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Sex Determination of In Vitro Fertilized Bovine Embryos by Fluorescence in situ Hybridization Technique

  • Han, M. S.;E. J. Cho;H. B. Ha;Park, H. S.;S. H. Sohn
    • Proceedings of the KSAR Conference
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    • 2004.06a
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    • pp.287-287
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    • 2004
  • Sexing from bovine embryos which were fertilized in vitro implicate a possibility of production of the sex controlled cattle. This study was carried out to investigate the possibility of determining of embryo sex by fluorescence in situ hybridization (FISH) technique. FISH was achieved in in vitro fertilized bovine embryos using a bovine Y-specific DNA probe which constructed from the btDYZ-1 sequences. (omitted)

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Design on a monitoring controller with rapid charger for the electric vehicle based on CAN protocol (CAN 통신 기반 전기차 급속충전 모니터링 시스템 설계)

  • Choo, Yeon-Gyu;Lee, Kwang-Seok;Kim, Hyun-Deok;Kim, Bong-Gi;Kang, Sung-Soo;Kim, Sung-Du
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.10a
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    • pp.747-749
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    • 2010
  • 전기자동차 인프라 구축을 위해서는 급속충전관련 기술 확보 및 시설확충에 관한 투자와 연구가 지속적으로 이루어지고 있는 실정이다. 특히 다양한 급속충전 기술 중에서 공동 인프라용으로 적합한 방식에는 어떤 종류가 있는지 살펴보고, 급속충전시스템을 이용하는 전기자동차의 충전 상태, 기타 동작 상태를 실시간으로 확인하기 위해 BMS의 CAN 통신을 기반으로 하는 전기자동차 급속충전 모니터링 시스템을 설계하는 방법을 제안한다.

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A Study on Social Welfare Supporting Strategies for Rural Women;A Case of Jinju City (농업 ${\cdot}$ 농촌 환경변화에 따른 농촌여성 복지 지원 전략에 관한 연구;진주시를 중심으로)

  • Kim, Gyung-Mee;Park, Kyong-Cheol
    • Journal of Agricultural Extension & Community Development
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    • v.9 no.1
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    • pp.101-119
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    • 2002
  • The purpose of this study were to describe the problems of rural women in changing rural society and environment in Korea, to search for the development directions, and propose strategies for improving rural women's welfare. Related literatures and existing data were reviewed to investigate the present conditions, social-economic status, and economic activities of rural women in Jinju city, Gyeongsangnam-Do province. Jinju city had relatively higher percentage of farming people and rural women than other cities's of Gyeongsangnam-Do, and agriculture occupied important portion in industrial sector in the area. According to the Jinju 21C Vision Project, the city was planning to build eco-friendly environment and welfare rural society. To achieve the goals and objectives of the project, the following points should be considered: 1) Success of the project may depend on how to motivate and support rural women to participate actively in the project. 2) Jinju city should set up some practical strategies for improving the status of rural women who take key roles in environment and agriculture. 3) The city should establish strong support system and practical programs for rural women in building eco-friendly Jinju city.

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Fertilization and Tree Density Effects on Cellulose Decomposition in a Larix leptolepis Plantation

  • Kim, Choonsig;Kim, Oue-Ryong;Ahn, Hyun-Chul;Cho, Hyun-Seo;Choo, Gab-Chul;Park, Jae-Hyeon
    • The Korean Journal of Ecology
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    • v.25 no.6
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    • pp.399-403
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    • 2002
  • Cellulose mass loss by cellulose filter papers was measured for 3 time (35 days, 70 days, 105 days) incubation during the growing season (from May to September 2002) with different tree density and after fertilization in a Japanese larch (Larix leptolepis) plantation. Cellulose mass loss rates were significantly different between tree density types and fertilization treatments during the study periods. After 105 day incubation of cellulose filter paper, cellulose mass loss rates were significantly higher in the low tree density (70.1 $\%$) than in the high tree density (49.9$\%$). Cellulose mass loss rates averaged 62.8$\%$ in the fertilization and 58.9% in the unfertilization treatments during the same periods. However, cellulose mass loss was not significantly different between the forest floor and the mineral soil layer except for 35 day incubation. The results indicate that cellulose decomposition rates are a useful index to express differences in organic matter decomposition activity in different tree density and after fertilizer treatments.

