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http://dx.doi.org/10.4489/MYCO.2008.36.1.028

Favorable Culture Conditions for Mycelial Growth of Korean Wild Strains in Ganoderma lucidum  

Jayasinghe, Chandana (Department of Biology, University of Incheon)
Imtiaj, Ahmed (Department of Biology, University of Incheon)
Hur, Hyun (Department of Biology, Dongguk University)
Lee, Geon-Woo (Department of Biology, University of Incheon)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Lee, U-Youn (Department of Biology, University of Incheon)
Publication Information
Mycobiology / v.36, no.1, 2008 , pp. 28-33 More about this Journal
Abstract
Ganoderma lucidum (Fr.) Karst (Polyporaceae), belonging to basidiomycota, is one of the most famous medicinal mushrooms. This study was carried out to investigate favorable mycelial growth conditions, such as pH, temperature, growth media, carbon sources and nitrogen sources of Korean strains in G. lucidum. The most suitable temperature for the mycelial growth was obtained at $30^{\circ}C$. In general, optimal temperature range for the mycelial growth was found at $25{\sim}30^{\circ}C$. This Mushroom has a broad pH range ($5{\sim}9$) for its mycelial growth and mostly favorable growth was found at pH 5. Generally, Hamada, Glucose peptone, YM, Mushroom complete and Lilly media were the most suitable for the mycelial growth of G. lucidum. Among 10 different carbon sources, dextrin, galactose and fructose were best but the rest of other carbon sources also facilitated the growth of mycelia. The most suitable nitrogen sources were ammonium acetate, glycine, arginine and calcium nitrate, but to a certain extent, all of the supplemented nitrogen sources also stimulated the mycelial growth.
Keywords
Culture condition; Ganoderma lucidum; Media; Mycelial growth; Nutrition;
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1 Akinyele, B. J. and Adetuyi, F. C. 2005. Effect of agrowastes, pH and temperature variation on the growth of Volvariella volvacea. Afr. J. Biotech. 4:1390-1395
2 Chandra, A. and Purkayastha, R. P. 1977. Physiological studies on Indian mushrooms. Trans. Br. Mycol. Soc. 69:63-70   DOI
3 Lee, K. M., Lee, S. Y. and Lee, H. Y. 1999a. Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an air-lift fermentor. J. Biosci. Bioeng. 88:646-650   DOI   ScienceOn
4 Lee, I. K., Shim, H. J., Woo, S. D., Je, Y. H., Yang, Z. and Kang, S. K. 1999b. Variation in growth and pathogenicity of Beauveria barssiana and Paecilomyces fumosoroseus pathogenic to the pine gall midge, Thecodiplosis japonensis. Kor. J. Appl. Micro. Biotech. 27:415-418
5 Kino, K., Yamashita, A., Yamaoka, K., Watanabe, J., Tanaka, S., Ko, K., Shimizu, K. and Tsuno, H. 1989. Isolation and characterization of a new immunomodulatory protein, Ling Zhi-8 (LZ-8), from Ganoderma lucidium. J. Biol. Chem. 264:472-478
6 Kuforiji, O. O. and Fasidi, I. O. 1998. Growth requirement of Pleuroteus tuberregium, a Nigerian mushroom. Proc. Nigerian Soci. Micro. 1:73-78
7 Jonathan, S. G., Fasidi, I. O. and Ajayi, E. J. 2004. Physico-chemical studies on Volvariella esculent Mass (Singer), a Nigerian edible fungus. Food Chem. 85:339-342   DOI   ScienceOn
8 Jonathan, S. G. and Fasidi, I. O. 2003. Studies on Psathyrella atroumbonatai (Pegler), a Nigerian edible fungus. Food Chem. 81:481-484   DOI   ScienceOn
9 Jonathan, S. G. 2002. Vegetative growth requirements and antimicrobial activities of some higher fungi in Nigeria. Ph. D. Thesis. University of Ibadan, Ibadan, Nigeria
10 Hikino, H. and Mizuno, T. 1989. Hypoglycemic actions of some heteroglycans of Ganoderma lucidum fruit bodies. Planta Medica 55:385-389   DOI   ScienceOn
