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Studies of Insecticide Resistance in the Green Peach Aphid,_Myzus persicae (Sulz.) (IV). Oxydemeton-methyl Resistance Devlopment, Cross Resistance and Esterase Isozymes (복숭아혹진딧물의 살충제저항성에 관(關)한 연구(硏究)(IV) -Oxydemeton-methyl 도태(淘汰)에 의(依)한 저항성발달(抵抗性發達), 교차저항성(交叉抵抗性) 및 Esterase Isozymes-)

  • Choi, Seung-Yoon;Kim, Gil-Hah
    • Korean journal of applied entomology
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    • v.25 no.3 s.68
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    • pp.151-157
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    • 1986
  • Population of the green peach aphid, Myzus persicae(Sulz.), were successively selected(up to 20th generation) in the laboratory by the insecticide oxydemeton-methyl (Metasystox-R 25Ec) and the resistance was linearly increased with the selected generations. The population developed 355.5-to 359.9-fold resistance to oxydemeton-methyl at 20th generation-selection. The population showed cross resistance to the insectides cypermethrin(770.1-to 778.5-fold), acephate(25.6-to 33.2-fold) and pirimicarb(3.4-to 3.6-fold). The different esterase isozymes were detected by the electrophoresis from the selected and non-selected strains and the band of the ${\beta}-2$ from the selected strain showed greater esterase activity than that of the non. selected strain.

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First-principles Study on the Half-metallicity and Magnetism of the (001) Surfaces of (AlP)1/(CrP)1 Superlattice ((AlP)1/(CrP)1 초격자계에서 (001) 표면의 자성과 반쪽금속성에 대한 제일원리 연구)

  • Bialek, Beata;Lee, Jae Il
    • Journal of the Korean Magnetics Society
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    • v.25 no.6
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    • pp.175-179
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    • 2015
  • The half-metallicity and magnetism of the (001) surfaces of $(AlP)_1/(CrP)_1$ superlattice were investigated by means of FLAPW (Full-potential Liniarized Augmented Plane Wave) method. We considered four types of (001) surface termination, i.e., Al(S)-, Cr(S)-, P(S)Al(S-1)- and P(S)Cr(S-1)-term systems. We found that only Cr(S)-term system maintains the half-metallicity at the surface as only this system has the calculated magnetic moment of integer number of bohr magnetons. The magnetic moment of Cr(S) atom in the system was $3.02{\mu}_B$ which was increased from the bulk value by the effects of band narrowing and increased spin-splitting at the surface. The electronic density of states of the P(S) atom in the P(S)Al(S-1)-term showed very sharp surface states due to the broken $p_z$ bonds at the surface. We found there is still a strong p-d hybridization between the P(S) and Cr(S-1) layers in the P(S)Cr(S-1)-term which causes a considerable increase of magnetic moment of P(S) atom.

Characteristics of Polysaccharide Extracted from the Cultured Mycelia of Schizophyllum commune (치마버섯 균사체 배양물로부터 분리한 다당류의 이화학적 특성)

  • Lee, June-Woo;Kim, Yong-Seok
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.91-95
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    • 2009
  • To examine physico-chemical properties of the polysaccharide extracted from liquid-cultured mycelia of Schizophyllum commune, each the polysaccharide was extracted with hot water treatment and then fractionated with ethanol, alkaline solution and ultrafiltration. And we determined carbohydrate contents, composition of amino acids, infra-red spectrum and viscosity. Carbohydrate contents of polysaccharide treated with ethanol and ultrafiltration were 72.0% and 62.3%, and proteins content were 15.3% and 32.0% respectively. The carbohydrate consisted of four monosaccharides and the protein contained 16 amino acids. The polysaccharide obtained from ultrafiltration was shown an absorption band characteristic of the ${\beta}$-glycosidic linkage by infra red spectra. These results suggest that the polysaccharide extracted from Schizophyllum commune showed the characteristics of proteinbounded polysaccharide, and it was ${\beta}$-glycosidic linkage with strong viscosity.