Production of Cloned Korean Native Goat (Capra hircus) by Somatic Cell Nuclear Transfer

  • Park, H.S.;Jung, S.Y.;Kim, T.S.;Park, J.K.;Moon, T.S.;Hong, S.P.;Jin, J.I.;Lee, J.S.;Lee, J.H.;Sohn, S.H.;Lee, C.Y.;Moon, Y.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.4
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    • pp.487-495
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    • 2007
  • The objectives of the present study were to initiate cloning of Korean native goat by somatic cell nuclear transfer (NT) and to examine whether unovulated (follicular) oocytes can support the same developmental ability of NT embryos as ovulated (oviductal) oocytes after hCG injection in stimulated cycles of the goat. The in vivo-matured and immature oocytes were collected from the oviducts and follicles of superovulated does, respectively, and the immature oocytes were maturated in vitro. Ear skin fibroblasts derived from a 3-yr-old female Korean native goat were used as the donors of nuclei or karyoplasts. Following fusion, activation and in vitro culture to a 2- to 4-cell stage, 49 in vitro-derived and 105 in vivo-derived embryos were transferred to 6 and 17 recipient does, respectively. One doe and three does of the respective groups were identified as pregnant by ultrasonography on day 30 after embryo transfer. However, only one doe, which had received in vivo-derived embryos, delivered a normal female kid of 1.9 kg on d 149. The cloned kid gained more weight than her age-matched females as much as 87% during the first 4 mo after birth (17.7 vs. $9.4{\pm}0.8$ kg) and reached puberty at 6-mo age a few months earlier than normal female does. The telomere length of the kid, which was similar to that of the donor fibroblast at 2-mo age, decreased 8% between 2- and 7-mo ages. Moreover, at 7-mo age, she had 21% shorter telomere than her age-matched goats. To our knowledge, this is the first case in which a cloned animal born with a normal weight exhibited accelerated growth and development. The unusually rapid growth and development of the cloned goat may have resulted from SCNT-associated epigenetic reprogramming involving telomere shortening.

The Effect of Stocking Density on Stress Related Genes and Telomeric Length in Broiler Chickens

  • Beloor, J.;Kang, H.K.;Kim, Y.J.;Subramani, V.K.;Jang, I.S.;Sohn, S.H.;Moon, Yang Soo
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.4
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    • pp.437-443
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    • 2010
  • To be economically profitable, the poultry industry demands an increase in stocking density, which could adversely affect chicken welfare. The current study was performed to investigate the effect of stocking density on stress-related, heat shock protein genes (HSP70 and HSP90), 3-hydroxyl-3-methyl-glutaryl coenzyme A reductase (HMGCR) gene and telomere length in broiler chickens. Seven-day-old broiler chickens were housed at High (0.0578 $m^2$/bird), Standard (0.077 $m^2$/bird) and Low (0.116 $m^2$/bird) stocking densities with 8 replicates each until 35 d of age. The growth performance, such as body weight gain and average daily feed intake, was found to be significantly (p<0.05) higher in the Low density group, but these parameters did not show any difference between the High and Standard groups. Other growth performance, such as feed conversion ratio and final feed intake, showed no difference among the treated groups. The expression levels of HSP70 and HMGCR were found to be elevated with the increase of stocking density. The expression level of these genes was significantly (p<0.05) higher in the High density stocked group compared with the other groups, whereas the expression levels were not significantly different between the Low and Standard groups. The expression levels of HSP90 did not show any significant changes among the treated groups. The telomeric length of the birds housed in High density was reduced significantly (p<0.05) when compared to that of the birds in Low density. These results clearly indicate that birds stocked at high density show physiological adaptive changes indicative of stress at gene transcriptional and telomere levels.

