m{\pm}$ was $3.2m^2$. 2. The desert hollow acted to promote the speleothem deposits in the lava tunnel with lava mound formed by lavapilz and artificial breaksand wall. 3. The main component of the pseudo limestone cavern was carbonate Calcium from shelly sand. And the deposition of speleothem in the Cave was accelerated after the volcanic erupsion of Biyang island in 1002. A.D. 4. The secondary depositions of Calcite recognized as speleothem up to now it can be used for the pseudo karst in general. 5. It seems that the variety of the cave deposits is decided depending upon the geology, land form, climate, vegetations and the structural environmental factors. 6. It seems that the wondering development of accretionary deposits caused by encrusting has a close relation with intermittent seepage of ground water. 7. Finally, we can acknowledge the coexistent duality of speleothem by shelly sand along with the joint and the lava stalactites formed at the same time with the lava tunnel on the ceiling where there was no seepage.

• Korean Journal of Pharmacognosy
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• v.30 no.1
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• pp.25-33
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• 1999

In order to find less toxic antiviral agents from basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers.) Karst. EA was examined for antiviral activity against five strains of pathogenic viruses such as encephalomyocarditis virus (EMCV), vesicular stomatitis virus (VSV) Indiana and New Jersey strains, influenza A virus (Flu A), and varicella zoster virus (VZV) in vitro. Antiviral activity was evaluated by plaque reduction assay. Among five strains of viruses tested, EA exhibited the most potent antiviral activity against VSV Indiana strain with 50% effective concentration $(EC_{50})$ of 0.104 mg/ml in Vero cells, and its selectivity index (SI) was 36.5. EA was also examined for the virucidal activity, antiviral activity in preincubation on VSV Indiana strain in order to examine possible mode of antiviral activity. Preincubation of Vero cells with EA did not confer protection against VSV, however, prolonged exposure of cells to EA inhibited the replication of virus dose-dependently. In virucidal activity, the titer of infectious virus did not decrease significantly.

• Mycobiology
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• v.29 no.1
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• pp.11-14
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• 2001

A preparation of water soluble components(EA) was made from carpophores of Elfvingia applanata(Pers.) Karst and its in vitro antibacterial activity on a number of bacterial species was examined by macrobroth dilution assay. Among 16 species of bacteria tested, the most potent antibacterial activity was observed against Staphylococcus epiderrnidis and Proteus vulgaris, of which MICs were 1.25 mg/ml. To investigate the antibacterial effects in combinations of EA with quinolone antibiotics, such as ciprofloxacin, enoxacin, lomefloxacin, norfloxacin, and ofloxacin, the fractional inhibitory concentrations(FICs) and the fractional inhibitory concentration indices(FICIs) for four bacterial strains were determined by macrobroth dilution checkerboard assay. Combinations of EA and quinolones exhibited either additive or indifferent effects of antibacterial activity in most instances. However, both synergistic and antagonistic effects were not observed in any cases.

• Korean Journal of Pharmacognosy
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• v.30 no.3
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• pp.244-249
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• 1999

In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata(Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [Indiana serotype, VSV(IND)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha or gamma were examined on the multiplication of VSV(IND). EA caused a concentration-dependent reduction in the plaque formation of VSV(IND) with 50% effective concentration $(EC_{50})$ of $104.02\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. All cases of the combination of EA with IFN alpha or IFN gamma showed potent synergism with CI values of $0.38{\sim}0.52$ for $50{\sim}90%$ effective levels.

• Mycobiology
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• v.36 no.1
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• pp.28-33
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• 2008

Ganoderma lucidum (Fr.) Karst (Polyporaceae), belonging to basidiomycota, is one of the most famous medicinal mushrooms. This study was carried out to investigate favorable mycelial growth conditions, such as pH, temperature, growth media, carbon sources and nitrogen sources of Korean strains in G. lucidum. The most suitable temperature for the mycelial growth was obtained at $30^{\circ}C$. In general, optimal temperature range for the mycelial growth was found at $25{\sim}30^{\circ}C$. This Mushroom has a broad pH range ($5{\sim}9$) for its mycelial growth and mostly favorable growth was found at pH 5. Generally, Hamada, Glucose peptone, YM, Mushroom complete and Lilly media were the most suitable for the mycelial growth of G. lucidum. Among 10 different carbon sources, dextrin, galactose and fructose were best but the rest of other carbon sources also facilitated the growth of mycelia. The most suitable nitrogen sources were ammonium acetate, glycine, arginine and calcium nitrate, but to a certain extent, all of the supplemented nitrogen sources also stimulated the mycelial growth.

• Geomechanics and Engineering
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• v.25 no.1
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• pp.59-73
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• 2021

There frequently exists inadequacy regarding the number of boreholes installed along tunnel alignment. While geophysical imaging techniques are available for pre-tunnelling geological characterization, they aim to detect specific object (e.g., water body and karst cave). There remains great motivation for the industry to develop a real-time identification technology relating complex geological conditions with the existing tunnelling parameters. This study explores the potential for the use of machine learning-based data driven approaches to identify the change in geology during tunnel excavation. Further, the feasibility for machine learning-based anomaly detection approaches to detect the development of clayey clogging is also assessed. The results of an application of the machine learning-based approaches to Xi'an Metro line 4 are presented in this paper where two tunnels buried in the water-rich sandy soils at depths of 12-14 m are excavated using a 6.288 m diameter EPB shield machine. A reasonable agreement with the measurements verifies their applicability towards widening the application horizon of machine learning-based approaches.

• Geomechanics and Engineering
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• v.38 no.1
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• pp.69-77
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• 2024

The development of shield-driven cross-river tunnels in China is witnessing a notable shift towards larger diameters, longer distances, and higher water pressures due to the more complex excavation environment. Complex geological formations, such as fault and karst cavities, pose significant construction risks. Real-time adjustment of shield tunneling parameters based on parameter prediction is the key to ensuring the safety and efficiency of shield tunneling. In this study, prediction models for the torque and thrust of the cutter plate of ultra-large diameter slurry shield TBMs is established based on integrated learning algorithms, by analyzing the real data of Heyan Road cross-river tunnel. The influence of geological complexities at the excavation face, substantial burial depth, and high water level on the slurry shield tunneling parameters are considered in the models. The results reveal that the predictive models established by applying Random Forest and AdaBoost algorithms exhibit strong agreement with actual data, which indicates that the good adaptability and predictive accuracy of these two models. The models proposed in this study can be applied in the real-time prediction and adaptive adjustment of the tunneling parameters for shield tunneling under complex geological conditions.