Effect of Ground Roll Suppression Based on Karhunen-Loeve Transform (카루넨-루베 변환을 이용한 탄성파 그라운드 롤 억제 효과)

  • Jang, Seonghyung;Lee, Donghoon
    • Geophysics and Geophysical Exploration
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    • v.22 no.4
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    • pp.177-185
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    • 2019
  • Ground roll is a surface wave which is usually observed in the land seismic data. It is one of the typical coherent noise. During the reflection data processing, ground roll is removed because it is considered as noise. This removal process often causes the loss of reflection signals if the ground roll overlaps reflection signals. In this study, we look over Karhunen-Loeve Transform (KLT) and analyze its effects to suppress the ground roll appropriately while reducing the reflection loss. Numerical tests in homogeneous elastic media show that the ground roll has been properly rejected. However, the field data application reveals that there is no significant suppression of ground roll when compared to band-pass filtering. This can be considered that it is hard to calculate horizontally aligned gathers in the field data because the ground roll contains a wide range of frequency bands. On the contrary, the result of singular value decomposition (SVD) filtering shows that the ground roll has been significantly reduced. It is thought that the SVD filtering performs better in the ground roll suppression than KLT because it is easy to calculate the horizontally aligned gathers in the SVD filtering.

산화철 폐촉매를 사용한 NiZn-페라이트웨 합성 및 자기적 특성

  • Park, Sang-Il;Hwang, Yeon;Lee, Hyo-Sook
    • Proceedings of the Korea Crystallographic Association Conference
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    • 2003.05a
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    • pp.26-26
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    • 2003
  • 산업 폐기물로서 전량 매립되고 있는 styrene monomer (SM) 공정에서 발생되는 산화철 폐촉매를 사용하여 NiZn-페라이트를 합성하였고, 그 자기적 특성을 조사하였다. 평균입경 0.5㎛로 미분쇄된 산화철 폐촉매에 NiO 및 ZnO를 혼합하여 900℃에서 하소한후 1230℃에서 5시간 동안 소결하여 스핀넬형 페라이트 소결체를 얻었다. Ni/sub x/Zn/ub 1-x/Fe₂O₄(x=0.36, 0.50, 0.66) 및 (Ni/sub 0.5/Zn/sub 0.5/)/sub 1-y/Fe/sub 2+y/O₄(y=-0.02, 0, 0.02) 조성에 대하여 초투자율을 측정하였다. S-parameter를 측정하여 반사 감쇄량을 계산하였다. Ni/sub 0.5/Zn/sub 0.5/fe₂O₄ 조성의 경우 밀도 5.38 g/㎤ kHz에서의 초투자율이 59인 특성을 얻었다. 산화철 폐촉매를 이용하여 X-band 주파수 영역에서 높은 전자파 흡수능을 갖는 전파흡수체를 제조할 수 있음을 확인하였다. Ni/sub 0.5/Zn/sub 0.5/Fe₂O₄ 조성에 대하여 1100℃에서 하소한 분말을 사용하여 실리콘 고무에 복합시킨 후 전파흡수능을 측정하였다. 폐촉매에는 산화철 이외에 CeO₂가 주된 첨가물로 함유되어 있어서 페라이트의 합성 후에도 2차상으로서 존재하였다. 출발 원료인 산화철 폐촉매에 존재하는 K₂O 및 CeO₂를 제거하기 위하여 산처리와 분산제를 이용한 CeO₂ 분리과정을 행하였다. 정제된 산화철 폐촉매에 NiO 및 ZnO를 혼합하여 900℃에서 하소하여 스핀넬형 페라이트를 합성하고 1325℃에서 5시간 소결하였다. 위와 마찬가지로 Ni/sub x/Zn/sub 1-x/Fe₂O₄(x=0.36, 0.50, 0.66)과 (Ni/sub 0.5/Zn/sub 0.5/)/sub 1-y/Fe/sub 2+y/O₄(y=-0.02, 0, 0.02) 조성에 대하여 자기적 특성을 조사하였다.화된 중성자빔으로 측정하였다 BPC-Si를 구부려 슬랩의 곡률반경을 변화시키면서 단색기-시료-검출기가 평행파 반평행배치일 때 Cu(111), (200), (220), (311), (331), (420)면의 회절선을 측정하여 각 조건에서 분해능과 강도를 평가하였다.료의 가시적 변화를 통해 illumination angle 1.25mrad(Dose rate : 334 × 10³ e/sup -//sec·n㎡)일 경우 약 3초 이내에 비정질화가 시작됨을 알 수 있었고 이는 약 1 × 10/sup 6/ e/sup -//sec·n㎡ 의 전자선량에 해당되며 이를 기준으로 각각의 illumination angle에 대한 임계전자선량을 평가할 수 있었다. 실질적으로 Cibbsite와 같은 무기수화물의 직접가열실험 시 전자빔 조사에 의해 야기되는 상전이 영향을 배제하고 실험을 수행하려면 illumination angle 0.2mrad (Dose rate : 8000 e/sup -//sec·n㎡)이하로 관찰하고 기록되어야 함을 본 자료로부터 알 수 있었다.운동횟수에 의한 영향으로써 운동시간을 1일 6시간으로 설정하여, 운동횟수를 결정하기 위하여 오전, 오후에 각 3시간씩 운동시키는 방법과 오전부터 6시간동안 운동시키는 두 방법을 이용하여 품질을 비교하였다. 각 조건에 따라 운동시킨 참돔의 수분함량을 나타낸 것으로, 2회(오전 3시간, 오후 3시간)에 나누어서 운동시키기 위한 육의 수분함량은 73.37±2.02%를 나타냈으며, 1회(6시간 운동)운동시키기 위한 육은 71.74±1.66%을 나타내었다. 각각의 운동조건에서 양식된 참돔은 사육초기에는 큰 변화가 없었으나, 사육 5일 이후에는 수분함량이 증가하여 15일에는 76.40±0.14, 75.62±0.98%의 수분함량을 2회와 1회 운동시킨 참돔의 육에서 각각 나타났다. 운동횟수에 따른 지질함량은 2회 운동시킨 참돔은 5.83±2.08, 1회 운