Changes of Qualities in Aerobic Packed Ripening Pork Using a Korea Traditional Seasoning During Storage (전통양념으로 숙성하여 함기포장한 돼지고기의 저장 중 품질변화)

  • Jin, S.K.;Kim, I.S.;Hah, K.H.;Hur, S.J.;Lyou, H.J.;Park, K.H.;Bae, D.S.
    • Journal of Animal Science and Technology
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    • v.47 no.1
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    • pp.73-82
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    • 2005
  • This study was carried out to evaluate changes in the characteristics of the fermented pork using a Korean traditional seasonings. Biceps femoris were cut to cube(7 ${\times}$ 12 ${\times}$ 2 em) and three Korean traditional seasonings such as soybean paste(Tl), garlic paste(T2), red pepper paste(T3), were seasoned by the proportions of meat to each seasonings(l: 1), respectively. The seasoned samples were fermented at 0 $\pm$ 1 $^{\circ}C$ for 10 days. Sensory evaluation did not significantly differ between all treatments. The highest pH among treatments were shown in T2, wheres T3 showed the lowest value. The highest saccarinity was shown in T2, followed by T3. Salinity was shown to be higher in all treatments. Shear force value was the highest in T2 and T3. VBN and TBARS increased during storage. The total bacterial counts was highest of storage 21 days. E. coli. was higher in the order of T2 > T3 > TI. Lactobacilli spp. was higher in the order of T2 > T1 > T3.

The Historical and Cultural Landscape, and the Scenic Value of Mangjinsan Mountain in Jinju (진주(晉州) 망진산(望晉山)의 역사문화경관과 명승적 가치)

  • Kim, Se-Ho
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.40 no.3
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    • pp.10-19
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    • 2022
  • In this study, historical and cultural landscapes were reviewed focusing on Mangjinsan Mountain in Jinju, Gyeongsangnam-do, and the scenic value was examined through this. First, Mangjinsan Mountain was noted in history, as Ansan(案山, a mountain on the opposite side of a housing/grave site) in Jinju, where Bongsu(烽燧, the beacon fire station) is located. Information on Mangjinsan Mountain was gradually doubled due to its status, and the beacon fire station of Mangjinsan Mountain was in charge of defending the Jinju area. Mangjinsan Mountain was described as a symbolic landscape of Jinju. Regarding the etymology of Mangjinsan Mountain, Sung Yeo-Sin(成汝信) analyzed the geography of Jinju and suggested that it was a place name originating from the phoenix. However, looking at various records, it is confirmed that the name of Mangjinsan Mountain is maintained uniformly, but the inscription is not unified. Second, Mangjinsan Mountain became one of the major stage for the Japanese Invasion of Korea in 1592, and in 1597. It is confirmed to be a place which has joys and sorrows, for that it provided an opportunity to win the Siege of Jinju in 1592, but many casualties occurred in 1597. On the other hand, in the area of Mangjinsan Mountain Byeolseo(別墅), temples, and administrative facilities were located to establish cultural history of the time, and in the 19th century, Manggyeongdae Pavilion was built due to the scenic value of viewing Jinju Castle. These are examples of testimony how Mangjinsan Mountain has an important meaning in Jinju's history and culture. Third, in the late Joseon Dynasty, a poem reciting Mangjinsan Mountain appears, which shows that the Mountain has established itself as a scenic site in Jinju. The description of Mangjinsan Mountain is confirmed in the literature that lists the scenic sites of Jinju. On the other hand, writers who lived in Jinju paid attention to the beacon fire station, singing about the peaceful world without war and looking back the history. In the 19th century, Jeonbyeolyeon(a farewell party) was held, which seems to be the result of the beauty of viewing Jinju and overviewing the area. Through the facts, the symbolism and scenic value of Mangjinsan Mountain in Jinju were confirmed.