11 Adejoye, O. D., Adebayo-Tayo, B. C., Ogunjobi, A. A. and Afolabi, O. O. 2007. Physicochemical studies on Schizophyllum commune (Fries), a Nigerian edible fungus. World Appl. Sci. J. 2:73-76
12 Morrison, R. and Boyd, R. N. 1992. Carbohydrates I. Monosaccharides. In: Organic Chemistry, pp. 1144-1146. Englewood Cliffs, Prentice Hall. New Jersey
13 Yang, F. C., Ke, Y. F. and Kuo, S. S. 2000. Effect of fatty acids on the mycelia growth and polysaccharide formation by Ganoderma lucidum in shake flask cultures. Enz. Microb. Technol. 27:295-301   DOI   ScienceOn
14 Yang, F. C. and Liau, C. B. 1998. The influence of environmental conditions on polysaccharide formation by Ganoderma lucidum in submerged fermentations. Process. Biochem. 33:547-553   DOI   ScienceOn
15 Tsujikura, Y., Higuchi. T., Miyamoto, Y. and Sato, S. 1992. Manufacture of ganoderic acid by fermentation of Ganoderma lucidum. Jpn. Kokai Tokkyo Koho. JP04304890
16 Fasola, T. R., Gbolagade, J. S. and Fasidi, I. O. 2007. Nutritional requirements of Volvariella speciosa (Fr. Ex. Fr.) Singer, a Nigerian edible mushroom. Food Chem. 100:904-908   DOI   ScienceOn
17 Griffin, D. H. 1994. Chemical requirement for growth. In: Fungal Physiology, 2nd edition, pp. 130-157. Wiley Liss. New York
18 Garraway, O. M, and Evans, C. R. 1984. Fungal Nutrition and Physiology. John Wiley and Sons, New York
19 Lindequist, U., Lesnau, A., Teuscher, E. and Pilgrim, H. 1989. Untersuchungen zur antiviralen Wirksamkeit von Ergosterolperoxid. Pharmazie 44:579-580
20 Mizuno, T., Wang, G., Zhang, J., Kawagishi, H., Nishitoba, T. and Li, J. 1995. Reishi, Ganoderma lucidum and Ganoderma tsugae: bioactive substances and medicinal effects. Food Rev. Intl. 11:151-166   DOI   ScienceOn
21 Shim, J. O., Son, S. G., Kim, Y. H., Lee, Y. S., Lee, J. Y., Lee, T. S., Lee, S. S. and Lee, M. W. 1997. The culture conditions affecting the mycelial growth of Grifola umbellata. Kor. J. Mycol. 25:209-218   과학기술학회마을
22 Shim, S. M., Lee, K. R., Kim, S. H., Im, K. H., Kim, J. W., Lee, U. Y., Shim, J. O., Lee, M. W. and Lee, T. S. 2003. The optimal culture conditions affecting the mycelial growth and fruiting body formation of Paecilomyces fumosoroseus. Mycobiology 31:214-220   과학기술학회마을   DOI
23 Sung, J. M., Kim, C. H., Yang, K. J., Lee, H. K. and Kim, Y. S. 1993. Studies on distribution and utilization of Cordyceps militaris and C. nutans. Kor. J. Mycol. 21:94-105   과학기술학회마을
24 Shim, S. M., Oh, Y. H., Lee, K. R., Kim, S. H., Im, K. H., Kim, J. W., Lee, U. Y., Shim, J. O., Shim, M. J., Lee, M. W., Ro, H. S., Lee, H. S. and Lee, T. S. 2005. The characteristics of culture conditions for the mycelial growth of Macrolepiota procera. Mycobiology 33:15-18   과학기술학회마을   DOI
25 Kim, H. K., Park, J. S., Cha, D. Y., Kim, Y. S. and Moon, B. J. 1994. Study on the artificial cultivation of Lentinus lepideus (Fr. ex. Fr.). Kor. J. Mycol. 22:145-149   과학기술학회마을
26 Kohda, H., Tokumoto, W., Sakamoto, K., Fujii, M., Hirai, Y., Yamasaki, K., Komoda, Y., Nakamura, H., Ishihara, S. and Uchida, M. 1985. The biologically active constituents of Ganoderma lucidum (Fr.) Karst. histamine release-inhibitory triterpenes. Chem. Pharm. Bull. 33:1367-1374   DOI   ScienceOn
27 Tasaka, K., Akagi, M., Miyoshi, K., Mio, M. and Makino, T. 1988. Antiallergic constituents in the culture medium of Ganoderma lucidum. (I) Inhibitory effect of cyclooctasulfur on histamine release. Agents Actions 23:153-156   DOI
28 Toth, J. O., Luu, B. and Ourisson, G., 1983. Ganoderic acid T and Z: cytotoxic triterpenes from Ganoderma lucidum. Tetrahedron Lett. 24:1081-1084   DOI   ScienceOn