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Pseudomonas aeruginosa Exotoxin A Induces Apoptosis in Chemoresistant YD-9 Human Oral Squamous Carcinoma Cell Line Via Accumulation of p53 and Activation of Caspases (항암제에 저항성을 가지는 YD-9 human oral squamous carcinoma cell line에서 Pseudomonas aeruginosa exotoxin A의 p53 단백질 누적과 caspase를 활성화 경로를 통해 유도된 세포자멸사)

  • Kim, Gyoo-Cheon;Gil, Young-Gi
    • Journal of Life Science
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    • v.19 no.8
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    • pp.1047-1054
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    • 2009
  • Oral squamous carcinoma (OSC) cells present resistance to chemotherapeutic agents-mediated apoptosis in the late stages of malignancy. Advances in the understanding of bacterial toxins have produced new strategies for the treatment of cancers. It was demonstrated here that Pseudomonas aeruginosa exotoxin A (PEA) significantly decreased the viability of chemoresistant YD-9 cells in the apoptosis mechanism. Apoptotic manifestations were evident through changes in nuclear morphology and generation of DNA fragmentation. PEA treatment induced caspase-3, -6 and -9 cleavage, and activation. These events preceded proteolysis of the caspase substrates poly (ADP-ribose) polymerase (PARP), DNA fragmentation factor 45 (DFF45), and lamin A in YD-9 cells. The reduction of mitochondrial membrane potential, release of cytochrome c and SmacjDlABLO from mitochondria to cytosol, andtranslocation of AlF into nucleus were shown. While p53, p21 and $14-3-3{\gamma}$ were upregulated, cyclin Band cdc2 were downregulated by PEA treatment. Taken together, PEA induces apoptosis in chemoresistant YD-9 cells via activation of caspases, mitochondrial events and regulation of cell cycle genes.

Analysis of Archaeal Community in Autotrophic Perchlorate-degrading Enrichment Culture (독립영양 방식으로 퍼클로레이트를 분해하는 농화배양 내 고세균 군집 분석)

  • Kim, Young-Hwa;Do, Sanghyun;So, Hyunseung;Been, Junwon;Sung, Haechan;Ji, Sungchan;Son, Myunghwa;Ahn, Yeonghee
    • Journal of Life Science
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    • v.27 no.4
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    • pp.435-441
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    • 2017
  • Perchlorate ($ClO_4^-$) is an emerging contaminant detected in soil, groundwater, and surface water. Previous study revealed bacterial community in the enrichment culture tdegraded perchlorate using elemental sulfur as an electron donor. Quantitative and qualitative molecular methods were employed in this study to investigate archaeal community in the enrichment culture. Real-time qPCR showed that archaeal 16S rRNA gene copy number in the culture was about 1.5% of bacterial 16S rRNA gene copy number. This suggested that less archaea were adapted to the environment of the enrichment culture and bacteria were dominant. DGGE banding pattern revealed that archaeal community profile of the enrichment culture was different from that of the activated sludge used as an inoculum for the enrichment culture. The most dominant DGGE band of the enrichment culture was affiliated with Methanococci. Further research is necessary to investigate metabolic role of the dominant archaeal population to better understand microbial community in the perchlorate-reducing enrichment culture.