Influence of Essential Oil Components on Growth Performance and the Functional Activity of the Pancreas and Small Intestine in Broiler Chickens

  • Jang, I.S.;Ko, Y.H.;Yang, H.Y.;Ha, J.S.;Kim, J.Y.;Kim, J.Y.;Kang, S.Y.;Yoo, D.H.;Nam, D.S.;Kim, D.H.;Lee, C.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.3
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    • pp.394-400
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    • 2004
  • To investigate the efficacy of alternatives to antibiotics, the present study was conducted to compare the effects of antibiotic, lactic acid, a blend of commercial essential oils (EOs) and EOs in combination with lactic acid on growth performance and the functional activity of the gut in broiler chickens. A total of 168 broiler chickens were given the basal diet supplemented with 10 ppm colistin (T1), 0.1% lactic acid (T2), 25 ppm EOs (T3), 25 ppm EOs+0.1% lactic acid (T4), 50 ppm EOs (T5) or 50 ppm EOs+0.1% lactic acid (T6) in the period 3 to 35 days of age. As a result, the broiler chickens assigned to T4 group throughout the experimental period had apparently (p<0.05) greater body weight and total gain than these assigned to T1, T2, T3 and T5 groups. However, there was no difference in growth performance among the birds fed the diets supplemented with antibiotic (T1), lactic acid (T2) and EOs (T3 and T5) alone. The weights of digestive organs and the number of lactobacilli and E. coli in the lower ileum were not affected by dietary treatments. Total trypsin activity was significantly (p<0.05) greater in T4 than T1, T2, T3 and T5 groups. Total and specific pancreatic $\alpha$-amylase activities were significantly (p<0.05) enhanced in the broiler chickens fed T4 diet compared with these fed T1, T2 and T3 diets. However, there were no differences in growth performance and digestive enzyme activities including pancreatic trypsin and $\alpha$-amylase between T4 and T6 groups fed the diets supplemented with either low or high EOs levels in combination of lactic acid. In conclusion, a blend of commercial EOs combined with lactic acid showed significant increases in digestive enzyme activities of the pancreas and intestinal mucosa, leading to increase in growth performance.

A Comparison of Litterfall Dynamics in Three Coniferous Plantations of Identical Age under Similar Site Conditions

  • Jeong, Jae-Yeob;Kim, Choon-Sig;An, Hyun-Chul;Cho, Hyun-Seo;Choo, Gap-Chul
    • Journal of Ecology and Environment
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    • v.32 no.2
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    • pp.97-102
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    • 2009
  • This study was carried out to evaluate litterfall dynamics in three adjacent coniferous tree plantations (larch: Larix leptolepis; red pine: Pinus densiflora; rigitaeda pine: P. rigitaeda) planted in the same year (1963), and growing under similar environmental conditions in the Sambong Exhibition Forests, Hamyang-gun, Gyeongsangnam-do. Litter was collected monthly between July 2006 and June 2008. Needle, broad leaf and total litter inputs followed a similar monthly pattern in the three coniferous plantations. The amounts of needles, flowers, and miscellaneous litter were significantly lower in the larch than in the two pine plantations, while branch litter was significantly higher in the larch than in the two pine plantations. Average total litterfall for two years was significantly higher for the pine (5,475 kg $ha^{-1}\;yr^{-1}$ for red pine and 5,290 kg $ha^{-1}\;yr^{-1}$ for rigitaeda pine) plantations than for the larch (3,953 kg $ha^{-1}\;yr^{-1}$ plantation. Needle litter comprised about 73.1% of total litterfall for the rigitaeda pine, 70.8% for the red pine and 62.9% for the larch plantations. Our results demonstrate that litterfall inputs can be affected by tree species.