Recent Home Networking Services Development and Future Directions: Case analysis of Korean Smart Apartment Complexes (홈네트워킹 서비스 현황 및 발전 방향: 국내 사이버 타운 사례분석)

  • Sawng, Yeong-Wha;Han, Hyun-Soo
    • Information Systems Review
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    • v.6 no.2
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    • pp.269-284
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    • 2004
  • Induced from government policy to boost regional economic competitiveness, regional informatization forming e-community has been the subject included in the various regional informatization master plans in Korea. However, few cases are reported for its successful implementation mainly due to the lack of profitable business model to encourage investment. On the other hand, most efforts to build smart apartments, part of the home networking in a broad sense, has been pursued from the different directions. Telecommunication giant such as Korea Telecom tries to find new source of revenue exploiting enhanced broad band technology. Also, construction companies started constructing housing complexes equipped with built-in high speed network infrastructure as a means to differentiation to other competitors. The contents providing community portal has become mandatory in the sense of bearing the cost from customer side who are willing to adopt those services for new smart house. Our research motivation stems from exploring critical value aspects of realizing the profitability of this emerging new business model, that is, industry convergence model. In this paper, mainly from the survey results of the Korean smart apartment complexes, we reported recent home networking services development in Korea, and value propositions from the business model perspective. Merged business model components of telecommunications, construction, and internet contents are analyzed to provide the insights for future directions.

Characteristics and Pathogenicity of Hyphantria cunea Nuclear Polyhedrosis Virus (Hyphantria cunea Nuclear Polyhedrosis Virus의 특성 및 병원성)

  • Lee, Keun-Kwang;Kim, Myung-Kon;Park, Il-Woong
    • Applied Biological Chemistry
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    • v.38 no.5
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    • pp.435-439
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    • 1995
  • Some characteristics and pathogenicity of Hyphantria cunea nuclear polyhedrosis virus (HcNPV), a potential microbial pesticide was studied. H. cunea NPV replicated in the nucleus of S. frugiperda cells cultured in the TNMFH medium. In case of virus infected cell, prepolyhedra formation was observed at 24hrs post-infection. At 48 hrs post-infection, Most of the infected cell contained many mature polyhedra which were released into culture media 72 hrs post-infection, with the cells grown in suspension culture, pH of the culture medium increased during the virus replication: the pH of fresh medium was 6.35 and rose to 6.77 within 120 hrs. Polyhedra formed a band in linear density gradient of sucrose by centrifugation, which co-sedimented with $50{\sim}55%$ sucrose. The shape of the purified polyhedra was mostly tetragonal hexahedron and its size was about $2.5{\mu}m$. Electron microscopy and phase contrast microscopy showed that many bundled nucleocapsids were occluded in mature polyhedra at 48 hrs post infection. H. cunea larvae infected with NPV showed a higher motality in the second and third instar than in the fourth instar. Death rate of H. cunea larvae in the second and third instar fed with leaves coated with $1.5{\times}10^{9}{\sim}l.5{\times}10^{7}PIBs/ml$ reached more than 90%.

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Ontogenetic Color Variation of Abudefduf notatus (Pomacentridae: Perciformes) Revealed by 16S rRNA Sequences Analysis (미토콘드리아 16S rRNA 염기서열 분석에 의해 밝혀진 동갈자돔 치어의 성장에 따른 체색변이)

  • Song, Young Sun;Kim, Jin-Koo
    • Korean Journal of Ichthyology
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    • v.25 no.2
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    • pp.53-58
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    • 2013
  • Seven individuals (16.1~29.1 mm in length) which were estimated with fishes of genus Abudefduf were collected in Seogwipo, Jeju Island in the summer of 2011 and 2012. Among them, five individuals (20.8~29.1 mm SL) are similar to Abudefduf notatus, based on morphological characters such as yellow transverse band on the body, one small black spot on the base of the pectoral fin, and yellow caudal fin. On the other hand, two individuals (16.1 mm, 17.0 mm SL) differ from them in several light transverse bands and transparent rays of all fins except for pelvic fin, and anterior transparent head with only one (16.1 mm). According to the results of molecular analyses of 578 base pairs of mtDNA 16S rRNA sequences, these individuals correspond to A. notatus adult, with a high bootstrap value of 95% (genetic distance, d=0.002). Therefore, this study shows that the individuals more than 20.8 mm similar to adult body color but the 16.1 mm individual differs to that of adult. We confirm that this species changes to body color during their early life stages. This result regards as a survival strategy to protect themselves against their predator during their early life stages.